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无细胞蛋白表达系统新进展及在生物制药工程中的应用

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生物工程学报 2014年第10期30卷 1491-1503页

作者：盛嘉元张绪郑强徐志南浙江大学化学工程与生物工程学系浙江杭州310027

无细胞蛋白表达体系是一种以细胞抽提物为基础的体外合成蛋白质表达技术,具有遗传背景简单、反应操控简便等特点,已成为研究生物反应系统的重要技术手段。在研究人员的不断努力下,反应体系从原核扩展到真核蛋白质合成体系,而且目标蛋白... 详细信息

无细胞蛋白表达体系是一种以细胞抽提物为基础的体外合成蛋白质表达技术,具有遗传背景简单、反应操控简便等特点,已成为研究生物反应系统的重要技术手段。在研究人员的不断努力下,反应体系从原核扩展到真核蛋白质合成体系,而且目标蛋白表达量从毫克级提高到数克级每升,成本不断降低,反应规模可达到百公升级。近年来,无细胞蛋白表达系统在复杂蛋白、毒性蛋白和膜蛋白表达方面的优势逐渐体现,展示了其在生物制药领域的重要应用潜力。总之,无细胞技术已经成为异源蛋白质高效合成和生物制药领域中有巨大潜力的新策略。

关键词：无细胞蛋白表达系统进展生物制药

Channel selection against electrode shift enables robust myoelectric control without retraining

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Science China(Technological Sciences) 2021年第8期64卷 1653-1662页

作者： LV Bo HE jiayuan sheng XinJun DING Han ZHU XiangYang State Key Laboratory of Mechanical System and Vibration School of Mechanical EngineeringShanghai Jiao Tong UniversityShanghai200240China Department of Systems Design Engineering Faculty of EngineeringUniversity of WaterlooWaterlooN2L 3G1Canada

Myoelectric controlled interfaces driven by muscle activities have achieved good performance in ideal conditions and showed many potential medical-related and industrial ***,in practical applications,the performance could be drastically degraded due to the electrode(sensor)shift,which is inevitable in donning and doffing the *** this study,we presented a novel channel selection method against electrode shift for robust pattern-recognition based myoelectric *** proposed method was evaluated on twenty-four subjects,including twenty-two able-bodied subjects and two amputees,and compared with two traditional channel selection methods,i.e.,uniform selection(UNI)and sequential feature selection(SFS).We demonstrated that the offline error rates of the proposed method were significantly lower than those of the other two methods(P<0.05),and its online performance in shift conditions was comparable to that in ideal *** outcomes benefit the practical applications of robust myoelectric controlled interfaces.

关键词： myoelectric control high-density electrodes channel selection electrode shift surface electromyography

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煤化工合成气低温分离工艺优化与分析

石油与天然气化工 2024年第1期53卷 54-60页

作者：杨佳原盛伟郑海坤朱鉴宇李志永河南理工大学机械与动力工程学院哈密豫新能源产业研究院

目的以新疆哈密某公司煤化工工艺过程为研究对象,解决煤化工合成气低温分离液化系统高能耗问题。方法通过Aspen HYSYS软件模拟了单级混合制冷剂合成天然气分离液化流程,采用BOX算法,以系统最低能耗为目标函数,冷箱最小换热温差3℃为约... 详细信息

目的以新疆哈密某公司煤化工工艺过程为研究对象,解决煤化工合成气低温分离液化系统高能耗问题。方法通过Aspen HYSYS软件模拟了单级混合制冷剂合成天然气分离液化流程,采用BOX算法,以系统最低能耗为目标函数,冷箱最小换热温差3℃为约束条件,优化了混合制冷剂组分配比及混合制冷剂循环一、二级压缩压力。结果在保证LNG和制甲醇原料气产量不变的前提下,优化后冷箱冷热复合曲线更接近且平滑,换热效果更优,系统总能耗降低了16.59%,火用效率由37.96%提升至43.04%,显著提高了能源利用率。结论BOX算法优化混合冷剂配比及压缩压力对降低合成气液化工艺能耗、提高系统火用效率有显著效果,对煤化工合成气低温分离液化工艺的研究具有借鉴意义。

关键词：煤化工低温分离 HYSYS模拟 BOX算法火用效率

基于CARE-Ⅱ的颈动脉轻度狭窄患者易损斑块分布研究

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国际放射医学核医学杂志 2024年第4期48卷 215-223页

作者：吴曙黄娟胡佳圆常嫣格焦晟宋焱赵锡海北京医院放射科国家老年医学中心中国医学科学院老年医学研究院北京100730 清华大学医学院生物医学工程系清华大学生物医学影像研究中心北京100084

目的基于中国动脉粥样硬化风险评估第Ⅱ期(CARE-Ⅱ)研究探讨不同性别、年龄颈动脉轻度狭窄患者易损斑块的分布差异,以期为该类人群进行分层管理提供依据。方法回顾性分析2012年1月至2014年12月间CARE-Ⅱ研究数据库中双侧颈动脉狭窄率55... 详细信息

目的基于中国动脉粥样硬化风险评估第Ⅱ期(CARE-Ⅱ)研究探讨不同性别、年龄颈动脉轻度狭窄患者易损斑块的分布差异,以期为该类人群进行分层管理提供依据。方法回顾性分析2012年1月至2014年12月间CARE-Ⅱ研究数据库中双侧颈动脉狭窄率55岁),分析颈动脉斑块特征的性别差异。2组间临床资料的比较采用t检验及χ^(2)检验;2组间斑块特征的比较采用Mann-Whitney U检验及χ^(2)检验;轻度狭窄的颈动脉易损斑块相关的临床及影像因素分析采用多因素Logistic回归分析。结果男性组患者的身体质量指数[(24.50±2.87)kg/m2对(23.69±3.52)kg/m2]、舒张压[(88.63±14.68)mm Hg对(84.36±10.81)mm Hg]以及吸烟史比例[(69.2%(276/399)对7.5%(17/288))]均明显高于女性组,且差异均有统计学意义(t=−2.896、−4.163,χ^(2)=222.008,均P55岁的人群。Logistic多因素回归分析表明,年龄>55岁、狭窄程度及斑块负荷是美国心脏协会Ⅵ型斑块的独立危险因素(95%CI:1.267~7.891、1.014~1.061、1.175~1.311,均P<0.05)。结论在中国颈动脉轻度狭窄人群中,男性、高龄患者的颈动脉斑块负荷更大,易损斑块更多,可为该类人群的分层管理提供依据。

关键词：动脉粥样硬化颈动脉磁共振成像易损斑块性别

Laboratory-based Surveillance and Clinical Profile of Sporadic HEV Infection in Shanghai,China

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Virologica Sinica 2021年第4期36卷 644-654页

作者： Jie Lu Qing Li jiayuan Jiang Ziqiang Li Peiyun Wang Zike sheng Rongtao Lai Huijuan Zhou Wei Cai Hui Wang Qing Guo Honglian Gui Qing Xie Department of Infectious Diseases Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China Clinical Research Institute Shanghai Jiao Tong University School of MedicineShanghai 200025China

The study aimed to describe the epidemiological,virological and clinical features of sporadic HEV infection in eastern China.A total of 6112 patient sera were tested for anti-HEV IgG or anti-HEV IgM during one consecutive year(between August 2018 and July 2019).HEV RNA presence was evaluated by RT-PCR and HEV sequences were phylogenetically *** features of confirmed HEV-infected patients were *** sero-positivity rate of anti-HEV IgG maintained stable around 40%,while an obvious winter spike of anti-HEV IgM prevalence was observed.A total of 111 patients were confirmed of HEV viremia by molecular *** 4d was *** analyses suggest that certain strains circulate across species and around the *** with confirmed current HEV infection had a high median age(58 years)and males were predominant(62.2%).Most patients presented with jaundice(75.7%)and anorexia(68.0%).Significantly elevated levels of liver enzymes and bilirubin were ***,the baseline bilirubin level was positively correlated with illness ***-existing HBV carriage may deteriorate *** clinical burden caused by locally acquired HEV infection is *** should be enforced especially during the transition period from winter to *** with higher level of bilirubin at disease onset had slower recovery from HEV infection.

关键词： Hepatitis E virus(HEV) Seroprevalence Genotyping Epidemiology Surveillance

Efficacy of comprehensive cognitive health management for Shanghai community older adults with mild cognitive impairment

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General Psychiatry 2022年第4期35卷 240-248页

作者： jiayuan Qiu Lu Zhao Shifu Xiao Shaowei Zhang Ling Li Jing Nie Li Bai Shixing Qian Yang Yang Michael Phillips Meiqing sheng Yuan Fang Xia Li Department of Geriatric Psychiatry Shanghai Mental Health CenterShanghai Jiao Tong University School of MedicineShanghaiChina Shanghai I-Zhaohu Senior Care Services ShanghaiChina

Background The management of modifiable risk factors and comorbidities may impact the future trajectory of cognitive impairment,but easy-to-implement management methods are *** This study investigated the effects of simple but comprehensive cognitive health management practices on the cognitive function of older adults in the community with normal cognition(NC)and mild cognitive impairment(MCI).Methods The comprehensive cognitive health management programme included a psychiatric assessment of the cognitive risk factors for those in the intervention groups and individualised recommendations for reducing the risks through self-management supported by regular medical professional *** intervention groups for this study included 84 elderly participants with NC and 43 elderly participants with MCI who received comprehensive cognitive health *** control groups included 84 elderly participants with NC and 43 elderly participants with MCI who matched the intervention group’s general characteristics and scale scores using the propensity matching score *** Montreal Cognitive Assessment(MoCA)scale and Geriatric Depression Scale(GDS)scores were compared after a 1-year *** For older adults with MCI in the intervention group,MoCA scores were higher at the 1-year followup than at baseline(24.07(3.674)vs 22.21(3.052),p=0.002).For the MoCA subscales,the intervention group’s abstract and delayed memory scores had significantly increased during the 1-year ***,in a generalised linear mixed model analysis,the interaction effect of group×follow-up was statistically significant for the MCI group(F=6.61,p=0.011;coefficients=5.83).Conclusions After the comprehensive cognitive health management intervention,the older adults with MCI in the community showed improvement at the 1-year *** preliminary study was the first to demonstrate an easy-to-implement strategy for modifying the cognitive risk factors of elderl

关键词： impairment comprehensive management

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钒钛磁铁矿高炉冶炼的强化

钢铁 2000年第1期35卷 4-8,12页

作者：马家源孙希文盛世雄攀枝花钢铁(集团)公司

以高钛型钒钛磁铁矿为主要原料的攀钢高炉，在解决了泡沫渣、粘渣、铁水粘罐、铁损高、脱硫能力低等重要技术难题后，通过优化高炉操作及炉料结构，开发一系列强化冶炼的新技术，取得了大型高炉在采用难冶炼的特殊矿、入炉品位低的情况... 详细信息

以高钛型钒钛磁铁矿为主要原料的攀钢高炉，在解决了泡沫渣、粘渣、铁水粘罐、铁损高、脱硫能力低等重要技术难题后，通过优化高炉操作及炉料结构，开发一系列强化冶炼的新技术，取得了大型高炉在采用难冶炼的特殊矿、入炉品位低的情况下达到高利用系数的经验，获得了巨大的经济效益，并建立了高炉冶炼钒钛磁铁矿的系统理论。

关键词：钒钛磁铁矿高炉强化冶炼

BmEL-2 promotes TG metabolism by regulating BmAGPAT3 and BmFAF2 expression in Bombyx mori

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BmEL-2 promotes TG metabolism by regulating BmAGPAT3 and BmF...

中国蚕学会2023年学术年会

作者： Da Ma jiayuan Shi Si Zhou sheng Qin Muwang Li Xia Sun

Bombyx mori ELAV-like-1(BmEL-1) and Bombyx mori ELAV-like-2(BmEL-2) are two members of the ELAV-like family of RNA binding *** of Bmel-1 and Bmel-2 results in 5.8% and 28.5% decrease in larval weight on the third day of the fifth instar larva(L5D3) ***(TG) is the most important energy resource and also is the main component of neutral fat(NF) in *** investigate the role of Bmelav-like genes in synthesis and decomposition of TG,transcriptomic and metabolic analysis were performed on the first day of second instar larvae and L5D3 fat body of Bmel-1-and Bmel-2-mutants *** compared with control silkworm,differentially expressed genes generated in both mutants were mainly enriched in lysine degradation,fatty acid metabolism and biosynthesis of unsaturated fatty *** diglyceride(DG) and hosphatide(PA) contents were significantly decreased in Bmel-1-and Bmel-2-fat bodies than those of in ***,the NF content of both mutants fat body were reduced by 50% and 60% *** decrease of Bmel-2-NF content was attributed to reduced xpression of seven genes involved the TG *** of BmEL-2 promotes the expression of BmAGPAT3(*** 1-acyl-sn-glycero-3-phosphoacyltransferase gamma,LOC101741736) and BmFaF2(*** fatty acid synthetase-related factor 2,OC10173909) in Bm N cells,including mRNA and protein ***,the expression of Bmel-2,BmAGPAT3,and BmFaF2 were increased in BmN cells supplemented with sodium *** results suggest that BmEL-2 may be a key regulator of BmAGPAT3 and BmFAF2 expression,and then affect the synthesis of triglycerides in *** study provides a data basis for the study of lipid metabolism in silkworm,and also enriches the invertebrate lipid metabolism model.

关键词： RNA binding protein ELAV triglyceride fat body silkworm

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融合深度网络的改进快速生成超像素算法

计算机科学与探索 2020年第12期14卷 2132-2139页

作者：盛家川王佳媛李玉芝王君天津财经大学理工学院天津300222 天津财经大学管理科学与工程学院天津300222

超像素是图像过度分割的结果,提供了图像数据的中间级表示,对计算机视觉等领域的研究具有重要意义。现有的超像素算法是不可微的,且深度网络通常在规则的网格上进行定义,导致目前生成超像素的算法大多基于手工提取的像素特征进行。提出... 详细信息

超像素是图像过度分割的结果,提供了图像数据的中间级表示,对计算机视觉等领域的研究具有重要意义。现有的超像素算法是不可微的,且深度网络通常在规则的网格上进行定义,导致目前生成超像素的算法大多基于手工提取的像素特征进行。提出融合深度网络的改进快速生成超像素算法,将深度学习网络嵌入到超像素的生成过程中,首先利用含多隐含层的深度网络进行图像像素特征的提取,然后通过K-means聚类方法计算初始种子点位置以改善分割结果,在此基础上通过主动搜索方法确保像素标签的正确性,最后得到超像素分割结果。在Berkeley数据集BSDS500上,使用BSDS的基准测评与其他文献的对比实验表明,所提出的算法在分割结果的紧凑性、规则性等性能方面相对较好。

关键词：超像素分割深度网络聚类特征提取