Cell Viability of Byrsonima intermedia A Juss Calli

作     者：Luciano Coutinho Silva Renato Paiva Daiane Peixoto Vargas Diogo Pedrosa Correa da Silva Rairys Cravo Herrera Sandro Barbosa Antonio Paulino da Costa Netto 

作者机构：Biology Department Plant Physiology Sector Federal University of Lavras (UFLA) Caixa Postal 3037 CEP 37200-000 LavrasMinas Gerais Brasil Embrapa Clima Temperado Rodovia BR 392 km 78 CP 403 Pelotas Rio Grande do Sul CEP: 96010-971 Brasil Universidade Federal do Pare (UFPA) Campus Universitfrio de Altamira Rua Coronel Jose Porfirio 2515 Sao Sebastiao CEP68372-040 Altamira Pard Brasil Departamento de Ciencias Biologicas e da Terra Universidade Federal de Alfenas (UNIFAL) MG Rua Gabriel Monteiro da Silva 700 Centro CEP 37130-000 Alfenas Minas Gerais Brasil Laboratorio de Fisiologia Vegetal Universidade Federal de Goifs (UFG) Rua Riaehuelo S/N Unidade Jatoba Setor SamuelGraham 75804-020 Jatai GO Brasil 

出 版 物：《Journal of Agricultural Science and Technology(B)》 (农业科学与技术（B）)

年 卷 期：2012年第2卷第6期

页      面：713-720页

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

主　　题：Cell viability fluorescein 3 6-diacetate 2 3 5-tripheniltetrazolium chloride subculture tissue culture native plant. 

摘      要：Byrsonima intermedia A Juss. is a species with pharmacological properties from the Brazilian Cerrado that shows difficulties related to sexual propagation. Research on cell viability may provide useful information for the selection of cells with embryogenic potential during the callus culture, Within this context, our research is aimed at establishing the cell viability of calli from Byrsonima intermedia leaf segments. The calli went through three subculture phases, of 60 days each, in MS medium with 0.09 M sucrose, 0.6% agar, pH 5.8 and 4.52 laM 2,4-D. The calli were stored in dark conditions and samples were collected every 10 days from each subculture for viability tests with fluorescein 3,6-diacetate （FDA） and 2,3,5-triphenyltetrazolium chloride （TTC）. The staining methods allowed quantifying cell viability in each subculture. The best results from the FDA tests were obtained at 21, 25 and 29 days for the first, second and third subcultures respectively, with 53,86%, 61.88% and 53.73% viable cells. Regarding the TTC test, the largest absorbance values were obtained at 21, 27 and 28 days for the first, second and third subcultures respectively. Fluorescence and spectrophotometry analyses were efficient for determination of cell viability during callus cultivation period.