The Genome of Artemisia annua Provides Insight into the Evolution of Asteraceae Family and Artemisinin Biosynthesis
The Genome of Artemisia annua Provides Insight into the Evolution of Asteraceae Family and Artemisinin Biosynthesis作者机构：Joint International Research Laboratory of Metabolic & Developmental Sciences Key Laboratory of Urban Agriculture (South) Ministry of Agriculture Plant Biotechnology Research Center Fudan-SJTU-Nottingham Plant Biotechnology R&D Center School of Agriculture and Biology Shanghai Jiao Tong University Shanghai 200240 China SWU-TAAHC Medicinal Plant Joint R&D Centre School of Life Sciences Southwest University Chongqing 400715 China Chinese National Human Genome Center at Shanghai Shanghai 201203 China Plant Biology Section School of Integrative Plant Science Cornell University Ithaca NY 14853 USA Department of Chemistry and Biomedical Sciences Linnaeus University 39182 Kalmar Sweden State Key Laboratory of Natural Medicines China Pharmaceutical University Nanjing 211198 China
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2018年第11卷第6期
页 面：776-788页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种]
基 金：supported by the China National High Technology Research and Development Program China National Transgenic Plant Research and Commercialization Project Shanghai Jiao Tong University Agri-Engineering Program
主 题：Artemisia annua artemisinin genome evolution transcriptome metabolic engineering
摘 要：Artemisia annua, commonly known as sweet wormwood or Qinghao, is a shrub native to China and has long been used for medicinal purposes. A. annua is now cultivated globally as the only natural source of a potent anti-malarial compound, artemisinin. Here, we report a high-quality draft assembly of the 1.74-gigabase genome of A. annua, which is highly heterozygous, rich in repetitive sequences, and contains 63 226 protein-coding genes, one of the largest numbers among the sequenced plant species. We found that, as one of a few sequenced genomes in the Asteraceae, the A. annua genome contains a large number of genes specific to this large angiosperm clade. Notably, the expansion and functional diversification of genes encoding enzymes involved in terpene biosynthesis are consistent with the evolution of the artemi- sinin biosynthetic pathway. We further revealed by transcriptome profiling that A. annua has evolved the sophisticated transcriptional regulatory networks underlying artemisinin biosynthesis. Based on compre- hensive genomic and transcriptomic analyses we generated transgenic A. annua lines producing high levels of artemisinin, which are now ready for large-scale production and thereby will help meet the chal- lenge of increasing global demand of artemisinin.
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