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锦州市三级甲等医院实习护生职业决断力在欺凌行为与职业探索间的中介效应

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职业与健康 2024年第12期40卷 1673-1677页

作者：张健吴春燕李红玉锦州医科大学护理学院山西医科大学护理学院

目的了解锦州市三级甲等医院实习护生欺凌行为、职业决断力与职业探索的相关性,探讨职业决断力在欺凌行为与职业探索间的中介效应,为护理教育者和管理者采取针对性策略,提高护生职业探索水平提供依据。方法采用便利抽样法,选取2022年6月... 详细信息

目的了解锦州市三级甲等医院实习护生欺凌行为、职业决断力与职业探索的相关性,探讨职业决断力在欺凌行为与职业探索间的中介效应,为护理教育者和管理者采取针对性策略,提高护生职业探索水平提供依据。方法采用便利抽样法,选取2022年6月—2023年3月在锦州市2所三级甲等医院实习的318名护生为研究对象,以中文版护生遭受欺凌行为量表、职业探索问卷和职业决断力问卷对实习护生进行调查,并对数据进行分析。结果三甲医院实习护生欺凌行为总分[m(P_(25),P_(75))]为16.00(10.00,22.25)分,职业决断力总分为(32.50±6.57)分,职业探索总分为(57.98±11.10)分。职业探索与职业决断力呈显著正相关(P<0.01),与欺凌行为呈显著负相关(P<0.01);Bootstrap检验结果显示,职业决断力在三甲医院实习护生欺凌行为和职业探索间的中介效应为-0.152(95%CI:-0.208~-0.099),占总效应的59.6%。结论锦州市2所三级甲等医院实习护生欺凌行为、职业决断力与职业探索间两两相关,职业决断力在三甲医院实习护生欺凌行为和职业探索间起部分负向中介作用。护理教育者和管理者应重视护生的实习环境,采取有效措施减少欺凌行为的发生,提升其职业决断力,从而促进职业探索频率的增加。

关键词：欺凌行为职业决断力职业探索中介效应实习护生三级甲等医院

On-chip detection of a single nucleotide polymorphism without polymerase amplification

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Nano Research 2014年第9期7卷 1302-1310页

作者： Jinhee han matthew Tan Lakshmana Sudheendra Robert H. Weiss Ian m. Kennedy Department of mechanical and Aerospace Engineering University of California Davis California 95616 USA Division of Nephrology Department of Internal Medicine University of California Davis California 95616 USA medical Service Sacramento VA Medical Center Sacramento California 95655 USA

A nanoparticle-assembled photonic crystal （PC） array was used to detect single nucleotide polymorphism （SNP）. The assay platform with PC nanostructure enhanced the fluorescent signal from nanoparticle-hybridized DNA complexes due to phase matching of excitation and emission. Nanoparticles coupled with probe DNA were trapped into nanowells in an array by using an electrophoretic particle entrapment system. The PC/DNA assay platform was able to identify a 1 base pair （bp） difference in synthesized nucleotide sequences that mimicked the mutation seen in a feline model of human autosomal dominant polycystic kidney disease （PKD） with a sensitivity of 0.9 fg/mL （50 am）-sensitivity, which corresponds to 30 oligos/array. The reliability of the PC/DNA assay platform to detect SNP in a real sample was demonstrated by using genomic DNA （gDNA） extracted from the urine and blood of two PKD-wild type and three PKD positive cats. The standard curves for PKD positive （PKD＋） and negative （PKD-） DNA were created using two feline-urine samples. An additional three urine samples were analyzed in a similar fashion and showed satisfactory agreement with the standard curve, confirm.ng the presence of the mutation in affected urine. The limit of detection （LOD） was 0.005 ng/mL which corresponds to 6 fg per array for gDNA in urine and blood. The PC system demonstrated the ability to detect a number of genome equivalents for the PKD SNP that was very similar to the results reported with real time polymerase chain reaction （PCR）. The favorable comparison with quantitative PCR suggests that the PC technology may find application well beyond the detection of the PKD SNP, into areas where a simple, cheap and portable nucleic acid analvsis is desirable.

关键词： photonic crystal array single nucleotidepolymorphisms DNA polycystic kidney disease real time polymerasechain reaction (PCR)

Comparative water relations of co-occurring trees in a mixed podocarp-broadleaf forest

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Journal of Plant Ecology 2019年第1期12卷 163-175页

作者： Julia Kaplick michael J.Clearwater Cate macinnis-ng School of Biological Sciences University of AucklandPrivate Bag 92019Auckland 1142New Zealand School of Science University of WaikatoPrivate Bag 3105Hamilton 3240 New Zealand

Aims As extreme climatic events including droughts and heat waves become more common in a changing climate,tree mortality has increased across the *** order to determine whether certain species have a competitive advantage over others,we explored the water-relations and leaf-gas exchange of four co-occurring spe-cies in a forest in northern Aotearoa-New *** studied the ecologically and culturally significant foundation species,Agathis australis(a conifer),two additional conifers,Phyllocladus tricho-manoides and Podocarpus totara and the angiosperm Knightia *** We measured sap flow,leaf-gas exchange and xylem water poten-tials of leaves and terminal branches with concurrent measures of micrometeorological data on days with very few *** derived whole tree hydraulic conductance and instantaneous water-use efficiency(WUEi)at our remnant forest in west Auckland during February 2015(southern hemisphere summer).Important Findings The four species behaved similarly in their diurnal curves of gas exchange and water *** of assimilation,stomatal con-ductance and WUEi were similar am.ng trees of different *** tree hydraulic conductance was also similar am.ng *** results indicate functional convergence in water relations,possibly driven by low nutrient soils at the *** results suggest that there is no species with a clear adaptive advantage over the others in the context of climate change.

关键词： southern conifers sap flow water potential hydraulic conductance leaf-gas exchange functional convergence

Incorporating geotechnical and geophysical investigations for underground obstruction detection:A case study

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Underground Space 2023年第4期11卷 116-129页

作者： Yaohui Liu Yannick C.H.ng Yunhuo Zhang Ping Yang Taeseo Ku School of mechanics and Civil Engineering China University of Mining and TechnologyXuzhou 221000China Department of Civil and Environmental Engineering National University of Singapore117576Singapore Geotechnical&Tunnel Division Land Transport Authority Singapore219428Singapore Department of Geotechnical Engineering College of Civil EngineeringTongji UniversityShanghai 200092China Department of Civil and Environmental Engineering Konkuk UniversitySeoul 05029South Korea

Determining the location and boundary of underground obstructions and/or anomalies is a common problem and often a great chal-lenge for tunneling and underground *** this study,geotechnical investigations(penetration tests and borehole drilling/sam-pling)and geophysical investigations(surface wave method and cross-hole seismic method)were conducted with the aim of identifying the location and boundary of rock obstructions in Changi East,*** surface wave method is frequently used in the sites with lateral homogeneity in previous studies,but its application in the sites with rock obstructions is *** experimental results of this study indicate that the surface wave method is also able to determine the upper surface of rock obstructions,but difficult to identify the lateral and bottom boundaries of rock *** improve the precision of detection,the full waveform inversion(FWI)method was used to process the data from the cross-hole seismic *** results indicate that the inversion precision of P-wave is higher than that of *** horizontal and vertical ranges of rock obstructions in the P-wave inversion results are 14–26 m and 7.5–11.0 m respectively,roughly consistent with the results of penetration tests(about 15–25 m)and borehole log(8.85–10.80 m).This result proves that the sequential application of first-arrival time analysis and FWI can effectively delineate the boundary of rock ***,the results of various detection methods were analyzed and compared in this *** the advantages of various methods,we propose a cost-effective and high-precision workflow containing both geotechnical and geophysical investigations.

关键词： FWI Geotechnical and geophysical investigations Cross-hole seismic method Surface wave method Obstruction detection

Control of settlement and volume loss induced by tunneling under recently reclaimed land

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Underground Space 2019年第4期4卷 289-301页

作者： A.K.L.Kwong C.C.W.ng A.Schwob AECOm Hong KongChina Dragages-Bouygues Joint Venture Hong KongChina

The proposed Tuen mun-Chek Lap Kok Link(Tm-CLKL)project comprises a 9-km-long dual two-lane carriageway between Tuen mun and North Lantau,Hong *** of the two tunnels at the Northern Landfall Fall was carried out by two TBms:the northbound is referred to as mL03 and has an excavated diameter of 17.63 m,and the southbound is referred to as mL02 and has an excavated diameter of 14.00 *** use of TBms minimized the amount of dredging and removal of soft marine deposits,resulting in less impact to the environment within and near the work site of the *** treatment in the form of deep vibro-compaction was carried out at the sand fill and band drains installed in the marine deposit.A surcharge was also added above the final formation level to limit the residual settlement and differential settlement of the reclaimed land to less than 500 mm and 1 in 300,respectively,for a design life of 50 *** element analyses were performed to predict the settlement trough under different confinement pressures along the tunnel *** monitoring of the ground movement was carried out during the driving of the two TBms to ensure that the design confinement pressure of the slurry would not cause excessive settlement,heave,or blow out at the shallow tunnel sections or different facilities along the tunnel *** monitoring data was then used to back-calculate the percentage of volume loss,which is a key index describing the behavior of the ground during *** monitoring data showed that the measured settlement and back-calculated volume loss were much lower than the predicted values.

关键词： TBm excavation Settlement trough Volume loss Ground treatment Confinement pressure

利拉鲁肽对高糖诱导的足细胞凋亡的影响及机制

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临床医药实践 2024年第8期33卷 563-568,576页

作者：荣青峰郭娜韩乐山西医科大学第二医院山西太原030001

目的:探讨利拉鲁肽对高糖诱导的足细胞凋亡的影响及机制。方法:将购买的小鼠足细胞(mPC5)分为7组:正常糖(ng)组、高糖(HG)组、甘露醇(m)组、高糖+沉默信息调节因子1(SIRT1)激动剂(HG+RSV)组、高糖+SIRT1激动剂+SIRT1阻断剂(HG+RSV+S)组... 详细信息

目的:探讨利拉鲁肽对高糖诱导的足细胞凋亡的影响及机制。方法:将购买的小鼠足细胞(mPC5)分为7组:正常糖(ng)组、高糖(HG)组、甘露醇(m)组、高糖+沉默信息调节因子1(SIRT1)激动剂(HG+RSV)组、高糖+SIRT1激动剂+SIRT1阻断剂(HG+RSV+S)组、高糖+胰高血糖素样肽-1受体(GLP-1R)激动剂利拉鲁肽(HG+LG)组,高糖+胰高血糖素样肽-1受体(GLP-1R)激动剂+GLP-1R阻断剂(HG+LG+E)组。刺激72 h后,采用流式细胞术检测足细胞凋亡率;共聚焦荧光成像技术检测足细胞骨架蛋白synaptopodin表达水平;免疫印迹检测GLP-1R,SIRT1表达。结果:HG组足细胞凋亡率升高,synaptopodin,SIRT1和GLP-1R表达降低,与ng组比较,差异有统计学意义(P<0.01);HG+RSV组足细胞凋亡率降低,SIRT1表达升高,与HG组比较,差异有统计学意义(P<0.01);而SIRT1特异性拮抗剂则阻断了SIRT1激动剂对足细胞的保护作用;HG+LG组足细胞凋亡率降低,SIRT1和GLP-1R表达升高,与HG组比较差异有统计学意义(P<0.01);而GLP-1R特异性拮抗剂则阻断了GLP激动剂对SIRTI的表达调控作用。结论:高糖可诱导足细胞发生凋亡和损伤,利拉鲁肽可减少高糖诱导的足细胞凋亡,其机制可能与激活GLP-1R/SIRT1信号途径有关。

关键词：足细胞凋亡利拉鲁肽沉默信息调节因子1

Spectral Analysis for Preconditioning of multi-Dimensional Riesz Fractional Diffusion Equations

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Numerical mathematics(Theory,methods and Applications) 2022年第3期15卷 565-591页

作者： Xin Huang Xue-Lei Lin michael K.ng Hai-Wei Sun Department of mathematics University of MacaoMacao SARChina Shenzhen JL Computational Science and Applied Research Institute Shenzhen 518131China Beijing Computational Science Research Center Beijing 100193China Department of mathematics The University of HongkongHong Kong SARChina

In this paper,we analyze the spectra of the preconditioned matrices arising from discretized multi-dimensional Riesz spatial fractional diffusion *** finite difference method is employed to approximate the multi-dimensional Riesz fractional derivatives,which generates symmetric positive definite ill-conditioned multi-level Toeplitz *** preconditioned conjugate gradient method with a preconditioner based on the sine transform is employed to solve the resulting linear ***,we prove that the spectra of the preconditioned matrices are uniformly bounded in the open interval(12,32)and thus the preconditioned conjugate gradient method converges linearly within an iteration number independent of the discretization ***,the proposed method can be extended to handle ill-conditioned multi-level Toeplitz matrices whose blocks are generated by functions with zeros of fractional *** theoretical results fill in a vacancy in the *** examples are presented to show the convergence performance of the proposed preconditioner that is better than other preconditioners.

关键词： multi-dimensional Riesz fractional derivative multi-level Toeplitz matrix sine transform based preconditioner preconditioned conjugate gradient method

An AlGaN/GaN HEmT with a reduced surface electric field and an improved breakdown voltage

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Chinese Physics B 2012年第8期21卷 360-364页

作者：谢刚 Edward Xu Niloufar Hashemi 张波 Fred Y.Fu Wai Tung ng The Edward S.Rogers Sr.Electrical and Computer Engineering Department University of Toronto State key Laboratory of Electronic Thin Films and Integrated Devices University of Electronic Science and Technology of China Crosslight Software Inc.Burnaby BCCanada

A reduced surface electric field in an AlGaN/GaN high electron mobility transistor （HEmT） is investigated by employing a localized mg-doped layer under the two-dimensional electron gas （2-DEG） channel as an electric field shaping layer. The electric field strength around the gate edge is effectively relieved and the surface electric field is distributed evenly as compared with those of HEmTs with conventional source-connected field plate and double field plate structures with the same device physical dimensions. Compared with the HEmTs with conventional sourceconnected field plates and double field plates, the HEmT with a mg-doped layer also shows that the breakdown location shifts from the surface of the gate edge to the bulk mg-doped layer edge. By optimizing both the length of mg-doped layer, Lm, and the doping concentration, a 5.5 times and 3 times the reduction in the peak electric field near the drain side gate edge is observed as compared with those of the HEmTs with source-connected field plate structure and double field plate structure, respectively. In a device with VGS = -5 V, Lm 1.5 m, a peak mg doping concentration of 8×10^17 cm-3 and a drift region length of 10 m, the breakdown voltage is observed to increase from 560 V in a conventional device without field plate structure to over 900 V without any area overhead penalty.

关键词： AlGaN/GaN HEmT reduced surface electric field mg-doped layer breakdown voltage

Development and validation of a rapid UPLC/mS method for the simultaneous determination of I3C, DIm, and related metabolites and its application to pharmacokinetics studies

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Journal of Chinese Pharmaceutical Sciences 2016年第7期25卷 477-488页

作者： Yury Gomez Hu Wang Ah-ng Tony Kong Graduate Program in Pharmaceutical Science Ernest Mario School of Pharmacy Rutgers The State University of New Jersey Department of Pharmaceutics Ernest Mario School of Pharmacy Rutgers The State University of New Jersey

Indole-3-carbinol(I3C) and diindolylmethane(DIm) are naturally derived dietary phytochemicals with promising anti-cancer properties that have been demonstrated both in vitro and in vivo. Using reversed-phase ultra-performance liquid chromatography(UPLC) coupled with mass spectrometry(mS), a rapid, specific, and high throughput method was developed and validated for the quantification and identification of I3 C, DIm, and other I3 C metabolites in plasma. Samples containing I3 C or DIm and the internal standard 4-methoxy indole(IS) were extracted using a liquid-liquid extraction technique. The mean recovery was 96.21% for I3 C and 108.5% for DIm. Separation was achieved using a Waters Acquity UPLC HSS T3, 1.8 μm, 2.1 mm×150 mm column and acetonitrile–water gradient elution. The flow rate was 0.3 m L/min and the run time was 9 min. The limits of detection and quantification for I3 C and DIm were 15 ng/m L and 25 ng/m L, respectively. Calibration curves for I3 C and DIm were linear(r2>0.99) over a concentration range of 0.025–20 μg/m L. Precision, accuracy, and stability analysis fulfilled the CDER guidelines criteria. The method was successfully applied to the determination of the pharmacokinetic parameters of I3 C or DIm after oral, intravenous, or intraperitoneal administration to Sprague Dawley rats. The method described here is superior over existing analytical methods for I3 C and its metabolites in terms of sensitivity, speed, and separation.

关键词： UPLC/mS Indole-3-Carbinol Diindolylmethane Pharmacokinetics Single-dose administration Sprague-Dawley Rats