Genome-wide identification and spatiotemporal expression profiling of zinc finger SWIM domain-containing protein family genes

作     者：Imtiaz Ul Hassan Hafiz Mamoon Rehman Ziran Liu Liangji Zhou Muhammad Kaleem Samma Chengdong Wang Zixin Rong Xufeng Qi Dongqing Cai Hui Zhao Imtiaz UI Hassan;Hafiz Mamoon Rehman;Ziran Liu;Liangji Zhou;Muhammad Kaleem Samma;Chengdong Wang;Zixin Rong;Xufeng Qi;Dongqing Cai;Hui Zhao

作者机构：Key Laboratory for Regenerative MedicineMinistry of EducationSchool of Biomedical SciencesFaculty of MedicineThe Chinese University of Hong KongHong Kong SARChina Centre for Agricultural Biochemistry and Biotechnology(CABB)University of Agriculture FaisalabadFaisalabad 38000Pakistan Qingdao Municipal Center for Disease Control and PreventionQingdaoShandong 266034China Key Laboratory of Regenerative Medicine of Ministry of EducationDepartment of Developmental&Regenerative BiologyJinan UniversityGuangzhou 511436China Department of Biology and ChemistryCity University of Hong KongHong Kong SARChina Department of Gene TechnologySchool of Engineering Sciences in ChemistryBiotechnology and Health(CBH)KTH Royal Institute of Technology10691 StockholmSweden Kunming Institute of Zoology-The Chinese University of Hong Kong(KIZ-CUHK)Joint Laboratory of Bioresources and Molecular Research of Common DiseasesHong Kong SARChina Hong Kong Branch of CAS Center for Excellence in Animal Evolution and GeneticsThe Chinese University of Hong KongHong Kong SARChina 

出 版 物：《Zoological Research》 (动物学研究（英文）)

年 卷 期：2023年第44卷第3期

页      面：663-674页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0711[理学-系统科学] 07[理学] 08[工学] 

基　　金：supported by the National Key R&D Program of China,Synthetic Biology Research(2019YFA0904500) Research Grants Council of Hong Kong(14119120,14112618,and CRF C5033-19E to H.Z.) Additional support was provided by the Hong Kong Branch of CAS Center for Excellence in Animal Evolution and Genetics,Chinese University of Hong Kong 

主　　题：ZSWIM gene family Xenopus Gene expression Phylogenetic analysis Bioinformatics 

摘      要：The biological function of the novel zinc-finger SWIM domain-containing protein family(ZSWIM)during embryonic development remains ***,we conducted a genome-wide analysis to explore the evolutionary processes of the ZSWIM gene family members in mice,Xenopus tropicalis,zebrafish,and *** identified nine putative ZSWIM genes in the human and mouse genome,eight in the Xenopus genome,and five in the zebrafish *** on multiple sequence alignment,three members,ZSWIM5,ZSWIM6,and ZSWIM8,demonstrated the highest homology across all four *** available RNA sequencing(RNAseq)data,ZSWIM genes were found to be widely expressed across different tissues,with distinct tissuespecific *** identify the functions of the ZSWIM protein family during embryogenesis,we examined temporal and spatial expression patterns of zswim family genes in Xenopus *** real-time polymerase chain reaction(qRT-PCR)revealed that each member had a distinct expression ***-mount in situ hybridization showed that both zswim1 and zswim3 were maternally expressed genes;zswim5 and zswim6were expressed throughout embryogenesis and displayed dynamic expression in the brain,eyes,somite,and bronchial arch at the late tailbud stages;zswim7 was detected in the eye area;zswim8 showed a dynamic expression pattern during the tailbud stages,with expression detected in the brain,eyes,and somite;zswim9 was faintly expressed throughout embryonic *** study provides a foundation for future research to delineate the functions of ZSWIM gene members.