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Environmental siblings of black agents of human chromoblastomycosis

Fungal Diversity 2014年第2期65卷 47-63页

作者： V.A.Vicente m.j.najafzadeh jiufeng Sun R.R.Gomes D.Robl S.G.marques C.m.P.S.Azevedo G.S.de Hoog CBS-KNAW Fungal Biodiversity Centre P.O.Box 851673508AD UtrechtThe Netherlands microbiology Parasitology and Pathology Post-graduation ProgramDepartment of Basic PathologyFederal University of ParanáCuritibaPRBrazil Institute for Biodiversity and Ecosystem Dynamics University of AmsterdamAmsterdamThe Netherlands Peking University Health Science Center Research Center for Medical MycologyBeijingChina Sun-Yat-sen memorial Hospital Sun Yat-sen UniversityGuangzhouChina Second medical military University ShanghaiChina King Abdulassiz University jeddah JeddahSaudi Arabia Department of Parasitology and mycology and Cancer Molecular Pathology Research CenterGhaem HospitalSchool of MedicineMashhad University of Medical SciencesMashhadIran Guangdong Provincial Institute of Public Health Guangdong Provincial Center for Disease Control and PreventionGuangzhouChina Federal University of maranhão University Hospital of the Federal University of MaranhãoSão LuísBrazil Cedro Laboratory and University Hospital of the Federal University of maranhão São LuísBrazil Institute of Biomedical Sciences University of São Paulo(USP)CEP 05508-900 São PauloBrazil

Agents of human chromoblastomycosis,a skin disease almost exclusively caused by members of the order Chaetothyriales,are assumed to be traumatically inoculated into the skin with sharp environmental materials such as plant thorns or wooden splinters carrying the respective *** the supposition that such fungi should have their main habitat in the environment,we investigated the occurrence of black fungi in living areas of patients with *** South America Fonsecaea agents are prevalent as agents of the disease,while also related Cladophialophora species,known from other types of skin infections,are known from the *** environmental isolates were preliminarily selected as possible agents of chromoblastomycosis,based on *** from ITS sequence data isolates were attributed to the genera Cladophialophora,Cyphellophora,Exophiala,Fonsecaea,Phialophora,and Veronaea.A total of 45 fungi morphologically identified as Fonsecaea or Cladophialophora isolated from debris and thorns of living prickly plants in Brazil were processed for taxonomic *** analysis revealed that the isolates indeed belonged to the Chaetothyriales,but only rarely an agent of chromoblastomycosis was concerned;only two strains of *** and one *** were isolated from debris *** remaining isolates belonged to hitherto unknown molecular siblings of *** novel taxa are introduced.

关键词： Black yeasts Environmental isolation Chromoblastomycosis Opportunistic fungi Virulence Infection route

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Cyphellophora and its relatives in Phialophora: biodiversity and possible role in human infection

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Fungal Diversity 2014年第2期65卷 17-45页

作者： Peiying Feng Qiaoyun Lu m.j.najafzadeh A.H.G.Gerrits van den Ende jiufeng Sun Ruoyu Li Liyan Xi V.A.Vicente Wei Lai Chun Lu G.S.de Hoog Third Affiliated Hospital Sun Yat-sen UniversityGuangzhouChina Centraalbureau voor Schimmelcultures KNAW Fungal Biodiversity CentreP.O.Box 851673508 AD Utrechtthe Netherlands Xiangyang Central Hospital XiangyangChina Peking University Health Science Center Research Center for Medical MycologyBeijingChina Department of Parasitology and mycology and Cancer Molecular Pathology Research CenterGhaem HospitalSchool of MedicineMashhad University of Medical SciencesMashhadIran Institute for Biodiversity and Ecosystem Dynamics University of AmsterdamAmsterdamthe Netherlands Key Laboratory of Tropical Disease Control Department of ParasitologyZhongshan School of MedicineSun Yat-sen UniversityGuangzhouChina Sun Yat-sen memorial Hospital Sun Yat-sen UniversityGuangzhouChina Department of Basic Pathology Federal University of ParanáCuritibaPRBrazil

Cyphellophora is a genus of black yeast-like fungi characterised by having simple phialides with multiseptate,curved *** from SSU and LSU data,Cyphellophora was found to be located in a well-supported clade within the Chaetothyriales comprising a number of species occurring on human skin and *** is phylogenetically close to Phialophora europaea,*** and ***,though morphologically these species produce singlecelled phialoconidia rather than multiseptate *** suttonii and *** have dark colonies and phylogenetically fit in with Cyphellophora;the type species of Pseudomicrodochium,***,has similar,septate conidia but has a hyaline *** the present study,multilocus phylogenetic analyses were combined with morphology and *** of the internal transcribed spacer region,the DNA dependent RNA polymerase II largest subunit and the partial beta tubulin gene were analysed for a set of 30 *** novel species,Cyphellophora pauciseptata and Phialophora ambigua were *** eucalypti was reduced to synonymy of *** role of the studied fungi between colonization and infection of human skin was *** virulence factors for these black yeast-like fungi were hypothesized to be the ability to assimilate monoaromatic hydrocarbons,to produce melanin pigments,and to tolerate the temperature of epidermal human skin.

关键词： Black yeasts Chaetothyriales Cyphellophora Phialophora Superficial infection Taxonomy

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A clade in the black yeasts defined by potential growth on human skin and nail

A clade in the black yeasts defined by potential growth on h...

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2012年中国菌物学会学术年会

作者： m.j.najafzadeh A.H.G Gerrits van den Ende V.A.Vicente G.S.de Hoog CBS-KNAW Fungal Biodiversity Centre Utrechtthe Netherlands Department of Parasitology and mycology Ghaem HospitalSchool of MedicineMashhad University of Medical SciencesMashhadIran Institute for Biodiversity and Ecosystem Dynamics University of AmsterdamAmsterdamthe Netherlands Department of Basic Pathology Federal University of ParanaCuritibaPRBrazil peking University Health Science Center Research Center for Medical Mycology Sun Yat-sen memorial Hospital Sun Yat-sen University

＜正＞Cyphellophora is a genus of black yeast-like fungi characterised by having simple phialides with multiseptate *** from SSU and LSU data,Cyphellophora was found to be located in a well-supported clade within the Chaetothyriales comprising a number of species occurring on human skin and nail. Cyphellophora is phylogenetically close to Phialophora europaea,*** and ***,though morphologically these species produce single-celled phialoconidia rather than multiseptate ones. Pseudomicrodochium suttonii and *** have dark colonies and phylogenetically fit in with Cyphellophora;the type species of Pseudomicrodochium,***,has similar,septate conidia but has a hyaline *** the present study,multilocus phylogenetic analyses were combined with morphology and *** of the internal transcribed spacer region,the DNA dependent RNA polymeraseⅡlargest subunit and the partial beta tubulin gene were analysed for a set of 30 *** novel species,Cyphellophora pauciseptata and Phialophora ambigua were *** eucalypti was reduced to synonymy of C. *** role of the studied fungi between colonization and infection of human skin was discussed. Putative virulence factors for these black yeast-like fungi were hypothesized to be the ability to assimilate monoaromatic hydrocarbons,to produce melanin pigments,and to tolerate the temperature of epidermal human *** identification and typing methods Amplified Fragment Length Polymorphism and Rolling Circle Amplification were used to diagnose Cyphellophora and *** vitro activities of eight antifungal drugs against 81 isolates of Cyphellophora and its relatives in Phialophora were *** black yeast-like organisms are susceptible in vitro to the newer azoles and to micafungin,but show resistance to amphotericin-B,fluconazole and terbinafin.

关键词： Cyphellophora Phialophora Taxonomy

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Chaetomium-like fungi causing opportunistic infections in humans:a possible role for extremotolerance

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Fungal Diversity 2016年第1期76卷 11-26页

作者： Sarah A.Ahmed Ziauddin Khan Xue-wei Wang Tarek A.A.moussa Hassan S.Al-Zahrani Omar A.A.lmaghrabi Deanna A.Sutton S.Ahmad johannes Z.Groenewald A.Alastruey-Izquierdo Anne van Diepeningen S.B.j.menken m.j.najafzadeh Pedro W.Crous Oliver Cornely Axel Hamprecht maria j.G.T.Vehreschild A.j.Kindo G.Sybren de Hoog Faculty of medical Laboratory Sciences University of KhartoumKhartoumSudan CBS-KNAW Fungal Biodiversity Centre P.O.Box 851673508 AD UtrechtThe Netherlands Institute for Biodiversity and Ecosystem Dynamics University of AmsterdamAmsterdamThe Netherlands Department of microbiology Faculty of MedicineKuwait UniversityKuwaitKuwait State Key Laboratory of mycology Institute of MicrobiologyChinese Academy of SciencesBeijingChina Biological Sciences Department Faculty of ScienceKing Abdulaziz UniversityJeddahSaudi Arabia Botany and microbiology Department Faculty of ScienceCairo UniversityGizaEgypt Fungus Testing Laboratory Department of PathologyUniversity of Texas Health Science Center at San AntonioSan AntonioTXUSA mycology Reference Laboratory National Centre for MicrobiologyCarlos III Institute of Health(Servicio deMicologíaCentro Nacional de MicrobiologíaInstituto de Salud Carlos III)MadridSpain Department of Parasitology and mycology Mashhad University of Medical SciencesMashhadIran German Centre for Infection Research(DZIF)at Cologne/Bonn First Department of Internal MedicineUniversity Hospital of CologneCologneGermany Institute for medical microbiology Immunology and HygieneUniversity of CologneCologneGermany Department of microbiology Sri Ramachandra UniversityPorurChennaiIndia Peking University Health Science Center Research Center for Medical MycologyBeijingChina Sun Yat-Sen memorial Hospital Sun Yat-Sen UniversityGuangzhouChina Shanghai Institute of medical mycology Changzheng HospitalSecond Military Medical UniversityShanghaiChina 17 Basic Pathology DepartmentFederal University of ParanáStateCuritibaParanáBrazil

members of the family Chaetomiaceae are ubiquitous ascosporulating fungi commonly,which reside in soil enriched with manure or cellulosic *** role as human pathogens is largely ***,the ability of some species to grow at high temperature enables them to play an important role as opportunistic *** family contains several genera and species that have never been reported to cause human ***,three new species are described;two belong to the genus Subramaniula and one represents a Chaetomium *** asteroides was isolated from various sources including eye and skin infections as well as from the natural environment,and *** was isolated from a toe *** anamorphosum was isolated from a kidney transplant patient suffering from fungal *** species described were previously misidentified as Papulaspora *** to the formation of cellular clumps or bulbil-like structures,which are characteristic of *** isolates failed to form sexual fruit bodies and ascospores remained absent,which is an unusual feature for the generally ascosporulating genera Chaetomium and Subramaniula;minute conidia from phialides were sometimes observed.

关键词： Chaetomium Desert fungi Keratitis Papulaspora Peritonitis Sterile fungi Subramaniula

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着色芽生菌病致病菌Cladophialophora carrionii体外抗真菌药敏试验研究

着色芽生菌病致病菌Cladophialophora carrionii体外抗真菌药敏试...

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2012年中国菌物学会学术年会

作者：邓淑文 G.S.de Hoog 廖万清 L.Yang m.j.najafzadeh 潘搏陈敏潘炜华 j.F.meis 上海市医学真菌研究所上海长征医院皮肤科 Institute for Biodiversity and Ecosystem Dynamics University of AmsterdamAmsterdamThe Netherlands CBS-KNAW Fungal Biodiversity Centre UtrechtThe Netherlands 北京大学医学真菌研究中心

本研究包括81株Cladophialophora carrionii菌株,由47株委内瑞拉菌株,20株中国菌株,9株南非菌株和6株澳大利亚菌株组成。检测了体外81株Cladophialophora carrionii菌株对九种抗真菌药物的体外活性。所有82株菌株的mIC90值按升序依次为:... 详细信息

本研究包括81株Cladophialophora carrionii菌株,由47株委内瑞拉菌株,20株中国菌株,9株南非菌株和6株澳大利亚菌株组成。检测了体外81株Cladophialophora carrionii菌株对九种抗真菌药物的体外活性。所有82株菌株的mIC90值按升序依次为:posaconazole和itraconazole（0.063μg/ml）,terbinafine（0.125μg/ml）,voriconazole和isavuconazole（0.25μg/ml）,micafungin（1μg/ml）,caspofungin（2μg/ml）,amphotericin B（8μg/ml）和fluconazole（64μg/ml）.

关键词：着色芽生菌病 2 Cladophialophora carrionii 3体外抗真菌药敏

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Frequency-dependent viscoelasticity effects on the wave attenuation performance of multi-layered periodic foundations

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Applied mathematics and mechanics(English Edition) 2024年第3期45卷 407-424页

作者： m.SAFI m.VAKILIFARD m.j.mAHmOODI Faculty of Civil Water&Environmental EngineeringShahid Beheshti UniversityTehran ***Iran Faculty of mechanical and Energy Engineering Shahid Beheshti UniversityTehran ***Iran

In this paper,layered periodic foundations(LPFs)are numerically examined for their responses to longitudinal and transverse modes in the time and frequency *** different unit-cells,i.e.,2-layer,4-layer,and 6-layer unit-cells,comprising concrete/rubber,concrete/rubber/steel/rubber,and concrete/rubber/steel/rubber/lead/rubber materials,respectively,are taken into ***,the viscoelasticity behavior of the rubber is modeled with two factors,i.e.,a frequency-independent(FI)loss factor and a linear frequency-dependent(FD)loss *** the extraction of the complex dispersion curves and the identification of the band gaps(BGs),the simulations of wave transmission in the time and frequency domains are performed using the COmSOL *** parametric studies evaluate the effects of the rubber viscoelasticity models on the dispersion curves and the wave transmission for the longitudinal and transverse *** results show that considering the rubber viscoelasticity enhances the wave attenuation ***,the transverse-mode damping is more sensitive to the viscoelasticity model than its longitudinal *** 6-layer unit-cell LPF exhibits the lowest BG,ranging from 4.8 Hz to 6.5 Hz.

关键词： layered metamaterial periodic foundation complex dispersion curve wave transmission diagram

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Selection of digital fabrication technique in the construction industry--A multi-criteria decision-making approach

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Frontiers of Structural and Civil Engineering 2024年第7期18卷 977-997页

作者： m.P.SALAImANImAGUDAm j.jAYAPRAKASH Department of Structural and Geotechnical Engineering School of Civil EngineeringVellore Institute of TechnologyVellore 632014India

Digital fabrication techniques,in recent decades,have provided the basis of a sustainable revolution in the construction ***,selecting the digital fabrication method in terms of manufacturability and functionality requirements is a complex *** paper presents alternatives and criteria for selection of digital fabrication techniques by adopting the multi-criteria decision-making *** alternatives considered in the study are concrete three-dimensional(3D)printing,shotcrete,smart dynamic casting,material intrusion,mesh molding,injection concrete 3D printing,and thin forming *** criteria include formwork utilization,reinforcement incorporation,geometrical complexity,material enhancement,assembly complexity,surface finish,and build *** demonstrates different multi-criteria decision-making techniques,with both subjective and objective weighting *** given ranking is based on the current condition of digital fabrication in the construction *** study reveals that in the selection of digital fabrication techniques,the criteria including reinforcement incorporation,build area,and geometrical complexity play a pivotal role,collectively accounting for nearly 70% of the overall *** the evaluated techniques,concrete 3D printing emerged as the best performer,however the shotcrete and mesh molding techniques in the second and third positions.

关键词： digital fabrication multicriteria decision-making concrete 3D printing

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Lattice Boltzmann method formulation for simulation of thermal radiation effects on non-Newtonian Al_(2)O_(3) free convection in entropy determination

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Applied mathematics and mechanics(English Edition) 2024年第6期45卷 1085-1106页

作者： m.NEmATI m.SEFID A.KARImIPOUR A.j.CHAmKHA Faculty of mechanical Engineering Yazd UniversityYazd ***Iran Department of mechanical Engineering Najafabad BranchIslamic Azad UniversityNajafabad ***Iran Faculty of Engineering Kuwait College of Science and TechnologyDoha 35004Kuwait

The simultaneous investigation on the parameters affecting the flow of electrically conductive fluids such as volumetric radiation,heat absorption,heat generation,and magnetic field(mF)is very vital due to its existence in various sectors of industry and *** present research focuses on mathematical modeling to simulate the cooling of a hot component through power-law(PL)nanofluid convection *** temperature reduction of the hot component inside a two-dimensional(2D)inclined chamber with two different cold wall shapes is *** formulation of the problem is derived with the lattice Boltzmann method(LBm)by code writing via the FORTRAN *** variables such as the radiation parameter(0–1),the Hartmann number(0–75),the heat absorption/generation coefficient(−5–5),the fluid behavioral index(0.8–1.2),the Rayleigh number(103–105),the imposed mF angle(0°–90°),the chamber inclination angle(−90°–90°),and the cavity cold wall shape(smooth and curved)are *** findings indicate that the presence of radiation increases the mean Nusselt number value for the shear-thickening,Newtonian,and shear thinning fluids by about 6.2%,4%,and 2%,*** most cases,the presence of nanoparticles improves the heat transfer(HT)rate,especially in the cases where thermal conduction dominates *** is the lowest cooling performance index and mF effect for the cavity placed at an angle of 90°.The application in the design of electronic coolers and solar collectors is one of the practical cases of this numerical research.

关键词： thermal performance analysis heat absorption/generation power-law(PL)Al_(2)O_(3)nanofluid magnetohydrodynamics natural convection volumetric radiation inclined cavity

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不规则毛霉(多变根毛霉)生物学与分子流行病学特征分析

不规则毛霉(多变根毛霉)生物学与分子流行病学特征分析

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中国菌物学会第五届会员代表大会暨2011年学术年会

作者：吕雪莲 m.j.najafzadeh 冉玉平沈永年 C.KLAASSEN 李春阳李若瑜席丽艳郝飞章强强吕桂霞胡志敏占城宋顺鹏刘维达 j.F.G.m.mEIS S.de HOOG 大连市皮肤病医院

目的研究不规则毛霉(曾用名:多变根毛霉)的生长动力学、体外药敏等生物学特征,种内基因分型特点,分析可能的流行传播途径;研究体外药物敏感性与临床转归的一致性,为临床预防及治疗不规则毛霉感染提供理论依据。方法收集临床及环境来源... 详细信息

目的研究不规则毛霉(曾用名:多变根毛霉)的生长动力学、体外药敏等生物学特征,种内基因分型特点,分析可能的流行传播途径;研究体外药物敏感性与临床转归的一致性,为临床预防及治疗不规则毛霉感染提供理论依据。方法收集临床及环境来源不规则毛霉菌株共21株,所有菌株接种于mEA培养基,绘制生长动力曲线,分析温度耐受性;应用m38-A2进行两性霉素B(AmB)、伊曲康唑(ITR)、伏立康唑(VOR)、泊沙康唑(POS)、Isavuconazole(ISA)、卡泊芬净(CAS)、米卡芬净(mICA)、Anidulfungin(ANI)及特比萘芬(TER)等8种药物的体外药敏测定。应用rDNA-LSU,ITS,RPB1和RPB2的多位点基因测序和AFLP技术进行种系发生树构建和聚类分析,比较结果的一致性并进行种内基因分型和地域分布特征分析。结果该菌的最佳生长温度为30℃,37℃时生长明显受抑,40℃时生长完全受抑。所有菌株均对AmB敏感(mIC/mIC=0.031/0.125ug/ml)其次为Posa(mIC mIC=1/2 ug/ml)少数菌株对ITR敏感(mIC/mIC=2/4 ug/ml)。VOR(mIC>16 ug/ml),ISA(mIC=16 ug/ml),CAS(mIC>8 ug/ml),mICA(mIC>8 ug/ml),ANI(mIC>8 ug/ml),TER(mIC>4 ug/ml)等药物均表现为耐药。AmB的体外结果与临床具有较好一致性,部分体外对ITR敏感的菌株,临床治疗仍失败。基于LSU,ITS,RPB1和RPB2的序列构建的种系发生树与AFLP聚类分析结果具有较好的一致性,可将多变根毛霉分为5个基因型(Clade 1-V),结合菌株地域来源、分离时间、物种来源等资料可发现,包括临床和环境来源株的CladeⅠ呈跨度较大的全球性分布,其他四个临床来源株的类群CladeⅡ-Ⅴ呈现较为集中的地域分布特点。结论该菌的感染仅以皮肤及皮下组织感染为主,与其37℃的温度耐受性差有关;结合体外药敏结果及临床转归,两性霉素B为多变根毛霉感染的一线治疗药物。多变根毛霉的传播易受地理因素影响,多限于局部区域小范围流行,非大气环流的快速传播。

关键词：不规则毛霉 AFLP 体外药敏温度耐受分子流行病学

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Consumer transparency in the production chain for plant varieties produced using new genomic techniques

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aBIOTECH 2024年第2期5卷 239-246页

作者： j.m.Lukasiewicz C.C.m.van de Wiel L.A.P.Lotz m.j.m.Smulders Wageningen University&Research P.O.Box 386NL-6700 AJ WageningenThe Netherlands

Plants edited with new genomic techniques(NGTs)currently fall under the Genetically modified Organisms Directive(2001/18/EC)in the European *** the proposal of the European Commission,NGT plants are partially exempted from the regulations of this *** proposal makes a distinction between two categories of NGT plants:NGT-1 and ***-1 category plants are considered equal to plants obtained through conventional breeding *** plants will not be labelled for the consumer,although they will be labelled as ***-2 category plants may be labelled with additional information as a positive *** of seeds of varieties made with gene editing,but not the products,would mean that most steps in the production chain are transparent,but not the last step towards ***“right to know”and increasing knowledge of gene-edited food is a common theme in food labelling towards ***,we describe current labelling regimes and registers and how these may be applied to provide transparency on gene-edited products to ***,we also look into consumer studies,which indicate a greater acceptance of gene-edited food among consumers,especially when additional benefits such as sustainability are mentioned.

关键词： Labelling New genomic techniques Consumer transparency Plant products

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