An inter-laboratory study of DNA-based identity,parentage and species testing in animal forensic genetics
作者机构：School of Mathematics and Natural SciencesArizona State University(ASU)at the West CampusGlendaleAZUSA California National Primate Research CenterUniversity of CaliforniaDavisCAUSA Evolutionary Biology Graduate ProgramSchool of Life SciencesArizona State UniversityTempeAZUSA Eurofins Genomics Europe Applied Genomics GmbHEbersbergGermany IDENTITASMontevideoUruguay Laboratório de GenéticaEscola de Veterinária da UFMGBelo HorizonteBrazil Institute of Veterinary MedicineUniversity of GoettingenGoettingenGermany Facultad de VeterinariaLaboratorio de Genética Bioquímica(LAGENBIO)Instituto Agroalimentario de Aragón-IA2(Universidad de Zaragoza-CITA)ZaragozaSpain Facultad de Ciencias Veterinarias UNLPIGEVET-Instituto de Genética Veterinaria(UNLP-CONICET LAPLATA)La PlataArgentina Genomia s.r.o.PlzeňCzech Republic Maebashi Institute of Animal ScienceLivestock Improvement Association of Japan(LIAJ)MaebashiJapan Department of Veterinary MedicineAnimal Breeding and GeneticsUniversity of Bari“Aldo Moro”ValenzanoItaly Department of GeneticsFaculty of Veterinary SciencesUniversity of CórdobaCórdobaSpain Department of Animal Molecular BiologyNational Research Institute of Animal ProductionBalicePoland Vetgenomics SLBarcelonaSpain Laboratorio de Biología Molecular y GenómicaHispalis Biolab S.L.U.SevilleSpain Laboratoire LABOFARM-GENINDEXELOUDEACCedexFrance Genefast SRLForlìItaly
出 版 物：《Forensic Sciences Research》 (法庭科学研究（英文）)
年 卷 期：2022年第7卷第4期
页 面：708-713页
核心收录：
学科分类：0402[教育学-心理学(可授教育学、理学学位）] 0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0303[法学-社会学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 0703[理学-化学] 071002[理学-动物学] 0702[理学-物理学]
主 题：Forensic sciences forensic genetics comparison test forensic DNA analysis individual identification parentage test species test
摘 要：The probative value of animal forensic genetic evidence relies on laboratory accuracy and ***-laboratory comparisons allow laboratories to evaluate their performance on specific tests and analyses and to continue to monitor their *** International Society for Animal Genetics(ISAG)administered animal forensic comparison tests(AFCTs)in 2016 and 2018 to assess the limitations and capabilities of laboratories offering forensic identification,parentage and species determination *** AFCTs revealed that analyses of low DNA template concentrations(≤300 pg/μL)constitute a significant challenge that has prevented many laboratories from reporting correct identification and parentage ***,a lack of familiarity with species testing protocols,interpretation guidelines and representative databases prevented over a quarter of the participating laboratories from submitting correct species determination *** laboratories showed improvement in their genotyping accuracy over ***,the use of forensically validated standards,such as a standard forensic short tandem repeat(STR)kit,preferably with an allelic ladder,and stricter guidelines for STR typing,may have prevented some common issues from occurring,such as genotyping inaccuracies,missing data,elevated stutter products and loading *** AFCTs underscore the importance of conducting routine forensic comparison tests to allow laboratories to compare results from each *** should keep improving their scientific and technical capabilities and continuously evaluate their personnel’s proficiency in critical techniques such as low copy number(LCN)analysis and species *** this is the first time that the ISAG has conducted comparison tests for forensic testing,findings from these AFCTs may serve as the foundation for continuous improvements of the overall quality of animal forensic genetic testing.
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