Determining the earliest growth stage to detect the presence of endophytes in tall fescue and perennial ryegrass seedlings using molecular markers
作者机构：Institute of Plant BreedingGenetics and GenomicsUniversity of GeorgiaAthensGeorgiaUSA Department of Crop and Soil SciencesUniversity of GeorgiaAthensGeorgiaUSA
出 版 物：《Grassland Research》 (草地研究（英文）)
年 卷 期：2023年第2卷第2期
页 面：106-111页
学科分类：090503[农学-草业科学] 0909[农学-草学] 0905[农学-畜牧学] 09[农学] 0903[农学-农业资源与环境]
基 金：The University of Georgia Cultivar Research and Development Fund
主 题：endophyte immunoblot assay molecular markers perennial ryegrass tall fescue
摘 要：Background:Tall fescue(Festuca arundinacea[Schreb.],Lolium arundinaceum[Schreb.]Darbysh)and perennial ryegrass(Lolium perenne)are important cool-season forage and amenity grasses that have a mutualistic association with an endophytic *** confer insect and drought resistance to plants but can produce mammalian *** endophytes that do not produce mammalian toxins have been introduced to elite cultivars for commercial *** companies need to maintain adequate levels of novel endophytes within the elite forage *** detection is performed using immunochemical and molecular techniques because of their speed and *** detection in seedlings is essential to evaluate the viability of the endophyte within seed ***:This research aimed to identify the earliest growth stage in which immunochemical and molecular methods can detect viable endophyte in seedlings of tall fescue cultivars BarOptima(e34),Texoma MaxQII(584),and Jesup MaxQ(542),as well as the perennial ryegrass cultivar Remington(NEA2).Results:Immunochemical testing detected endophytes in seedlings 14 days after germination(DAG),but the detection rate increased until 42 DAG in some cultivars *** molecular marker Tef1exon detected endophytes at a lower rate than the immunochemical method at 28–42 ***,there was insufficient DNA to detect endophytes in 14 DAG seedlings using ***:We conclude that the most accurate detection of viable endophytes in seedlings was 42 DAG,at which sufficient and consistent endophyte colonization occurred.
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