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Inhibition of botulinum toxin's association with rat brain synaptosomes by toosendanin,an antibotulismic triterpenoid

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Neural Regeneration Research 2006年第6期1卷 489-492页

作者： jianying zhou yuliang shi Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

BACKGROUND： Toosendanin （TSN）, a tnterpenoid derivative extracted from the bark of Melia toosendanin Sieb et Zucc, has been demonstrated to be an effective drug for treatment of experimental botulism. OBJECTIVE ： To explore its antibotulismic mechanism by observing the effect of toosendanin on association of botulinum toxin （BoTx） with rat brain synaptosomes under different conditions. DESIGN： A randomized controlled experiment SETTING： Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences MATERIALS： Sprague-Dawiey rats, weighing （220±12） g, were involved. TSN was a sample recrystallized in ethanol with a punty 〉 98%. BoTx/A and BoTx/C of 500 ku （1.8×10^7 mouse LD50/mg） were purchased from Wako （Japan）, Horse antitoxins to BoTx/A and/C （primary antibodies） were purchased from Lanzhou Institute of Biological Products （China）. METHODS ： Major experiments were finished between March 2005 and October 2005 in key laboratory of neurobiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. （1） Preparation of synaptosome of rats： one aliquot of synaptosome was pre-incubated with TSN （17 μmol/L） for 20 minutes at 4 % or 37 % （another aliquot of synaptosome which was untouched was used as control）. Then, BoTx was respectively added for another 20-minute incubation. （2） A homochronous expenment was still performed at 37 %. Differently, high level of K^＋ was used to stimulate synaptosome for 25 minutes. Two aliquots of synaptosomes with or without TSN （17 μmol/L）were preincubated for 15 minutes, Then, 30 mmol/L KCl was separately added in two aliquots, 5 minutes later, 13 nmol/L BoTx/C was separately added followed by 20-minute incubation. （3）The effect of TSN on BoTx binding was observed by Western blot and synchronization method. （4） t test was used for comparing the difference of measurement data. MAIN OUTCOME MEASURES ： The gray value of BoTx bands from western blot was used to estimate the bound amount of BoTx. RES

关键词： antibotulismic mechanism measurement data botulinum toxin Western blot type A

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Fault-sand combination modes and hydrocarbon accumulation in Binhai fault nose of Qikou Sag, Bohai Bay Basin, East China

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Petroleum Exploration and Development 2019年第5期46卷 919-934页

作者： zhou Lihong HAN Guomeng DONG Yueqi shi Qianru MA jianying HU Jinnan REN shichao zhou Kejia WANG Jincheng SI Weiliu Dagang Oilfield Company PetroChinaTianjin 300280China

Based on seismic and logging data,taking the downthrow fault nose of Binhai fault in Qikou Sag as the object of study,we analyzed fault characteristics,sand body distribution,fault-sand combinations and hydrocarbon accumulation to reveal the hydrocarbon enrichment law in the fault-rich area of fault depression lake *** results show that the Binhai Cenozoic fault nose is characterized by east-west zoning,the main part of the western fault segment is simple in structure,whereas the broom-shaped faults in the eastern segment are complex in structure,including several groups of *** difference of fault evolution controls the spatial distribution of sand *** sand bodies are in continuous large pieces in the downthrow fault trough belt along the Gangdong Fault in the middle segment of the fault nose,forming consequent fault-sand combination;whereas the fault activity period of the eastern part of the fault nose was later,and the sand bodies controlled by paleogeomorphology are distributed in multi-phase north-south finger-shaped pattern,forming vertical fault-sand combination pattern matching with the *** configuration between faults and sand bodies,and oil sources and caprocks determine the vertical conductivity,plane distribution and vertical distribution of oil and *** oil and gas accumulation modes,*** main fault hydrocarbon supply-fault sand consequent matching-oil accumulation in multi-layers stereoscopically and fault system transportation-fault sand vertical matching-oil accumulation in banded overlapping layers occur in the middle and eastern segments of the fault nose respectively,and they control the difference of oil and gas distribution and enrichment degree in the Binhai fault nose.

关键词： Bohai Bay Basin Qikou Sag Binhai fault nose sandstone development mechanism fault-sand combination oil and gas accumulation reservoir control mechanism

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Effects of the grinding process on the preparation and qualities of CWS

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Mining Science and Technology 2011年第6期21卷 767-771页

作者： yuliang Zhang Guoguang Wu Xianliang Meng Zengjie Yun Frank shi Yaqun He Xiaoqiang Luo School of Chemical Engineering and Technology China University of Mining ＆ Technology Xu.zhou 221008 China Julius Kruttschnitt Mineral Research Centre Brisbane 4068 Australia

Two different grinding processes were examined to determine the effect grinding has on the quality of a CWS. A series of slurries was prepared from Australian (Au) and Chinese (YZ) coals. Both types of coal were ground by a Chinese (CUMT) and an Australian (JK) grinding process. The performance tests of the prepared CWS showed that fluidity of all slurries was acceptable. The concentration of the CWS from YZ coal ground by the CUMT grinding process was higher than when the JK grinding process was used. The highest concentration was 70.14% in this case. The concentration of the CWS prepared from Au coal by the JK grinding process was higher than when the CUMT grinding process was used. The highest con- centration in this case was 70.97%. These differences are caused by the particle size distribution devel- oped during the different grinding processes.

关键词： Coal water slurry "Double-peak" gradation Slurry ability Grinding process Stability Fluidity

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Formation conditions and enrichment model of retained petroleum in lacustrine shale: A case study of the Paleogene in Huanghua depression, Bohai Bay Basin, China

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Petroleum Exploration and Development 2020年第5期47卷 916-930页

作者： ZHAO Xianzheng zhou Lihong PU Xiugang JIN Fengming shi Zhannan HAN Wenzhong JIANG Wenya HAN Guomeng ZHANG Wei WANG Hu MA jianying PetroChina Dagang Oilfield Company Tianjin 300280China

Compared with marine facies shale strata,lacustrine shale strata are more complicated in geological conditions,and thus more difficult to explore and *** realize economic exploration and development of lacustrine shale oil,the geological regularities of accumulation and high yield of retained movable petroleum in shale should be understood *** this work,taking the shale strata of Kong 2 Member and Sha 3 Member in the Paleogene of Huanghua depression in the Bohai Bay Basin as examples,based on the previous joint analysis results of over ten thousand core samples and the latest oil testing,production test and geochemical data of more than 30 horizontal wells,accumulation conditions and models of retained movable petroleum in lacustrine shale were studied *** study shows that at moderate organic matter abundance(with TOC from 2%to 4%),shale strata have the best match between oil content and brittleness,and thus are rich in oil and good in *** ancient lake basin size and moderate sediment supply intensity are the internal factors leading to best coupling of organic matter abundance and brittle mineral content in the shale *** thermal evolution maturity of Ro of 0.7%–1.0%(at burial depth of 3200 to 4300 m)is the interval where oil generation from thermal evolution and oil adsorption by kerogen in shale layers match best,and retained movable petroleum is high in *** diagenetic evolution stage(3200 to 4300 m in the middle diagenetic stage A)is conducive to the formation of a large number of dissolved pores and organic matter pores,which provide storage space for shale oil *** development degree of natural fractures(without damaging the shale oil roof and floor sealing conditions)is conducive to the storage,seepage and preservation of shale *** research results have overthrown the general understanding that high organic matter abundance,high maturity,and high development degree o

关键词： lacustrine shale retained hydrocarbon movable hydrocarbon shale oil enrichment model Kong 2 Member Sha 3 Member Huanghua Depression Bohai Bay Basin

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超临界氢气在弯管中的流动换热计算

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核动力工程 2019年第5期40卷 197-201页

作者：周彪吉宇孙俊孙玉良石磊清华大学核能与新能源技术研究院

为探究超临界氢气流经喷管喉部时的流动换热特性,通过ANSYS FLUENT软件模拟超临界氢气在180°弯管中的流动换热现象,得到了氢气在弯管中的流场分布以及不同位置处的壁面温度分布。研究发现:由于离心力作用,管内氢气流动在弯管段向外侧... 详细信息

为探究超临界氢气流经喷管喉部时的流动换热特性,通过ANSYS FLUENT软件模拟超临界氢气在180°弯管中的流动换热现象,得到了氢气在弯管中的流场分布以及不同位置处的壁面温度分布。研究发现:由于离心力作用,管内氢气流动在弯管段向外侧径向偏移,产生了垂直于主流方向的二次流现象,使得内侧氢气流速低于外侧;由于弯管段的流量分布不均,导致弯管外侧换热得到强化,内侧则出现传热恶化现象。在本文研究工况下,弯管段出口附近的内侧壁面区域,壁面温度达到最高,传热恶化最为显著。

关键词：核热推进超临界氢气弯管二次流传热恶化

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The Genome of Artemisia annua Provides Insight into the Evolution of Asteraceae Family and Artemisinin Biosynthesis

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Molecular Plant 2018年第6期11卷 776-788页

作者： Qian Shen Lida Zhang Zhihua Liao Shengyue Wang Tingxiang Yan Pu shi Meng Liu Xueqing Fu Qifang Pan yuliang Wang Zongyou Lv Xu Lu Fangyuan Zhang Weimin Jiang Yanan Ma Minghui Chen Xiaolong Hao Ling Li Yueli Tang Gang Lv Yan zhou Xiaofen Sun Peter E. Brodelius Jocelyn K.C. Rose Kexuan Tang Joint International Research Laboratory of Metabolic & Developmental Sciences Key Laboratory of Urban Agriculture (South) Ministry of Agriculture Plant Biotechnology Research Center Fudan-SJTU-Nottingham Plant Biotechnology R&D Center School of Agriculture and Biology Shanghai Jiao Tong University Shanghai 200240 China SWU-TAAHC Medicinal Plant Joint R&D Centre School of Life Sciences Southwest University Chongqing 400715 China Chinese National Human Genome Center at Shanghai Shanghai 201203 China Plant Biology Section School of Integrative Plant Science Cornell University Ithaca NY 14853 USA Department of Chemistry and Biomedical Sciences Linnaeus University 39182 Kalmar Sweden State Key Laboratory of Natural Medicines China Pharmaceutical University Nanjing 211198 China

Artemisia annua, commonly known as sweet wormwood or Qinghao, is a shrub native to China and has long been used for medicinal purposes. A. annua is now cultivated globally as the only natural source of a potent anti-malarial compound, artemisinin. Here, we report a high-quality draft assembly of the 1.74-gigabase genome of A. annua, which is highly heterozygous, rich in repetitive sequences, and contains 63 226 protein-coding genes, one of the largest numbers among the sequenced plant species. We found that, as one of a few sequenced genomes in the Asteraceae, the A. annua genome contains a large number of genes specific to this large angiosperm clade. Notably, the expansion and functional diversification of genes encoding enzymes involved in terpene biosynthesis are consistent with the evolution of the artemi- sinin biosynthetic pathway. We further revealed by transcriptome profiling that A. annua has evolved the sophisticated transcriptional regulatory networks underlying artemisinin biosynthesis. Based on compre- hensive genomic and transcriptomic analyses we generated transgenic A. annua lines producing high levels of artemisinin, which are now ready for large-scale production and thereby will help meet the chal- lenge of increasing global demand of artemisinin.

关键词： Artemisia annua artemisinin genome evolution transcriptome metabolic engineering

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Molecular detection and quantification of Xanthomonas albilineans in juice from symptomless sugarcane stalks using a real-time quantitative PCR assay

Molecular detection and quantification of Xanthomonas albili...

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中国植物病理学会2021年学术年会

作者： shi Yang ZHAO jianying zhou Jingru NTAMBO Mbuya Sylvain XU Pengyuan ROTT Philippe C. GAO Sanji National Engineering Research Center for Sugarcane Fujian Agriculture and Forestry University Département de phytotechnie Faculté des Sciences AgronomiquesUniversité de Lubumbashi CIRAD UMRPHIMF-34398 MontpellierFranceand PHIMPlant Health InstituteUniv MontpellierCIRADINRAEInstitut AgroIRD

Leaf scald,a bacterial disease caused by Xanthomonas albilineans(Ashby) Dowson,is a major limiting factor for sugarcane production *** identification and quantification of *** is a prerequisite for successful management of this disease.A very sensitive and robust qPCR assay was developed in this study for detection and quantification of *** using TaqMan probe and primers targeting a putative adenosine triphosphate-binding cassette(ABC) transporter gene(abc).The novel qPCR assay was highly specific to the 43 tested *** strains belonging to different pulsed-field gel electrophoresis(PFGE) *** detection thresholds were 100 copies/μL of plasmid DNA,100 fg/μL of bacterial genomic DNA,and 100 CFU/mL of bacterial suspension prepared from pure *** qPCR assay was 100 times more sensitive than a conventional PCR *** pathogen was detected by qPCR in 75.1%(410/546) symptomless stalk samples,whereas only28.4%(155/546) samples tested positive by conventional *** on qPCR data,population densities of *** in symptomless stalks of the same varieties differed between two sugarcane production areas in China,Beihai(Guangxi province) and Zhanjiang(Guangdong province).Nonsignificant correlation of *** populations was found between Beihai and *** newly developed qPCR assay proved to be highly sensitive and reliable for the detection and quantification of *** in sugarcane stalks.

关键词： Leaf scald molecular detection real-time quantitative PCR Saccharum spp. Xanthomonas albilineans

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多环芳烃类液体有机氢载体储放氢技术研究进展

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化工进展 2023年第2期42卷 1000-1007页

作者：周一鸣齐随涛周宇亮谭潇石利斌杨伯伦西安交通大学化学工程与技术学院陕西西安710049

基于“绿电+绿氢”的可再生能源规模化利用模式是实现碳中和目标的重要措施,然而目前现有的氢储运技术无法满足该利用模式的大规模与跨时空需求。多环芳烃类有机液体氢载体储氢被认为是最有可能实现大规模且安全高效的异地氢储运技术。... 详细信息

基于“绿电+绿氢”的可再生能源规模化利用模式是实现碳中和目标的重要措施,然而目前现有的氢储运技术无法满足该利用模式的大规模与跨时空需求。多环芳烃类有机液体氢载体储氢被认为是最有可能实现大规模且安全高效的异地氢储运技术。本文介绍了多环芳烃类不饱和有机液体储氢技术的基本原理与常用有机氢载体的物化性质,分析了作为有机氢载体的关键参数,在此基础上,从空间位阻效应的角度解释了多环芳烃类有机液体氢载体的环内与环间加脱氢反应机理,并进一步从活性组分分散度、表面电荷效应、氢溢流以及低配位数等方面对多环芳烃加脱氢催化剂的研究进展进行了总结。提出了目前多环芳烃类有机液体氢载体储氢的技术难点在于脱氢温度高、循环率低以及催化剂成本高,并对优化能量匹配、催化剂改性等未来的应用场景和发展方向进行了展望。

关键词：苯氢反应催化剂

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Sintilimab versus docetaxel as second-line treatment in advanced or metastatic squamous non-small-cell lung cancer:an open-label,randomized controlled phase 3 trial(ORIENT-3)

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Cancer Communications 2022年第12期42卷 1314-1330页

作者： Yuankai shi Lin Wu Xinmin Yu Puyuan Xing Yan Wang jianying zhou Airong Wang Jianhua shi Yi Hu Ziping Wang Guangyu An Yong Fang Sanyuan Sun Caicun zhou Changli Wang Feng Ye Xingya Li Junye Wang Mengzhao Wang Yunpeng Liu Yanqiu Zhao Ying Yuan Jifeng Feng Zhendong Chen Jindong shi Tao Sun Gang Wu Yongqian Shu Qisen Guo Yi Zhang Yong Song Shucai Zhang Yuan Chen Wei Li Hongrui Niu Wenwei Hu Lijun Wang Jianan Huang Yang Zhang Ying Cheng Zhengdong Wu Bo Peng Jiya Sun Christoph Mancao Yanqi Wang Luyao Sun Department of Medical Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing Key Laboratory of Clinical Study on Anticancer Molecular Targeted DrugsBeijingP.R.China Department II of Thoracic Medicine Hunan Cancer HospitalChangshaHunanP.R.China Department of Medical Oncology Zhejiang Cancer HospitalHangzhouZhejiangP.R.China Department of Respiratory Diseases The First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhouZhejiangP.R.China The Third Department of Chemotherapy Weihai Municipal HospitalWeihaiShandongP.R.China Department of Medical Oncology Linyi Cancer HospitalLinyiShandongP.R.China Oncology Department General Hospital of Chinese People’s Liberation ArmyBeijingP.R.China Department of Chest Medicine Beijing Cancer HospitalBeijingP.R.China Department of Oncology Beijing Chao-Yang HospitalCapital Medical UniversityBeijingP.R.China Department of Medical Oncology Sir Run Run Shaw HospitalZhejiang UniversityHangzhouZhejiangP.R.China Department of Medical Oncology Xuzhou Central HospitalXuzhou Medical UniversityXuzhouJiangsuP.R.China Department of Medical Oncology Shanghai Pulmonary HospitalTongji UniversityShanghaiP.R.China Department of Lung Cancer Tianjin Medical University Cancer Institute and HospitalTianjinP.R.China Department of Medical Oncology Cancer HospitalThe First Affiliated Hospital of Xiamen UniversitySchool of MedicineXiamen UniversityThe Third Clinical Medical CollegeFujian Medical UniversityXiamenFujianP.R.China Department of Medical Oncology The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenanP.R.China Department of Oncology Affiliated Hospital of Jining Medical UniversityJiningShandongP.R.China Department of Respiratory and Critical Care Medicine Peking Union Medical College HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijingP.R.China Department of Medical Oncology Key L

Background:Treatment options for Chinese patients with locally advanced or metastatic squamous-cell non-small-cell lung cancer(sqNSCLC)after failure of first-line chemotherapy are *** study(ORIENT-3)aimed to evaluate the efficacy and safety of sintilimab versus docetaxel as second-line treatment in patients with locally advanced or metastatic ***:ORIENT-3 was an open-label,multicenter,randomized controlled phase 3 trial that recruited patients with stage IIIB/IIIC/IV sqNSCLC after failure with first-line platinum-based *** were randomized in a 1:1 ratio to receive either 200 mg of sintilimab or 75 mg/m^(2) of docetaxel intravenously every 3 weeks,stratified by the Eastern Cooperative Oncology Group performance *** primary endpoint was overall survival(OS)in the full analysis set(FAS).Secondary endpoints included progression-free survival(PFS),objective response rate(ORR),disease control rate(DCR),duration of response(DoR)and ***:Between August 25,2017,and November 7,2018,290 patients were *** FAS,10 patients fromthe docetaxel armwere *** OS was 11.79(n=145;95%confidence interval[CI],10.28-15.57)months with sintilimab versus 8.25(n=135;95%CI,6.47-9.82)months with docetaxel(hazard ratio[HR]:0.74;95%CI,0.56-0.96;P=0.025).Sintilimab treatment significantly prolonged PFS(median 4.30 vs.2.79 months;HR:0.52;95%CI,0.39-0.68;P<0.001)and showed higher ORR(25.50%vs.2.20%,P<0.001)and DCR(65.50%vs.37.80%,P<0.001)than the docetaxel *** median DoRwas 12.45(95%CI,4.86-25.33)months in the sintilimab arm and 4.14(95%CI,1.41-7.23)months in the docetaxel arm(P=0.045).Treatment-related adverse events of grade≥3were reported in 26(18.1%)patients in the sintilimab arm and 47(36.2%)patients in the docetaxel *** biomarker analysis showed potential predictive values of expression levels of two transcription factors,including OVOL2(HR:0.35;P<0.001)and CTCF(HR:3.50;P<0.001),for sintilimab treatme

关键词： Non-small cell lung cancer Carcinoma squamous cell Sintilimab Immunotherapy Survival Randomized controlled trial

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“双一流”高校环境与资源类实验室化学品管理实践

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实验技术与管理 2020年第6期37卷 263-265,272页

作者：林森张建英史舟浙江大学国家级环境与资源实验教学示范中心浙江杭州310058 浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室浙江杭州310058

该文立足"双一流"背景下新工科和新农科对环境与资源类实验室建设要求,依据化学品分类、采购、使用和保管等化学品全过程安全监管原则,结合环境与资源类化学品管理的自身实践,提出并践行了责任制、信息化、集约式的闭环减量消危的化学... 详细信息

该文立足"双一流"背景下新工科和新农科对环境与资源类实验室建设要求,依据化学品分类、采购、使用和保管等化学品全过程安全监管原则,结合环境与资源类化学品管理的自身实践,提出并践行了责任制、信息化、集约式的闭环减量消危的化学品安全管理措施。通过三年的实践,在实验室化学品安全管理方面充分发挥了作用。

关键词： “双一流”建设实验室安全化学品管理

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