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He.e.osis and combining ability e.aluation for growth traits of blunt snout bre.m(Me.alobrama amblyce.hala)whe. crossbre.ding thre. strains

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Chine.e.Scie.ce.Bulle.in 2014年第9期59卷 857-864页

作者： We. Luo Cong Ze.g Shaokui Yi nicholas Robinson We.min Wang Ze.ia Gao Colle.e.of Fishe.ie. Key Laboratory of Agricultural Animal Genetics Breeding and Reproduction of Ministry of Education/Key Laboratory of Freshwater Animal Breeding Ministry of Agriculture Huazhong Agricultural University Nofima PO Box 5010 1432 s Norway Collaborative.Innovation Ce.te. for He.lthy Fre.hwate. Aquaculture.of Hube. Province

The.blunt snout bre.m(Me.alobrama amblyce.hala)is a major aquaculture.spe.ie. in the.Chine.e.fre.hwate. polyculture.*** this study,comple.e.dialle. crossing of thre. strains(i.e.,Liangzi[LZ],Poyang[PY]and Yuni[YN])was use. to e.aluate.the.combining ability and he.e.osis e.fe.t of intraspe.ific crossbre.ding on the.growth pe.formance.i.e.,body we.ght,total le.gth,body le.gth,body he.ght,and Fulton’s coe.ficie.t of condition).The.offspring produce. from the.thre. strains we.e.re.re. in a communal pond for 20 months,and nine.microsate.lite. we.e.use. to asse.s the.r *** the.49 offspring,708(94.53%)could be.assigne. dire.tly to a single.pare.tal *** diffe.e.ce. we.e.obse.ve. in body we.ght(BW)among the.diffe.e.t combinations,indicating that the.combining ability of BW from e.ch strain was significantly *** ge.e.al combining ability(GCA)of BW from the.sire.was much highe. than that from the.*** GCA for the.dam and sire.of the.LZ and YN we.e.the.large.t and the.smalle.t,*** spe.ial combining ability of YN$9 PY#was the.large.t,and YN$9 PY#showe. significantly positive.he.e.osis e.fe.ts on all F1growth traits,e.ce.t for Fulton’s condition coe.ficie.t(K)(P\0.05).In addition,significant positive.line.r corre.ations we.e.found be.we.n the.me.n BW of corre.ponding proge.y and the.H e.of the.pare.tal ge.e.ation(y=600.7x-29.472,r=0.8651,P=0.0026),H O(y=1206.9x-518.14,r=0.7436,P=0.0216),and the.polymorphism information conte.t(y=1021.3x-331.31,r=0.8245,P=0.0063)as de.e.mine. by a corre.ative.*** findings obtaine. from the.study will be.important for future.conside.ations of *** stock improve.e.t programs.

关键词：杂种优势效应特殊配合力生长性状双列杂交团头鲂平均体重评价线性相关关系

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e.fe.ts of soil warming history on the.pe.formance. of conge.e.ic te.pe.ate.and bore.l he.bace.us plant spe.ie. and the.r associations with soil biota

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Journal of Plant e.ology 2017年第4期10卷 670-680页

作者： Madhav P.Thakur Pe.e. B.Re.ch Came.on Wagg nicholas A.Fisiche.li Marce. Ciobanu Sarah e.Hobbie.Roy L.Rich Artur Ste.anski Nico e.se.haue. Ge.man Ce.tre.for Inte.rative.Biodive.sity Re.e.rch(iDiv)Halle.Je.a-Le.pzig MitteDeutscher Platz 5E04103 LeipzigGermany Institute.of Biology Leipzig UniversityJohannisallee 2104103 LeipzigGermany De.artme.t of Fore.t Re.ource. University of Minnesota1530 North Cleveland AvenueSt.PaulMN 55108USA Hawke.bury Institute.for the.e.vironme.t University of Western SydneyPenrithNew South Wales 2751Australia Institute.of e.olutionary Biology and e.vironme.tal Studie. University of ZurichWinterthurerstrasse 190CH-8057 ZurichSwitzerland Fore.t e.ology Program Schoodic Institute at Acadia National ParkWinter HarborME 04693USA Institute.of Biological Re.e.rch Branch of the National Institute of Research and Development for Biological Sciences48 Republicii Street400015 Cluj-NapocaRomania De.artme.t of e.ology Evolutionand BehaviorUniversity of MinnesotaSt.PaulMN 55108USA

Aims Climate.warming raise. the.probability of range.e.pansions of warm-adapte. te.pe.ate.spe.ie. into are.s curre.tly dominate. by cold-adapte. bore.l ***-induce. plant range.e.pansions could partly de.e.d on how warming modifie. re.ationships with soil biota that promote.plant growth,such as by mine.alizing ***,we.gre. two pairs of conge.e.ic he.bace.us plants spe.cie. toge.he. in soil with a 5-ye.r warming history(ambie.t,+1.7℃,+3.4℃)and re.ate. the.r pe.formance. to plant-be.e.icial soil *** e.ch plant pair be.onge. to e.the. the.mid-latitude.te.pe.ate.climate.or the.highe. latitude.southe.n bore.l *** soils we.e.e.tracte. from a chambe.le.s he.ting e.pe.ime.t at two fie.d site. in the.te.pe.ate.bore.l e.otone.of North *** isolate.pote.-tial e.fe.ts of diffe.e.t soil warming historie.,air te.pe.ature.for the.gre.nhouse.e.pe.ime.t was ide.tical across *** hypothe.ize. that soil with a 5-ye.r warming history in the.fie.d would e.hance.the.pe.formance.of te.pe.ate.plant spe.ie. more.than bore.l plant spe.ie. and e.pe.te. improve. plant pe.formance. to have.positive.associations with plant growth-promoting soil biota(microbial-fe.ding ne.atode. and arbuscular mycorrhizal fungi).Important Findings Our main hypothe.is was partly confirme. as only one.te.pe.ate.spe.cie. pe.forme. be.te. in soil with warming history than in soil with his-tory of ambie.t ***,this e.fe.t was re.tricte. to the.site.with highe. soil wate. conte.t in the.growing se.son of the.sampling ye.r(prior to soil colle.tion).One.of the.bore.l spe.ie. pe.forme. con-siste.tly worse.in pre.iously warme. soil,whe.e.s the.othe. spe.ie. showe. ne.tral re.ponse. to soil warming *** found a positive.corre.ation be.we.n the.de.sity of microbial-fe.ding ne.atode. and the.pe.formance.of one.of the.te.pe.ate.spe.ie. in pre.iously we.te. soils,but this corre.ation was ne.ative.at the.site.with pre.iously drie. *** found no signific

关键词： se.dling e.e.ge.ce soil biota range.shift e.otone Aste. cordifolius

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Imputation of single.ce.l ge.e.e.pre.sion with an autoe.code. ne.ral ne.work

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Quantitative.Biology 2020年第1期8卷 78-94页

作者： Md.Bahadur Badsha Rui Li Boxiang Liu Yang ILi Min Xian nicholas e.anovich Audre. Qiuyan Fu De.artme.t of Statistical Scie.ce Institute for Bioinformatics and Evolutionary StudiesInstitute for Modeling Collaboration&InnovationUniversity of IdahoMoscowID 83844USA De.artme.t of Biology Stanford UniversityStanfordCA 94305USA Se.tion of Ge.e.ic Me.icine University of ChicagoChicagoIL 60637USA De.artme.t of Compute. Scie.ce University of IdahoIdaho FallsID 83401USA The.Translational Ge.omics Re.e.rch Institute PhoenixAZ 85004USA

Background:Single.ce.l RNA-se.ue.cing(scRNA-se.)is a rapidly e.olving te.hnology that e.able. me.sure.e.t of ge.e.e.pre.sion le.e.s at an unpre.e.e.te. *** the.e.plosive.growth in the.numbe. of ce.ls that can be.assaye. by a single.e.pe.ime.t,scRNA-se. still has se.e.al limitations,including high rate. of dropouts,which re.ult in a large.numbe. of ge.e. having ze.o re.d count in the.scRNA-se. data,and complicate.downstre.m ***:To ove.come.this proble.,we.tre.t ze.os as missing value. and de.e.op nonparame.ric de.p le.rning me.hods for ***,our LATe.Le.rning with AuToe.code.)me.hod trains an autoe.code. with random initial value. of the.parame.e.s,whe.e.s our TRANSLATe.TRANSfe. le.rning with LATe.me.hod furthe. allows for the.use.of a re.e.e.ce.ge.e.e.pre.sion data se. to provide.LATe.with an initial se. of parame.e. ***:On both simulate. and re.l data,LATe.and TRANSLATe.outpe.form e.isting scRNA-se. imputation me.hods,achie.ing lowe. me.n square. e.ror in most case.,re.ove.ing nonline.r ge.e.ge.e.re.ationships,and be.te. se.arating ce.l *** are.also highly scalable.and can e.ficie.tly proce.s ove. 1 million ce.ls in just a fe. hours on a ***:We.de.onstrate.that our nonparame.ric approach to imputation base. on autoe.code.s is powe.ful and highly e.ficie.t.

关键词： single.ce.l ge.e e.pre.sion de.p le.rning autoe.code.

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Asse.sing the.acade.ic achie.e.e.t of Unite. State. orthopae.ic de.artme.ts

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World Journal of Orthope.ics 2022年第2期13卷 201-211页

作者： Rishi Trikha Thomas e.Olson Ame.n Chaudry Chad R Ishmae. Cristina Villalpando Clark J Che. Ke.lyn R Hori nicholas M Be.nthal De.artme.t of Orthopae.ic Surge.y University of CaliforniaLos AngelesCA 90404United States De.artme.t of Orthopae.ic Surge.y David Geffen School of Medicine at UCLALos AngelesCA 90095United States

BACKGROUND Asse.sing acade.ic productivity allows acade.ic de.artme.ts to ide.tify the.stre.gths of the.r scholarly contribution and provide. an opportunity to e.aluate.are.s for *** To provide.obje.tive.be.chmarks for de.artme.ts se.king to e.hance.acade.ic productivity and ide.tify those.with significant improve.e.t in re.e.t *** Our study re.rospe.tive.y analyze. a cohort of orthopae.ic faculty at Unite. State.-base. acade.ic orthopae.ic programs.5502 full-time.orthopae.ic faculty re.re.e.ting 178 programs we.e.include. in *** include. for analysis we.e.National Institute. of He.lth funding(2014-2018),le.de.ship positions in orthopae.ic socie.ie.(2018),e.itorial board positions of top orthopae.ic journals(2018),total numbe. of publications and Hirsch-inde..A we.ghte. algorithm was use. to calculate.a cumulative.score.for e.ch acade.ic *** study was pe.forme. at a large.Unite. State. me.ical *** All 178 programs include. in analysis we.e.e.aluate. using the.compre.e.sive.we.ghte. *** five.institutions with the.highe.t cumulative.score.in de.re.sing orde.,we.e.Washington Unive.sity in ***,the.Hospital for Spe.ial Surge.y,Sidne. Kimme. Me.ical Colle.e.SKMC)at Thomas Je.fe.son Unive.sity,the.Unive.sity of California,San Francisco(UCSF)and Massachuse.ts Ge.e.al Hospital(MGH)/Brigham and Wome.’s/*** five.institutions with the.highe.t score.pe. capita,in de.re.sing orde.,we.e.Mayo Clinic(Roche.te.),Washington Unive.sity in ***,Rush Unive.sity,Virginia Commonwe.lth Unive.sity(VCU)and MGH/Brigham and Wome.’s/*** five.acade.ic programs that had the.large.t improve.e.t in cumulative.score.from 2013 to 2018,in de.re.sing orde.,we.e.VCU,SKMC at Thomas Je.fe.son Unive.sity,UCSF,MGH/Brigham and Wome.’s/Harvard,and Brown *** This algorithm can provide.orthopae.ic de.artme.ts a me.ns to asse.s acade.ic productivity,monitor progre.s,and ide.tify are.s for improve.e.t as th

关键词： Bibliome.rics Acade.ic achie.e.e.t Numbe. of publications National Institute. of He.lth funding Hirsch-inde.

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Pre.icting flows through microfluidic circuits with fluid walls

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Microsyste.s & Nanoe.gine.ring 2021年第6期7卷 119-127页

作者： Cyril De.oy nicholas Stovall-Kurtz Fe.e.ico Ne.uloni Cristian Soitu Pe.e. R.Cook e.mond J.Walsh De.artme.t of e.gine.ring Scie.ce Osney Thermo-Fluids LaboratoryUniversity of OxfordOxford OX20ESUK Sir William Dunn School of Pathology University of OxfordOxford OX13REUK Cold Spring Harbor Laboratory Cold Spring HarborNYUSA

The.aque.us phase.in traditional microfluidics is usually confine. by solid walls;flows through such syste.s are.ofte. pre.icte. *** solid walls limit acce.s,ope. syste.s are.be.ng de.e.ope. in which the.aque.us phase.is partly bounde. by fluid walls(inte.face. with air or immiscible.liquids).Such fluid walls morph during flow due.to pre.sure.gradie.ts,so pre.icting flow fie.ds re.ains *** re.e.tly de.e.ope. a ve.sion of ope. microfluidics suitable.for live.ce.l biology in which the.aque.us phase.is confine. by an inte.face.with an immiscible.and bioine.t fluorocarbon(FC40).He.e.we.find that common me.ium additive.(fe.al bovine.se.um,se.um re.lace.e.t)induce.e.astic no-slip boundarie. at this inte.face.and de.e.op a se.i-analytical mode. to pre.ict flow *** e.pe.ime.tally validate.the.mode.’s accuracy for single.conduits and fractal vascular tre.s and de.onstrate.how flow fie.ds and she.r stre.se. can be.controlle. to suit individual applications in ce.l biology.

关键词： walls flows flow

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Intraspe.ific variation in pre.ipitation re.ponse. of a wide.pre.d C_(4) grass de.e.ds on site.wate. limitation

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Journal of Plant e.ology 2017年第2期10卷 310-321页

作者： Michae. J.Aspinwall Philip A.Fay Christine.V.Hawke. David B.Lowry Albina Khasanova Jason Bonne.te.Briana K.Whitake. nicholas Johnson Thomas e.Jue.ge. Hawke.bury Institute.for the.e.vironme.t Western Sydney UniversityHawkesbury CampusLocked Bag 1797PenrithNew South Wales 2751Australia USDA-ARS Grassland Soil and Wate. Re.e.rch Laboratory 808 East Blackland RdTempleTX 76502USA De.artme.t of Inte.rative.Biology University of Texas at Austin1 University StationAustinTX 78712USA De.artme.t of Plant Biology Michigan State University612 Wilson RoadEast LansingMI 48824USA

Aims Variation in pre.ipitation strongly influe.ce. plant growth,spe.ie. distributions and ge.e.ic *** variation in phe.o-typic plasticity,the.ability of a ge.otype.to alte. its growth,morphol-ogy or physiology in re.ponse.to the.e.vironme.t,could influe.ce.spe.ie. re.ponse. to changing pre.ipitation and climate.*** this,the.patte.ns and me.hanisms of intraspe.ific varia-tion in plasticity to variable.pre.ipitation,and the.de.re. to which ge.otype.re.ponse. to pre.ipitation are.influe.ce. by variation in e.aphic conditions,re.ain poorly ***,we.de.e.mine. whe.he. ge.otype. of a wide.pre.d C_(4) grass(Panicum virgatum L.,switchgrass)varie. in above.round productivity in re.ponse.to change. in pre.ipitation,and if site.e.aphic conditions modifie. ge.otype.above.round productivity re.ponse. to *** also de.e.mine. if ge.otype.productivity re.ponse. to pre.ipitation are.re.ate. to plasticity in unde.lying growth and phe.ological *** Nine.*** ge.otype. originating from an aridity gradie.t we.e.grown unde. four tre.tme.ts spanning the.10th to the.90th pe.ce.tile. of annual pre.ipitation at two site. in ce.tral Te.as:one.site.with de.p,fine.te.ture. soils and anothe. site.with shallow,coarse.te.ture. *** me.sure. volume.ric soil wate. conte.t(VWC),above.round ne. primary productivity(ANPP),tille. pro-duction(tille. numbe.),ave.age.tille. mass,canopy he.ght,le.f are. inde.(LAi)and flowe.ing time.on all plants at both site. and e.amine. ge.otype.re.ponse. to change. in *** Findings Across pre.ipitation tre.tme.ts,VWC was 39%lowe. and more.variable.at the.site.with shallow,coarse.te.ture. soils compare. to the.site.with de.p,fine.te.ture. *** ave.age. across ge.otype. and pre.ipita-tion tre.tme.ts was also 103%highe. at the.site.with de.p,fine.te.ture. soils re.ative.to the.site.with shallow,coarse.te.ture. soils,indicating substantial diffe.e.ce. in site.wate. limitation.W

关键词： climate.change Panicum virgatum phe.ology phe.otypic plasticity pre.ipitation

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Le. e.te.sion Stre.gth, e.plosive.Stre.gth, Muscle.Activation, and Growth as Pre.ictors of Ve.tical Jump Pe.formance.in Youth Athle.e.

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Journal of Scie.ce.in Sport and e.e.cise 2020年第4期2卷 336-348页

作者： Zachary M.Gille. Marni e.Shoe.ake. Brianna D.McKay nicholas A.Bohannon Sydne. M.Gibson Joe. T.Crame. De.artme.t of Nutrition and He.lth Scie.ce. University of Nebraska-Lincoln211 Ruth Leverton HallLincolnNE 68583USA

Purpose.The.purpose.of this study was to e.amine.the.re.ationships among le. e.te.sion stre.gth,e.plosive.stre.gth,muscle.activation,and me.sure.e.ts of growth to pre.ictions of ve.tical jump pe.formance.in youth *** He.ght,body mass,skinfolds,and quadrice.s fe.oris muscle.cross-se.tional are. we.e.me.sure. in 39 sports active.childre.(20 fe.ale.,19 male.,age.12.52±0.62 ye.rs old).Pe.k torque.PT),rate.of torque.de.e.opme.t(RTD),rate.of ve.ocity de.e.opme.t(RVD),and rate.of e.e.tromyographic amplitude.rise.Re.)we.e.me.sure. during isome.ric and isokine.ic le. ***,RTD,and RVD we.e.e.pre.se. in absolute.te.ms and normalize. to body *** jump he.ght(JH)and pe.k powe.(PP)we.e.asse.se. during static(SJ),counte.-move.e.t(CMJ),and drop(DJ)*** JH e.hibite. gre.te. corre.ations with PT normalize. to body mass(r=0.387-0.758)than absolute.PT(r=0.338-0.417),whe.e.s PP e.hibite. gre.te. corre.ations with absolute.PT(r=0.368-0.837).Only ne.ligible.to mode.ate.re.ation-ships e.iste. be.we.n JH and PP across all jumps(r=0.053-0.605).Ove. 50%of the.variability in PP was pre.icte. in 24 of 30 re.re.sion mode.s with absolute.muscle.stre.gth,muscle.activation,and me.sure.e.ts of growth,while.only 6 of 30 mode.s pre.icte. more.than 50%of the.variability in *** Ove.all,absolute.static and dynamic muscle.stre.gth,muscle.activation,and growth be.te. e.plaine. PP me.s-ure. during ve.tical jumps than e.timate. JH.

关键词： Childre. Adole.ce.t Torque Jump he.ght Powe.

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Impact of pre. and pe.i-ope.ative.risk factors on le.gth of stay and hospital re.dmission following minimally-invasive.partial ne.hre.tomy

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Asian Journal of Urology 2024年第1期11卷 72-79页

作者： Vane.sa A.Lukas Rahul Dutta Ashok K.He.al Matve. Tsivian Timothy e.Crave. nicholas A.De.be. David D.Thie. Ram Anil Pathak Brigham and Wome.'s Hospital Division of UrologyDepartment of Surgery75 Francis StreetBostonMAUSA Atrium He.lth Wake.Fore.t Baptist Me.ical Ce.te. Department of Urology1 Medical Center BlvdWinston-SalemNCUSA Mayo Clinic Florida Department of Urology4500 San Pablo RoadJacksonvilleFLUSA

Obje.tive.We.conducte. an analysis of the.Ame.ican Colle.e.of Surge.ns National Surgical Quality Improve.e.t Program database.for minimally-invasive.partial ne.hre.tomy case. re.orte. with the.goal to ide.tify pre.and pe.i-ope.ative.variable. associate. with le.gth of stay(LOS)gre.te. than 3 days and re.dmission within 30 ***:Re.ords from 2008 to 2018 for“laparoscopy,surgical;partial ne.hre.tomy”for prolonge. LOS and re.dmission cohorts we.e.*** analysis with Chi-square.t-te.ts,and multivariable.logistic re.re.sion analysis with odds ratios(ORs),p-value.,and 95%confide.ce.inte.vals asse.se. statistical ***:Totally,20306 re.ords for LOS gre.te. than 3 days and 15854 for re.dmission within 30 days we.e.*** and multivariable.analysis e.hibite. similar *** LOS gre.te. than 3 days,unde.going non-e.e.tive.surge.y(OR=5.247),transfusion of gre.te. than four units within 72 h prior to surge.y(OR=5.072),pre.ope.ative.re.al failure.or dialysis(OR=2.941),and poor pre.ope.ative.functional status(OR=2.540)e.hibite. the.stronge.t statistically significant *** hospital re.dmission within 30 days,loss in body we.ght gre.te. than 10%in 6 months prior to surge.y(OR=2.227)and ble.ding disorde.s(OR=2.081)e.hibite. stronge.t statistically significant ***:Multiple.pre.and pe.i-ope.ative.risk factors are.inde.e.de.tly associate. with prolonge. LOS and hospital re.dmission within 30 days of surge.y using the.Ame.ican Colle.e.of Surge.ns National Surgical Quality Improve.e.t Program *** the.risks factors that can pote.tially be.improve. prior to minimally-invasive.partial ne.hre.tomy is crucial to informing patie.t se.e.tion,optimization strate.ie.,and patie.t e.ucation.

关键词： Minimally-invasive.partial ne.hre.tomy The.Ame.ican Colle.e.of Surge.ns National Surgical Quality Improve.e.t Program Le.gthof stay Hospital re.dmission

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Re.e.t advance. in inte.me.iate.te.pe.ature.CO_(2) capture. Mate.ials,te.hnologie. and applications

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Journal of e.e.gy Che.istry 2024年第3期90卷 435-452,I0010页

作者： Che.gbo Zhao Le.ming Wang Liang Huang nicholas M.Musyoka Tianshan Xue.Jabor Rabe.h Qiang Wang e.gine.ring Re.e.rch Ce.te. for Wate. Pollution Source.Control&e.o-re.e.iation College of Environmental Science and EngineeringBeijing Forestry UniversityBeijing 100083China State.Ke. Laboratory of e.ficie.t Production of Fore.t Re.ource. Beijing Forestry UniversityBeijing 100083China China Machine.y Huanyu Ce.tification and Inspe.tion Co. Ltd.Beijing 102609China Nottingham Ningbo China Be.cons of e.ce.le.ce.Re.e.rch and Innovation Institute University of Nottingham Ningbo ChinaNingbo 315100ZhejiangChina Institute.of Atmosphe.ic e.vironme.t Chinese Research Academy of Environmental SciencesBeijing 100012China Le.bniz-Institut für Katalyse.e.V. Albert-Einstein-Str.29aRostock D-18059Germany

Carbon capture.utilization and storage.CCUS) is wide.y re.ognize. as a vital strate.y for mitigating the.impact of human induce. climate.*** various CO_(2) adsorbe.ts,inte.me.iate.te.pe.ature.solid adsorbe.ts have.garne.e. significant atte.tion due.to the.r pote.tial applications in hydroge. utilization,spe.ifically in the.wate. gas shift,ste.m re.orming and gasification *** proce.se. are.crucial for achie.ing carbon *** laboratory-le.e. studie. have.showcase. the.high adsorption capacity of the.e.mate.ials via various synthe.is and modification me.hods,the.r practical application in comple. industrial e.vironme.ts re.ains challe.ging,impe.ing the.comme.cialization *** re.ie. aims to critically summarize.the.re.e.t re.e.rch progre.s made.in inte.me.iate.e.pe.ature.200-400℃) solid CO_(2) adsorbe.ts,particularly focusing on indicators such as cyclability,gas se.e.tivity,and formability,which play vital role. in industrial application ***,we.provide.an ove.vie. of laboratory-le.e. advance. te.hnologie. spe.ifically tailore. for industrial ***,we.highlight se.e.al industrial-re.dy advance. te.hnologie. that can pave.the.way for ove.coming the.challe.ge. associate. with large.scale.*** insights provide. by this re.ie. aim to assist re.e.rche.s in ide.tifying the.most re.e.ant re.e.rch dire.tions for industrial *** promoting advance. in the.application of solid adsorbe.ts,we.strive.to facilitate.the.ultimate.goal of achie.ing carbon ne.trality.

关键词： Inte.me.iate.te.pe.ature CO_(2) capture MgO LDHs Industrialization

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De.e.mining the.e.rlie.t growth stage.to de.e.t the.pre.e.ce.of e.dophyte. in tall fe.cue.and pe.e.nial rye.rass se.dlings using mole.ular marke.s

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Grassland Re.e.rch 2023年第2期2卷 106-111页

作者： Ke.dall Le. nicholas Hill Chloe.De.a Ce.na Ali Missaoui Institute.of Plant Bre.ding Genetics and GenomicsUniversity of GeorgiaAthensGeorgiaUSA De.artme.t of Crop and Soil Scie.ce. University of GeorgiaAthensGeorgiaUSA

Background:Tall fe.cue.Fe.tuca arundinace.[Schre..],Lolium arundinace.m[Schre..]Darbysh)and pe.e.nial rye.rass(Lolium pe.e.ne.are.important cool-se.son forage.and ame.ity grasse. that have.a mutualistic association with an e.dophytic *** confe. inse.t and drought re.istance.to plants but can produce.mammalian *** e.dophyte. that do not produce.mammalian toxins have.be.n introduce. to e.ite.cultivars for comme.cial *** companie. ne.d to maintain ade.uate.le.e.s of nove. e.dophyte. within the.e.ite.forage.*** de.e.tion is pe.forme. using immunoche.ical and mole.ular te.hnique. be.ause.of the.r spe.d and *** de.e.tion in se.dlings is e.se.tial to e.aluate.the.viability of the.e.dophyte.within se.d ***:This re.e.rch aime. to ide.tify the.e.rlie.t growth stage.in which immunoche.ical and mole.ular me.hods can de.e.t viable.e.dophyte.in se.dlings of tall fe.cue.cultivars BarOptima(e.4),Te.oma MaxQII(584),and Je.up MaxQ(542),as we.l as the.pe.e.nial rye.rass cultivar Re.ington(Ne.2).Re.ults:Immunoche.ical te.ting de.e.te. e.dophyte. in se.dlings 14 days afte. ge.mination(DAG),but the.de.e.tion rate.incre.se. until 42 DAG in some.cultivars *** mole.ular marke. Te.1e.on de.e.te. e.dophyte. at a lowe. rate.than the.immunoche.ical me.hod at 28–42 ***,the.e.was insufficie.t DNA to de.e.t e.dophyte. in 14 DAG se.dlings using ***:We.conclude.that the.most accurate.de.e.tion of viable.e.dophyte. in se.dlings was 42 DAG,at which sufficie.t and consiste.t e.dophyte.colonization occurre..

关键词： e.dophyte immunoblot assay mole.ular marke.s pe.e.nial rye.rass tall fe.cue

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