On-chip manufacturing of synthetic proteins for point-of-care therapeutics
作者机构：Department of Chemical EngineeringVirginia TechBlacksburgVA 24061USA Department of Biological Systems EngineeringVirginia TechBlacksburgVA 24061USA
出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)
年 卷 期：2019年第5卷第1期
页 面：571-582页
核心收录：
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学]
主 题：potential. synthesis enable
摘 要：Therapeutic proteins have recently received increasing attention because of their clinical ***,most therapeutic proteins are produced on a large scale using various cell culture ***,storing and transporting these therapeutic proteins at low temperatures makes their distribution expensive and problematic,especially for applications in remote *** this end,an emerging solution is to use point-of-care technologies that enable immediate and accessible protein production at or near the patient’s *** we present the development of“Therapeutics-On-a-Chip(TOC),an integrated microfluidic platform that enables point-of-care synthesis and purification of therapeutic *** used fresh and lyophilized materials for cell-free synthesis of therapeutic proteins on microfluidic chips and applied immunoprecipitation for highly efficient,on-chip protein *** first demonstrated this approach by expressing and purifying a reporter protein,green fluorescent ***,we used TOC to produce cecropin B,an antimicrobial peptide that is widely used to control biofilmassociated *** successfully synthesized and purified cecropin B at 63 ng/μl within 6 h with a 92%purity,followed by confirming its antimicrobial functionality using a growth inhibition *** TOC technology provides a new platform for point-of-care production of therapeutic proteins at a clinically relevant quantity.
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