Optimization for Microbial Degradation of Dibenzothiophene by Pseudomonas sp. LKY-5 Using Response Surface Methodology

作     者：Li Lin Zhao Chaocheng Liu Qiyou Zhang Yunbo Liu Chunshuang Xue Jianliang 

作者机构：College of Chemical Engineering China University of Petroleum Qingdao 

出 版 物：《China Petroleum Processing & Petrochemical Technology》 (中国炼油与石油化工（英文版）)

年 卷 期：2014年第16卷第1期

页      面：19-26页

核心收录：

学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 

基　　金：support provided by the Fundamental Research Funds for the Central Universities (No. 12CX06043A) of China the Key Laboratory of Marine Spill Oil Identification and Damage Assessment Technology,SOA(No. 201407) 

主　　题：degradation dibenzothiophene Pseudomonas sp. response surface methodology Box–Behnken design 

摘      要：In this research, the degradation of dibenzothiophene(DBT) was investigated by using Pseudomonas sp. LKY-5 isolated from oil contaminated soil. The response surface methodology(RSM) based on the Box-Behnken design(BBD) was applied for evaluating the interactive effects of four independent variables including substrate concentration, temperature, pH and agitation rate on the DBT removal response. A total of 29 experiments for four factors at three levels were conducted in present study. A second-order regression model was then developed, and the analysis of variance(ANOVA) illustrated that the proposed quadratic model could be utilized to navigate the design space. The value of determination coefficient(R2=0.953 4) indicated a satisfactory agreement between the quadratic model and the experimental data. It was found that DBT removal was more significantly affected(P0.000 1) by substrate concentration compared with other three parameters. An 100% degradation of DBT could be obtained by Pseudomonas sp. LKY-5 at a substrate concentration of 100 mg/L.