检索结果-南通市图书馆

Antiulcerogenic Effect of Brazilian Propolis Formulation in Mice

Pharmacology & Pharmacy 2015年第12期6卷 580-588页

作者： Niraldo Paulino Lucas Amorim coutinho jose reinaldo coutinho Giselle Cunha Vilela Veroneide Pio da Silva Leandro Amarilis Scremin Paulino Universidade Anhanguera de Sao Paulo Sao PauloBrasil Medical Lex Gestao de Informacoes & Cursos Ltda FlorianopolisBrasil

Propolis is a natural resin used around the world to treatment of several diseases. We evaluated the effect of Brazilian propolis (P1) in guinea pig isolated trachea in vitro contracted with hista-mine, in paw oedema induced by histamine in mice and anti-ulcerogenic effect of propolis formulation in mice. We used a P1 extract from South of Brazil to produce a formulation containing sodium bicarbonate and propolis dry extract (2%). In the guinea pig trachea Brazilian propolis extract (10 μg/mL) produced a non-significant inhibition in the cumulative response curve for histamine. However, the dose of 100 μg/mL shown had a significant inhibitory effect on the contraction induced by histamine (51% ± 5%). In the mouse paw oedema induced by intraplantar injection of histamine (50 μg/paw), the results showed that the treatment of the mice with P1 (10 or 100 mg/Kg (p.o) or 1 or 10 mg/Kg (i.p)), 0.5 h beforehand, significantly inhibited the paw oedema with maximal inhibition (MI) of 25% or 42% by p.o. route or 22% or 37% by i.p. route, respectively. Finally we have pre-treated mice with P1 formulation (20 mg/Kg or 40 mg/Kg, p.o, one time) or vehicle (powder) in animals submitted to diclofenac-induced ulcer, after 8 h P1 formulation inhibited of 43% or 83% of ulcers scores, respectively. Results showed that P1 formulation inhibited the ulcerogenic effect induced by diclofenac. Results suggested also, at least in part, the antiulcerogenic effect of formulation containing standardized extract of propolis can be due to the antagonistic action on the histaminergic system.

关键词： Propolis Formulation Ulcer Stomach Mice Palavras Chaves: Própolis Formula??o Oral úlcera Est?mago Camundongo

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Analysing vaccine efficacy evaluated in phase 3 clinical trials carried out during outbreaks

引用

Infectious Disease Modelling 2024年第4期9卷 1027-1044页

作者： Francisco Antonio Bezerra coutinho Marcos Amaku Fernanda Castro Boulos jose Alfredo de Sousa Moreira Joao Italo DiasFranca Julio Antonio do Amaral Eliana Nogueira Castro de Barros Claudio jose Struchiner Esper Jorge Kallas Eduardo Massad School of Medicine University of Sao PauloBrazil Instituto Butantan Sao PauloBrazil School of Applied Mathematics Fundacao Getulio VargasRio de JaneiroBrazil

In this paper we examine several definitions of vaccine efficacy(VE)that we found in the literature,for diseases that express themselves in outbreaks,that is,when the force of infection grows in time,reaches a maximum and then *** fact that the disease occurs in outbreaks results in several problems that we *** propose a mathematical model that allows the calculation of VE for several *** trials usually needs a large number of volunteers that must be ***,all volunteers should be enrolled in approximately the same time,but this is generally impossible for logistic reasons and they are enrolled in a fashion that can be replaced by a continuous density function(for example,a Gaussian function).The outbreak can also be replaced by a continuous density function,and the use of these density functions simplifies the ***,for example Gaussian functions,one of the problems one can immediately notice is that the peak of the two curves do not occur at the same *** model allows us to conclude:First,the calculated vaccine efficacy decreases when the force of infection increases;Second,the calculated vaccine efficacy decreases when the gap between the peak in the force of infection and the peak in the enrollment rate increases;Third,different trial protocols can be simulated with this model;different vaccine efficacy definitions can be calculated and in our simulations,all result are approximately the *** final,and perhaps most important conclusion of our model,is that vaccine efficacy calculated during outbreaks must be carefully examined and the best way we can suggest to overcome this problem is to stratify the enrolled volunteer's in a cohort-by-cohort basis and do the survival analysis for each cohort,or apply the Cox proportional hazards model for each cohort.

关键词： Vaccine efficacy Clinical trials Mathematical models Simulations Covid-19

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa(Jacq.) Raf.(Cleomaceae)

引用

Asian Pacific Journal of Tropical Biomedicine 2019年第1期9卷 12-17页

作者： Francisca Samara Muniz dos Santos jose Weverton Almeida Bezerra Jean Paul Kamdem Aline Augusti Boligon Marli Matiko Anraku Ana Raquel Pereira da Silva Kleber Ribeiro Fidelis Nadghia Figueiredo Leite Antonio Ivanildo Pinho Henrique Douglas Melo coutinho jose Edilson Goncalves dos Santos Microscopy Laboratory Regional University of Cariri (URCA) Program Post graduate in Plant Biology (UFPE) Phytochemical Laboratory Department of Industrial Pharmacy Federal University of Santa Maria (UFSM) Microbiology and Molecular Biology Laboratory Regional University of Cariri (URCA)

Objective: To evaluate the antibacterial activity and neuroprotective capacity of the ethanolic and aqueous extracts of Tarenaya spinosa(T. spinosa) as well as to determine and quantify some of its polyphenols by high performance liquid chromatography with diode-array detection(HPLC-DAD). Methods: The bacterial Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa strains, grown in Heart Agar Infusion, were tested. The drugs gentamicin, norfloxacin and imipenem were used to evaluate the modulating or antagonistic capacity of the T. spinosa extracts. The extract was analysed by HPLC-DAD to determine the main phenolic compounds. For the cell viability tests, individual heads of the Nauphoeta cinerea arthropod model were removed, homogenized in Trifluoromethyl ketone and centrifuged afterwards. Subsequently, 20 μL of NaN O2 were added to the biological material, except in the control group, to evaluate the protection capacity of the extracts. The homogenate of the insect heads was incubated for 2 h in tubes containing tetrazolium bromide. Results: HPLC-DAD demonstrated that the ethanolic extract of T. spinosa presented caffeic acid as the major compound. The ethanolic extract also showed neuroprotective effects at concentrations ≥ 10 μg/mL, while aqueous extract was shown to have a protective effect only at the concentration of 100 μg/mL. The aqueous extract demonstrated a clinically relevant antibacterial activity against the Staphylococcus aureus multidrug resistant strain-MDR, with MIC 512 μg/m L. However, when the extracts were associated with gentamicin and imipenem, a synergism was detected against Staphylococcus aureus and Escherichia coli MDR strains. Conclusions: Although it does not present an antibacterial action, the extracts of T. spinosa can be used in the pharmaceutical industries since its extracts show modulating action of drugs. Besides, these natural products have neuroprotective capacity.

关键词： Tarenaya spinosa Cleome spinose Nauphoeta cinerea Synergism Antibiotic resistance

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Peroral endoscopic myotomy vs laparoscopic myotomy and partial fundoplication for esophageal achalasia: A single-center randomized controlled trial

引用

World Journal of Gastroenterology 2022年第33期28卷 4875-4889页

作者： Eduardo Turiani Hourneaux de Moura jose Jukemura Igor Braga Ribeiro Galileu Ferreira Ayala Farias Aureo Augusto de Almeida Delgado Lara Meireles Azeredo coutinho Diogo Turiani Hourneaux de Moura Rubens Antonio Aissar Sallum Ary Nasi Sergio A Sanchez-Luna Paulo Sakai Eduardo Guimaraes Hourneaux de Moura Departamento de Gastroenterologia Faculdade de MedicinaUniversidade de Sao PauloServico de Endoscopia Gastrointestinal do Hospital das Clinicas HCFMUSPSao Paulo 05403-010Brazil Division of Esophageal Surgery Hospital das ClinicasUniversity of Sao Paulo School of MedicineSao Paulo 05403-010Brazil Departamento de Gastroenterologia Faculdade de MedicinaUniversidade de Sao PauloHospital das ClinicasUniversity of Sao Paulo School of MedicineSao Paulo 05403-010Brazil Department of Internal Medicine The University of Alabama at Birmingham Heersink School of MedicineBasil I.Hirschowitz Endoscopic Center of ExcellenceDivision of Gastroenterology and HepatologyBirminghamAL 35233United States

BACKGROUND Achalasia is a rare benign esophageal motor disorder characterized by incomplete relaxation of the lower esophageal sphincter(LES). The treatment of achalasia is not curative, but rather is aimed at reducing LES pressure. In patients who have failed noninvasive therapy, surgery should be considered. Myotomy with partial fundoplication has been considered the first-line treatment for non-advanced achalasia. Recently, peroral endoscopic myotomy(POEM), a technique that employs the principles of submucosal endoscopy to perform the equivalent of a surgical myotomy,has emerged as a promising minimally invasive technique for the management of this *** To compare POEM and laparoscopic myotomy and partial fundoplication(LM-PF) regarding their efficacy and outcomes for the treatment of *** Forty treatment-naive adult patients who had been diagnosed with achalasia based on clinical and manometric criteria(dysphagia score ≥ II and Eckardt score > 3) were randomized to undergo either LM-PF or POEM. The outcome measures were anesthesia time, procedure time, symptom improvement, reflux esophagitis(as determined with the Gastroesophageal Reflux Disease Questionnaire), barium column height at 1 and 5 min(on a barium esophagogram), pressure at the LES, the occurrence of adverse events(AEs), length of stay(LOS), and quality of life(QoL).RESULTS There were no statistically significant differences between the LM-PF and POEM groups regarding symptom improvement at 1, 6, and 12 mo of follow-up(P = 0.192, P = 0.242, and P = 0.242, respectively). However, the rates of reflux esophagitis at 1, 6, and 12 mo of follow-up were significantly higher in the POEM group(P = 0.014, P < 0.001, and P = 0.002, respectively). There were also no statistical differences regarding the manometry values, the occurrence of AEs, or LOS. Anesthesia time and procedure time were significantly shorter in the POEM group than in the LM-PF group(185.00 ± 56.89 and 95.70 ± 30.47 min v

关键词： Esophageal achalasia Gastroesophageal reflux Deglutition disorders Heller myotomy Fundoplication Randomized controlled trial

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

An Alternative Manifold for Cosmology Using Seifert Fibered and Hyperbolic Spaces

引用

Applied Mathematics 2014年第6期5卷 1013-1028页

作者： Maria E.Mejía reinaldo R.Rosa Departamento de Matemáticas Universidad de GuadalajaraGuadalajaraMéxico Laboratório Associado de Computacao e Matemática Aplicada Instituto Nacional de Pesquisas EspaciaisSao Jose dos CamposBrasil

We propose a model with 3-dimensional spatial sections, constructed from hyperbolic cusp space glued to Seifert manifolds which are in this case homology spheres. The topological part of this research is based on Thurston’s conjecture which states that any 3-dimensional manifold has a canonical decomposition into parts, each of which has a particular geometric structure. In our case, each part is either a Seifert fibered or a cusp hyperbolic space. In our construction we remove tubular neighbourhoods of singular orbits in areas of Seifert fibered manifolds using a splice operation and replace each with a cusp hyperbolic space. We thus achieve elimination of all singularities, which appear in the standard-like cosmological models, replacing them by “a torus to infinity”. From this construction, we propose an alternative manifold for cosmology with finite volume and without Friedmann-like singularities. This manifold was used for calculating coupling constants. Obtaining in this way a theoretical explanation for fundamental forces is at least in the sense of the hierarchy.

关键词： Topology Cosmology Thurston’s Theory Singularity-Free

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Central depressant effects of Piper tuberculatum Jacq essential oil in mice

引用

Food Bioscience 2022年第4期48卷 620-629页

作者： Valterlúcio dos Santos Sales Francisco Romero Cabral Emmily Petícia do Nascimento Sales Tatyelle Bezerra Carvalho Maria Haiele Nogueira Costa Victor Afonso Pereira de Oliveira Cristina Kelly de Souza Rodrigues Francisco Rodolpho Sobreira Dantas Nobrega de Figueiredo Daniel Souza Bezerra Gyllyandeson de Araújo Delmondes Henrique Douglas Melo coutinho jose Galberto Martins da Costa Irwin Rose Alencar de Menezes Cícero Francisco Bezerra Felipe Marta Regina Kerntopf Universidade Regional do Cariri Departamento de Química Biol´ogicaCratoCear´aBrazil Universidade Federal de S˜ao Paulo-Departamento de Fisiologia S˜ao PauloSPBrazil Faculdade de Ciˆencias M´edicas da Santa Casa de S˜ao Paulo S˜ao PauloSPBrazil Universidade Federal da Paraíba Departamento de Biologia MolecularJo˜ao PessoaParaíbaBrazil

This study aims to investigate the toxicity and possible effects and mechanism of action of the essential oil obtained from the Piper tuberculatum Jacq.‘s fruit(EOPT)on the central nervous system(CNS)of *** the Hippocratic test,it was observed ambulation reduction,sedation,piloerection and mortality of 25%at the dose of 1000 mg/kg and 50%at the dose of 5000 mg/*** results found demonstrate that the EOPT is endowed with a possible depressant effect,and is characterized by a likely sedative(open field test),anxiolytic-like(elevated plus maze test),anticonvulsant(pentylenetetrazole-induced seizure test)and hypnotic action(pentobarbital-induced sleeping time).These effects may possibly occur due to the interaction between the monoterpenes,present in its essential oil,and the GABAergic pathway confirmed by reversion of the effects by ***,these results demonstrate the therapeutic potential and the validation of the ethnopharmacological use of this species in the treatment of disorders affecting the central nervous system.

关键词： Piper tuberculatum Jacq. Essential oil Sedative Anxiolytic Hypnotic

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Challenges in Implementing DVI Best Practice Guidelines Due to the Brumadinho Disaster

引用

Forensic Medicine and Anatomy Research 2024年第1期12卷 1-12页

作者： reinaldo Moreira Silva Bruno Diniz Sander Morais Carlos Danilo Ruas Silva Elza Cristina Lopes de Oliveira Fernando Pereira Macedo Giovanni Vitral Pinto Guadalupe Dell’Arret coutinho Higgor Gonçalves Dornelas João Luiz Silva Moreira Laila Evangelista de Almeida Fernandes Marcia Lobão Iannini Patrícia Siqueira Valéria Rosalina Dias e Santos Sordaini Maria Caligiorne Instituto de Criminalística Polícia Civil de Minas Gerais Belo Horizonte Brazil

The broke of a tailings dam from Vale S.A. destroyed a gigantic area killing 270 persons in Brumadinho/Brazil. Organizing activities after a mass disaster is a complex process that requires the involvement of many people and resources in the laboratory. To DNA identification of the victims, a daunting work had to be made by DNA laboratory staff in a collaborative effort with many partners. Efforts to implement good practice guidelines in Disaster Victim Identification (DVI) have revealed several important aspects that need to change in the forensic DNA laboratory. This article highlights the challenges of implementing DVI best practice guidelines in resource-poor settings, but with professionals from different sectors engaged in the same goal of briefly identifying victims and helping families and society.

关键词： Disaster Victim Identification DNA Brumadinho

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cell Viability of Byrsonima intermedia A Juss Calli

引用

Journal of Agricultural Science and Technology(B) 2012年第6期2卷 713-720页

作者： Luciano coutinho Silva Renato Paiva Daiane Peixoto Vargas Diogo Pedrosa Correa da Silva Rairys Cravo Herrera Sandro Barbosa Antonio Paulino da Costa Netto Biology Department Plant Physiology Sector Federal University of Lavras (UFLA) Caixa Postal 3037 CEP 37200-000 LavrasMinas Gerais Brasil Embrapa Clima Temperado Rodovia BR 392 km 78 CP 403 Pelotas Rio Grande do Sul CEP: 96010-971 Brasil Universidade Federal do Pare (UFPA) Campus Universitfrio de Altamira Rua Coronel Jose Porfirio 2515 Sao Sebastiao CEP68372-040 Altamira Pard Brasil Departamento de Ciencias Biologicas e da Terra Universidade Federal de Alfenas (UNIFAL) MG Rua Gabriel Monteiro da Silva 700 Centro CEP 37130-000 Alfenas Minas Gerais Brasil Laboratorio de Fisiologia Vegetal Universidade Federal de Goifs (UFG) Rua Riaehuelo S/N Unidade Jatoba Setor SamuelGraham 75804-020 Jatai GO Brasil

Byrsonima intermedia A Juss. is a species with pharmacological properties from the Brazilian Cerrado that shows difficulties related to sexual propagation. Research on cell viability may provide useful information for the selection of cells with embryogenic potential during the callus culture, Within this context, our research is aimed at establishing the cell viability of calli from Byrsonima intermedia leaf segments. The calli went through three subculture phases, of 60 days each, in MS medium with 0.09 M sucrose, 0.6% agar, pH 5.8 and 4.52 laM 2,4-D. The calli were stored in dark conditions and samples were collected every 10 days from each subculture for viability tests with fluorescein 3,6-diacetate （FDA） and 2,3,5-triphenyltetrazolium chloride （TTC）. The staining methods allowed quantifying cell viability in each subculture. The best results from the FDA tests were obtained at 21, 25 and 29 days for the first, second and third subcultures respectively, with 53,86%, 61.88% and 53.73% viable cells. Regarding the TTC test, the largest absorbance values were obtained at 21, 27 and 28 days for the first, second and third subcultures respectively. Fluorescence and spectrophotometry analyses were efficient for determination of cell viability during callus cultivation period.

关键词： Cell viability fluorescein 3 6-diacetate 2 3 5-tripheniltetrazolium chloride subculture tissue culture native plant.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Brazilian contribution to a better understanding of geriatric cardiology

引用

Journal of Geriatric Cardiology 2012年第2期9卷 77-77页

作者： jose C. Nicolau Clatudia F. Gravina Roberto A. Franken Aureliano coutinho 355-14 Andar S6o Paulo 01224-020 Brazil

Brazil is a South America country with a population of approximately 20 million people. It is the 5th biggest country in the world in territory extension, and its economy ranges between the 6th and 7th globally, according to different rankings （www. ***; access on May 19, 2012）. It is a very friendly country with a huge cultural, ethnic, regional and economic heterogeneity, and is a proud member of the BRICS Group, along with Russia, India, China and South Africa.

关键词：心血管病巴西老年经济发展多样性俄罗斯

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：