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A Di-D-Fructose Dianhydride-Enriched Caramel Modulates Pig Fecal Microbiota Composition

Advances in Microbiology 2014年第5期4卷 242-251页

作者： Luis A. Rubio mar.a Jesús Peinado Ana Echávarri Raquel ruiz Elena Suárez-Pereira Carmen Ortiz Mellet José M. García Fernández Departamento de Química Orgánica Facultad de Química Universidad de Sevilla Sevilla Spain Fisiología y Bioquímica de la Nutrición Animal (EEZ CSIC) Granada Spain Instituto de Investigaciones Químicas (IIQ) CSIC—Universidad de Sevilla Sevilla Spain

A correlation has been previously described between bifidobacteria counts before and after the use of a dietary additive in human studies. However, to our knowledge no information on this topic has yet been reported in animals, and no information exists either on similar possible correlations of bacterial groups other than bifidobacteria. The potential prebiotic effects of di-D-fructose dianhydride (DFA)-enriched caramels have been previously reported in laboratory animals, poultry and pigs. In the present work, twelve growing male castrated pigs (41.8 ± 1.9 kg mean BW) were fed in succession on a control (no additive) or DFA-enriched caramel (20 g/kg) containing diets. Another group of 10 pigs (38.0 ± 3.7 kg mean BW) fed on a control diet without any additive was used as negative control. Bacterial log10 number of copies of the 16S rRNA gene was determined in fecal samples by using qPCR. Increased (P10 number of copies were determined in fecal samples of pigs fed on the caramel containing diet compared with non-caramel controls. In addition, for all bacterial groups studied microbiological values co-variated with initial counts and, except for enterobacteria, variations in the fecal bacterial numbers after caramel supplementation correlated (P< 0.05) with the fecal numbers before supplementation. In conclusion, the supplementation of pig diets with DFA-enriched caramels induced significant increases in the fecal number of copies of bacterial groups regarded as beneficial, and variations in the fecal number of copies correlated with the initial fecal number of copies.

关键词： Di-D-Fructose Dianhydrides Fecal Microbiota Correlations Pig Prebiotics

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Associated or Concomitant Diseases Influence Significantly the Health-Sickness Concept in Celiacs

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Open Journal of Epidemiology 2017年第2期7卷 139-158页

作者： Eduardo Cueto Rúa mar.a Teresita Gonzalez Villar Ricardo Wright Claudia Losada Gómez Karol martínez mar.a Arregui Luciana Guzmán Cecilia Zubiri Viviana Bernedo Anabella Zosi Lorena Menéndez Lucas ruiz Leopoldo Mancinelli mar.a Urrutia Ricardo Drut School of Medical Sciences La Plata National University La Plata Argentina

It is well-known that feeling to be a healthy or sick person most probably results from the mind than from the body. We all know healthy people who feel sick and vice versa. We were interested in the health and sickness feeling of celiac people, their autorating of these feelings and its conditioning factors as well as their expectations. In this paper we present the results of an inquiry to evaluate these situations. We performed a descriptive, transversal and prospective study for 2 years to groups of celiacs and their families. They received a closed inquiry to be completed before the beginning of the talk. The inquiry included personal data and the co-existence of associated or concomitant (AoC) diseases. Most of the sample’s patients felt to be a healthy person (86.8%). Mothers see their children as healthy and the auto rated criteria is significantly better than the adult celiac person (“t”= -6.024 (p = 0.000)). AoC diseases influenced negatively in the feeling of being healthy and strongly decreased the autorating. Longer time passed on treatment reflects an increase feeling of health and of the autorating. In people with AoC diseases and who feel sick, the increased time of treatment did not show significant differences. People with “gluten sensitivity” felt sicker and auto rated themselves with a lower number than celiacs. Many pediatric gastroenterologists notice that the newly agreed definition of celiac disease, referring it as “autoimmune, chronic, incurable, and multisystemic”, results in a very negative character of the condition which might compromise the future labour of this people as well as their admittance to different health insurance systems.

关键词： Associated Conditions Celiac Disease Feeling Gluten-Free Diet Gluten Intolerance Quality of Life Health/Sickness Pain Self-Qualification

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Assessment of the exploitable biomass of thread herring(Opisthonema spp.)in northwestern Mexico

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Acta Oceanologica Sinica 2021年第9期40卷 53-65页

作者： marcelino ruiz-Dominguez Casimiro Quinonez-Velazquez Dana Isela Arizmendi-rodriguez Victor Manuel Gomez-Munoz Manuel Otilio Nevarez-martinez Facultad de Ciencias del mar Universidad Autonoma de SinaloaMazatlan 82000Mexico Instituto Politecnico Nacional Centro Interdisciplinario de Ciencias MarinasLa Paz 23000Mexico Instituto Nacional de Pesca y Acuacultura Centro Regional de Investigacion Pesquera Unidad GuaymasGuaymas 85400Mexico

In recent years,the small pelagic fishery on the Pacific northwest coast of Mexico has significantly increased fishing pressure on thread herring Opisthonema *** fishery is regulated using a precautionary approach(acceptable biological catch(ABC)and minimum catch size).However,due to fishing dynamics,fish aggregation habits and increased fishing mortality,periodic biomass assessments are necessary to estimate ABC and assess the resource *** Catch-MSY approach was used to analyze historical series of thread herring catches off the western Baja California Sur(BCS,1981–2018)and the Gulf of California(GC,1972–2018)to estimate exploitable biomass and target reference points in order to obtain catch *** to the results,in GC,the maximum biomass reached in 1972(at the beginning of fishery)and minimum biomass reached in 2015;the estimated exploitable biomass for 2019 was 42.2×10^(4) t;and the maximum sustainable yield(MSY)was 15.4×10^(4) *** the western BCS coast,the maximum biomass was reached in 1981(at the beginning of fishery)and minimum biomass was reached in 2017;the estimated exploitable biomass for 2019 was 3.2×10^(4) t;and the MSY was 1.2×10^(4) *** stocks showed a decrease in biomass over the past years and were currently near to point of full *** results suggest that the use of the Catch-MSY method is suitable to obtain annual biomass estimates,in order to establish an ABC,to know the current state of the resource,and to avoid overcoming the potential recovery of the stocks.

关键词： Catch-MSY thread herring exploitable biomass estimate

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An inter-laboratory study of DNA-based identity,parentage and species testing in animal forensic genetics

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Forensic Sciences Research 2022年第4期7卷 708-713页

作者： Sreetharan Kanthaswamy Torsten Brendel Luis Cancela Denise A.Andrade de Oliveira Bertram Brenig Carmen Cons Julian A.Crespi markéta Dajbychová Andreas Feldl Tomohito Itoh Vincenzo Landi Amparo martinez Malgorzata Natonek-Wisniewska Robert F.Oldt Anna Radko Oscar Ramírez Clementina Rodellar Manuel ruiz-Girón David Schikorski mar.a Elena Turba Guillermo Giovambatista School of Mathematics and Natural Sciences Arizona State University(ASU)at the West CampusGlendaleAZUSA California National Primate Research Center University of CaliforniaDavisCAUSA Evolutionary Biology Graduate Program School of Life SciencesArizona State UniversityTempeAZUSA Eurofins Genomics Europe Applied Genomics GmbH EbersbergGermany IDENTITAS MontevideoUruguay Laboratório de Genética Escola de Veterinária da UFMGBelo HorizonteBrazil Institute of Veterinary Medicine University of GoettingenGoettingenGermany Facultad de Veterinaria Laboratorio de Genética Bioquímica(LAGENBIO)Instituto Agroalimentario de Aragón-IA2(Universidad de Zaragoza-CITA)ZaragozaSpain Facultad de Ciencias Veterinarias UNLP IGEVET-Instituto de Genética Veterinaria(UNLP-CONICET LAPLATA)La PlataArgentina Genomia s.r.o. PlzeňCzech Republic Maebashi Institute of Animal Science Livestock Improvement Association of Japan(LIAJ)MaebashiJapan Department of Veterinary Medicine Animal Breeding and GeneticsUniversity of Bari“Aldo Moro”ValenzanoItaly Department of Genetics Faculty of Veterinary SciencesUniversity of CórdobaCórdobaSpain Department of Animal Molecular Biology National Research Institute of Animal ProductionBalicePoland Vetgenomics SL BarcelonaSpain Laboratorio de Biología Molecular y Genómica Hispalis Biolab S.L.U.SevilleSpain Laboratoire LABOFARM-GENINDEXE LOUDEACCedexFrance Genefast SRL ForlìItaly

The probative value of animal forensic genetic evidence relies on laboratory accuracy and ***-laboratory comparisons allow laboratories to evaluate their performance on specific tests and analyses and to continue to monitor their *** International Society for Animal Genetics(ISAG)administered animal forensic comparison tests(AFCTs)in 2016 and 2018 to assess the limitations and capabilities of laboratories offering forensic identification,parentage and species determination *** AFCTs revealed that analyses of low DNA template concentrations(≤300 pg/μL)constitute a significant challenge that has prevented many laboratories from reporting correct identification and parentage ***,a lack of familiarity with species testing protocols,interpretation guidelines and representative databases prevented over a quarter of the participating laboratories from submitting correct species determination *** laboratories showed improvement in their genotyping accuracy over ***,the use of forensically validated standards,such as a standard forensic short tandem repeat(STR)kit,preferably with an allelic ladder,and stricter guidelines for STR typing,may have prevented some common issues from occurring,such as genotyping inaccuracies,missing data,elevated stutter products and loading *** AFCTs underscore the importance of conducting routine forensic comparison tests to allow laboratories to compare results from each *** should keep improving their scientific and technical capabilities and continuously evaluate their personnel’s proficiency in critical techniques such as low copy number(LCN)analysis and species *** this is the first time that the ISAG has conducted comparison tests for forensic testing,findings from these AFCTs may serve as the foundation for continuous improvements of the overall quality of animal forensic genetic testing.

关键词： Forensic sciences forensic genetics comparison test forensic DNA analysis individual identification parentage test species test

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Determining the specific status of the Iberian sturgeons by means genetic analyses of old specimens

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Advances in Bioscience and Biotechnology 2010年第3期1卷 171-179页

作者： Francisca Robles Belén Cano-Roldán Carmelo ruiz Rejón Luís Javier martínez-González mar.a Jesús álvarez-Cubero José Antonio Lorente José Antonio Riquelme Cantal Pedro Aguayo de Hoyos Javier Carrasco Rus Miguel Cortés Sánchez mar.a Dolores Simón Vallejo Manuel ruiz Rejón Roberto de la Herrán 不详

To clarify the species status of sturgeon from rivers of the Iberian Peninsula, eight molecular markers (4 nuclear and 4 mitochondrial) have been analysed in different specimens from historical museum samples and prehistoric samples from archaeological sites. These analyses indicate that one of these specimens (UGP captured in the Guadalquivir River in the 19th century) is A. sturio, based on all the eight molecular markers, four of them used from the first time in this study. In previous analyses based on 5 genetic markers, our group assigned two specimens captured in this river in the 1970-80s (EBD8173 and EBD8401) to the species A. naccarii, suggesting the presence of this species in the Iberian Peninsula. In this work, this conclusion is drawn after successfully obtaining a mitochondrial marker in a very old scute from a prehistoric site (Acinipo, about 1500 BC, from the Guadalquivir River basin). On the other hand, in the specimen EBD8174 captured in the Guadalquivir in 1975, we have obtained two new mitochondrial markers confirming that it can be considered A. sturio for all the mitochondrial markers, but nuclear ones identify it as A. naccarii. Finally, two very old samples (Nerja E-VI and Nerja N/62-63) were not successfully characterized by any molecular markers. Some aspects and consequences of our results are discussed, such as the origin of the “mosaic” specimen EBD8174 and, above all, the native status of A. naccarii in historic and prehistoric times in the southern Iberian Peninsula.

关键词： Iberian Sturgeons A. naccarii A. sturio Ancient DNA Genetic Identification Molecular markers.

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Molecular Assessment of Athecate Dinoflagellates of the Order Gymnodiniales (Dinophyceae) in Todos Santos Bay, Baja California México

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American Journal of Plant Sciences 2021年第12期12卷 1926-1944页

作者： Alexis De Jesús Escarcega-Bata mary Carmen ruiz-de la Torre mar.a Luisa Nuñez Resendiz Luis Manuel Enriquez-Paredes Kurt M. Dreckmann Abel Sentíes marine Science Faculty Autonomous University of Baja California Ensenada México Department of Hydrobiology Universidad Autónoma Metropolitana-Iztapalapa México México

The Gymnodiniales are the most important group of athecate dinoflagellates both for its abundance and distribution and for the harmful potential of several of its species. Although morphologically it is well known, phylogenetically it has been very little studied. HABs impact important coastal activities in Todos Santos Bay, so a detailed characterization of potentially toxic phytoplankton species is crucial in monitoring surveys. Therefore, the aim of the present study was to carry out a molecular assessment to the morphospecies of Gymnodiniales currently recorded in Todos Santos Bay, from which discussed their phylogenetic relationships. 120 samples were obtained from monthly samplings during 2019 to 2020. For all of them, 18 s small subunit (SSU) and 28 s large subunit (LSU) genes of rDNA were amplified and sequenced using single-cell PCR. Also, they were photographed. A phylogenetic analysis was performed on MrBayes from the combined 18 s - 28 s data set. The presence of 15 phylogenetic entities was confirmed, four of which constituted new records for the Mexican Pacific, as well as three harmful species, one toxic and two bloom-forming. Our molecular results evidenced the need to re-evaluate the current taxonomic system of athecate dinoflagellates at all hierarchical levels, integrating them with morphological evidence without preponderance of this criterion over taxonomic decisions.

关键词： Diversity HABS New Records Paraphyletic Groups Athecate Dinoflagellates

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Synthesis of Silver Nanoparticles Using Albumin as a Reducing Agent

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Materials Sciences and Applications 2011年第6期2卷 578-581页

作者： J.E Morales-Sánchez jesus Guajardo-Pacheco mar.a-Eugenia Noriega Quintero Cristina Morales Elpidio Compeán-Jasso J González-Hernández ruiz Facundo 不详

The preparation of stable, uniformed silver nanoparticles by the reduction of silver ions using albumin is reported in the present paper. It is a simple process for obtaining silver nanoparticles. The samples have been characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM), which reveal the nature of the nanoparticles. These studies conclude that the particles are mostly spherical in shape and have an average size of 26 nm. The UV-vis spectra show that an absorption band, occurred because of the Surface Plasmon Resonance, existing at 443 nm;the Thermogravimetric Analysis (TGA) shows that only 2% of the weight is organic matter. The average particle size was measured with a Nanosizer DLS.

关键词： Reducing Agent Albumin Silver Nanoparticles

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HIV-Infected Adolescent: A Case Report

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World Journal of AIDS 2011年第4期1卷 165-168页

作者： Apezteguia Fernández Carolina Hernández Muniesa Belén Vicente Sánchez mar.a del Pilar álvarez García Ana ruiz Jiménez mar.a Ramos Amador José Tomás 不详

We report the case of a Nigerian adolescent recently arrived to Spain, who presented at the emergency room with severe respiratory distress. She had been previously diagnosed of HIV-1 discontinuing antiretroviral therapy, what was hidden by the family. This case illustrates the difficulties in management and stigma in HIV-infected adolescents, particularly immigrants and the need to collect all the information available before starting antiretroviral therapy.

关键词： HIV Adolescent Immigrant

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Historical fluxes of metal and metalloids in an aquatic ecosystem affected by land-use change and mining activities in northwestern Mexico

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International Journal of Sediment Research 2023年第5期38卷 724-738页

作者： Ochoa-Contreras Roberto Jara-marini martín Enrique ruiz-Fernández Ana Carolina Sanchez-Cabeza Joan Albert Meza-Figueroa Diana Pérez-Bernal Libia Hascibe Posgrado en Ciencias Centro de Investigación en Alimentación y Desarrollo Unidad Hermosillo 2. Centro de Investigación en Alimentación y Desarrollo Unidad Hermosillo Unidad Acad emica Mazatlán Instituto de Ciencias del Mar y Limnología Universidad Nacional Autónoma de M exico Departamento de Geología División de Ciencias Exactas y Naturales Universidad de Sonora Rosales y Encinas

Freshwater reservoirs are essential owing because of their ecological, economic, and social *** are particularly vulnerable to contamination, as of metal and metalloids, derived from anthropogenic activities like mining. The temporal variations in trace element concentrations(arsenic(As),mercury(Hg), chromium(Cr), copper(Cu), nickel(Ni), lead(Pb), and zinc(Zn)), enrichment, fluxes, and possible sources were evaluated by studying two sediment cores from the La Angostura(ANG) Reservoir(northwest Mexico), using210Pb geochronology. The enrichment factors showed from null to minor enrichment for most elements, but moderate to severe enrichment of mercury(Hg). Most trace element concentrations had a detrital origin, and notable Hg concentration increases since the past decade were associated with severe drought periods, likely resulting from wildfires. The observed sediment concentrations of As and Hg can cause adverse effects on biota in the ecosystem since they are above the probable effect level(PEL). Development of strategies for metal attenuation in this reservoir is recommended and metals should be controlled until specific ecotoxicological studies are performed.

关键词： Aquatic ecosystems ~(210)Pb geochronology Mining activities Metals Metalloids Enrichment factors

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Recent advances and new insights in the management of early-stage epidermal growth factor receptor-mutated non-small-cell lung cancer

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World Journal of Clinical Oncology 2021年第10期12卷 912-925页

作者： Miguel J Sotelo Jose Luis García Cesar Torres-Mattos Héctor Milián Carlos Carracedo mar.a Angeles González-ruiz Xabier Mielgo-Rubio Juan Carlos Trujillo-Reyes Felipe Couñago Department of Medical Oncology Hospital María AuxiliadoraDepartment of Medical OncologyCentro Oncológico AliadaOncological Research UnitClínica San GabrielLima 15801Peru Department of Thoracic Surgery Hospital Universitario La PrincesaDepartment of Thoracic SurgeryMD Anderson Cancer CenterDepartment of Thoracic SurgeryHospital HMMadrid 28006Spain Department of Medical Oncology Hospital Nacional Guillermo AlmenaraOncological Research UnitClínica San GabrielLima 15033Peru Department of Thoracic Surgery Hospital Universitario La PrincesaMadrid 28006Spain Department of Medical Oncology Centro Oncológico AliadaLima 15036Peru Department of Radiation Oncology Hospital Universitario Virgen MacarenaSevilla 41009Spain Department of Oncology Hospital Universitario Fundación AlcorcónAlcorcón 28922MadridSpain Department of Thoracic Surgery Hospital de la Santa Creu i Sant PauBarcelona 08041Spain Department of Radiation Oncology Hospital Universitario Quirónsalud MadridHospital La LuzUniversidad Europea de MadridMadrid 28223Spain

Patients with early-stage non-small-cell lung cancer(NSCLC)are candidates for curative surgery;however,despite multiple advances in lung cancer management,recurrence rates remain *** chemotherapy has been demonstrated to significantly prolong overall survival(OS),but this benefit is modest and there is an urgent need for effective new therapies to provide a cure for more *** high efficacy of tyrosine kinase inhibitors(TKIs)against epidermal growth factor receptor-mutated(EGFR)in patients with advanced EGFR-mutated NSCLC has led to the evaluation of these agents in early stages of the *** clinical trials have evaluated the safety and efficacy of EGFR TKIs as an adjuvant treatment,in patients with resected EGFR-mutated NSCLC,and shown that they significantly prolong disease-free survival(DFS),but this benefit does not translate to ***,an interim analysis of the ADAURA trial demonstrated that,surprisingly,osimertinib improved *** led to the study being stopped early,leaving many unanswered questions about its potential effect on OS and its incorporation as a standard adjuvant treatment in this patient *** targeted agents are also being evaluated in locally-advanced disease,with promising results,although prospective studies with larger sample sizes are needed to confirm these *** this article,we review the most relevant studies on the role of EGFR TKIs in the management of early-stage EGFR-mutated NSCLC.

关键词： Non-small-cell lung cancer Early stage Epidermal growth factor receptormutated Epidermal growth factor receptor-mutated-tyrosine kinase inhibitor Adjuvant Neoadjuvant

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