Study on the role of JAK/STAT signaling pathway during chicken spermatogonial stem cells generation based on RNA-Seq

作     者：ZHANG Lei ZUO Qi-sheng LI Dong LIAN Chao Kamel EAhmed TANG Bei-bei SONG Jiu-zhou ZHANG Ya-ni LI Bi-chun 

作者机构：Jiangsu Province Key Laboratory of Animal Breeding and Molecular DesignCollege of Animal Science and TechnologyYangzhou University Department of Anatomy and EmbryologyCollege of Veterinary MedicineSuez Canal University Animal & Avian SciencesUniversity of Maryland 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2015年第14卷第5期

页      面：939-948页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China(31272429,31472087) the Specialized Research Fund for the Doctoral Program of Higher Education,China(20123250120009) the China Postdoctoral Science Foundation Funded Project(2012M511326,2014T70550) the Research and Innovation Program for Graduate Cultivation of Jiangsu Province,China(CXZZ13_0909) the project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions,China 

主　　题：chicken signaling PGCs spermatogonial fertility primordial embryonic RNA sorting concluded 

摘      要：Spermatogonia! stem cells（SSCs） form the foundation for spermatogenesis and sustain male *** explore the regulatory mechanisms of chicken SSCs generation,we obtained highly purified chicken embryonic stem cells（ESCs）,primordial germ cells（PGCs） and SSCs by fluorescence-activated cell sorting（FACS）.High-throughput analysis methods（RNA-Seq） were used to sequence the transcriptome level of these *** ontology and Kyoto encyclopedia of genes and genomes（KEGG） pathway enrichment were used to analyze RNA-Seq ***4 was used to induce chicken ESCs differentiation to SSCs-like cells in *** quantitative real-time（qRT）-PCR was used to detect the expression changes of the key *** results showed that 22 relevant critical pathways were found by RNA-Seq,one of them was the Janus kinase/signal transducer and activator of transcription（JAK/STAT） signaling *** of 103 related genes were detected in this ***-protein interactions analysis found that 87 proteins were significantly related to 19 key proteins in this *** 87 proteins were enriched in 21 biological processes and 18 signaling ***,during the differentiation of chicken ESCs to SSCs-like cells induced by BMP4 in vitro,JAK2 and STAT3 were *** qRT-PCR results showed that the expression trends of JAK2 and STAT3 were basically the same as in *** concluded that JAK/STAT signaling pathway plays an important role in the process of chicken SSCs generation both in vivo and in vitro;it may achieve its function through multiple biological processes and other related pathways.