Effects of Fe^(2+), Co^(2+) and Ni^(2+) Ions on Biological Methane Production from Residual Heavy Oil

作     者：Liu Chunshuang Ma Wenjuan Zhao Dongfeng Jia Kuili Zhao Chaocheng 

作者机构：College of Chemical Engineering China University of Petroleum 

出 版 物：《China Petroleum Processing & Petrochemical Technology》 (中国炼油与石油化工（英文版）)

年 卷 期：2015年第17卷第2期

页      面：32-38页

核心收录：

学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 

基　　金：supported by the National Natural Science Foundation of China under Grant No.21307160 the Natural Science Foundation of Shandong Province under Grant No.ZR2013EEQ030 the Fundamental Research Funds for the Central Universities under Grant No.R1404005A 

主　　题：residual heavy oil methane PCR-DGGE response surface method 

摘      要：On the basis of single factor tests, the effect of trace elements—Fe2+, Co2+ and Ni2+ ions—on biological methane production from heavy oil was investigated by the response surface method. A three-level Box-Behnken design was employed to study the relationship between the independent variables and the dependent variable by applying initial Fe2+, Co2+ and Ni2+ concentration as the independent variables(factors) and using the methane production after 270 days of cultivation as the dependent variable(response). A prediction model of quadramatic polynomial regression equation was obtained. The results showed that the methane production could be as high as 240.69 μmol after optimization compared with 235.74 μmol obtained under un-optimized condition. Furthermore, the microbial communities before and after biodegradation were analyzed by PCR-DGGE method. The dominant bands were recovered and sequenced. Three strains were obtained; the strain T1 has 97% similarity with Bacillus thermoamylovorans, the strain H3 has 97% similarity with Bacillus thermoamylovorans and the strain H4 has 99% similarity with Bacillus vietnamensis.