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Long-term in-vitro culture and subculture of the hemocytes of swimming crab Portunus trituberculatus

海洋湖沼学报(英文) 2023年第5期41卷 1918-1939页

作者： Liwen GUO Yaqi ZHAO Huarong GUO Key Laboratory of Marine Genetics and Breeding Ministry of EducationCollege of Marine Life SciencesOcean University of ChinaQingdao 266003China Institute of Evolution&Marine Biodiversity Ocean University of ChinaQingdao 266003China

Crab cell line,especially continuous crab cell line,can provide us a useful tool for studies on the virology,immunology,and molecular biology of ***,no continuous crab cell line has been available due to the lacking of suitable medium and the occurrence of *** this study,long-term in vitro culture conditions for both two-(2D)and three-dimensions(3D)were successfully developed for the circulating hemocytes of swimming crab Portunus trituberculatus,designated as PTH *** 2D culture,a novel crab basic medium in osmolarity of 990–1100 mOsm/kg was optimized for the first time,which is different from Leibovitz's L-15 medium in mainly the components of amino acids,containing double strengths of the contents of free amino acid mixture in the crab *** an optimal crab growth medium was developed by supplementing 5%fetal bovine serum,50-g/L yeast extract powder,20-μg/L basic fibroblast growth factor and epidermal growth factor into the optimal crab basic medium,and found that it could support a long-term survival of PTH cells in a healthy monolayer up to 347 days and partially break through the mitosis-arrest of crab cells evidenced by the obvious increase of proliferating potential detected in the 10-d primarily cultured PTH *** 2D cultured PTH cells could be successfully sub-cultured for 11 times by physical flushing method and well cryopreserved in liquid *** 3D culture,using the same crab growth medium,the PTH cell aggregates could be easily formed and healthily maintained on the surface of solidified Matrigel or in the ultra-low-attachment plate with a survival rate of 50%–60%on Day *** work largely improved the primary culture and subculture of crab cells and will facilitate the establishment of continuous crab cell line.

关键词： crab Portunus trituberculatus hemocyte long-term cell culture subculture 3D culture

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A Study of the subculture of Human Endothelial Cell From Umbilical Vein

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中国医科大学学报 1991年第S2期20卷 10-14页

作者：董玉兰陈铁镇王铁吉王跃忠张亚佳 Laboratory of Experimental Pathology China Medical University Shenyang China

Human endothelial cells derived from umbilical vein could besubcultured and survive 25 passage (double time 20～25 hours, 60～75 cumula-tive population doublings). An observation was performed with light mi-croscopy, electron microscopy and immunofluorescence microscopy using specificantiserum against factor Ⅷrelated antigen. It identified that the cultured cellswere endothelial cells. Medium RPMI-1640 supplemented with 20% humanserum, endothelial cell growth factor 200μg/ml, heparin 100μg/ml and gelatincoated flasks were very important conditions for long-term culture of humanendothelial cell.

关键词： human endothelial cell subculture endothelial cell growt factor human serum

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The Analysis on the Influence of Amarican subculture upon American College Slang

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海外英语 2013年第23期 228-229页

作者：潘丽欣南开大学外国语学院天津300071

The thesis is just trying to analyze American college slang from sub-cultural perspective on the basis of combining linguistic knowledge,the knowledge of American slang and American college slang with the knowledge of... 详细信息

关键词： subculture American college slang cultural context

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An Exploratory Study on subculture and Various Forms

An Exploratory Study on Subculture and Various Forms

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作者：何平北京物资学院外语系

subculture known as collective cultural or sub-culture,refers to those the non-mainstream, local cultural phenomenon corresponding to mainstream culture,the specific ideas and way of life belong to a region or a collective under the primary culture or a cultural *** subculture owns great significance for understanding the social structure in depth and social life.

关键词： subculture Beat generation Youth subculture Homosexuality

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Youth’s Attitude Formation in Youth Popular Music1 subculture in China

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Psychology Research 2022年第8期12卷 583-591页

作者： LI Jia Shanxi Normal University TaiyuanChina

This study was intended to analyze the youth’s attitude formation in youth popular music subculture of youth in *** of the most universal and mysterious human activities is *** Western industrialized nations,systematic instruction in harmony,counterpoint,rhythmic structure,composition,improvisation,and ear training is used to enhance the skill of *** course provides professional musicians with a variety of useful abilities that they may apply in their day-to-day *** are various disciplines that are necessary to understand how music impacts human life,from cultural studies to political economics to sociology to psychology to *** study will use descriptive regressional method as its design and *** research describes the phenomenon,condition,and *** genres of youth popular music were found to be preferred by Chinese youth in their everyday lives,as well as the link between their idols and their musical education and their views about learning youth popular music in school.

关键词： youth popular music subculture youth attitude C-Pop

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文化中的亚文化和反文化亚文化(subculture)

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现代外国哲学社会科学文摘 1984年第3期 53-54页

作者：冯凭

任何群体,凡在某些方面与社会主导性文化的价值体系不同者,都被称为亚文化。亚文化产生的原因很多。从事不同职业的人形成不同的职业亚群体,各社会阶级常形成各自的亚世界,宗教群体有时拥有与主导性文化不同的价值体系。从某种意义上讲... 详细信息

任何群体,凡在某些方面与社会主导性文化的价值体系不同者,都被称为亚文化。亚文化产生的原因很多。从事不同职业的人形成不同的职业亚群体,各社会阶级常形成各自的亚世界,宗教群体有时拥有与主导性文化不同的价值体系。从某种意义上讲,这些群体各具有自己的价值观,而这些价值观则散布在种种主导性文化信仰之间。它们并不否定主导性文化,仅仅是忽视其某

关键词：亚文化价值体系主导性文化 subculture

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饭圈文化下青少年网络追星失范现象研究

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新闻传播科学 2024年第3期12卷 824-828页

作者：何家欣北京印刷学院新闻传播学院，北京

“饭圈文化”是一种亚文化在泛娱乐主义在文化领域的具化，其是由“粉丝”的需求而生成的。青少年参与饭圈活动并积极互动，俨然成为了数字时代下的普遍现象。而青少年群体还未形成正确的价值观，在追星的过程中容易受到群体非理性、偶... 详细信息

“饭圈文化”是一种亚文化在泛娱乐主义在文化领域的具化，其是由“粉丝”的需求而生成的。青少年参与饭圈活动并积极互动，俨然成为了数字时代下的普遍现象。而青少年群体还未形成正确的价值观，在追星的过程中容易受到群体非理性、偶像魅粉以及资本逻辑的影响，从而在网络上做出一些失范的行为。为此，可以通过政府管理、提升青少年自身媒介素养、偶像自身等角度入手，正向引导青少年，从而解决青少年网络追星的失范现象。

关键词：饭圈文化青少年亚文化 Fandom Circle Culture Youth subculture

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当代中国青年亚文化总体特征与建构路径探究

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文化研究 2024年第2期

作者：张亮

当代中国青年形成了具有中国特色的青年亚文化。该文化形态在中国式现代化进程中逐渐生成，并在全球化与网络化时代影响下实现亚文化构建，呈现出边缘化、风格化、抵抗性与符号化等基本特征。国内学界对该文化形态的认识存在研究模式、... 详细信息

当代中国青年形成了具有中国特色的青年亚文化。该文化形态在中国式现代化进程中逐渐生成，并在全球化与网络化时代影响下实现亚文化构建，呈现出边缘化、风格化、抵抗性与符号化等基本特征。国内学界对该文化形态的认识存在研究模式、话语体系、科学评价标准、具体发展思路不成熟等问题。全社会须在整体、客观、历史、理性评价中国青年亚文化基础上，为中国青年亚文化提供全面引导，使中国青年早日成为社会主义的合格建设者和可靠接班人。

关键词：中国青年亚文化总体特征建构路径 Chinese youth subculture general characteristics construction path

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Research on the Multiple Exclusion Phenomenon in the Chinese“Core Weeaboo”Group

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Psychology Research 2022年第9期12卷 755-762页

作者： XIA Zhiming The Chinese University of Hong Kong(Shenzhen) ShenzhenChina

Among the subcultures that have developed rapidly in recent years,weeaboo culture covers a growing number of ***,corresponding differentiation has taken place among the weeaboos,typically including“core weeaboo”group and“pan weeaboo”***,“core weeaboos”refer to a group of people who are deeply involved in the weeaboo *** one hand,in the online community,“core weeaboos”tend to reject normal interaction with“pan weeaboos”,who they think are not deeply involved in the culture;on the other hand,due to the long-term prejudice of the mainstream culture against the weeaboos,the“core weeaboo”group has also suffered from the exclusion from the mainstream culture for a long time,thus forming a unique chain of“multiple exclusion”.With the“core weeaboo”group as the research object,this paper conducts online and field research on the phenomenon of“multiple exclusion”in the relevant groups by means of questionnaire and *** is found that the phenomenon of“multiple exclusion”in essence can be considered a two-way interaction between the“core weeaboo”group and the mainstream *** order to alleviate this phenomenon,this paper advocates that mainstream culture should increase its understanding of subcultural groups,and actively encourage high-quality“fusion”,so as to reduce the adverse effects on corresponding subcultural groups.

关键词： weeaboo culture subculture exclusion

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Direct Organogenesis from Cotyledonary Node Explants of Cucurbita pepo (L.)—An Important Zucchini Type Vegetable Crop

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American Journal of Plant Sciences 2015年第1期6卷 157-162页

作者： Muruganantham Mookkan Department of Plant Pathology and Weed Science ARO Volcani Center POB6 Bet Dagan Israel Department of Horticulture University of Georgia Tifton GA USA Present Address and Correspondence: Division of Plant Sciences University of Missouri Columbia Missouri USA

Cotyledonary node explants from 3 - 5-d-old seedling derived explants of Cucurbita pepo (L.) produced multiple shoots in Murashige and Skoog (MS) salts B5 vitamins containing medium in the presence of N6-benzylamino-purine BAP 1 mg/L + Thidiazuron TDZ 0.05 mg/L. After 4 weeks explants were subcultured to medium containing MS salts B5 Vitamins BAP 0.5 mg/L, Gibberellic acid GA3 1 mg/L and L-Glutamine 15 mg/L. Periodic excision of regenerated shoots from the explants increased shoot efficiency during subculture. The combination of L-Glutamine with BA and GA3 significantly increased the shoot proliferation. Elongated shoots were excised from shoot clumps and transferred to rooting medium containing indole-3-butyric acid (IBA, 1.0 mg/L). The rooted plants were hardened in small pot containing standardized garden soil, well developed plant transferred to greenhouse and survival rate was 85%.

关键词： Cucurbita pepo Zucchini Cotyledonary Node subculture

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