Effects of stem cells applications on oxidative stress and apoptosis during implantation

作     者：I■■l Aydemir Mahmud M.ozkut Fatma F■rat Alican Gümürüdü Dila Hatun Sal Kübra Erdogan Asl■Goker Mehmet■brahim Tuglu 

作者机构：Department of Histology and EmbryologyFaculty of MedicineNi■de■mer Halisdemir University51240 Ni■deTurkey Department of Histology and EmbryologyFaculty of MedicineManisa Celal Bayar University45030 ManisaTurkey Izmir International Biomedicine and Genome Institute(IBG-Izmir)Dokuz Eylül University35340 IzmirTurkey Department of NeuroscienceHealth Science InstituteDokuz Eylül University35340 IzmirTurkey Department of AnatomyFaculty of MedicineIzmir Katip■elebi University35620 IzmirTurkey Department of Obstetrics and GynecologyFaculty of MedicineManisa Celal Bayar University45030 ManisaTurkey 

出 版 物：《Asian pacific Journal of Reproduction》 (亚太生殖杂志（英文版）)

年 卷 期：2018年第7卷第5期

页      面：229-235页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This study was financially supported by the Scientific Research Committee of Manisa Celal Bayar University  Turkey (Project number: 2013-063) 

主　　题：Mesenchymal stem cell Implantation Endometrium Oxidative stress Proliferation Apoptosis 

摘      要：Objective:To investigate the effects of bone marrow derived mesenchymal stem cell (BMSC) application into the rat endometrium on oxidative stress, cell proliferation and ***: The female rats selected in estrous cycle were divided into three groups (saline, media and BMSC group). The intrauterine and intraperitoneal injections were performed using the saline (200 μL), culture media (200 μL) and 1×106 BMSCs/200 μL culture media, and then they were mated with male rats. On the 7th day of the pregnancy, uterine samples were harvested and dyed with heamatoxylin-eosin histochemically, anti-endothelial nitric oxide synthase and anti-inducible nitric oxide synthase, and anti-proliferating cell nuclear antigen immunohistochemically, with terminal deoxynucleotidyl transferas dUTP nick end labeling for apoptosis. The stainings were evaluated by H-score and the results were analyzed using one-way ANOVA test ***: It was found that BMSCs increased the endometrial thickness, endometrial epithelium thickness and number of endometrial glands compared to control and sham groups. The intrauterine BMSC application decreased both anti-endothelial nitric oxide synthase and anti-inducible nitric oxide synthase immunoreactivities and the number of apoptotic cells compared to the intraperitoneal applications whereas the immunoreactivity of proliferating cell nuclear antigen was ***: In current study, we define that stem cells do not cause any structural damages. Also they change the distribution of oxidative stress and cell proliferation marker. These findings support the reliability of stem cells in clinical use in the case of infertility.