Identification and characterization of a curly-leaf locus CL1 encoding an IAA2 protein in Brassica napus

作     者：Yingchao Tan Lanyang Ren Jia Wang Shuyao Ran Liusha Wu Ziyi Cheng Cunmin Qu Jiana Li Liezhao Liu Yingchao Tan;Lanyang Ren;Jia Wang;Shuyao Ran;Liusha Wu;Ziyi Cheng;Cunmin Qu;Jiana Li;Liezhao Liu

作者机构：College of Agronomy and BiotechnologySouthwest UniversityChongqing 400715China Chongqing Engineering Research Center for RapeseedAcademy of Agricultural SciencesState Cultivation Base of Crop Stress Biology for Southern Mountainous LandSouthwest UniversityChongqing 400715China 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2023年第11卷第3期

页      面：756-765页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China(31971902,32001509) China Agriculture Research System of MOF and MARA 

主　　题：Brassica napus Curly leaf Auxin IAA2 protein 

摘      要：The leaf is the main organ for rapeseed photosynthesis,and its morphology influences photosynthetic efficiency and supports increased planting density and ***,the molecular regulatory mechanism of leaf morphology in Brassica napus is poorly understood,restricting progress in breeding for the *** describe a novel dominant mutation,curly leaf 1(cl1),which confers uneven dorsal–ventral axis development,irregular cellular structure and influenced gravitropic response in the seedling *** CL1 locus was mapped to a 1.573-Mb interval on chromosome A05 using simple sequence repeat(SSR)markers,and co-segregated with the phenotype of plants in the curly F2 population.A substitution(P62S)was identified in the highly conserved degron motif(GWSPV)of the IAA2 protein in the cl1mutant,and the P62S substitution impaired the interaction between IAA2 and TIR1 in the presence of auxin,influencing auxin *** P62S substitution-induced curly leaf phenotype was verified by ectopic expression of Bna ***2 in Arabidopsis and *** findings explain the function of IAA2 in rapeseed,providing a foundation for future investigation of auxin signaling and the mechanisms underlying leaf development in ***.