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Overexpression of phospholipase Dα gene enhances drought and salt tolerance of Populus tomentosa

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Chinese Science Bulletin 2008年第23期53卷 3656-3665页

作者： ZHANG TingTing SONG yunzhi liu YuDong GUO XingQi ZHU ChangXiang WEN FuJiang State Key Laboratory of Crop Biology Shandong Agricultural University Tai'an 271018 China

The cDNA of AtPLDα (Arabidopsis thaliana Phospholipase Dα) gene was introduced into P. tomentosa (Populus tomentosa) under the control of the Cauliflower mosaic virus 35S promoter. Southern and Northern blot analyses suggested that the AtPLDα gene has been transferred into the P. tomentosa genome. No obvious morphological or developmental difference was observed between the transgenic and wild-type (WT) plants. Drought and salt tolerance and gene expression of seedlings of several transgenic lines and WT plants (control) were studied. The results showed that the rhizogenesis rate and the average root-length of transgenic lines were significantly higher than WT plants after mannitol and NaCl treatment under the same growth conditions. Northern blot analysis indicated that the higher the PLDα expression in the transgenic plants, the more tolerant the transgenic plants are to drought and salt treatment. Meanwhile, another group of these transgenic lines and WT plants (control) were treated with PEG6000 and NaCl separately. The contents of chlorophylls and the activities of some anti-oxidant enzymes (superoxide dismutase, guaiacol peroxidase and catalase) as well as malondialde-hyde and relative electrical conductivity were analyzed. Altogether, our results demonstrated that overexpression of the PLDα gene can enhance the drought and salt tolerance in transgenic P. tomen-tosa plants.

关键词：磷脂酶转移基因组过渡表达抗氧化剂

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基于机器视觉的船舶管路滴漏监测

大连海事大学学报 2024年第1期50卷 125-133页

作者：姜兴家刘云志代英伟杜太利李顺琦邹永久张跃文孙培廷大连海事大学轮机工程学院辽宁大连116026 徐工集团工程机械股份有限公司高技术装备分公司江苏徐州221000

为在管道泄漏初期及时检测出微小的滴漏故障,提出一种管路滴漏视觉监测模型。采用均值背景差分法检测管路滴漏,提取液滴前景的特征参数,结合虚拟线圈法,基于滴漏液滴个数进行流量统计,提出滴漏体积流量的评估方案。采取搭建滴漏实验台... 详细信息

为在管道泄漏初期及时检测出微小的滴漏故障,提出一种管路滴漏视觉监测模型。采用均值背景差分法检测管路滴漏,提取液滴前景的特征参数,结合虚拟线圈法,基于滴漏液滴个数进行流量统计,提出滴漏体积流量的评估方案。采取搭建滴漏实验台的方法获取滴漏视频对模型进行验证,结果表明,该模型能够很好地检测出管道滴漏液滴,特别是对于滴漏频次较慢的泄漏,能够准确统计出滴漏个数且准确率在98%以上,泄漏体积流量的估计值相对误差在20%以内。该方法可以有效对管路滴漏进行监测,为管路维修决策的制定提供了参考。

关键词：船舶管路滴漏监测机器视觉均值背景虚拟线圈泄漏流量估计

GA BASED SYNTHESIS OF SATELLITE-BORNE MULTI-BEAM PLANAR ARRAY WITH ARBITRARY GEOMETRY

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Journal of Electronics(China) 2006年第4期23卷 637-640页

作者： Jin Jun Wang Huali Zhu Wenming liu yunzhi Institute of Communications Engineering PLA UST Nanjing 210007 China

A planar array antenna with arbitrary geometry synthesis technique based on genetic algorithm is discussed. This approach avoids coding/decoding and directly works with complex numbers to simplify computing program and to speed up computation. This approach uses two crossover operators that can over-come premature convergence and the dependence of convergence on initial population. Simulation results show that this method is capable of synthesizing complex pattern shapes of planar arrays with arbitrary ge-ometry and can realize good sidelobe suppression at the same time.

关键词： Genetic algorithm Antenna array Pattern synthesis Shaped beam antennas

A New Polynitrogen-Heterocyclic Scaffold 2,4,6,8-Tetraazanoradamantane Skeleton:Synthesis and Conversion of Its Functionalities

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Chinese Journal of Chemistry 2023年第7期41卷 783-789页

作者： ongbin Cai Bing Li Tianjiao Hou Qi Zhou yunzhi liu Huan Li Yuan Gao Longyi Zhu Jun Luo School of Chemistry and Chemical Engineering Nanjing University of Science and TechnologyNanjingJiangsu210094 China College of Chemical Engineering Nanjing Tech UniversityNanjingJiangsu211816 China

Comprehensive Summary 2,4,6,8-Tetraazanoradamantane skeleton is a unique rigid cage-like organic structure that has not been *** this article,a facile protocol to build all-bridge-position-functionalized 2,4,6,8-tetraazanoradamantane skeleton by condensation of glyoxal,primary amines and disubstituted malondialdehydes,which were in situ generated from 2,2-dipropenyl-1,3-dioxolane or 4,4-dimethoxyhepta-2,5-diene via ozonation,is reported for the first time.A variety of primary amines are compatible with this process,and the corresponding 2,4,6,8-tetraalkyl-2,4,6,8-tetraazanoradamantane-9-one ethylene or methyl ketals were achieved in 16%—58%yields.N-Benzyl group could be converted to other functionalities such as acetyl and nitro,which indicates the structural diversity of this scaffold.

关键词： Cage compounds Tetraazanoradamantane Multicomponent reactions Nitrogen heterocycles Condensation

三源重组H9N2亚型禽流感病毒NA蛋白原核表达及多克隆抗体制备

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中国病原生物学杂志 2024年第6期19卷 625-630页

作者：梁志鹏刘静刘闰栀张新宇何杰珩陈高婕闫战飞池仕红袁生郭锦玥梁昭平黄淑坚温峰佛山科学技术学院生命科学与工程学院广东佛山528225 华南农业大学兽医学院

目的利用原核表达系统表达华南地区分离的三源重组H9N2亚型禽流感病毒(Avian influenza virus,AIV)的神经氨酸酶(NA)蛋白,并制备兔多克隆抗体。方法将1株H9N2亚型AIV的NA基因克隆到pET-32a载体,构建NA原核表达质粒,并转化至*** Rosetta(... 详细信息

目的利用原核表达系统表达华南地区分离的三源重组H9N2亚型禽流感病毒(Avian influenza virus,AIV)的神经氨酸酶(NA)蛋白,并制备兔多克隆抗体。方法将1株H9N2亚型AIV的NA基因克隆到pET-32a载体,构建NA原核表达质粒,并转化至*** Rosetta(DE3)感受态细胞进行蛋白表达。优化IPTG浓度、诱导时间和温度,提高蛋白表达量。利用镍柱纯化重组NA蛋白,并免疫新西兰兔,制备兔抗NA蛋白多克隆抗体通过Western blot和间接免疫荧光试验(Indirect immunofluorescence assay,IFA)验证多克隆抗体的特异性。通过间接酶联免疫吸附试验(Enzyme-linked immunosorbent assay,ELISA)测定多克隆抗体的效价。结果成功构建了pET-32a-H9N2-NA原核表达质粒;经SDS-PAGE和Western blot验证,表达的重组NA蛋白大小约70 ku,符合预期;对诱导条件进行优化,确定37℃,终浓度为1.2 mmol/L的IPTG,诱导时长6 h为最佳条件;制备的兔抗NA蛋白多克隆抗体的Western blot和IFA的结果显示,该多克隆抗体能和H9N2亚型AIV结合,特异性好;间接ELISA测得兔抗NA蛋白多克隆抗体的效价达到了1∶409600。结论本研究表达的NA蛋白,制备的兔抗NA蛋白多克隆抗体具有特异性,效价高,为NA蛋白的ELISA检测方法的建立和疫苗研制提供帮助。

关键词：禽流感病毒 H9N2亚型神经氨酸酶原核表达多克隆抗体

An improved lightweight network based on deep learning for grape recognition in unstructured environments

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Information Processing in Agriculture 2024年第2期11卷 202-216页

作者： Bingpiao liu yunzhi Zhang Jinhai Wang Lufeng Luo Qinghua Lu Huiling Wei Wenbo Zhu School of Mechatronic Engineering and Automation Foshan UniversityFoshan 528000China

In unstructured environments,dense grape fruit growth and the presence of occlusion cause difficult recognition problems,which will seriously affect the performance of grape picking *** address these problems,this study improves the YOLOx-Tiny model and proposes a new grape detection model,YOLOX-RA,which can quickly and accurately identify densely growing and occluded grape *** proposed YOLOX-RA model uses a 3×3 convolutional layer with a step size of 2 to replace the focal layer to reduce the computational *** CBS layer in the ResBlock_Body module of the second,third,and fourth layers of the backbone layer is removed,and the CSPLayer module is replaced by the ResBlock-M module to speed up the *** auxiliary network(AlNet)with the remaining network blocks was added after the ResBlock-M module to improve the detection *** depth-separable convolutions(DsC)are used in the neck module layer to replace the normal convolution to reduce the computational *** evaluated the detection performance of SSD,YOLOv4 SSD,YOLOv4-Tiny,YOLO-Grape,YOLOv5-X,YOLOX-Tiny,and YOLOX-RA on a grape test *** results show that the YOLOX-RA model has the best detection performance,achieving 88.75%mAP,a recognition speed of 84.88 FPS,and model size of 17.53 *** can accurately detect densely grown and shaded grape bunches,which can effectively improve the performance of the grape picking robot.

关键词： YOLOX Grape recognition Depthwise separable convolution Res Block-M AINet

CREBBP基因突变致Rubinstein-Taybi综合征1例

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中国优生与遗传杂志 2024年第3期32卷 535-540页

作者：梁冬琳韦拔韦义军蒋永江刘芸芝黄雪美韦立袁德健柳州市妇幼保健院新生儿科广西柳州545001 柳州市妇幼保健院医学遗传科广西柳州545616 广州市妇女儿童医疗中心柳州医院医学遗传科广西柳州545616

目的研究Rubinstein-Taybi综合征(RSTS)的临床表现及遗传学特征。方法回顾分析1例经基因检测最终确诊为RSTS患儿的临床资料并复习相关文献。结果患儿女,1月婴儿,具有头发浓密,额部、颈部毛细血管畸形、双眼异常(外观:弓拱形浓眉,眼距增... 详细信息

目的研究Rubinstein-Taybi综合征(RSTS)的临床表现及遗传学特征。方法回顾分析1例经基因检测最终确诊为RSTS患儿的临床资料并复习相关文献。结果患儿女,1月婴儿,具有头发浓密,额部、颈部毛细血管畸形、双眼异常(外观:弓拱形浓眉,眼距增宽,内眦赘皮,外下斜眼裂;眼部见视盘缺失,视神经囊状增粗)、拇指(趾)宽大畸形等疑似RSTS临床症状,经全外显子基因测序显示CREBBP(NM_004380)基因存在c.4735C>T杂合突变,为无义变异,该突变经ACMG评定为致病,且该突变尚未见报道。结论RSTS主要表现为特殊面部畸形、肢端宽大畸形等,易合并眼部视网膜功能障碍等并发症,且患肿瘤风险性增加等,CREBBP和EP300基因为常见致病基因,可借助外显子基因测序协助诊断。

关键词：鲁宾斯坦-泰比综合征 CREBBP 宽拇指(趾) 婴幼儿

模糊需求下考虑供应商公平偏好的VMI供应链协调

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系统工程理论与实践 2016年第7期36卷 1661-1675页

作者：刘云志樊治平东北大学工商管理学院沈阳110167

针对模糊需求且考虑供应商公平偏好的二级VMI(vendor managed inventory)供应链,依据Cui等提出的不公平厌恶模型,分析了此VMI供应链在批发价格契约下的协调情况.得到的主要结论是:在不利不公平分配情形下,公平偏好供应商存在唯一的最优... 详细信息

针对模糊需求且考虑供应商公平偏好的二级VMI(vendor managed inventory)供应链,依据Cui等提出的不公平厌恶模型,分析了此VMI供应链在批发价格契约下的协调情况.得到的主要结论是:在不利不公平分配情形下,公平偏好供应商存在唯一的最优产品生产量,其为公平偏好系数(零售商势力外生参数)的严格减函数(减函数)且不高于风险中性的供应商的最优产品生产量,批发价格契约无法使模糊需求下考虑供应商公平偏好的二级VMI供应链达到协调;在有利不公平分配情形下,公平偏好供应商存在唯一的最优产品生产量,其为公平偏好系数(零售商势力外生参数)的严格增函数(增函数)且不低于风险中性的供应商的最优产品生产量,批发价格契约在一定条件下可使模糊需求下考虑供应商公平偏好的二级VMI供应链达到协调.最后,通过数值实验分析验证了相关研究结论.

关键词：供应链协调供应商管理库存(VMI) 模糊需求公平偏好批发价格契约

Genome wide association analysis identifies candidate genes for fruit quality and yield in Actinidia eriantha

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Journal of Integrative Agriculture 2024年第6期23卷 1929-1939页

作者： Yingzhen Wang Ying Wu Xinlei Wang Wangmei Ren Qinyao Chen Sijia Zhang Feng Zhang yunzhi Lin Junyang Yue Yongsheng liu School of Horticulture Anhui Agricultural UniversityHefei 230036China School of Forestry Science and Technology Lishui Vocational and Technical CollegeLishui 323000China College of Life Science Sichuan UniversityChengdu 610064China

Quality and yield are the primary concerns in kiwifruit breeding,but research on the genetic mechanisms of fruit size,shape,and ascorbic acid(ASA)content is currently very limited,which restricts the development of kiwifruit molecular *** this study,we obtained a total of 8.88 million highly reliable single nucleotide polymorphism(SNP)markers from 140 individuals from the natural hybrid offspring of Actinidia eriantha cv.‘White’using whole genome resequencing technology.A genome-wide association study was conducted on eight key agronomic traits,including single fruit weight,fruit shape,ASA content,and the number of inflorescences per branch.A total of 59 genetic loci containing potential functional genes were located,and candidate genes related to single fruit weight,fruit length,ASA content,number of inflorescences per branch and other traits were identified within the candidate interval,such as AeWUSCHEL,AeCDK1(cell cycle dependent kinase),AeAO1(ascorbic oxidase)and AeCO1(CONSTANS-like 4).After constructing an RNAi vector for AeAO1 and injecting it into the fruit of cv.‘Midao 31’to interfere with the expression of the AeAO1 gene,the results showed that the activity of ascorbic oxidase in the fruit of‘Midao 31’significantly decreased,while the content of ASA significantly *** study provides valuable insights into the genetic basis of variation in *** fruit traits,which may benefit molecular marker-assisted breeding efforts.

关键词： Actinidia eriantha GWAS SNP QTLs fruit quality