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Correlation of gut microorganisms and non-volatile flavor substances provides new insights for breeding Scylla paramamosain

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Journal of Oceanology and Limnology 2024年第4期42卷 1336-1347页

作者： Gao GAO Xing LI Kangxiang QIN Yun HU Xiaosong JIANG Chenxi CHE Yuntao LI changkao mu Chunlin WANG Huan WANG School of Marine Science Ningbo UniversityNingbo 315211China Key Laboratory of Applied Marine Biotechnology Ministry of EducationNingbo UniversityNingbo 315211China

The farming of Scylla paramamosain with specific flavors has a higher commercial value,and the flavors are related to the integrated farming environment and non-volatile flavor substances,while the survival environment is one of the important ways to source gut microorganisms in the *** this study,the levels of dominant taxa in the gut flora of *** from Mong Cai,Vietnam(VN),Taishan City,Guangdong Province(TS)of China,and Ninghai County,Ningbo City(NB)Zhejiang Province of China converged with those of *** from Sanmen County,Ningbo City(CK 1,CK 2,and CK 3)at 28 d of *** top 15 genera with the highest abundance of VN,TS,and NB gut flora were the same as CK 1,CK 2,and CK 3,but with different percentages,and gradually converged to CK 1,CK 2,and CK 3,respectively,at 28 d of *** between intestinal flora and non-volatile flavor substances in the hepatopancreas at the percentage level of relative abundance of bacterial genera found that above 28 d of domestication,muribaculaceae,Psychrilyobacter,Clostridia_vadinBB 60_group,Halarcobacter Carboxylicivirga,Sediminispirochaeta may be the most important genera affecting flavor amino acids of ***,Carboxylicivirga,Halarcobacter,Photobacterium,ZOR 0006,Psychrilyobacter,and Pseudomonas may be the most important genera affecting flavor amino acids of ***,Carboxylicivirga,Halarcobacter,Photobacterium,ZOR 0006,Vibrio,and Sphingomonas may be the most important genera affecting flavor amino acids of *** results show that the intestinal flora structure of crabs from different areas were domesticated in the same area for at least 28 d before they converged to that of the domesticated crab,and the most important genera affecting the flavor amino acids of TS,VN,and NB were also *** results of this study provide a reference and basis for the technique of directional cultivation of the flavor quality of the crab.

关键词： survival environment Scylla paramamosain intestinal flora structure non-volatile flavor substance directional breeding

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三疣梭子蟹受精卵离体孵化技术

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水产学报 2024年第1期48卷 103-111页

作者：牛雪莹任志明吴佳颖母昌考王春琳宁波大学海洋学院水产生物技术教育部重点实验室宁波大学机械工程与力学学院

为了探究三疣梭子蟹受精卵的离体孵化技术及效果，本研究先后开展了受精卵块最适分离液种类及作用条件的筛选、分离液处理不同发育期受精卵离体孵化的差异、分离液处理对受精卵卵膜的结构影响，及分离液处理受精卵对孵化后幼体活力的影... 详细信息

为了探究三疣梭子蟹受精卵的离体孵化技术及效果，本研究先后开展了受精卵块最适分离液种类及作用条件的筛选、分离液处理不同发育期受精卵离体孵化的差异、分离液处理对受精卵卵膜的结构影响，及分离液处理受精卵对孵化后幼体活力的影响实验。结果显示，木瓜蛋白酶是一种较为理想的分离液，在浓度为0.09 g/mL，分离时间30 min时，分离率可达到99%以上；经过分离液处理后的各期受精卵均能孵化出幼体，卵内溞状幼体期离体胚胎孵化率最高，为89.0%±3.3%，未经处理的对照组为70.0%±4.8%；卵裂期离体胚胎孵化率最低，为58.0%±3.9%，对照组为31.0%±2.3%，与对照组相比，处理组的孵化率明显提高。透射电镜结果显示，经过分离液处理的受精卵卵膜结构疏松，且厚度降低，符合处理组孵化率增加这一现象，干露、福尔马林溶液胁迫和行为学测试对不同处理组的幼体进行质量评价的结果显示，处理组和对照组幼体活力无显著差异。研究表明，实验所获得的分离液可以有效提高三疣梭子蟹受精卵的分离率和孵化率，且不影响幼体质量，可为三疣梭子蟹及其他甲壳动物受精卵的离体孵化提供参考。本研究可为三疣梭子蟹的苗种繁育提供新的技术手段，也将为基因编辑辅助育种等技术的实施奠定基础。

关键词：三疣梭子蟹离体孵化孵化率幼体活力

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Postmortem Metabolite Profile Changes of mud Crab(Scylla paramamosain)Under Different Storage Conditions

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Journal of Ocean University of China 2021年第3期20卷 608-618页

作者： ZHANG Limin GUO Liping mu changkao YE Yangfang WANG Chunlin School of Marine Sciences Ningbo UniversityNingbo 315211China

mud crab(Scylla paramamosain)is one of the most consumed crabs in ***,it is believed that dead mud crabs are not ***,we investigated the postmortem metabolite profile changes of the muscle and hepatopancreas of mud crab associated with storage method using nuclear magnetic resonance-based metabolomics coupled with multivariate data *** results showed that postmortem changes of trimethylamine,organic acids(lactate,formate,and succinate),as well as ATP and its breakdown products are closely affected by the storage *** metabolites can be used as indicators for monitoring loss of crab ***,dead mud crabs preserved an edible quality at least for 4 h at room temperature,12h on ice,and 16 d at−20℃,strongly suggesting that dead crab consumption quality should be determined by *** shouldn’t be discarded without considering the effects of different storage conditions on their freshness.

关键词： mud crab freshness metabolite profiling nuclear magnetic resonance

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Phospholipid Compositions in Portunus trituberculatus Larvae at Different Developmental Stages

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Journal of Ocean University of China 2022年第1期21卷 152-162页

作者： LU Zhibin SHI Ce LIU Lei mu changkao YE Yangfang WANG Chunlin Key Laboratory of Applied Marine Biotechnology Ningbo UniversityChinese Ministry of EducationNingbo 315211China Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture Ningbo 315211China

Phospholipids are used to improve the growth and survival of Portunus trituberculatus,a widely cultured crab species in ***,only total phospholipids or several classes are applied in crab *** this study,we employed a targeted lipidomic method to investigate the comprehensive phospholipid composition in *** larvae and reveal the changing phospholipid profile over the larval *** showed that *** larvae contain 112 phospholipid species belonging to 10 phospholipid classes,in which phosphatidylcholine(PC)and phosphatidylethanolamine(PE)species are the most abundant,and PC,PE,phosphatidic acid,and phosphatidylserine(PS)are with high *** levels of all phospholipids significantly changed with larval development,which was highlighted by the downward parabolic changes in PE,phosphatidylglycerol,phosphatidylino-sitol,PS,lysophosphatidic acid,and sphingomyelin *** addition,nearly all phospholipid species were depleted at the M stage,which probably contributed to the mass mortality of crab *** findings on the composition and alterations of phospholipids in *** larvae provide novel perspectives for the targeted supplementation of phospholipids in crab *** work also highlights the use of targeted UHPLC-MS lipidomics in understanding the changes of phospholipids during crab development.

关键词： Portunus trituberculatus larval development phospholipid UHPLC-MS

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music stimulus has a positive eff ect on survival and development of the larvae in swimming crab Portunus trituberculatus

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Journal of Oceanology and Limnology 2022年第3期40卷 1277-1285页

作者： Zhiming REN Jiechi WANG Chunlin WANG changkao mu Yangfang YE Ce SHI Key Laboratory of Applied Marine Biotechnology Ningbo UniversityChinese Ministry of EducationNingbo 315211China Collaborative Innovation Center for Zhejiang Marine High-effi ciency and Healthy Aquaculture Ningbo 315211China

The sound stimulus has a signifi cant impact on marine ***,many studies have focused on the negative impacts of sound on marine *** positive impacts of sound on marine animals,especially on marine invertebrates,were rarely *** the present study,we evaluated the eff ects of music on the survival and development of an economically important marine invertebrate,swimming crab Portunus *** pieces of classical music and a control group,i.e.,Eine Kleine Nachtmusik by Mozart(EK),Bach-Violin Concerto No.1(BV),Anonymous Romanza by Anonymous(AR),and environmental sound(EN)were *** sound pressure received were 163-dB root mean square(RMS)re 1μPa for EK,166-dB RMS re 1μPa for BV,165-dB RMS re 1μPa for AR,44-dB RMS re 1μPa for EN,*** larvae were subject to 3-h classical music transmission every day during the entire larval development *** result showed that BV and AR signifi cantly elevated the cumulative survival rate of the larva from 3.42%±1.77%(EN)to 10.65%±1.69%(BV)and 7.12%±1.37%(AR)respectively(Pmusic did not aff ect the cumulative development time of larvae from ZoeaⅠto ZoeaⅣ,it signifi cantly increased the molting rate of megalopa from 24.00%±11.78%(EN)to 44.00%±13.06%(AR)and 46.67%±10.50%(BV)(Pmusical exposure during critical growth intervals may contribute to the survival and development of larval *** an application point of view,music intervention is much economically *** observed music eff ects imply that music could further enhance growth,welfare,and production.

关键词： swimming crab Portunus trituberculatus sound classical music oxygen consumption

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Dynamic metabolite alterations of Portunus trituberculatus during larval development

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Journal of Oceanology and Limnology 2019年第1期37卷 361-372页

作者： SHI Ce ZENG Tinglan LI Ronghua WANG Chunlin YE Yangfang mu changkao Key Laboratory of Applied Marine Biotechnology Ningbo University Chinese Ministry of Education Collaborative Innovation Center for Zhejiang Marine High-effi ciency and Healthy Aquaculture

A mass mortality often occurs from molting to the megalopa stage during the larval development of the swimming crab Portunus trituberculatus. Larvae with insuffcient nutrient accumulation during the zoeal stages are probably unable to develop into juvenile swimming crabs. However, the nutritional information such as the primary metabolites is scarce for P. trituberculatus larvae. The aim of this work is to obtain an insight into the metabolite traits of P. trituberculatus at early developmental stages. 1 H nuclear magnetic resonance spectroscopy coupled with multivariate data analysis was used to determine how the metabolite pro?les shift during larval development in P. trituberculatus. Our results show that the trend of total metabolites exhibited a rise from zoea 1 to zoea 3, followed by a drop from zoea 4 to megalopa and recovery during the ?rst juvenile stage. A large-scale depletion of total metabolites in the zoea 4 and megalopa stages suggests a deep depression of metabolic activity, which may be linked to the mass mortality from molting to the megalopa stage. These ?ndings provided essential metabolic information about the larval development of P. trituberculatus and important clues for understanding the nutritional requirements of P. trituberculatus in early developmental stages.

关键词： Portunus trituberculatus larval development metabolite phenotype nuclear magnetic resonance(NMR)

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Effect of Long-Term Low-Salinity Culture on the Survival, Growth, and Nutrient Composition of mud Crab Scylla paramamosain

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Journal of Ocean University of China 2022年第1期21卷 179-185页

作者： JI Zilu LI Ronghua WANG Chunlin ZHANG Zhouyi ZHANG Weijia mu changkao SONG Weiwei Key Laboratory of Applied Marine Biotechnology Ministry of EducationNingbo UniversityNingbo 315211China Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture Ningbo UniversityNingbo 315211China

This study investigated the effects of long-term low-salinity on the growth performance,proximate composition,amino acid and fatty acid profiles of Scylla *** salinity of the low-salinity-culture group(LC)and maintained-salinity-cul-ture group(MC)were set at 5 and 25 *** rearing for 30 d,the survival rate(SR)of the LC group was significantly lower,whereas the average daily molting frequency(ADMF)was significantly higher than those in MC group(Pmuscle of *** total amino acids(TAA),essen-tial amino acids(EAA)and essential amino acid index(EAAI)had no significant difference between the two *** crabs from LC group had a significantly higher HUFA,including EPA,DHA,*** percentage of n-3 PUFA and the ratio of n-3/n-6 PUFA were significantly higher in the LC *** results indicated that *** rearing at low salinity had significantly de-creased SR;however,the low-salinity environment significantly promoted the accumulation of unsaturated fatty acids(UFAs),espe-cially the n-3 PUFA,which might be related to the osmoregulation mechanism.

关键词： Scylla paramamosain low salinity survival growth nutrient composition

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Community composition,co-occurrence,and environmental drivers of bacterioplankton community in surface and 50-m water layers in the subarctic North Pacific

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Journal of Oceanology and Limnology 2023年第6期41卷 2309-2323页

作者： Quandong XIN Jufa CHEN changkao mu Xinliang WANG Wenjing LIU Tao JIANG Yan LI College of Life Sciences Yantai UniversityYantai 264005China Key Laboratory of Sustainable Development of Marine Fisheries Ministry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China School of Marine Sciences Ningbo UniversityNingbo 315211China School of Medicine Yunnan UniversityKunming 650091China

The Western Subarctic Gyre(WSG)is one of the two gyre-systems in the subarctic North Pacific known for high nutrient and low-chlorophyll ***,the bacterioplankton in marine water of this area,either in terms of the taxonomic composition or functional structure,remains relatively unexplored.A total of 22 sampling sites from two water layers(surface water,SW and 50-m layer water,FW)were collected in this *** physiochemical parameters of waters,Synechococcus,and bacterial density,as well as the bacterioplankton community composition and distribution pattern,were *** nutrient concentrations of DIN,DIP,and DSi,Chl-a concentration,and the average abundance of heterobacteria in FW were higher than those in ***,temperature and the average abundance of Synechococcus and pico-eukaryotes were higher in SW.A total of 3269 OTUs were assigned,and 2123OTUs were commonly shared;moreover,similar alpha diversity patterns were observed in both SW and *** bacterioplankton community showed significantly obvious correlation with salinity,DIP,DIN,and Chl a in both SW and ***,Cyanobacteria,Bacteroidota,Actinobacteriota,and Firmicutes were the main phyla while Synechococcus_CC9902,Psychrobacter,and Sulfitobacter were the dominant genera in each sampling *** correlations that happened between the OTUs in the cooccurrence network were positive and *** edges and graph density were found in SW,indicating that more correlations occurred,and the community was more complex in *** study provided novel knowledge on the bacterioplankton community structure and the correlation characteristics in WSG.

关键词： Western Subarctic Gyre(WSG) marine water bacterioplankton community co-occurrence network

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Disruption of bacterial balance in the gut of Portunus trituberculatus induced by Vibrio alginolyticus infection

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Journal of Oceanology and Limnology 2018年第5期36卷 1891-1898页

作者： XIA Mengjie PEI Feng mu changkao YE Yangfang WANG Chunlin Key Laboratory of Applied Marine Biotechnology（Ningbo University） Ministry of EducationNingbo 315211China Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture Ningbo 315211China

A bstract Gut microbiota impacts the health of crustaceans. V ibrio alginolyticus is a main causative pathogen that induces the vibriosis in farmed swimming crabs, Portunus trituberculatus. However, it remains unknown whether gut bacteria perform functions during the progression of vibriosis. In this study, 16 SrRNA gene amplicon sequencing was used to investigate temporal alteration of gut bacterial community in swimming crabs in response to 72-h V. alginolyticus challenge. Our results show that V. alginolyticus infection resulted in dynamic changes of bacterial community composition in swimming crabs. Such changes were highlighted by the overwhelming overabundance of V ibrio and a significant fluctuation in the gut bacteria including the bacteria with high relative abundance and especially those with low relative abundance. These findings reveal that crab vibriosis gradually develops with the infection time of V. alginolyticus and tightly relates to the dysbiosis of gut bacterial community structure. This work contributes to our appreciation of the importance of the balance of gut bacterial community structure in maintaining the health of crustaceans.

关键词： Portunus trituberculatus Vibrio alginolyticus gut bacterial community composition 16S rRNA gene amplicon sequencing

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Analysis of DNA Methylation Level of Portunus trituberculatus Subjected to Low Salinity with Methylation-Sensitive Amplification Polymorphism

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Journal of Ocean University of China 2019年第5期18卷 1158-1162页

作者： LU Shaokun LI Ronghua GAO Tianlong WANG Chunlin ZHENG Weibing mu changkao SONG Weiwei Key Laboratory of Applied Marine Biotechnology of Ministry of Education Ningbo UniversityNingbo 315211China Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture Ningbo UniversityNingbo 315211China Third Institute of Oceanography State Oceanic AdministrationXiamen 361001China

In this study,the methylation-sensitive amplification polymorphism(MSAP)was used to compare the genomic DNA methylation level of muscle,gill and hepatopancreas of Portunus trituberculatus subjected to salinity 12 for 30 days to illustrate the epigenetic mechanism of *** primers were used to analyze the difference of methylation level of different *** results showed that the baseline methylation level of muscle,hepatopancreas and gill was 47.31%,22.94%and 17.69%,*** exposed to low salinity stress,the methylation epiloci changed in the three *** demethylation and methylation processes occurred under low salinity *** methylation ratio decreased in muscle and gill but increased in *** results indicated that DNA methylation is tissue-specific when *** responds to low salinity.

关键词： Portunus trituberculatus DNA methylation methylation-sensitive amplification polymorphism low salinity gill muscle hepatopancreas

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