Molecular and in vitro biochemical assessment of chemosensory protein 10 from brown planthopper Nilaparvata lugens at acidic pH

作     者：Muhammad Irfan WARIS Aneela YOUNAS Rana Muhammad Kaleem ULLAH Fatima RASOOL Muhammad Muzammal ADEEL WANG Man-qun Muhammad Irfan WARIS;Aneela YOUNAS;Rana Muhammad Kaleem ULLAH;Fatima RASOOL;Muhammad Muzammal ADEEL;WANG Man-qun

作者机构：College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan 430070P.R.China National Center for BioinformaticsQuaid-i-Azam UniversityIslamaba 45320Pakistan College of InformaticsHuazhong Agricultural UniversityWuhan 430070P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2022年第21卷第3期

页      面：781-796页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：supported and funded by the National Key Research and Development Program of China(2017YFE0113900) the Special Technical Innovation of Hubei Province,China(2017ABA146) 

主　　题：insect olfaction chemosensory protein Nilaparvata lugens fluorescence competitive binding assay fluorescence quenching assay molecular docking 

摘      要：Chemosensory proteins(CSPs)are important molecular components of the insect olfactory system,which are involved in capturing,binding,and transporting hydrophobic odour molecules across the sensillum in sensillar lymph in regulating insect *** protein family(CSPs)is also involved in many other systems that are not linked to olfactory receptors in olfactory *** brown planthopper(BPH)is a monophagous pest of rice that causes damage by sucking phloem sap and transmitting a number of diseases caused by *** this study,fluorescence competitive binding assay and fluorescence quenching assay at acidic p H were performed as well as homology modelling to describe the binding affinity of Nlug *** competitive binding assay(FCBA)demonstrated that Nlug CSP10 bound strongly to nonadecane,farnesene,and 2-tridecanone at acidic p *** results of FCBA indicated that Nlug CSP10 bound different ligands at the physiological p H(5.0)of the bulk sensillum *** quenching assay demonstrated that Nlug CSP10 generated a stable complex with 2-tridecanone,while two ligands nonadecane and farnesene collided due to molecular *** interaction of selected ligands with the modelled structure of Nlug CSP10 was also analyzed,which found the key amino acids(Gln23,Gln24,Gln25,Asn27,Met33,Ser34,Ile35,Tyr36,Asn42,Met43,Val45,Asn46,Asn93,Arg96,Ala97,Lys99,and Ala100)in Nlug CSP10 that were involved in binding of volatile *** present study contributes to the binding profile of Nlug CSP10 that promotes the development of behaviorally active ligands based on BPH olfactory system.