UBE2I stimulates female gonadal differentiation in chicken(Gallus gallus)embryos

作     者：JIN Kai ZHOU Jing ZUO Qi-sheng LI Jian-cheng Jiuzhou SONG ZHANG Ya-ni CHANG Guo-bing CHEN Guo-hong LI Bi-chun JIN Kai;ZHOU Jing;ZUO Qi-sheng;LI Jian-cheng;Jiuzhou SONG;ZHANG Ya-ni;CHANG Guo-bing;CHEN Guo-hong;LI Bi-chun

作者机构：Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu ProvinceCollege of Animal Science and TechnologyYangzhou UniversityYangzhou 225009P.R.China Institutes of Agricultural Science and Technology DevelopmentYangzhou UniversityYangzhou 225009P.R.China Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of ChinaYangzhou UniversityYangzhou 225009P.R.China Animal&Avian SciencesUniversity of MarylandCollege ParkMD20741USA 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2021年第20卷第11期

页      面：2986-2994页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：funded by the National Natural Science Foundation of China(31772582 and 31972547) the National Key R&D Program of China(2017YFE0108000) the High Level Talents Support Program of Yangzhou University,Postgraduate Research&Practice Innovation Program of Jiangsu Province,China(KYCX182376) the Jiangsu Science and Technology Project,China(Youth Fund,BK20180918) the Natural Science Research Project of Jiangsu Higher Education Institutions,China(18KJB230008) 

主　　题：UBE2I gonadal differentiation chicken embryonic sex differentiation 

摘      要：Without known analogous sex-determining factors like SRY(sex determining region Y)in mammals,the chicken(Gallus gallus)sex determination mechanism still remains unclear,which highly restricts the biological research on chicken development and poultry single-sex *** we not only characterized a new female-biased gene UBE2I and identified the expression pattern by qRT-PCR,but also described the functional role of UBE2I in the gonadal development of chicken *** showed that UBE2I exhibited a female-biased expression pattern in the early stage of PGCs(primordial germ cells)in embryonic gonads and robust expression in ovaries of newborn *** importantly,we successfully developed an effective method to interfere or overexpress UBE2I in chicken embryos through the intravascular *** qRT-PCR analysis showed that the sex-related genes(FOXL2,CYP19A1 and HINTW)in females were upregulated(P0.05)under the overexpression of UBE2I and the sex-related genes(SOX9,DMRT1 and WT1)in females were downregulated(P0.05)after interfering ***,the change of UBE2I expression was associated with the level of estradiol and its receptors(AR and ESR),which suggests that UBE2I is necessary to initiate the female-specific development in *** conclusion,this work demonstrates that UBE2I is a crucial sex differentiation-related gene in the embryonic development of chickens,which provides insights for further understanding the mechanism of sex determination in chickens.