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PROTEIN S-ACYl TRANSFERASE 13/16 modulate disease resistance by S-acylation of the nucleotide binding,l.ucine-rich repeat protein R5l1 in Arabidopsis

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Journal of Integrative Plant Biology 2022年第9期64卷 1789-1802页

作者： Jin Gao Gai Huang xin Chen yu-Xian Zhu State Key laboratory of Protein and Plant Gene Research College of Life SciencesPeking UniversityBeijing100871 China Institute for Advanced Studies Wuhan UniversityWuhan430072 China

Nucleotide binding,l.ucine-rich repeat(NB-lRR)proteins are critical for disease resistance in plants,while we do not know whether S-acylation of these proteins plays a role during bacterial *** identified 30 Arabidopsis mutants with mutations in NB-lRR encoding genes from the Nottingham Arabidopsis Stock Center and characterized their contribution to the plant immune response after inoculation with Pseudomonas syringae pv tomato DC3000(Pst DC3000).Of the five mutants that were hyper-susceptible to the pathogen,three(R5l1,R5l2 and RPS5)proteins contain the conserved S-acylation site in the N-terminal coiled-coil(CC)*** wild-type(WT)Arabidopsis plants,R5l1 was transcriptionally activated upon pathogen infection,and R5l1 overexpression lines had enhanced *** experiments indicated that R5l1 localized at the plasma membrane(PM)via S-acylation of its N-terminal CC domain,which was mediated by PROTEIN S-ACYl TRANSFERASE 13/16(PAT13,PAT16).Modification of the S-acylation site reduced its affinity for binding the PM,with a consequent significant reduction in bacterial *** localization of R5l1 was significantly reduced in pat13 and pat16 mutants,similar to what was found for WT plants treated with 2-bromopalmitate,an S-acylationblocking *** plants expressing R5l1 in the pat13 pat16 double mutant showed no enhanced disease *** of R5l1 in WT Arabidopsis resulted in substantial accumulation of reactive oxygen species after inoculation with Pst DC3000;this effect was not observed with a mutant R5l1 carrying a mutated Sacylation *** data suggest that PAT13-and PAT16-mediated S-acylation of R5l1 is crucial for its membrane localization to activate the plant defense response.

关键词： NB-lRR protein S-acyl transferases(PATs) Pst DC3000 S-acylation

A haemorrhagic cholecystitis presenting as obstructive jaundice

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Hepatobiliary Surgery and Nutrition 2021年第2期10卷 299-300页

作者： xin Chen liangliang yu Department of Gastroenterology Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhouChina

A 63-year-old female suffered right upper quadrant pain for 5 days,and icteric sclera for 3 *** laboratory examinations revealed a normal blood routine and abnormal bilirubin levels:total bilirubin 222.0μmol/l,direct bilirubin 131.8μmol/*** occult blood was ***/pelvis CT(Panel A)and MRCP(Panel B)showed cholecystolithiasis and lower choledocholithiasis.

关键词： routine cholecystitis jaundice

Forward Expansiveness and Entropies for Subsystems of Z^(k)_(+)-actions

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Acta Mathematica Sinica,English Series 2023年第4期39卷 633-662页

作者： Yao Jia GuO xin Sheng WANG yu Jun ZHu School of Mathematical Sciences Xiamen UniversityXiamen 361005P.R.China

In this paper,forward expansiveness and entropies of"subsystems"2)of Z^(k)_(+)-actions are ***αbe a Z^(k)_(+)-action on a compact metric *** each 1≤j≤k-1,denote G^(j)_(+)={V+:=V∩R^(k)_(+):V is a j-dimensional subspace of R^(k)}.We consider the forward expansiveness and entropies forαalong V+∈G^(j)_(+).Adapting the technique of"coding",which was introduced by *** and *** to investigate expansive subdynamics of Z^(k)-actions,to the Z^(k)_(+)cases,we show that the set E^(j)_(+)(α)of forward expansive j-dimensional V_(+)is open in G^(j)_(+).The topological entropy and measure-theoretic entropy of j-dimensional subsystems ofαare both continuous in E^(j)_(+)(α),and moreover,a variational principle relating them is *** a 1-dimensional ray l∈G^(+)_(1),we relate the 1-dimensional subsystem ofαalong l to an *** *** the techniques of random dynamical systems we investigate the entropy theory of 1-dimensional *** particular,we propose the notion of preimage entropy(including topological and measure-theoretical versions)via the preimage structure ofαalong *** show that the preimage entropy coincides with the classical entropy along any l∈E1+(α)for topological and measure-theoretical versions ***,a formula relating the measure-theoretical directional preimage entropy and the folding entropy of the generators is obtained.

关键词： Z^(k)_(+)-action forward expansiveness j-dimensional subsystems entropy preimage entropy folding entropy variational principle random transformation

Simultaneous biodegradation of nitrogen-containing aromatic compounds in a sequencing batch bioreactor

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Journal of Environmental Sciences 2007年第5期19卷 530-535页

作者： l.u xing-yu WANG Bao-jun JIANG Cheng-ying ZHAO Ke-xin Harold l. Drake l.u Shuang-Jiang State Key lab of Microbial Resources at Institute of Microbiology Chinese Academy of Sciences Beijing 100080 China Graduate university of Chinese Academy of Sciences Beijing 100049 China Department of Ecological Microbiology University of Bayreuth 95445 Bayreuth Germany

Many nitrogen-containing aromatic compounds （NACs）, such as nitrobenzene （NB）, 4-nitrophenol （4-NP）, aniline （AN）, and 2,4-dinitrophenol （2,4-DNP）, are environmentally hazardous, and their removal from contaminated water is one of the main challenges facing wastewater treatment plants. In this study, synthetic wastewater containing NB, 4-NP, 2,4-DNP, and AN at concentrations ranging from 50 to 180 mg/l was fed into a sequencing batch reactor （SBR）. Analyses of the SBR system indicated that it simultaneously removed more than 99% of the NACs at loading rates of 0.36 kg NB/（m^3·d）, 0.3 kg 4-NP/（m^3·d）, 0.25 kg AN/（m^3·d）, and 0.1 kg 2,4-DNP/（m^3·d）. Bacterial groups of Bacteriodetes, Candidate division TM7, α-Proteobacteria, and β-Proteobacteria were dominant in the clone libraries of 16S rRNA genes retrieved from the microbial communities in the SBR system. ＂Cycle tests＂ designed to alter feeding and aeration parameters of the SBR system demonstrated that the resident microbial biome of the SBR system responded rapidly to changing conditions. Consumption of O2 was concomitant with the apparent mineralization of NACs. Aromatic ring-cleaving dioxygenase activities suggested that （1） AN and NB were degraded via catechol 2,3-dioxygenase; （2） 4-NP was degraded via 1,2,4-benzentriol 1,2-dioxygenase; and （3） 2,4-DNP was degraded via an unresolved pathway.

关键词： nitrogen-containing aromatic compounds biodegradation sequencing batch reactor Candidate division TM7

large-scale carambola-like V_(2)O_(5) nanoflowers arrays on microporous reed carbon as improved electrochemical performances lithium-ion batteries cathode

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Journal of Energy Chemistry 2020年第12期29卷 388-395页

作者： Hua Tan xin Zhi yu Kai Huang Jianxin Zhong Bingan lu School of Physics and Optoelectronics Hunan Institute of Advanced Sensing and Information TechnologyXiangtan UniversityXiangtan 41U05HunanChina School of Physics and Electronics State Key Laboratory of Advanced Design and Manufacturing for Vehicle BodyHunan Provincial Key Laboratory of Multi-electron based Energy Storage DevicesHunan UniversityChangsha 410083HunanChina

To search for new cathode materials with high energy density of lithium-ion batteries(lIBs) is one of the most challenging issues. Vanadium pentoxide(V2 O5) with high theoretical specific capacity is believed to be a promising candidate for the next generation cathode materials, yet still suffers from low lithium ion diffusion coefficient and poor electronic conductivity resulting in low cycling life and poor rate performances. Here, we report new large-scale carambola-like V2 O5 nanoflowers arrays anchored on microporous reed carbon as high performances lIBs cathode. Each individual pore space of the microporous reed carbon is like a hexagonal cylinder, and the area of each carbon wall is more than103 um2, which is favorable for the growth of V2 O5 nanostructure arrays. After hydrothermal, the largescale carambola-like V2 O5 nanoflowers arrays can directly grow on the surface of microporous *** to the novel composite structures, the V2 O5 nanoflowers arrays@microporous carbon stabilizes at273 mA h g^(-1) after 100 cycles at 0.2 C. When cycling at 1.0 C over 500 cycles, the capacity still maintains at 180 mAh g^(-1). The demonstrated approach in this work paves the way for the development of high rate capability and excellent cycling stability V2 O5-based cathode materials.

关键词： V_(2)O_(5) Cathode Reed carbon Microporous lithium-ion batteries

Sulfite as the substrate of C-sulfonate metabolism ofα,β-unsaturated carbonyl containing andrographolide:analysis of sulfite in rats'intestinal tract and the reaction kinetics of andrographolide with sulfite

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Chinese Journal of Natural Medicines 2021年第9期19卷 706-712页

作者： HuO Zhi-Peng FENG xin-Chi WANG yu TIAN yu-Ting QIu Feng School of Chinese Materia Medica and State Key Laboratory of Component-based Chinese MedicineTianjin University of Traditional Chinese MedicineTianjin 301617China ~TCM Research Center Tasly AcademyTasly Holding Group Co.Ltd.Tianjin 300402China State Key laboratory of Critical Technology in Innovative Chinese Medicine Tasly Pharmaceutical Co.Ltd.Tianjin 300402China Institute of Pharmacy China Pharmaceutical UniversityNanjing 210009China Institute of Pharmaceutical Science and Technology Tianjin UniversityTianjin 300072China

One-sixth of the currently known natural products containα,β-unsaturated carbonyl *** previous studies reported a rare C-sulfonate metabolic *** groups were linked to theβ-carbon ofα,β-unsaturated carbonyl-based natural compounds through this ***,the mechanism of this type of metabolism is still not fully understood,especially whether it is formed through enzyme-mediated biotransformation or direct sulfite *** this work,the enzyme-mediated and non-enzymatic pathways were ***,the sulfite content in rat intestine was determined by lC-MS/*** results showed that the amount of sulfite in rat intestinal contents was from 41.5 to 383μg·g^(-1),whereas the amount of sulfite in rat feed was lower than the lower limit of quantitation(20μg·g^(-1)).Second,the reaction kinetics of sulfite-andrographolide reactions in phosphate buffer solutions(pH 6-8)was *** half-lives of andrographolide ranged from minutes to *** was suggested that the C-sulfonate reaction of andrographolide was very ***,the C-sulfonate metabolites of andrographolide were both detected when andrographolide and l-cysteine-S-conjugate andrographolide were incubated with the rat small intestine contents or sulfite,indicating that the sulfite amount in rat intestine contents was high enough to react with andrographolide,which assisted a significant portion of andrographolide ***,the comparison of andrographolide metabolite profiles among liver homogenate(with NADPH),liver S9(with NADPH),small intestine contents homogenate(with no NADPH),and sulfite solution incubations showed that the C-sulfonate metabolites were predominantly generated in the intestinal tract by non-enzymatic *** summary,sulfite can serve as a substrate for C-sulfonate metabolism,and these results identified non-enzymatically nucleophilic addition as the potential mechanism for C-sulfonate metabolism of compounds containingα,β-unsaturated carbonyl moie

关键词： Sulfite C-Sulfonate metabolites α,β-unsaturated carbonyl Reaction kinetics Andrographolide Non-enzymatic

Solvent extraction of u(Ⅵ) by N,N-dimethyl-N',N'-dioctyl.uccinylamide and N,N-dimethyl-N',N'-didecyl.uccinylamide in cyclohexane

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Nuclear Science and Techniques 2016年第3期27卷 68-73页

作者： yu Cui Xiao-Feng Yang Cheng Yang Ye-xin li Guo-Zhu Chen Guo-xin Sun School of Chemistry and Chemical Engineering University of Jinan

The extraction of uranyl nitrate by novel extractants of N,N-dimethyl-N',N'-dioctyl.uccinylamide(DMDOSA)and N,N-dimethyl-N',N'-didecyl.uccinylamide(DMDDSA) from aqueous nitric/nitrate solutions was *** was found both the concentration of HNO_3 and extractants had an effect on the u(Ⅵ) extraction *** extraction mechanism was established,and stoichiometry of the main extracted species was confirmed to be uO_2(NO_3)_2·2DMDOSA and uO_2(NO_3)_2·2DMDDSA,*** of the extraction reactions are *** spectral study of the u(Ⅵ) extracted species was also made.

关键词：溶剂萃取二甲基环己烷 ftir光谱硝酸铀酰化学计量比 O3浓度萃取反应

Synthesis of (s)-(-)-Benzyl-2-tert-butoxycarbonyl amino-4-iodobutyrate

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Chinese Chemical letters 2001年第9期12卷 767-768页

作者： xin Rong QIN Quan lI yu yuan XIE State Key laboratory of Drug Research Shanghai Institute of Materia MedicaShanghai Institutes for Biological SciencesChinese Academy of ScienceShanghai 200031

A convenient method to synthesize (s)-(-)-benzyl-2-tert-butoxy carbonylamino-4-iodobutyrate from l-(+)methionine 1 was reported, the enantiomeric purity of the product was established by comparison of optical rotation... 详细信息

关键词： alpha-amino acid chiral

Targeting ERK1/2-calpain 1-NF-κB signal transduction in secondary tissue damage and astrogliosis after spinal cord injury

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Frontiers in Biology 2015年第5期10卷 427-438页

作者： xin xin yu Vimala Bondada Colin Rogers Carolyn A. Meyer Chen Guang yu Spinal Cord and Brain Injury Research Center Department of Anatomy and Neurobiology University of Kentucky College of MedicineLexington KY 40536-0509 USA

Neuronal damage, glial inflammation, and astrogliosis/astroglial scar formation are major secondary injury mechanisms that are significant contributors to functional deficits after spinal cord injury （SCI）. The objectives of the study were to evaluate the distinct roles of ERK2 vs. ERK1/2 and ERK1/2-calpain 1 -NF-r,B signal transduction in the tissue damage and astrogliosis/astroglial scar formation following SCI in rats. RNAi approaches, pharmacological intervention （u0126）, Western blot analysis, immunofluorescence analysis, and histological assessment were used to target ERK1/2-calpain 1-NF-KB signal transduction pathway for neuroprotection. Histological staining analysis demonstrated that selectively reducing pERK2 using ERK2 siRNA, but not inhibition of pERK1/2 with u0126, significantly reduced lesion volume and improved total tissue sparing, white matter sparing, and gray matter sparing in spinal cord two weeks after contusive SCI. An ERK1/2-calpain 1-NF-KB signal transduction pathway was involved in the astroglial scar formation after SCI. Blockade of ERK1/2 by u0126 decreased calpain 1 expression 4 h following SCI. Selective calpain 1 reduction by lentiviral shRNA attenuated astroglial NF-κB activity and astroglial scar formation after SCI in rats. Taken together, these results demonstrate the involvement of individual ERK2 and caipain 1 signaling pathways in tissue damage and astrogliosis/astroglial scar formation in animal models of SCI. Therefore, targeting individual ERK and its downstream signal transduction of calpain 1-NF-κB may provide greater potential as novel therapeutics for minimizing tissue damage and astroglial scar formation following SCI.

关键词： calpain 1 ERK1/2 RNAi neurodegeneration astrogliosis spinal cord injury