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不同南瓜砧木对小果型西瓜生长和果实品质的影响

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中国瓜菜 2024年第8期37卷 82-91页

作者：李帅宋宇张春雨 kaleem muhammad Mohsin 林昕原别之龙华中农业大学园艺林学学院武汉430070

近年来小果型西瓜的栽培面积增长迅速,为筛选出适宜小果型西瓜嫁接的南瓜砧木,以17个南瓜砧木和2个商业南瓜砧木(丰乐金甲、京欣砧4号)作为嫁接砧木,以小果型西瓜品种京美2K和武农8号作为接穗,研究不同南瓜砧木嫁接对西瓜嫁接苗成活率... 详细信息

近年来小果型西瓜的栽培面积增长迅速,为筛选出适宜小果型西瓜嫁接的南瓜砧木,以17个南瓜砧木和2个商业南瓜砧木(丰乐金甲、京欣砧4号)作为嫁接砧木,以小果型西瓜品种京美2K和武农8号作为接穗,研究不同南瓜砧木嫁接对西瓜嫁接苗成活率、定植成活率、植株田间长势、开花坐果节位、果实品质和产量的影响。结果表明,参试砧木均与小果型西瓜具有较高的亲和性;嫁接小果型西瓜的田间长势和产量优于自根栽培;除砧木S6和S26外,其他砧木嫁接不会显著改变西瓜果形指数;在内在品质指标中,春季以S12、S19、S5、S16、S13、S7和S17表现良好,秋季以S16、S19和S7表现最优。综合春秋两季的试验结果,S16、S19和S7这3个南瓜砧木优于2个商业砧木,适宜作为小果型西瓜的嫁接砧木。

关键词：小果型西瓜南瓜砧木生长品质产量

Molecular and in vitro biochemical assessment of chemosensory protein 10 from brown planthopper Nilaparvata lugens at acidic pH

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Journal of Integrative Agriculture 2022年第3期21卷 781-796页

作者： muhammad Irfan WARIS Aneela YOUNAS Rana muhammad kaleem ULLAH Fatima RASOOL muhammad Muzammal ADEEL WANG Man-qun College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070P.R.China National Center for Bioinformatics Quaid-i-Azam UniversityIslamaba 45320Pakistan College of Informatics Huazhong Agricultural UniversityWuhan 430070P.R.China

Chemosensory proteins(CSPs)are important molecular components of the insect olfactory system,which are involved in capturing,binding,and transporting hydrophobic odour molecules across the sensillum in sensillar lymph in regulating insect *** protein family(CSPs)is also involved in many other systems that are not linked to olfactory receptors in olfactory *** brown planthopper(BPH)is a monophagous pest of rice that causes damage by sucking phloem sap and transmitting a number of diseases caused by *** this study,fluorescence competitive binding assay and fluorescence quenching assay at acidic p H were performed as well as homology modelling to describe the binding affinity of Nlug *** competitive binding assay(FCBA)demonstrated that Nlug CSP10 bound strongly to nonadecane,farnesene,and 2-tridecanone at acidic p *** results of FCBA indicated that Nlug CSP10 bound different ligands at the physiological p H(5.0)of the bulk sensillum *** quenching assay demonstrated that Nlug CSP10 generated a stable complex with 2-tridecanone,while two ligands nonadecane and farnesene collided due to molecular *** interaction of selected ligands with the modelled structure of Nlug CSP10 was also analyzed,which found the key amino acids(Gln23,Gln24,Gln25,Asn27,Met33,Ser34,Ile35,Tyr36,Asn42,Met43,Val45,Asn46,Asn93,Arg96,Ala97,Lys99,and Ala100)in Nlug CSP10 that were involved in binding of volatile *** present study contributes to the binding profile of Nlug CSP10 that promotes the development of behaviorally active ligands based on BPH olfactory system.

关键词： insect olfaction chemosensory protein Nilaparvata lugens fluorescence competitive binding assay fluorescence quenching assay molecular docking

Genome-wide identification and spatiotemporal expression profiling of zinc finger SWIM domain-containing protein family genes

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Zoological Research 2023年第3期44卷 663-674页

作者： Imtiaz Ul Hassan Hafiz Mamoon Rehman Ziran Liu Liangji Zhou muhammad kaleem Samma Chengdong Wang Zixin Rong Xufeng Qi Dongqing Cai Hui Zhao Key Laboratory for Regenerative Medicine Ministry of EducationSchool of Biomedical SciencesFaculty of MedicineThe Chinese University of Hong KongHong Kong SARChina Centre for Agricultural Biochemistry and Biotechnology(CABB) University of Agriculture FaisalabadFaisalabad 38000Pakistan Qingdao Municipal Center for Disease Control and Prevention QingdaoShandong 266034China Key Laboratory of Regenerative Medicine of Ministry of Education Department of Developmental&Regenerative BiologyJinan UniversityGuangzhou 511436China Department of Biology and Chemistry City University of Hong KongHong Kong SARChina Department of Gene Technology School of Engineering Sciences in ChemistryBiotechnology and Health(CBH)KTH Royal Institute of Technology10691 StockholmSweden Kunming Institute of Zoology-The Chinese University of Hong Kong(KIZ-CUHK)Joint Laboratory of Bioresources and Molecular Research of Common Diseases Hong Kong SARChina Hong Kong Branch of CAS Center for Excellence in Animal Evolution and Genetics The Chinese University of Hong KongHong Kong SARChina

The biological function of the novel zinc-finger SWIM domain-containing protein family(ZSWIM)during embryonic development remains ***,we conducted a genome-wide analysis to explore the evolutionary processes of the ZSWIM gene family members in mice,Xenopus tropicalis,zebrafish,and *** identified nine putative ZSWIM genes in the human and mouse genome,eight in the Xenopus genome,and five in the zebrafish *** on multiple sequence alignment,three members,ZSWIM5,ZSWIM6,and ZSWIM8,demonstrated the highest homology across all four *** available RNA sequencing(RNAseq)data,ZSWIM genes were found to be widely expressed across different tissues,with distinct tissuespecific *** identify the functions of the ZSWIM protein family during embryogenesis,we examined temporal and spatial expression patterns of zswim family genes in Xenopus *** real-time polymerase chain reaction(qRT-PCR)revealed that each member had a distinct expression ***-mount in situ hybridization showed that both zswim1 and zswim3 were maternally expressed genes;zswim5 and zswim6were expressed throughout embryogenesis and displayed dynamic expression in the brain,eyes,somite,and bronchial arch at the late tailbud stages;zswim7 was detected in the eye area;zswim8 showed a dynamic expression pattern during the tailbud stages,with expression detected in the brain,eyes,and somite;zswim9 was faintly expressed throughout embryonic *** study provides a foundation for future research to delineate the functions of ZSWIM gene members.

关键词： ZSWIM gene family Xenopus Gene expression Phylogenetic analysis Bioinformatics

Disintegration of uncertainties associated with real-time multi-satellite precipitation products in diverse topographic and climatic area in Pakistan

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Journal of Mountain Science 2021年第3期18卷 716-734页

作者： muhammad MASOOD Ghulam NABI muhammad BABUR Aftab Hussain AZHAR muhammad kaleem ULLAH Centre of Excellence in Water Resources Engineering University of Engineering&Technology GT-RoadLahore 54890Pakistan Civil Engineering Department University of Central PunjabLahore 54782Pakistan Directorate of Hydrology Pakistan Water&Power Development Authority(WAPDA)MDO-WAPDAMangla Dam10200Pakistan Civil Engineering Department University of LahoreLahore 54000Pakistan

Satellite-based Precipitation Estimates(SPEs)have gained importance due to enhanced spatial and temporal resolution,particularly in Indus basin,where raingauge network has fewer observation stations and drainage area is laying in many *** of SPEs is based on indirect mechanism,therefore,assessment and correction of associated uncertainties is *** the present study,disintegration of uncertainties associated with four prominent real time SPEs,IMERG,TMPA,CMORPH and PERSIANN has been conducted at grid level,regional scale,and summarized in terms of regions as well as whole study area *** bias has been disintegrated into hit,missed,false biases,and Root Mean Square Error(RMSE)into systematic and random errors.A comparison among gauge-and satellite-based precipitation estimates at annual scale,showed promising result,encouraging use of real time SPEs in the study *** grid basis,at daily scale,from box plots,the median values of total bias(-0.5 to 0.5 mm)of the used SPEs were also encouraging although some under/over estimations were noted in terms of hit bias(-0.15 to 0.05 mm/day).Relatively higher values of missed(0.3 to 0.5 mm/day)and false(0.5 to 0.7 mm/day)biases were *** detected average daily RMSE,systematic errors,and random errors were also comparatively ***-scale spatial distribution of uncertainties revealed lower values of uncertainties in plain areas,depicting the better performance of satellite-based products in these ***,in areas of high altitude(>4000 m),due to complex topography and climatic conditions(orographic precipitation and glaciated peaks)higher values of biases and errors were *** barriers and point scale gauge data could also be a cause of poor performance of SPEs in these areas,where precipitation is more on ridges and less in valleys where gauge stations are usually *** system’s size and intensity can also be a reason of higher biases,because M

关键词： Satellite precipitation Real-time Error characterization IMERG Indus Basin

The role of chemosensory protein 10 in the detection of behaviorally active compounds in brown planthopper, Nilaparvata lugens

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Insect Science 2020年第3期27卷 531-544页

作者： muhammad Irfan Waris Aneela Younas muhammad Muzammal Adeel Shuang-Gang Duan Sundas Rana Quershi Rana muhammad kaleem Ullah Man-Qun Wang Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina College of informatics Huazhong Agricultural UniversityWuhanChina

Chemosensory proteins(CSPs)play important roles in insects’chemoreception,although their specific functional roles have not been fully *** this study,we conducted the developmental expression patterns and competitive binding assay as well as knock‐down assay by RNA interference both in vitro and in vivo to reveal the function of NlugCSP10 from the brown planthopper(BPH),Nilaparvata lugens(Stål),a major pest in rice *** results showed that NlugCSP10 messenger RNA was significantly higher in males than in females and correlated to gender,development and wing *** fluorescence binding assays revealed that NlugCSP10 exhibited the highest binding affinity with cis‐3‐hexenyl acetate,eicosane,and(+)‐β‐*** assay revealed that eicosane displayed attractant activity,while cis‐3‐hexenyl acetate,similar to(+)‐β‐pinene significantly repelled *** *** of NlugCSP10,which is responsible for cis‐3‐hexenyl acetate binding,significantly disrupted cis‐3‐hexenyl acetate ***,findings of the present study showed that NlugCSP10 could selectively interrelate with numerous volatiles emitted from host plants and these ligands could be designated to develop slow‐release mediators that attract/repel *** and subsequently improve the exploration of plans to control this insect pest.

关键词： behavioral responses chemosensory proteins expression patterns fluorescence competitive binding assays RNA interference

Effect of regional factor productivity on manufacturing sector: The case of Sino-Pak economic ties

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Financial Innovation 2020年第1期6卷 95-108页

作者： muhammad Imran An HuSen muhammad kaleem Arshad Khan Bangash Nizam Ud Din Sobia Department of Economics Bacha Khan UniversityCharsaddaPakistan School of Economics Nankai UniversitTianjinChina Department of Sociology and Gender Studies Bacha Khan UniversityCharsaddaPakistan Department of Business Administration Karakoram International UniversityGilgitPakistan Government Postgraduate Jahanzeb College SwatPakistan

Introduction:Economic ties and trade blocs increase the flow of trade between participating countries and lead to different levels of economic and structural *** description:This paper focuses on the structure of industrial value-added between China and Pakistan,as the two countries recently launched the CPEC(China-Pakistan Economic Corridor)*** and evaluation:This study utilizes panel data from 1995 to 2015 to test certain factor effects on regional value-added through econometric *** results show that each country has its own economic growth rate and market size that respond differently to industrial value-added ***:Aggregate factor productivity at China is higher than in Pakistan but growth in factor productivity in the latter is higher than the former;similarly,the share of bilateral trade is higher in the case of *** each country responds differently to the new economic ties,the macroeconomic results support bilateral economic ties between them.

关键词： New economic geography China-Pakistan economic-ties Factor productivities

Weathering and accumulation of trace elements in the soils of the Porali Plain, Balochistan: repercussions in agriculture

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Acta Geochimica 2024年第2期43卷 214-234页

作者： Maria kaleem Erum Bashir Shahid Naseem Tahir Rafique Bushra Shahab Department of Geology University of KarachiKarachi 75270Pakistan Applied Chemistry Research Centre PCSIR Laboratories ComplexKarachi 75280Pakistan

This study is theﬁrst attempt to assess the nature of the soil,especially on the western side of the Porali Plain in Balochistan;a new emerging agriculture hub,using weathering and pollution indices supplemented by multi-variate analysis based on geochemical *** outcomes of this study are expected to help farmers in soil manage-ment and selecting suitable crops for the ***-ﬁve soil samples were collected,mainly from the arable land of the Porali *** drying and coning-quarter-ing,soil samples were analyzed for major and trace ele-ments using the XRF technique;sieving and hydrometric methods were employed for granulometric ***-mated data were analyzed using Excel,SPSS,and Surfer software to calculate various indices,correlation matrix,and spatial *** granulometric analysis showed that 76%of the samples belonged to loam types of soil,12%to sand type,and 8%to silt *** indices:CIA,CIW,PIA,PWI,WIP,CIX,and ICV were calculated to infer the level of *** indices reﬂect mod-erate to intense weathering;supported by K_(2)O/AI_(2)O_(3),Rb/K_(2)O,Rb/Ti,and Rb/Sr *** of the geo-ac-cumulation and Nemerow Pollution indices pinpoint rela-tively high concentrations of Pb,Ni,and Cr concentration in the *** correlation matrix and Principal Compo-nent Analysis show that the soil in this study area is mainly derived from the weathering of igneous rocks of Bela Ophiolite(Cretaceous age)and Jurassic sedimentary rocks of Mor Range having SEDEX/MVT type *** may result in the undesirable accumulation of certain trace elements which adversely affects crops.

关键词： Weathering indices Pollution indices Accumulation Repercussions Trace elements Multivariate analyses Porali Plain Balochistan

Luminous composite ultrathin films of CdTe quantum dots/silk fibroin co-assembled with layered doubled hydroxide:Enhanced photoluminescence and biosensor application

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Journal of Materiomics 2018年第2期4卷 165-171页

作者： muhammad Sohail Haroone Ling Li Aftab Ahmad Yaping Huang Ruili Ma Ping Zhang Yuehua Hu Qari muhammad kaleem Jun Lu State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical TechnologyBeijing100029China CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijing100101China Beijing Advanced Innovation Center for Soft Matter Science and Engineering Beijing University of Chemical TechnologyP.Box 98Beisanhuan East Road 15Beijing100029China

Quantum dots(QDs)luminescent films are extensively applied to optoelectronics and optical ***,QDs aggregation results in the quenching of their fluorescence property which limits their practical applications to a greater *** order to resolve this issue,3-mercaptopropionic acid(3-MPA)functionalized Cadmium Tellurium(CdTe)QDs were stabilized by silk fibroin(SB)and coassembled with layered doubled hydroxide(LDH)to form(QDs@SF/LDH)_(n)ultrathin films(UTFs)via the layer-by-layer(LBL)***-Vis absorption and fluorescence spectroscopy showed a stepwise and normal growth of the films upon increasing the number of deposition *** and AFM studies confirmed the formation of a periodic layered structure and regular surface morphology of the thin *** compared to(CdTe QDs/LDH)_(n)UTFs,the(CdTe QDs@SF/LDH)_(n)UTFs displayed fluorescence enhancement and longer fluorescent lifetime,both in solid states and aqueous *** compared with the solution state,the fluorescence enhancement of SF-RC and SF-β are,respectively,7 times and 17 times in the(CdTe QDs@SF/LDH)_(n)UTFs,indicating that the LDH nanosheets favor the fluorescence enhancement effect on the CdTe QDs@*** fabricated materials displayed fluorescence response to a biological molecule such as immune globulin,***,the(CdTe QDs@SF/LDH)_(n)UTFs has a potential to be used as biosensor.

关键词： CdTe quantum dots Silk fibroin Layered doubled hydroxide Co-assembly Fluorescence enhancement

Alleviation of Copper-induced Seed Germination Inhibition and Oxidative Damage in Alfalfa by Methane-rich Water

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Alleviation of Copper-induced Seed Germination Inhibition an...

作者： muhammad kaleem Samma 南京农业大学

学位级别：硕士

Background and aims:Recently,researchers discovered the protective effects of methane（CH4）in animals against oxidative ***,its possible physiological roles in plants are largely ***,we evaluate the role of copper（Cu）-stressed increase in CH4 production from germinating alfalfa ***:We adopted physiological,histochemical and biochemical approaches to study the beneficial role of CH4 in the alleviation of Cu toxicity in germinating alfalfa ***:Our results showed that endogenous production of CH4 was significantly increased in copper（Cu）-stressed alfalfa seeds as compared to ***,results showed that the pretreatment with 30%saturation of MRW significantly alleviated the inhibition of seed germination and seedling growth,and oxidative stress induced by Cu stress,the latter of which were supported by the decrease of TBARS content and reduction of Cu-induced loss of plasma membrane integrity in MRW-pretreated alfalfa ***,MRW could increase a/p amylase activities and sugar contents,both of which were consistent with the alleviation of seed *** accumulation was also obviously decreased by *** comparison with Cu stress alone,pretreatment with 30%saturation of MRW significantly decreased the Cu-induced stimulation of POD and P5CS activities,and as well proline accumulation,While,the inhibition of activities of ascorbate peroxidase（APX）,superoxide dismutase（SOD）,catalase（CAT）and corresponding transcripts（APX1/2,PDH,Cu/Zn SOD and Mn-SOD）,were ***:Together,our results provide a new evidence for the potential beneficial role of MRW in the alleviation of Cu toxicity during alfalfa seed germination.

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