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Overexpression of BnKCS1-1,BnKCS1-2,and BnCER1-2 promotes cuticular wax production and increases drought tolerance in Brassica napus

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The Crop Journal 2020年第1期8卷 26-37页

作者： Yanmei Wang Shurong Jin Yi Xu Shuai li Shuangjuan Zhang Zheng Yuan jiana li Yu Ni College of Agronomy and Biotechnology Academy of Agricultural SciencesSouthwest University

Higher amounts of cuticular wax in plants have been associated with improved plant stress tolerance and increased potential for industrial *** this study,orthologs of KCS1 and CER1 in Arabidopsis,designated BnKCS1-1,BnKCS1-2,and BnCER1-2,were isolated from Brassica *** of BnKCS1-1 and BnKCS1-2 in *** were induced by abscisic acid(ABA)and drought treatment,while transcription of BnCER1-2 was induced only by drought *** three gene transcripts decreased significantly when plants were treated with methyl jasmonate(MeJA)or subjected to cold *** of BnKCS1-1,BnKCS1-2,and BnCER1-2 under the control of the CaMV35S promoter led to a significant increase in cuticular wax on transgenic *** ***1-1 and BnKCS1-2 overexpression led to similar differences from non-transformed plants,with significantly higher levels of aldehydes(C29 and C30),alkanes(C28,C29,and C31)and secondary alcohols(C28 and C29),and a significantly lower level of C29 *** of BnCER1-2 led to an increase in alkanes(C27,C28,C29,and C31),a decrease in secondary alcohols(C28 and C29),and insignificant changes in other wax *** electron microscopy revealed that overexpression of BnKCS1-1,BnKCS1-2,and BnCER1-2 in *** resulted in a higher density of wax crystals on the leaf surface than observed in non-transformed *** plants showed a reduced rate of water loss and increased drought tolerance compared to non-transformed *** results suggest that BnKCS1-1,BnKCS1-2,and BnCER1-2 gene products can modify the cuticular wax of *** cuticular waxes using transgenic approaches is a new strategy for genetic improvement of plant drought tolerance and provides an opportunity for development of *** as a surface-wax crop.

关键词： Brassica napus Cuticular wax Drought tolerance Overexpression Transformation

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New Insights into Nested Long Terminal Repeat Retrotransposons in Brassica Species

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Molecular Plant 2013年第2期6卷 470-482页

作者： lijuan Wei Meili Xiaoa Zeshan An Bi Ma Annaliese S. Mason Wei Qian jiana li Donghui Fu Chongqing Engineering Research Center for Rapeseed College of Agronomy and Biotechnology Southwest University Chongqing 400716 China School of Agriculture and Food Sciences and centre for Integrative I~egume-Research The University of Queensland 1:he Universit'y of QueenslandBrisbane 4072 Australia Key Laboratory of Crop Physiology Ecology and Genetic Breeding Ministry of Education Agronomy College Jiangxi Agricultural University Nanchang330045 China

Long terminal repeat （LTR） retrotransposons, one of the foremost types of transposons, continually change or modify gene function and reorganize the genome through bursts of dramatic proliferation. Many LTR-TEs preferen-tially insert within other LTR-TEs, but the cause and evolutionary significance of these nested LTR-TEs are not well under-stood. In this study, a total of 1.52 Gb of Brassica sequence containing 2020 bacterial artificial chromosomes （BACs） was scanned, and six bacterial artificial chromosome （BAC） clones with extremely nested LTR-TEs （LTR-TEs density： 7.24/kb） were selected for further analysis. The majority of the LTR-TEs in four of the six BACs were found to be derived from the rapid proliferation of retrotransposons originating within the BAC regions, with only a few LTR-TEs originating from the proliferation and insertion of retrotransposons from outside the BAC regions approximately 5-23 Mya. LTR-TEs also pref-erably inserted into TA-rich repeat regions. Gene prediction by Genescan identified 207 genes in the 0.84Mb of total BAC sequences. Only a few genes （3/207） could be matched to the Brassica expressed sequence tag （EST） database, indicating that most genes were inactive after retrotransposon insertion. Five of the six BACs were putatively centromeric. Hence, nested LTR-TEs in centromere regions are rapidly duplicated, repeatedly inserted, and act to suppress activity of genes and to reshuffle the structure of the centromeric sequences. Our results suggest that LTR-TEs burst and proliferate on a local scale to create nested LTR-TE regions, and that these nested LTR-TEs play a role in the formation of centromeres.

关键词： LTR retrotransposons Brassica centromere retrotransposon-rich transposon burst.

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Broadening the genetic base of Brassica juncea by introducing genomic components from *** and *** via digenomic allohexaploid bridging

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The Crop Journal 2022年第3期10卷 672-679页

作者： Jiaqin Mei Jin liu Fang Yue Yangui Chen Jiayi Ming Zhiyong Xiong Fengqun Yu jiana li Wei Qian College of Agronomy and Biotechnology Southwest UniversityChongqing 400716China Academy of Agricultural Sciences Southwest UniversityChongqing 400716China State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University Chongqing 400715China Key Laboratory of Herbage and Endemic Crop Biotechnology School of Life SciencesInner Mongolia UniversityHohhot 010070Inner Mongoli1China Agriculture and Agri-Food Canad1 Saskatoon Research and Development Center107 Science PlaceSaskatoonSK S7N 0X2Canada

A narrow genetic base has hindered improvement of Brassica juncea(A^(j)A^(j)B^(j)B^(j)).In this study,large-scale genomic components were introduced from diploid ancestor species into modern *** using a digenomic hexaploid *** hexaploids A^(j)A^(j)A^(r)A^(r)B^(j)B^(j) and A^(j)A^(j)B^(j)B^(j)B^(n)B^(n) were first developed from ***×***(A^(r)A^(r))and ***×***(B^(n)B^(n)),and then crossed with dozens of *** and ***,*** types of hexaploid showed high pollen fertility and moderate seed set throughout the S_(1) to S_(3) generations,and could be crossed with diploid progenitor species under field conditions,in particular for the combination of A^(j)A^(j)B^(j)B^(j)B^(n)B^(n)×*** A^(j)A^(r)B^(n)B^(j)-type and 31 A^(j)A^(r)B^(n)B^(j)-type *** resources were generated,of which the A^(j)A^(r)B^(n)B^(j) type showed higher *** these new-type *** resources,97 individual plants were genotyped with 42 simple sequence repeat markers,together with 16 current *** accessions and 30 hexaploid *** on 180 polymorphic loci,the new-type *** resources and current *** were separated clearly into distinct groups,with large genetic distance between the new-type *** resources and current *** study provides a novel approach to introducing large-scale genomic components from diploid ancestor species into ***.

关键词： Brassica juncea Diploid progenitor species Exotic introgression Genetic diversity Digenomic hexaploid

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Identification and characterization of a curly-leaf locus CL1 encoding an IAA2 protein in Brassica napus

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The Crop Journal 2023年第3期11卷 756-765页

作者： Yingchao Tan Lanyang Ren Jia Wang Shuyao Ran liusha Wu Ziyi Cheng Cunmin Qu jiana li liezhao liu College of Agronomy and Biotechnology Southwest UniversityChongqing 400715China Chongqing Engineering Research Center for Rapeseed Academy of Agricultural SciencesState Cultivation Base of Crop Stress Biology for Southern Mountainous LandSouthwest UniversityChongqing 400715China

The leaf is the main organ for rapeseed photosynthesis,and its morphology influences photosynthetic efficiency and supports increased planting density and ***,the molecular regulatory mechanism of leaf morphology in Brassica napus is poorly understood,restricting progress in breeding for the *** describe a novel dominant mutation,curly leaf 1(cl1),which confers uneven dorsal–ventral axis development,irregular cellular structure and influenced gravitropic response in the seedling *** CL1 locus was mapped to a 1.573-Mb interval on chromosome A05 using simple sequence repeat(SSR)markers,and co-segregated with the phenotype of plants in the curly F2 population.A substitution(P62S)was identified in the highly conserved degron motif(GWSPV)of the IAA2 protein in the cl1mutant,and the P62S substitution impaired the interaction between IAA2 and TIR1 in the presence of auxin,influencing auxin *** P62S substitution-induced curly leaf phenotype was verified by ectopic expression of Bna ***2 in Arabidopsis and *** findings explain the function of IAA2 in rapeseed,providing a foundation for future investigation of auxin signaling and the mechanisms underlying leaf development in ***.

关键词： Brassica napus Curly leaf Auxin IAA2 protein

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Development of a target capture sequencing SNP genotyping platform for genetic analysis and genomic breeding in rapeseed

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The Crop Journal 2023年第2期11卷 499-510页

作者： Xiaodong li Xumei liu Yonghai Fan Shengting li Mengna Yu Mingchao Qian Yuling Chen Hongqiao Chen Xinchun li Bei liu Xinfu Xu Cunmin Qu jiana li Kun Lu Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science City and Southwest University College of Agronomy and BiotechnologySouthwest UniversityBeibeiChongqing 400715China Engineering Research Center of South Upland Agriculture Ministry of EducationChongqing 400715China Academy of Agricultural Sciences Southwest UniversityBeibeiChongqing 400715China China Golden Marker(Beijing)Biotech Co. Ltd.Beijing 102206China

Rapeseed(Brassica napus)is an oil crop grown worldwide,making it a key plant species in molecular breeding ***,the complexity of its polyploid genome increases sequencing costs and reduces sequencing *** capture coupled with high-throughput sequencing is an efficient approach for detecting genetic variation at genomic regions or loci of *** this study,588 resequenced accessions of rapeseed were used to develop a target capture sequencing SNP genotyping platform named *** platform comprised 54,765,with 54,058 resequenced markers from the pan-genome,and 855 variant trait-associated markers for 12 agronomic *** capture quality of BnaPan50T was demonstrated well in 12 typical *** with a conventional genotyping array,BnaPan50T has a high SNP density and a high proportion of SNPs in unique physical positions and in annotated functional genes,promising wide *** capture sequencing and wholegenome resequencing in 90 doubled-haploid lines yielded 60%specificity,78%uniformity within tenfold coverage range,and 93%genotyping accuracy for the ***50T was used to construct a genetic map for quantitative trait loci(QTL)mapping,identify 21 unique QTL,and predict several candidate genes for yield-related traits in multiple environments.A set of 132 core SNP loci was selected from BnaPan50T to construct DNA fingerprints and germplasm identification *** study provides genomics resources to support target capture sequencing,genetic analysis and genomic breeding of rapeseed.

关键词： Rapeseed Target capture SNP genotyping platform Genomic breeding

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Genome-wide identi?cation of loci affecting seed glucosinolate contents in Brassica napus L.

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Journal of Integrative Plant Biology 2019年第5期61卷 611-623页

作者： Dayong Wei Yixin Cui Jiaqin Mei Lunwen Qian Kun Lu Zhi-Min Wang jiana li Qinglin Tang Wei Qian College of Horticulture and Landscape Architecture Southwest UniversityChongqing 400715China Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of EducationChongqing 400715China College of Agronomy and Biotechnology Southwest UniversityChongqing 400715China Academy of Agricultural Sciences Southwest UniversityChongqing 400715China Collaborative Innovation Center of Grain and Oil Crops in South China Hunan Agricultural UniversityChangsha 410128China

Glucosinolates are amino acid-derived secondary metabolites that act as chemical defense agents against ***,the presence of high levels of glucosinolates severely diminishes the nutritional value of seed meals made from rapeseed(Brassica napus L.).To identify the loci affecting seed glucosinolate cont ent(SGC),we con ducted genome-wide resequencing in a population of 307 diverse *** accessions from the three *** ecotype groups,namely,spring,winter,and *** resequencing data were used for a genome-wide association study(GWAS)to identify the loci affecting *** the three ecotype groups,four comm on and four ecotype-specific haplotype blocks(HBs)were significantly associated with *** identify candidate genes controlling SGC,transcriptome analysis was carried out in 36 accessions showing extreme SGC *** of haplotypes,genomic variation,and candidate gene expression pointed to five and three candidate genes in the common and spring group-specific HBs,*** expression analyses dem on strated that additive effects of the three candidate genes in the spring group-specific HB play important roles in the SGC of ***.

关键词： Glucosinolates are amino defense agents against group-specific HB

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Genome-Wide Identification and Expression Profiling Suggest that Invertase Genes Function in Silique Development and the Response to Sclerotinia sclerotiorum in Brassica napus

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Phyton-International Journal of Experimental Botany 2020年第2期89卷 253-273页

作者： Jingsen liu Jinqi Ma Ai lin Chao Zhang Bo Yang liyuan Zhang lin Huang jiana li Chongqing Rapeseed Engineering Research Center College of Agronomy and BiotechnologyChongqing400715China Academy of Agricultural Sciences Southwest UniversityChongqing400715China

Invertase(INV),a key enzyme in sucrose metabolism,irreversibly catalyzes the hydrolysis of sucrose to glucose and fructose,thus playing important roles in plant growth,development,and biotic and abiotic stress *** this study,we identified 27 members of the BnaINV family in Brassica *** constructed a phylogenetic tree of the family and predicted the gene structures,conserved motifs,cis-acting elements in promoters,physicochemical properties of encoded proteins,and chromosomal distribution of the *** also analyzed the expression of the BnaINVs in different tissues and developmental stages in the *** cultivar Zhongshuang 11 using *** addition,we analyzed RNA-sequencing data to explore the expression patterns of the BnaINVs in four cultivars with different harvest indices and in plants inoculated with the pathogenic fungus Sclerotinia *** used WGCNA(weighted coexpression network analysis)to uncover BnaINV regulatory ***,we explored the expression patterns of several BnaINV genes in cultivars with long(Zhongshuang 4)and short(Ningyou 12)*** results suggest that BnaINVs play important roles in the growth and development of rapeseed siliques and the defense response against *** findings could facilitate the breeding of high-yielding *** cultivars with strong disease resistance.

关键词： Brassica napus invertase Sclerotinia WGCNA RNA-seq

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Two types of cinnamoyl-Co A reductase function divergently in accumulation of lignins,flavonoids and glucosinolates and enhance lodging resistance in Brassica napus

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The Crop Journal 2022年第3期10卷 647-660页

作者： Nengwen Yin Bo li Xue liu Ying liang Jianping lian Yufei Xue Cunmin Qu Kun Lu lijuan Wei Rui Wang jiana li Yourong Chai Chongqing Engineering Research Center for Rapeseed Chongqing Key Laboratory of Crop Quality ImprovementCollege of Agronomy and BiotechnologySouthwest UniversityChongqing 400715China Engineering Research Center of South Upland Agriculture of Ministry of Education Academy of Agricultural SciencesSouthwest UniversityChongqing 400715China

Brassica crops,which are of worldwide importance,provide various oil,vegetable and ornamental products,as well as feedstocks for animal husbandry and biofuel ***-Co A reductase(CCR)is the entry point to the lignin pathway and a crucial locus in manipulation of associated traits,but CCRassociated metabolism and traits in Brassica crops have remained largely unstudied except in Arabidopsis *** report the identification of 16 CCR genes from Brassica napus and its parental species *** and *** Bn CCR1 and Bn CCR2 subfamilies displayed divergent organ-specificity and participation in the yellow-seed *** functions were dissected via overexpression of representative paralogs in *** CCR1 was expressed preferentially in G-and H-lignin biosynthesis and vascular development,while Bn CCR2 was expressed in S-lignin biosynthesis and interfascicular fiber *** CCR1 showed stronger effects on lignification-related development,lodging resistance,phenylpropanoid flux control,and seed coat pigmentation,whereas Bn CCR2 showed a stronger effect on sinapate *** CCR1 upregulation delayed bolting and flowering time,while Bn CCR2 upregulation weakened the leaf vascular system in consequence of suppressed G-lignin *** CCR1 and Bn CCR2 were closely but almost oppositely linked with glucosinolate metabolism via inter-pathway *** conclude that Bn CCR1 and Bn CCR2 subfamilies offer great but differing potential for manipulating traits associated with phenylpropanoids and *** study reveals the CCR1–CCR2 divergence in Brassicaceae and offers a resource for rapeseed breeding for lodging resistance,yellowseed traits,and glucosinolate traits.

关键词： Brassica napus Cinnamoyl-CoA reductase(CCR) lignin Flavonoids Lodging

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MICRODISSECTION OF MINIATURE PIG EAR

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Journal of Otology 2013年第2期8卷 91-96页

作者： YI Haijin GUO Weiwei CHEN Lei WU Na li jiana REN lili YANG Shiming Department of Otolaryngology Head and Neck SurgeryInstitute of Otolaryngology Chinese PLA General Hospital Department of Neurosurgery Division Otolaryngology- Head and Neck Surgery Beijing Tiantan Hospital Capital University of Medical Science State Key Laboratory for Agro- Biotechnology China Agricultural University

Objective To investigate the suitability of miniature pigs as an animal model for otological research. Methods Microdissection of the temporal bone was performed on 10 miniature pigs and recorded on photo-graphs. Results The morphology and measurement of the external, middle and inner ear and the lateral re-cess of the miniature pigs were obtained by microdissection. Conclusion Compared to traditional animal models, the miniature pig may be a better model for biomedical research because of its many similarities in physiological functions with humans. Similarities of the temporal bone structures, including the external, middle and inner ear and the lateral recess, between the miniature pig and human make the animal a poten-tially useful model for otological research.

关键词： Miniature pig animal model otological research.

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Genome-wide association study identifies novel loci and candidate genes for drought stress tolerance in rapeseed

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Oil Crop Science 2021年第1期6卷 12-22页

作者： Ali Shahzad Minchao Qian Bangyang Sun Umer Mahmood Shengting li Yonghai Fan Wei Chang lishi Dai Hong Zhu jiana li Cunmin Qu Kun Lu College of Agronomy and Biotechnology Southwest UniversityChongqing400715China Academy of Agricultural Sciences Southwest UniversityChongqing400715China Engineering Research Center of South Upland Agriculture Ministry of EducationChongqing400715China College of life Sciences Yangtze UniversityJingzhou434025HubeiChina

Rapeseed(Brassica napus)is one of the most important oil crops worldwide;however,drought seriously curtails its growth and *** drought-tolerant germplasm is an efficient strategy for addressing water ***,we phenotyped a panel of 264 *** accessions at full-bloom stage using water loss ratio(WLR)as drought-tolerant *** identified 8 low-WLR and 6 high-WLR accessions,regarded as droughttolerant and drought-sensitive,*** with drought-sensitive accessions at the seedling stage,drought-tolerant accessions had shown better performance in maintaining fresh and dry weights,and performed the higher expression of drought-induced marker genes under drought ***,a total of 139 SNPs(single nucleotide polymorphisms)were identified associated with the WLR using a genome-wide association study(GWAS)among 264 *** accessions,with the largest number SNPs at chromosome A10,and 13 SNPs significantly were associated with the WLR(-log_(10)(p-value)>6).Furthermore,4 putative candidate genes(***6,***,***5 and ***)were screened involving in drought tolerance in ***,our results highlight the WLR's importance in drought tolerance and establish the foundation for improving WLR-associated drought tolerance in rapeseed.

关键词： GWAS Water loss ratio Drought SNP Rapeseed

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