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Performance and Modeling of an Up-flow Anaerobic Sludge Blanket(UASB) Reactor for Treating High Salinity Wastewater from Heavy Oil Production

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China Petroleum Processing & Petrochemical Technology 2012年第3期14卷 90-95页

作者： liu chunshuang Zhao Dongfeng Guo Yadong Zhao Chaocheng College of Chemical Engineering China University of Petroleum

In this study,an up-flow anaerobic sludge blanket(UASB) reactor was applied to treat the high salinity wastewater from heavy oil production *** a HRT of ≥24 h,the COD removal reached as high as 65.08% at an influent COD ranging from 350mg/L to 640mg/*** average of 74.33% oil reduction was also achieved in the UASB reactor at an initial oil concentration between 112mg/L and 205mg/*** results indicated that this heavy oil production related wastewater could be degraded efficiently in the UASB *** sludge was formed in this *** addition,two models,built on the back propagation neural network(BPNN) theory and linear regression techniques were developed for the simulation of the UASB system performance in the oily wastewater *** average error of COD and oil removal was-0.65% and 0.84%,*** results indicated that the models built on the BPNN theory were wellfitted to the detected data,and were able to simulate and predict the removal of COD and oil by the UASB reactor.

关键词： up-flow anaerobic sludge blanket (UASB) high salinity heavy oil produced wastewater granule sludge BPNN

Performance Evaluation of the Halophilic Crude Oildegrading Bacterial Consortium XH-1 for Oilfield Wastewater Treatment

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China Petroleum Processing & Petrochemical Technology 2024年第3期26卷 24-32页

作者： Gong Xihui Zhang Manlin Wang Wenna Sun Zhichao liu chunshuang College of Chemistry and Chemical Engineering China University of Petroleum(East China)Qingdao 266580China

A total of 14 halophilic hydrocarbon-degrading strains were isolated from crude oil-contaminated sites,using petroleum as the sole carbon and energy *** these,four highly efficient strains were selected to create the mixed bacterial agent *** four strains were identified through 16S rRNA gene-based sequencing as belonging to Acinetobacter,Bacillus paramycoides,Rhodococcus sp.,and Enterobacter sp.,*** optimal cultivation time for the mixed consortium XH-1 was found to be 48 h,and a nitrogen-phosphorus molar ratio of 10:1 was determined to be beneficial for crude oil ***-1 showed notable crude oil degradation even at a salinity of up to 30 g/L,with little inhibition observed at sulfide concentrations as high as 150 mg/L and initial oil concentrations of 500 mg/*** chromatography analysis revealed that XH-1 was able to efficiently degrade C9–C29 ***,a bio-contact oxidation reactor enhanced by XH-1 showed promising results in treating oilfield *** findings suggest that XH-1 can be applied for the treatment of oilfield wastewater.

关键词： crude oil degradation mixed strains oilfield wastewater degradation characteristics

Enhanced Performance of Denitrifying Sulfide Removal Process by 1,2-Naphthoquinone-4-Sulphonate

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China Petroleum Processing & Petrochemical Technology 2016年第1期18卷 22-28页

作者： liu chunshuang Han Kang Zhao Dongfeng Guo Yadonag liu Lihong liu Fang Zhao Chaocheng College of Chemical Engineering China University of PetroleumQingdao School of Earth Sciences Northeast Petroleum University

The denitrifying sulfide removal(DSR) process with bio-granules comprising both heterotrophic and autotrophic denitrifiers can simultaneously convert nitrate, sulfide and acetate species into di-nitrogen gas, elemental sulfur and carbon dioxide, respectively, at high loading rates. This study has determined that the reaction rate of sulfide oxidized into sulfur could be enhanced in the presence of 1,2-naphthoquinone-4-sulphonate(NQS). The presence of NQS mitigated the inhibition effects of sulfide species on denitrification. Furthermore, the reaction rates of nitrate and acetate to nitrogen gas and CO_2, respectively, were also promoted in the presence of NQS, thereby enhancing the performance of DSR granules. The advantages and disadvantages of applying the NQS-DSR process are discussed.

关键词： denitrifying sulfide removal NQS inhibition bio-granules

Effects of Fe^(2+), Co^(2+) and Ni^(2+) Ions on Biological Methane Production from Residual Heavy Oil

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China Petroleum Processing & Petrochemical Technology 2015年第2期17卷 32-38页

作者： liu chunshuang Ma Wenjuan Zhao Dongfeng Jia Kuili Zhao Chaocheng College of Chemical Engineering China University of Petroleum

On the basis of single factor tests, the effect of trace elements—Fe2+, Co2+ and Ni2+ ions—on biological methane production from heavy oil was investigated by the response surface method. A three-level Box-Behnken design was employed to study the relationship between the independent variables and the dependent variable by applying initial Fe2+, Co2+ and Ni2+ concentration as the independent variables(factors) and using the methane production after 270 days of cultivation as the dependent variable(response). A prediction model of quadramatic polynomial regression equation was obtained. The results showed that the methane production could be as high as 240.69 μmol after optimization compared with 235.74 μmol obtained under un-optimized condition. Furthermore, the microbial communities before and after biodegradation were analyzed by PCR-DGGE method. The dominant bands were recovered and sequenced. Three strains were obtained; the strain T1 has 97% similarity with Bacillus thermoamylovorans, the strain H3 has 97% similarity with Bacillus thermoamylovorans and the strain H4 has 99% similarity with Bacillus vietnamensis.

关键词： residual heavy oil methane PCR-DGGE response surface method

Optimization for Microbial Degradation of Dibenzothiophene by Pseudomonas sp. LKY-5 Using Response Surface Methodology

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China Petroleum Processing & Petrochemical Technology 2014年第1期16卷 19-26页

作者： Li Lin Zhao Chaocheng liu Qiyou Zhang Yunbo liu chunshuang Xue Jianliang College of Chemical Engineering China University of Petroleum Qingdao

In this research, the degradation of dibenzothiophene(DBT) was investigated by using Pseudomonas sp. LKY-5 isolated from oil contaminated soil. The response surface methodology(RSM) based on the Box-Behnken design(BBD) was applied for evaluating the interactive effects of four independent variables including substrate concentration, temperature, pH and agitation rate on the DBT removal response. A total of 29 experiments for four factors at three levels were conducted in present study. A second-order regression model was then developed, and the analysis of variance(ANOVA) illustrated that the proposed quadratic model could be utilized to navigate the design space. The value of determination coefficient(R2=0.953 4) indicated a satisfactory agreement between the quadratic model and the experimental data. It was found that DBT removal was more significantly affected(P<0.000 1) by substrate concentration compared with other three parameters. An 100% degradation of DBT could be obtained by Pseudomonas sp. LKY-5 at a substrate concentration of 100 mg/L.

关键词： degradation dibenzothiophene Pseudomonas sp. response surface methodology Box–Behnken design

Complete Nitrogen Removal through Integrating Anammox and Autotrophic Denitrification in an UASB Reactor

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China Petroleum Processing & Petrochemical Technology 2020年第1期22卷 111-117页

作者： Guo Yadong Bai Xue Yu Haitong Li Xuechen Zhao Chaocheng liu chunshuang College of New Energy China University of PetroleumQingdao 266580 College of Chemical Engineering China University of PetroleumQingdao 266580

Complete nitrogen removal was achieved through integrating anammox and autotrophic denitrification in an UASB *** total nitrogen(TN)removal rate increased stepwise from 0.46 to 0.94 kg-N/(m3·d),with an effluent TN concentration of below 3.0 mg-N/L *** process is relatively insensitive to the nitrite to ammonium ratio,achieving complete nitrogen removal when their ratio in the influent varied in the range of *** added S0 quantity in the system could be utilized to adjust the competition between autotrophic denitrifiers and anammox ***-throughput sequencing technology indicated that Candidatus_Kuenenia and Thiobacillus were the functional strains for anammox and autotrphic denitrification process,respectively,in the studied *** result provides a theoretical and technical basis for the large-scale application of anaerobic ammonium oxidation process.

关键词： complete nitrogen removal anammox elemental sulfur UASB

Nitrogen Removal Performance of Denitrifying Ammonium Oxidation System in Treating Sulfamethoxazole-laden Secondary Wastewater Effluent

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China Petroleum Processing & Petrochemical Technology 2021年第1期23卷 105-110页

作者： liu chunshuang Li Wei Duan Weichao Huiyun Zhong Yu Haitong Li Yanze liu Fang Zhao Chaocheng College of Chemical Engineering China University of PetroleumQingdao 266580 Qingdao Oasis Environmental&Safety Technology Co. LtdQingdao 266555 School of Environmental and Safety Engineering Changzhou UniversityChangzhou213164

In this study,nitrogen removal performance of the denitrifying ammonium oxidation(DAO)process was investigated when treating sulfamethoxazole(SMX)-laden secondary wastewater *** influent SMX concentration showed negligible effect on efficiencies for removal of nitrate and ***,the ammonium ions removal rate was moderately reduced,when the influent SMX concentration in wastewater reached 6 mg/*** nitrogen removal efficiency remained as high as 76.77%towards the day 158 at the end of ***_Brocadia and Candidatus_Kuenenia were the functional anammox *** unclassified_f__Rhodobacteraceae *** predominant heterotrophic denitrifying strain in the studied *** concentrations of soluble extracellular polymeric substances in sludge obviously increased from 16.76 mg/g VSS to 32.31 mg/g VSS,which might protect the nitrogen removal strains from high-concentration *** result provides a theoretical and technical foundation for the application of denitrifying ammonium oxidation process in treating sulfamethoxazole-laden secondary wastewater effluent.

关键词： denitrifying ammonium oxidation sulfamethoxazole extracellular polymeric substances total nitrogen

Simultaneous Removal of Sulfide, Nitrate and Ammonium Ions from Wastewater in One Reactor

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China Petroleum Processing & Petrochemical Technology 2019年第4期21卷 68-72页

作者： liu chunshuang Li Wei Bai Xue Wang Yongxing Wang Yongqiang liu Fang Zhao Chaocheng State Key Laboratory of Petroleum Pollution Control China University of PetroleumQingdao 266580 College of Chemical Engineering China University of PetroleumQingdao 266580

Simultaneous removal of sulfide, nitrate and ammonium salts was achieved in an upflow anaerobic sludge blanked(UASB) reactor by the start-up strategy of gradually decreasing influent nitrite to null. The sulfide/nitrate-nitrogen ratio could affect the distribution of end products of sulfur, and a sulfide/nitrate-nitrogen ratio of 1:0.76 is proposed as optimum for S^0 accumulation. The Denittrasoma, Thiobacillus, and Candidatus_Kuenenia presented the functional strains in the studied reactor. This result can provide a theoretical and technical basis for the large-scale application of anaerobic ammonium oxidation process.

关键词： nitrate sulfide ammonium anammox

Enhanced Mineralization of p-Fluoronitrobenzene in a Methane-based Hollow-fiber Membrane Biofilm Reactor

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China Petroleum Processing & Petrochemical Technology 2021年第2期23卷 60-65页

作者： liu chunshuang Yu Haitong Qu Changyong Li Wei Wang Yannan Guo Yadong HanFenglei College of Chemical Engineering China University of PetroleumQingdao 266580 SINOPEC Jinan Refning&Chemical Company Jinan 250014

In this study,the enhanced mineralization of p-fluoronitrobenzene(p-FNB)was achieved in a methane-based hollow-fiber membrane biofilm reactor(CH4-MBfR).A TOC removal of 73.2%was gained in CH4-MBfR with an influent p-FNB loading of 42―84 mg/L·d,while only 40.6%of TOC was removed in the corresponding control biological reactor(BR).Moreover,the reaction rate constant for TOC removal in CH4-MBfR was 2.48 times that of the *** was only detected in CH4-MBfR,although oxidation elimination was the first step for p-FNB removal in both *** and Methylononas were dominant strains in the CH4-MBfR,which may play an important role in converting CH4 to VFAs and then further enhancing the mineralization efficiency of p-FNB.

关键词： p-fluoronitrobenzene biodegradation CH4-MBfR mineralization