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metabolic dysfunction-associated steatotic liver disease-associated fibrosis and cardiac dysfunction in patients with type 2 diabetes

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World Journal of Cardiology 2024年第10期16卷 580-594页

作者： Simona Cernea Danusia Onișor Andrada Larisa Roiban Theodora Benedek Nora rat Department m3/Internal medicine I George Emil Palade University of MedicinePharmacyScience and Technology of Târgu MureșTârgu Mureş540142Romania Diabetes Nutrition and Metabolic Diseases Outpatient UnitEmergency County Clinical HospitalTârgu Mureş540136Romania Department mE2/Internal medicine VII George Emil Palade University of MedicinePharmacyScience and Technology of Târgu MureşTârgu Mureş540142Romania Gastroenterology Clinic MureșCounty Clinical HospitalTârgu Mureş540103Romania Diabetes Compartment MediașMunicipal HospitalMediaș551030Romania Doctoral School of medicine and Pharmacy George Emil Palade University of MedicinePharmacyScienceand Technology of Târgu MureşTârgu Mureş540142Romania Department m3/Internal medicine VI George Emil Palade University of MedicinePharmacyScienceand Technology of Târgu MureşTârgu Mureş540142Romania Department of Cardiology Emergency County Clinical HospitalTârgu Mureş540136Romania

BACKGROUND metabolic dysfunction-associated steatotic liver disease(mASLD),particularly in the presence of liver fibrosis,increases the risk of cardiovascular morbidity and mortality,but the nature of the cardio-hepatic interaction in the context type 2 diabetes mellitus(T2Dm)is not fully *** To evaluate the changes in cardiac morphology and function in patients with T2Dm and mASLD-associated liver *** T2Dm patients with mASLD underwent a medical evaluation that included an assessment of lifestyle,anthropometric measurements,vital signs,an extensive laboratory panel,and a standard *** fibrosis was evaluated using two scores[Fibrosis-4(FIB4)and Non-alcoholic fatty liver disease-Fibrosis Score(NFS)],and subjects were classified as having advanced fibrosis,no fibrosis,or an indeterminate *** correlations between structural and functional cardiac parameters and markers of liver fibrosis were evaluated through bivariate and multiple regression *** significance was set at Pm 267 T2Dm-mASLD subjects with complete assessment was *** with scores indicating advanced fibrosis exhibited higher interventricular septum and left ventricular(LV)posterior wall thickness,atrial diameters,LV end-systolic volume,LV mass index(LVmi),and epicardial adipose tissue thickness(EATT).Their mean ejection fraction(EF)was significantly lower(49.19%±5.62%vs 50.87%±5.14%vs 52.00%±3.25%;P=0.003),and a smaller proportion had an EF≥50%(49.40%vs 68.90%vs 84.21%;P=0.0017).Their total and mid LV wall motion score indexes were higher(Pmarkers of diastolic dysfunction,with a higher E/e’ratio[9.64±4.10 vs 8.44(2.43-26.33)vs 7.35±2.62;P=0.026],and over 70%had lateral e’valuesm/second,though without significant differences between *** multiple regression analyses,FIB4 correlated with left atrium diameter(LAD;β=0.044;P<0.05),and NFS with both LAD(β=0.039;P<0.05)and right

关键词： metabolic dysfunction-associated steatotic liver disease Type 2 diabetes mellitus Liver fibrosis Cardiac dysfunction Sex-hormone binding protein

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Experimental investigation on combustion and emission characteristics of reactivity controlled compression ignition engine powered with iso-propanol/biodiesel blends

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Propulsion and Power Research 2022年第2期11卷 224-239页

作者： müjdat Fırat S¸ehmus Altun mutlu Okcu Yasin Varola Department of Automotive Engineering Fırat UniversityElazıg23119Turkey Department of mechanical Engineering Batman UniversityBatman72100Turkey Department of Electrical-Electronic Engineering Ardahan UniversityArdahan75002Turkey

Recently, reactivity controlled compression ignition (RCCI) has been proposed inorder to achieve a higher thermal efficiency with lower emissions than conventional combustion. In RCCI mode, as the fuel types and their combinations affects the reactivity stratificationinside cylinder, thus combustion control, in present study, iso-propanol was evaluated as lowreactivity fuel (LRF) when petroleum diesel, commercial biodiesel and their blends were highreactivity fuels. It is of great importance that iso-propanol and biodiesel be used together inRCCI mode, as they significantly affect the in-cylinder stratification due to their high octane/cetane number. Therefore, the reactivity controlled compression ignition (RCCI) combustioncharacteristics was investigated in a diesel research engine using iso-propanol, petroleumdiesel, biodiesel and their blends as fuels. Tests were conducted on varying loadings (from20% to 60% of max torque) and premixed ratios of LRF (Rp Z 0, 0.15, 0.30, 0.45, and0.60) at a constant engine speed of 2400 rpm. Results, which were compared with conventionaldiesel combustion (CDC), showed that, as the premixed ratio (Rp) of low-reactivity fuel (isopropanol) increased, ignition delay (ID) period prolonged while combustion duration (CD) and rate of pressure rise (RoPR) reduced assisted to reduce NO emissions and smoke opacity in theexhaust. NO and smoke opacity reduced simultaneously for biodiesel-propanol combinationsup to 40% under 20% load and 0.60 premixed ratio of LRF compared to CDC. Propanol premixed ratio of 0.30 at 60% load was found to be optimum concerning lowest emissions. In conventional mode, HC emissions reduced by up to 52% when biodiesel and its blends with dieselfuel are used, whereas they increased significantly in RCCI mode. According to overall results,it is concluded that RCCI performed better than CDC at entire load.

关键词： Reactivity controlled compression ignition(RCCI) Propanol Biodiesel Combustion Emissions

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In vivo and in vitro study of resorbable magnesium wires for medical implants:mg purity,surface quality,Zn alloying and polymer coating

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Journal of magnesium and Alloys 2024年第6期12卷 2472-2488页

作者： K.Tesar J.Luňácková m.Jex m.Žaloudková R.Vrbová m.Bartoš P.Klein L.Vištejnová J.Dušková E.Filová Z.Sucharda m.Steinerová S.Habr K.Balík A.Singh Department of materials Faculty of Nuclear Sciences and Physical EngineeringCzech Technical University in PragueTrojanova 13Prague 12000Czech Republic Institute of Physics Czech Academy of SciencesNa Slovance 2Prague 18221Czech Republic Institute of Dental medicine First Faculty of MedicineCharles University and General University Hospital in PragueKaterinská32Prague 12801Czech Republic Department of Physics Faculty of Nuclear Sciences and Physical EngineeringCzech Technical University in PragueBrehová7Prague 11519Czech Republic Department of Composites and Carbon materials Institute of Rock Structure and MechanicsCzech Academy of SciencesV Holešovickách 41Prague 18209Czech Republic Institute of Anatomy First Faculty of MedicineCharles UniversityU Nemocnice 3Prague 12800Czech Republic Biomedical Center Faculty of Medicine in PilsenCharles UniversityAlej Svobody 76Pilsen 32300Czech Republic Department of Pathological Physiology Faculty of Medicine in PilsenCharles UniversityAlej Svobody 7632300 PilsenCzech Republic Department of Histology and Embryology Faculty of Medicine in PilsenCharles UniversityAlej Svobody 7632300 PilsenCzech Republic Institute of Pathology First Faculty of MedicineCharles University and General University Hospital in PragueStudnickova 2Prague 12800Czech Republic Department of Biomaterials and Tissue Engineering Institute of PhysiologyCzech Academy of SciencesVidenska 1083Prague 14200Czech Republic Research Center for Structural materials National Institute for Materials Science1-2-1 SengenTsukuba 305-0047Japan

magnesium is an excellent material in terms of biocompatibility and its corrosion products can serve as an active source for new bone ***,localized corrosion and H_(2)generation limit the potential of mg-based *** low-alloyed mg-Zn wires can strongly reduce problems with large H_(2)bubbles and improve the mechanical properties considerably while maintaining excellent long-term *** pickling and a polymer coating can be effectively used to lower the rate of in vivo *** this work,microstructural,mechanical,and in vitro characterization of 250μm and 300μm extruded wires made from ultra-pure mg,commercially pure mg,mg-0.15Zn,mg-0.4Zn and mg-1Zn was ***,mg-0.4Zn wires together with a variant coated with a copolymer of L-lactide andε-caprolactone were tested in vivo on artificially damaged Wistar rat *** on the observed mg-induced osteogenesis,polymer-coated mg wires with a small addition of Zn are a perspective material for bone-support applications,such as cerclage and fixation wires.

关键词： magnesium Resorbable mg wire mg-Zn implant degradation Biocompatibility study Zn grain boundary segregation

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Neuroprotective effect of liraglutide and memantine in a rat model of Alzheimer’s disease

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Asian Pacific Journal of Tropical Biomedicine 2024年第5期14卷 207-214页

作者： Duaa m.Bakhshwin Foziya W.Alhalabi mohamad Omar Barasheed Ahmed m.Bakhshwin maha H.Jamal mohammed A.Bazuhair Fatemah O.Kamel Rania m.magadmi Zoheir A Damanhouri Samar m.Alsaggaf Department of Clinical Pharmacology Faculty of MedicineKing Abdulaziz UniversityJeddahSaudi Arabia Hera General Hospital MakkahSaudi Arabia Department of Pathology Faculty of MedicineKing Abdulaziz UniversityJeddahSaudi Arabia Department of Clinical Anatomy Faculty of MedicineKing Abdulaziz UniversityJeddahSaudi Arabia

Objective:To assess the effect of memantine combined with liraglutide on aluminum chloride(AlCl_(3))and D-galactose(D-GAL)-induced neurotoxicity in ***:male Wistar rats were divided into 5 groups of 5 animals each:the positive control,the negative control,the memantine-treated group,the liraglutide-treated group,and the combination group treated with memantine and ***_(3)and D-GAL were used to induce *** tests,brain beta-amyloid protein,and oxidative stress biomarkers were ***:The morris water maze test indicated an enhanced memory in the combination ***,the combination treatment of liraglutide and memantine resulted in a remarkable reduction in the beta-amyloid protein level in the brain *** inflammation and oxidative stress biomarkers were significantly reduced,and the levels of antioxidant parameters were ***:The combination of liraglutide and memantine exerts neuroprotective effects and enhances memory and cognitive functions in rats with Alzheimer’s disease.

关键词： Alzheimer’s disease Cognitive function Glucagonlike peptide-1 Liraglutide memantine

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Benefits of Ilizarov automated bone distraction for nerves and articular cartilage in experimental leg lengthening

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World Journal of Orthopedics 2017年第9期8卷 688-696页

作者： Nathalia Shchudlo Tatyana Varsegova Tatyana Stupina michael Shchudlo m.rat saifutdinov Andrey Yemanov Laboratory of morphology FSBI Russian Ilizarov Scientific Center "Restorative Traumatology and Orthopaedics"

AIm To determine peculiarities of tissue responses to manual and automated Ilizarov bone distraction in nerves and articular *** Twenty-nine dogs were divided in two experimental groups: Group m-leg lengthening with manual distraction(1 mm/d in 4 steps), Group A-automated distraction(1 mm/d in 60 steps) and intact group. Animals were euthanized at the end of distraction, at 30 th day of fixation in apparatus and 30 d after the fixator removal. m-responses in gastrocnemius and tibialis anterior muscles were recorded, numerical histology of peronealand tibialis nerves and knee cartilage semi-thin sections, scanning electron microscopy and X-ray electron probe microanalysis were *** Better restoration of m-response amplitudes in leg muscles was noted in A-group. Fibrosis of epineurium with adipocytes loss in peroneal nerve, subperineurial edema and fibrosis of endoneurium in some fascicles of both nerves were noted only in m-group, shares of nerve fibers with atrophic and degenerative changes were bigger in m-group than in A-group. At the end of experiment morphometric parameters of nerve fibers in peroneal nerve were comparable with intact nerve only in A-group. Quantitative parameters of articular cartilage(thickness, volumetric densities of chondrocytes, percentages of isogenic clusters and empty cellular lacunas, contents of sulfur and calcium) were badly changed in m-group and less changed in *** Automated Ilizarov distraction is more safe method of orthopedic leg lengthening than manual distraction in points of nervous fibers survival and articular cartilage arthrotic changes.

关键词： Limb lengthening Articular cartilage Nerve Histomorphometry Dog

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紫外光直接光氧化浸出铜渣

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Journal of Central South University 2024年第5期31卷 1476-1493页

作者： Zeynel Abidin SARI m.Deniz TURAN Department of metallurgy-Iskenderun Vocational School of Higher Education Iskenderun Technical University31200HatayTurkey Department of metallurgy and materials Engineering Fırat University23119ElazığTurkey

采用光化学反应研究了铜渣中Cu和Fe的溶解行为。实验采用石英玻璃夹套,在玻璃反应釜中间歇进行,在反应釜下埋入紫外线灯,并采用玻璃管作为送风装置。研究了不同波长UVA(365 nm)、UVB(311 nm)、UVC(254 nm)和真空UV光(185 nm)在浸出液中... 详细信息

采用光化学反应研究了铜渣中Cu和Fe的溶解行为。实验采用石英玻璃夹套,在玻璃反应釜中间歇进行,在反应釜下埋入紫外线灯,并采用玻璃管作为送风装置。研究了不同波长UVA(365 nm)、UVB(311 nm)、UVC(254 nm)和真空UV光(185 nm)在浸出液中的行为。所有实验在相同条件下,在有和没有紫外线灯的情况下进行比较。研究了自由基形成机制对浸出环境的适应性及其在氧化溶液介质浸出中的可用性。在紫外光(185 nm)和非紫外光条件下,铜的提取率分别为85.1%和70.7%。综上所述,在紫外(185 nm)光反应器体系中,铜渣在最佳条件下的金属溶解率比无紫外光反应器体系效率更高。光化学反应器是使其适应湿法冶金应用和检验工艺的新途径。

关键词：浸出铜炉渣光化学氧化紫外线灯氧化自由基

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A rat model of multicompartmental traumatic injury and hemorrhagic shock induces bone marrow dysfunction and profound anemia

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Animal models and Experimental medicine 2024年第3期7卷 367-376页

作者： Lauren S.Kelly Jennifer A.munley Erick E.Pons Kolenkode B.Kannan Elizabeth m.Whitley Letitia E.Bible Philip A.Efron Alicia m.mohr Department of Surgery and Sepsis and Critical Illness Research Center University of Florida College of MedicineGainesvilleFloridaUSA Pathogenesis LLCGainesvilleFloridaUSA

Background:Severe trauma is associated with systemic inflammation and organ *** rodent trauma models are the mainstay of postinjury research but have been criticized for not fully replicating severe human *** aim of this study was to create a rat model of multicompartmental injury which recreates profound traumatic ***:male Sprague-Dawley rats were subjected to unilateral lung contusion and hemorrhagic shock(LCHS),multicompartmental polytrauma(PT)(unilateral lung contusion,hemorrhagic shock,cecectomy,bifemoral pseudofracture),or na?ve ***,plasma toll-l ike receptor 4(TLR4),hemoglobin,spleen to body weight ratio,bone marrow(Bm)erythroid progenitor(CFU-GEmm,BFU-E,and CFU-E)growth,plasma granulocyte colony-stimulating factor(G-CSF)and right lung histologic injury were assessed on day 7,with significance defined as p valuesma resulted in markedly more profound inhibition of weight gain compared to LCHS(p=0.0002)along with elevated plasma TLR4(pmoglobin(pratios(p=0.004).Both LCHS and PT demonstrated suppression of CFU-E and BFU-E growth compared to naive(pma G-CSF was elevated in PT compared to both na?ve and LCHS(pmonstrated significant histologic right lung injury with poor alveolar wall integrity and interstitial ***:multicompartmental injury as described here establishes a reproducible model of multicompartmental injury with worsened anemia,splenic tissue enlargement,weight loss,and increased inflammatory activity compared to a less severe *** may serve as a more effective model to recreate profound traumatic injury to replicate the human inflammatory response postinjury.

关键词： anemia i nflammation polytrauma pseudofracture shock

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Effects of stem cells applications on oxidative stress and apoptosis during implantation

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Asian pacific Journal of Reproduction 2018年第5期7卷 229-235页

作者： I■■l Aydemir mahmud m.ozkut Fatma F■rat Alican Gümürüdü Dila Hatun Sal Kübra Erdogan Asl■Goker mehmet■brahim Tuglu Department of Histology and Embryology Faculty of MedicineNi■de■mer Halisdemir University51240 Ni■deTurkey Department of Histology and Embryology Faculty of MedicineManisa Celal Bayar University45030 ManisaTurkey Izmir International Biomedicine and Genome Institute(IBG-Izmir) Dokuz Eylül University35340 IzmirTurkey Department of Neuroscience Health Science InstituteDokuz Eylül University35340 IzmirTurkey Department of Anatomy Faculty of MedicineIzmir Katip■elebi University35620 IzmirTurkey Department of Obstetrics and Gynecology Faculty of MedicineManisa Celal Bayar University45030 ManisaTurkey

Objective:To investigate the effects of bone marrow derived mesenchymal stem cell (BmSC) application into the rat endometrium on oxidative stress, cell proliferation and ***: The female rats selected in estrous cycle were divided into three groups (saline, media and BmSC group). The intrauterine and intraperitoneal injections were performed using the saline (200 μL), culture media (200 μL) and 1×106 BmSCs/200 μL culture media, and then they were mated with male rats. On the 7th day of the pregnancy, uterine samples were harvested and dyed with heamatoxylin-eosin histochemically, anti-endothelial nitric oxide synthase and anti-inducible nitric oxide synthase, and anti-proliferating cell nuclear antigen immunohistochemically, with terminal deoxynucleotidyl transferas dUTP nick end labeling for apoptosis. The stainings were evaluated by H-score and the results were analyzed using one-way ANOVA test ***: It was found that BmSCs increased the endometrial thickness, endometrial epithelium thickness and number of endometrial glands compared to control and sham groups. The intrauterine BmSC application decreased both anti-endothelial nitric oxide synthase and anti-inducible nitric oxide synthase immunoreactivities and the number of apoptotic cells compared to the intraperitoneal applications whereas the immunoreactivity of proliferating cell nuclear antigen was ***: In current study, we define that stem cells do not cause any structural damages. Also they change the distribution of oxidative stress and cell proliferation marker. These findings support the reliability of stem cells in clinical use in the case of infertility.

关键词： mesenchymal stem cell Implantation Endometrium Oxidative stress Proliferation Apoptosis

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Biochemical Liver Functions and molecular Identification of Fasciola hepatica from Experimentally Infected rat model

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Open Journal of Animal Sciences 2024年第2期14卷 88-100页

作者： Omnia m. Kandil Emad B. Ata margarita P. Gabrashanska Hatem A. Shalaby Tamer H. Abd El-Aziz Noha m. F. Hassan Soad m. Nasr mohamed A. Helal Ebtesam m. Al-Olayan Department of Parasitology & Animal Diseases Veterinary Research Institute National Research Centre Giza Egypt Institute of Experimental morphology Pathology and Anthropology with Museum Bulgarian Academy of Sciences Sofia Bulgaria Department of Zoology Collage of Science King Saud University Riyadh Saudi Arabia

The current study was performed to evaluate the liver function status as well as molecular characterization of the recovered worms in rats experimentally infected with F. hepatica. Sixteen male Wister rats aged 30 days were randomly allocated into two groups (n = 8). The first group was infected orally with 15 viable encysted metacercaria of F. hepatica per animal. The other group was kept non-infected (control group). At zero time (before infection), the 2nd, 4th, 6th, 8th, 10th, 12th and 14th weeks post-infection (WPI), blood and serum samples were collected via puncture of retro-orbital plexus of veins from each rat. Serum enzyme level (AST and ALT) and total protein were measured, and the serum protein profile was carried out using agarose gel electrophoresis. During the period of the experiment, serum ALT and AST activities and serum total globulins significantly increased while serum total proteins and albumin markedly decreased in the infected group. On the 14th WPI, the data of the electropherogram showed that globulin fractions (α1-, β- and γ-globulin) levels were significantly increased while α2-globulin was markedly decreased in the infected group. The molecular analysis confirmed the amplification of the ITS1, ITS2 and NDI genes of F. hepatica recovered from the infected liver of rats with amplicon sizes of 630, 510 and 560 bp, respectively. Sequencing of the amplified ITS gene resulted in the determination of 3 strains (PP108836, PP108837, and PP108838). Also, analysis of the ITS2 gene resulted in obtaining 3 isolates under the accession numbers (PP109065, PP109066, and PP109067). In conclusion, fasciolosis in the rat model is suitable for routine experimental infections and caused a pronounced liver dysfunction with discharging of the Fasciola eggs in the faeces and the development of adult stages in the bile ducts. Furthermore, molecular techniques are a sensitive

关键词： Fasciola hepatica Liver Functions Serum Enzymes Serum Protein Electrophoresis molecular Characterization

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Investigation of the Effect of Rosmarinic Acid on Cyclophosphamide-Induced Gonadal Toxicity

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Advances in Sexual medicine 2022年第1期12卷 1-8页

作者： Fırat Şahin Fırat Aşır Ebru Gökalp Özkorkmaz Süreyya Özdemir Başaran Özge Kaplan Işılay Sezen Ermiş Engin Deveci Department of Histology and Embryology Faculty of Medicine Dicle University Diyarbakir Turkey

The aim of this study was to investigate the effect of rosmarinic acid against gonadal toxicity caused by cyclophosphamide, an important anticancer drug. A total of 28 rats were divided into 4 groups, with 7 animals in each group. The groups were created as follows;Group 1 (control) (n = 7): Subjects received only 1 ml of 0.9% saline solution per day intraperitoneally for 14 days. Group 2 (Rosmarinic Acid) (n = 7): The subjects were given 20 mg/kg Rosmarinic acid intraperitoneally for 14 days. Group 3 (Cyclophosphamide) (n = 7): Only 1 ml of 0.9% saline solution was administered intraperitoneally to the subjects for the first 7 days. Cyclophosphamide 20 mg/kg per day was administered intraperitoneally for the last 7 days (from the 8th day). Group 4 (Rosmarinic Acid + Cyclophosphamide) (n = 7): The subjects were given 20 mg/kg Rosmarinic acid daily intraperitoneally for 14 days. From the 8th day of the experiment to the end of the experiment (last 7 days of the experiment), 20 mg/kg cyclophosphamide was given intraperitoneally daily. At the end of the experiment, body weights of all rats were measured first. Afterwards, the weights of testicular tissue samples were measured and the averages of the weights were taken. Routine tissue follows up was performed on the testicular tissues taken. Hematoxylin-eosin staining was applied to tissue sections of 5 μm thickness. As a result of the statistical analysis, it was determined that cyclophosmadine decreased body and testicular weight, but rosmarinic acid had a protective effect on the contrary. It was concluded that cyclophosmadine caused damage t

关键词： Testis Cyclophosphamide Rosmarinic Acid Sertoli Cell Hematoxylin Eosin

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