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The expression of lin28B was co-regulated by H3K4me2 and Wnt5a/β-catenin/TCF7L2

Journal of Integrative Agriculture 2020年第12期19卷 3054-3064页

作者： ZHANG Ya-ni HU Cai WANG Ying-jie ZUO Qi-sheng li bi-chun Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province College of Animal Science and TechnologyYangzhou UniversityYangzhou 225009P.R.China Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education Yangzhou UniversityYangzhou 225009P.R.China

lin28A and lin28B are homologous RNA-binding proteins that participate in the development of primordial germ cells. The mechanisms underlying expression and regulation of lin28A have been well documented, but such information for lin28B is limited. In this study, a fragment of the lin28B promoter was cloned, the pEGFP-plin28B vector was constructed. DF-1 chicken fibroblasts were transfected and the expression of green fluorescent protein (GFP) was measured. Furtherly, lin28B promoter of different lengths fragments was cloned using the chromosome-walking method and the fragments were ligated into the PGL3-Basic vector, and transfected into DF-1 cells. Results of dual-luciferase reporter assay showed that the core of the lin28B promoter was included in the sequence from –1 431 to –1 034 bp. The binding sites of the transcription factor TCF7L2 was showed within this sequence by bioinformatics analysis. The promoter activity of lin28B was downregulated (Pbinding site was mutated. Further experiments suggested that lin28B promoter activity responded to the activation or inhibition of Wnt signaling. Results of chromatin immunoprecipitation and quantitative PCR showed that β-catenin-TCF7L2 may be enriched in the lin28B promoter core area. In vivo and in vitro activation or inhibition of Wnt signaling significantly up- or down-regulated (Plin28B expression. H3K4me2 enriched in the promoter of lin28B, which affected the regulation of Wnt signaling to lin28B. In conclusion, our results showed that H3K4me2 and Wnt5a/β-catenin/TCF7L2 were the positive regulators of lin28B expression. Findings of this study may lay a theoretical foundation for illuminating the mechanism underlying lin28B expression.

关键词： primordial germ cells lin28B promoter H3K4me2 Wnt5a/β-catenin/TCF7L2

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Study on the role of JAK/STAT signaling pathway during chicken spermatogonial stem cells generation based on RNA-Seq

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Journal of Integrative Agriculture 2015年第5期14卷 939-948页

作者： ZHANG Lei ZUO Qi-sheng li Dong liAN Chao Kamel EAhmed TANG Bei-bei SONG Jiu-zhou ZHANG Ya-ni li bi-chun Jiangsu Province Key Laboratory of Animal Breeding and Molecular Design College of Animal Science and TechnologyYangzhou University Department of Anatomy and Embryology College of Veterinary MedicineSuez Canal University Animal & Avian Sciences University of Maryland

Spermatogonia! stem cells（SSCs） form the foundation for spermatogenesis and sustain male fertility.To explore the regulatory mechanisms of chicken SSCs generation,we obtained highly purified chicken embryonic stem cells（ESCs）,primordial germ cells（PGCs） and SSCs by fluorescence-activated cell sorting（FACS）.High-throughput analysis methods（RNA-Seq） were used to sequence the transcriptome level of these cells.Gene ontology and Kyoto encyclopedia of genes and genomes（KEGG） pathway enrichment were used to analyze RNA-Seq results.BMP4 was used to induce chicken ESCs differentiation to SSCs-like cells in vitro.The quantitative real-time（qRT）-PCR was used to detect the expression changes of the key genes.The results showed that 22 relevant critical pathways were found by RNA-Seq,one of them was the Janus kinase/signal transducer and activator of transcription（JAK/STAT） signaling pathway.Total of 103 related genes were detected in this pathway.Protein-protein interactions analysis found that 87 proteins were significantly related to 19 key proteins in this pathway.These 87 proteins were enriched in 21 biological processes and 18 signaling pathways.Moreover,during the differentiation of chicken ESCs to SSCs-like cells induced by BMP4 in vitro,JAK2 and STAT3 were activated.The qRT-PCR results showed that the expression trends of JAK2 and STAT3 were basically the same as in vivo.We concluded that JAK/STAT signaling pathway plays an important role in the process of chicken SSCs generation both in vivo and in vitro;it may achieve its function through multiple biological processes and other related pathways.

关键词： chicken signaling PGCs spermatogonial fertility primordial embryonic RNA sorting concluded

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UBE2I stimulates female gonadal differentiation in chicken(Gallus gallus)embryos

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Journal of Integrative Agriculture 2021年第11期20卷 2986-2994页

作者： JIN Kai ZHOU Jing ZUO Qi-sheng li Jian-cheng Jiuzhou SONG ZHANG Ya-ni CHANG Guo-bing CHEN Guo-hong li bi-chun Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province College of Animal Science and TechnologyYangzhou UniversityYangzhou 225009P.R.China Institutes of Agricultural Science and Technology Development Yangzhou UniversityYangzhou 225009P.R.China Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China Yangzhou UniversityYangzhou 225009P.R.China Animal&Avian Sciences University of MarylandCollege ParkMD20741USA

Without known analogous sex-determining factors like SRY(sex determining region Y)in mammals,the chicken(Gallus gallus)sex determination mechanism still remains unclear,which highly restricts the biological research on chicken development and poultry single-sex reproduction.Here we not only characterized a new female-biased gene UBE2I and identified the expression pattern by qRT-PCR,but also described the functional role of UBE2I in the gonadal development of chicken embryos.Results showed that UBE2I exhibited a female-biased expression pattern in the early stage of PGCs(primordial germ cells)in embryonic gonads and robust expression in ovaries of newborn chickens.Most importantly,we successfully developed an effective method to interfere or overexpress UBE2I in chicken embryos through the intravascular injection.The qRT-PCR analysis showed that the sex-related genes(FOXL2,CYP19A1 and HINTW)in females were upregulated(P<0.05)under the overexpression of UBE2I and the sex-related genes(SOX9,DMRT1 and WT1)in females were downregulated(P<0.05)after interfering UBE2I.Furthermore,the change of UBE2I expression was associated with the level of estradiol and its receptors(AR and ESR),which suggests that UBE2I is necessary to initiate the female-specific development in chickens.In conclusion,this work demonstrates that UBE2I is a crucial sex differentiation-related gene in the embryonic development of chickens,which provides insights for further understanding the mechanism of sex determination in chickens.

关键词： UBE2I gonadal differentiation chicken embryonic sex differentiation

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Research on the appropriate way to transfer exogenous substances into chicken embryos

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Journal of Integrative Agriculture 2017年第10期16卷 2257-2263页

作者： WANG Yi-lin JIN Kai HE Na-na CHENG Shao-ze ZUO Qi-sheng li Dong WANG Ying-jie WANG Fei JI Yan-qing LU Zhen-yu ZHANG Chen WANG Man ZHAO Rui-feng YU Xin-jian ZHANG Ya-ni ZHAO Wen-ming li bi-chun College of Animal Science and Technology/Jiangsu Province Key Laboratory of Animal Breeding and Molecular Design Yangzhou University Yangzhou 225009 P.R.China

In biological research, chicken embryos are a classic experimental model for the exploration of the embryonic development and cell differentiation. Transferring exogenous substances into chicken embryos for producing medical antibodies has been widely used in the production practice. However, there are few studies about the effect of the different injection site and dosage on chicken embryos. The aim of this study was to explore the effects of different injection sites and dosages on chicken embryo hatching rate and development, so as to provide a basis for further studies using the chicken embryo model. Freshly laid eggs （Rugao yellow chicken） were injected with different doses of saline at the tip, equatorial plane and the blunt end of the egg shell, respectively. Egg hatching rate was recorded and compared among injection sites and different doses. A trypan blue stain was also injected at the aforementioned sites and the growth of chicken embryos was observed. The SPSS （statistical package for the social science） software was used to analyze the relationship between the chicken eggs hatching rate and the different injection sites or the different dosages. The experimental results showed that there were significant differences on egg hatching rates among the different injection sites and doses （P〈0.05）. The hatchability of the blunt end injection group was significantly higher than that of the other two sites. The egg hatching rate decreased with increased saline doses. The egg hatching rate of the 100 pL saline injection group was higher than the 200 and 300 μL dosage groups. Ultimately, we suggest that the optimal chicken embryo injection process is during early development, at the blunt end site with a dose less than 100 μL to minimize damage to the egg.

关键词： chicken embryos injection dosage hatchability chick embryo development

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Vinyltrimethylsilane as a novel electrolyte additive for improving interfacial stability of li-rich cathode working in high voltage

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Chinese Chemical Letters 2024年第2期35卷 534-538页

作者： bing Jiang Hao li bi Luo Lehao liu lihua Chu Qiaobao Zhang Meicheng li State Key Laboratory of Alternate Electrical Power System with Renewable Energy Sources School of New EnergyNorth China Electric Power UniversityBeijing 102206China State Key Laboratory of Physical Chemistry of Solid Surfaces College of MaterialsXiamen UniversityXiamen 361005China

Boosting the interfacial stability between electrolyte and li-rich cathode material at high operating voltage is vital important to enhance the cycling stability of li-rich cathode materials for high-performance li-ion batteries.In this work,vinyltrimethylsilane as a new type of organic silicon electrolyte additive is studied to address the interfacial instability of li-rich cathode material at high operating voltage.The cells using vinyltrimethylsilane additive shows the high capacity retention of 73.9%after 300 cycles at 1 C,whereas the cells without this kind of additive only have the capacity retention of 58.9%.The improvement of stability is mainly attributed to the additive helping to form a more stable surface film for li-rich cathode material,thus avoiding direct contact between the electrolyte and the cathode material,slowing down the dissolution of metal ions and the decomposition of the electrolyte under high operating voltage.Our findings in this work shed some light on the design of stable cycling performance of li-rich cathode toward advanced li-ion batteries.

关键词： Vinyltrimethylsilane Electrolyte additive li-rich cathode Interfacial stability Capacity retention

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Trade-offs between the accumulation of cuticular wax and jasmonic acid-mediated herbivory resistance in maize

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Journal of Integrative Plant biology 2024年第1期66卷 143-159页

作者： Jiong liu Lu li Zhilong Xiong Christelle AMRobert Baozhu li Shan He Wenjie Chen Jiasheng bi Guanqing Zhai Siyi Guo Hui Zhang Jieping li Shutang Zhou Xi Zhang chun‐Peng Song School of life Sciences Henan UniversityKaifeng 475004China State Key Laboratory of Crop Stress Adaptation and Improvement Henan UniversityKaifeng 475004China State Key Laboratory of Cotton bio‐Breeding and Integrated Utilization Henan UniversityKaifeng 475004China Sanya Institute of Henan University Sanya 572025China Institute of Plant Sciences University of BernBern 3013Switzerland

Plants have evolved complex physical and chemical defense systems that allow them to withstand herbivory infestation.Composed of a complex mixture of very-long-chain fatty acids(VLCFAs)and their derivatives,cuticular wax constitutes the first physical line of defense against herbivores.Here,we report the function of Glossy 8(ZmGL8),which encodes a 3-ketoacyl reductase belonging to the fatty acid elongase complex,in orchestrating wax production and jasmonic acid(JA)-mediated defenses against herbivores in maize(Zea mays).The mutation of GL8 enhanced chemical defenses by activating the JA-dependent pathway.We observed a trade-off between wax accumulation and JA levels across maize glossy mutants and 24 globally collected maize inbred lines.In addition,we demonstrated that mutants defective in cuticular wax biosynthesis in Arabidopsis thaliana and maize exhibit enhanced chemical defenses.Comprehensive transcriptomic and lipidomic analyses indicated that the gl8 mutant confers chemical resistance to herbivores by remodeling VLCFA-related lipid metabolism and subsequent JA biosynthesis and signaling.These results suggest that VLCFA-related lipid metabolism has a critical role in regulating the trade-offs between cuticular wax and JA-mediated chemical defenses.

关键词： cuticular wax fall armyworm herbivore resistance jasmonic acid maize plant-herbivore interactions

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Anti-OX40 Antibody Combined with HBc VLPs Delays Tumor Growth in a Mouse Colon Cancer Model

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biomedical and Environmental Sciences 2024年第2期37卷 187-195页

作者： liU Jia Jia SU Qiu Dong YI Yao SHEN li Ping bi Sheng li National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China

Objective Combination immunotherapy strategies targeting OX40,a co-stimulatory molecule that can enhance antitumor immunity by modulating the proliferation,differentiation,and effector function of tumor-infiltrating T cells,have attracted much attention for their excellent therapeutic effects.In this study,we aimed to evaluate the antitumor efficacy of combined anti-OX40 and hepatitis B core viruslike particles(HBc VLPs)therapy using a mouse colon cancer model.Methods Humanized B-h OX40 mice were injected subcutaneously with MC38 colon tumor cells and treated with HBc VLPs+anti-h OX40 antibody.Tumor growth was monitored.Flow cytometric analysis was performed to evaluate the populations of T cell subsets in the tumors.Results The combination of anti-OX40 with HBc VLPs resulted in a significant delay in tumor growth,suggesting that a potent antitumor immunity was induced by the combination therapy.Further studies revealed that HBc VLPs+anti-OX40 treatment induced a significant increase in effector T cells(Teffs)and a significant decrease in regulatory T cells(Tregs)in the tumor microenvironment(TME),which accounted for the synergistic antitumor effect of anti-OX40 in combination with HBc VLPs.Conclusion Combination therapy of anti-h OX40 and HBc VLPs provides synergistic antitumor activity in colon cancer-bearing mice,which may represent a potential design strategy for cancer immunotherapy.

关键词： Anti-OX40 antibody Hepatitis B core virus-like particles Tumor Combination therapy

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Sparse Adversarial Learning for FDIA Attack Sample Generation in Distributed Smart

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Computer Modeling in Engineering & Sciences 2024年第5期139卷 2095-2115页

作者： Fengyong li Weicheng Shen Zhongqin bi Xiangjing Su College of Computer Science and Technology Shanghai University of Electric PowerShanghai201306China College of Electrical Engineering Shanghai University of Electric PowerShanghai201306China

False data injection attack(FDIA)is an attack that affects the stability of grid cyber-physical system(GCPS)by evading the detecting mechanism of bad data.Existing FDIA detection methods usually employ complex neural networkmodels to detect FDIA attacks.However,they overlook the fact that FDIA attack samples at public-private network edges are extremely sparse,making it difficult for neural network models to obtain sufficient samples to construct a robust detection model.To address this problem,this paper designs an efficient sample generative adversarial model of FDIA attack in public-private network edge,which can effectively bypass the detectionmodel to threaten the power grid system.A generative adversarial network(GAN)framework is first constructed by combining residual networks(ResNet)with fully connected networks(FCN).Then,a sparse adversarial learning model is built by integrating the time-aligned data and normal data,which is used to learn the distribution characteristics between normal data and attack data through iterative confrontation.Furthermore,we introduce a Gaussian hybrid distributionmatrix by aggregating the network structure of attack data characteristics and normal data characteristics,which can connect and calculate FDIA data with normal characteristics.Finally,efficient FDIA attack samples can be sequentially generated through interactive adversarial learning.Extensive simulation experiments are conducted with IEEE 14-bus and IEEE 118-bus system data,and the results demonstrate that the generated attack samples of the proposed model can present superior performance compared to state-of-the-art models in terms of attack strength,robustness,and covert capability.

关键词： Distributed smart grid FDIA adversarial learning power public-private network edge

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Feedback of Efficient Shock Acceleration on Magnetic-field Structure Inside Young Type Ia Supernova Remnants

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Research in Astronomy and Astrophysics 2024年第6期24卷 236-246页

作者： Jun-Yu Shen bi-Wen Bao li Zhang Department of Astronomy Key Laboratory of Astroparticle Physics of Yunnan Province Yunnan University Key Laboratory of Statistical Modeling and Data Analysis of Yunnan Province Yunnan University

Using an effective adiabatic index γeffto mimic the feedback of efficient shock acceleration, we simulate the temporal evolution of a young type Ia supernova remnant （SNR） with two different background magnetic field（BMF） topologies:a uniform and a turbulent BMF. The density distribution and magnetic-field characteristics of our benchmark SNR are studied with two-dimensional cylindrical magnetohydrodynamic simulations. When γeffis considered, we find that:（1） the two-shock structure shrinks and the downstream magnetic-field orientation is dominated by the Rayleigh–Taylor instability structures;（2） there exists more quasi-radial magnetic fields inside the shocked region; and （3） inside the intershock region, both the quasi-radial magnetic energy density and the total magnetic energy density are enhanced:in the radial direction, with γeff=1.1, they are amplified about 10–26 times more than those with γeff=5/3. While in the angular direction, the total magnetic energy densities could be amplified about 350 times more than those with γeff=5/3, and there are more grid cells within the intershock region where the magnetic energy density is amplified by a factor greater than 100.

关键词： ISM: magnetic fields ISM: supernova remnants magnetohydrodynamics (MHD) methods: numerical

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MAP4K inhibition as a potential therapy for amyotrophic lateral sclerosis

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Neural Regeneration Research 2024年第8期19卷 1639-1640页

作者： Shuaipeng Ma chun-li Zhang Department of Molecular biology University of Texas Southwestern Medical CenterDallasTXUSA Hamon Center for Regenerative Science and Medicine University of Texas Southwestern Medical CenterDallasTXUSA

Amyotrophic lateral sclerosis(ALS)is a rare neurological disease,featuring gradual loss of muscle controls due to degeneration of motor neurons.Unfortunately,there is currently no cure for ALS.The available therapies ... 详细信息

关键词： degeneration sclerosis MAP4

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