Genome-Wide Identification and Expression Profiling Suggest that Invertase Genes Function in Silique Development and the Response to Sclerotinia sclerotiorum in Brassica napus

作     者：Jingsen Liu Jinqi Ma Ai Lin Chao Zhang Bo Yang Liyuan Zhang Lin Huang Jiana Li 

作者机构：Chongqing Rapeseed Engineering Research CenterCollege of Agronomy and BiotechnologyChongqing400715China Academy of Agricultural SciencesSouthwest UniversityChongqing400715China 

出 版 物：《Phyton-International Journal of Experimental Botany》 (国际实验植物学杂志（英文）)

年 卷 期：2020年第89卷第2期

页      面：253-273页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0901[农学-作物学] 0703[理学-化学] 100214[医学-肿瘤学] 0902[农学-园艺学] 10[医学] 

基　　金：This work was supported by grants from the National Natural Science Foundation of China(31830067) National Key Research and Development Program of China(2018YFD0100504-05) the“111”Project(B12006) 

主　　题：Brassica napus invertase Sclerotinia WGCNA RNA-seq 

摘      要：Invertase(INV),a key enzyme in sucrose metabolism,irreversibly catalyzes the hydrolysis of sucrose to glucose and fructose,thus playing important roles in plant growth,development,and biotic and abiotic stress *** this study,we identified 27 members of the BnaINV family in Brassica *** constructed a phylogenetic tree of the family and predicted the gene structures,conserved motifs,cis-acting elements in promoters,physicochemical properties of encoded proteins,and chromosomal distribution of the *** also analyzed the expression of the BnaINVs in different tissues and developmental stages in the *** cultivar Zhongshuang 11 using *** addition,we analyzed RNA-sequencing data to explore the expression patterns of the BnaINVs in four cultivars with different harvest indices and in plants inoculated with the pathogenic fungus Sclerotinia *** used WGCNA(weighted coexpression network analysis)to uncover BnaINV regulatory ***,we explored the expression patterns of several BnaINV genes in cultivars with long(Zhongshuang 4)and short(Ningyou 12)*** results suggest that BnaINVs play important roles in the growth and development of rapeseed siliques and the defense response against *** findings could facilitate the breeding of high-yielding *** cultivars with strong disease resistance.