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Investigation of the structural and dynamic basis of kinesin dissociation from microtubule by atomistic molecular dynamics simulations

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Chinese Physics B 2022年第5期31卷 756-765页

作者： jian-gang wang Xiao-Xuan Shi Yu-Ru Liu Peng-Ye wang Hong Chen Ping Xie School of Material Science and Engineering Central South University of Forestry and TechnologyChangsha 410004China Key Laboratory of Soft Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China

Kinesin is a molecular motor that can step processively on microtubules via the hydrolysis of ATP *** important factor characterizing the processivity of the kinesin motor is its dissociation from the ***,using all-atom molecular dynamics simulations,we studied the dissociation process of the kinesin head in weak-microtubulebinding or ADP state from tubulin on the basis of the available high-resolution structural data for the head and *** analyzing the simulated snapshots of the structure of the head-tubulin complex we provided detailed structural and dynamic information for the dissociation *** found that the dissociation of the head along different directions relative to the tubulin exhibits very different dynamic ***,the potential forms or energy landscapes of the interaction between the head and tubulin along different directions were *** studies have important implications for the detailed molecular mechanism of the dissociation of the kinesin motor and thus are critical to the mechanism of its processivity.

关键词： kinesin dissociation energy landscape molecular dynamics simulation

Gecko crude peptides inhibit migration and lymphangiogenesis by down regulating the expression of VEGF-C in human hepatocellular carcinoma cells and human lymphatic endothelial cells

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中国药理学与毒理学杂志 2017年第10期31卷 958-959页

作者： Meng-li GUO Cai-e wang Yi-meng DUAN jian-gang wang Medical College Henan University of Science and Technology The First Affiliated Hospital and College of Clinical Medicine of Henan University of Science and Technology

OBJECTIVE To explore the role of gecko crude peptides(GCPs)in the proliferation,apoptosis,migration and lymphangiogenesis of human hepatocellular carcinoma cells(Hep G2)and human lymphaticendothelial cells(HLECs)in *** The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay was used to evaluate the anti-proliferative effect of GCPs and si RNA-VEGF-C on Hep G2 cells,Hoechst 33258 staining and flow cytometry were performed to analyze cycle and *** migration and invasion ability of cells were assayed by transwell chamber experiment and wound-healing *** protein and m RNA expressions of vascular endothelial growth factor-C(VEGF-C)and CXC chemokine receptor-4(CXCR4)were detected by q-PCR,immunofluorescence,Western *** protein expressions of the extracellular signal regulated kinase(ERKI/2),c-Jun N-terminal kinase(JNK),p38-mitogen activated protein kinases(p38 MAPK),serine/threonine kinase(Akt)and phosphatidylinositol-3-kinase(PI3K)were detected by western *** anti-lymphangiogenesis effect of GCPs on the HLECs was analyzed using an in vitro tube-formation *** protein and m RNA expressions of vascular endothelial growth factor receptor-3(VEGFR-3)and stromal cell-derived factor-1(SDF-1)were detected by q-PCR,Western *** GCPs and si RNA-VEGF-C inhibited Hep G2 proliferation,invasion and migration,and the most obvious inhibitory effect was both synergistic ***,GCPs suppressed HLECs proliferation,migration and tubelike structure formationin a dose-dependent manner,and had inhibitory effect of tumor-induced lymphangiogenesis in ***,we found that GCPs and si RNA-VEGF-C decreased the expressions of MMP-2,MMP-9,VEGF-C,CXCR4,phospho-ERK1/2,phospho-P38,phospho-JNK and PI3K in Hep G2 ***,GCPs had a dose-dependent depressive effecton the expressions of VEGFR-3,SDF-1 in *** The low expression of VEGF-C mediated by si RNA-VEGF-C and GCPs inhibit tumor proliferation,invas

关键词： gecko crude peptides hepatic carcinoma vascular endothelial growth factor-C RNA interference(RNAi) lymphangiogenesis

The extended RBAC model based on grid computing

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The Journal of China Universities of Posts and Telecommunications 2006年第3期13卷 93-97页

作者： CHEN jian-gang wang Ru-chuan wang Hai-yan Department of Computer Science and Technology Nanjing University of Postsand Telecommunications Nanjing 210003 China

This article proposes the extended role-based access control （RBAC） model /br solving dynamic and multidomain problems in grid computing, The formulated description of the model has been provided. The introduction of context and the mapping relations of context-to-role and context-to-permission help the model adapt to dynamic property in grid environment. The multidomain role inheritance relation by the authorization agent service realizes the multidomain authorization amongst the autonomy domain. A function has been proposed for solving the role inheritance conflict during the establishment of the multidomain role inheritance relation.

关键词： grid computing RBAC context multi-domain

Long-term outcome of patients with atrial myxoma after surgical intervention: analysis of 403 cases

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Journal of Geriatric Cardiology 2019年第4期16卷 338-343页

作者： Chen-Xi jianG jian-gang wang Rui-Dong QI Wei wang Li-jian GAO Jing-Hua ZHAO Chun-Xiao ZHANG Meng-Chen ZHOU Xin TU Mei-Sheng SHANG Yan YAO Department of Cardiology Beijing Anzhen HospitalCapital Medical UniversityBeijingChina Department of Cardiovascular Surgery Beijing Anzhen HospitalCapital Medical UniversityBeijingChina Center for Coronary Heart Disease Department of CardiologyCardiovascular Institute & Fuwai HospitalCAMS & PUMCNational Center for Heart DiseaseBeijingChina College of Life Science and Technology Huazhong University of Science and TechnologyWuhanChina

Objective To assess long-term survival and late cardiovascular events in patients with atrial myxoma after surgical intervention. Methods Retrospective analysis of 403 patients undergoing resection of atrial myxoma from January 2002 to December 2016 was conducted with a median follow-up period of 4.5 (range: 0.5?15) years. Results The cross-clamp time and cardiopulmonary bypass times were 41.1 ± 21.4 and 65.2 ± 27.3 min,respectively. A diagnosis of myxoma was histopathologically confirmed in all cases. The early in-hospital mortality rate was 0.7%(n = 3). During the follow-up period,tumor recurrence occurred in six patients and cerebral infarction in nine. There were 48 (11.9%) patients with late onset atrial fibrillation (AF). By multivariate analysis,age (HR = 1.05,95% CI: 1.02–1.09,P < 0.001),left atrial diameter (HR = 1.23,95% CI: 1.08–1.36,P = 0.012),and mitral valve surgery (HR = 1.17,95% CI: 1.05–1.29,P = 0.027) were independent predictors of late onset AF. Twenty-one (5.2%) patients died during the follow-up period. Advanced age (HR = 1.07,95% CI: 1.04–1.10,P = 0.003) and multiple surgical procedures (HR = 1.18,95% CI: 1.06–1.29,P = 0.012) were significantly associated with overall mortality. Conclusions Atrial myxoma can be resected with good long-term survival. Late onset AF is common after surgery in patients with atrial myxoma. Advanced age,left atrial diameter,and mitral valve surgery were independent predictors of outcomes.

关键词： Atrial fibrillation Atrial myxoma Embolism Survival

Saikosaponins-b suppresses tumor growth and angiogenesis of hepatocellular carcinoma by regulating VEGF/ERK/HIF-1α signal pathway

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中国药理学与毒理学杂志 2017年第10期31卷 962-963页

作者： Rui-fang LI Jun-min FU Xing-zhi LYU Zi-han GAO Hong-wei wang jian-gang wang Laboratory of Pharmacology and Molecular Biology Medical CollegeHenan University of Science and Technology School of Nursing Henan University of Science and Technology

OBJECTIVE Angiogenesis therapy has attracted interest as a potential treatment for hepatocellular carcinoma(HCC).In this study,we investigated the anti-proliferative activities and antiangiogenesis effects of saikosaponins(SS)-b on hepatocellular carcinoma(HCC)and its regulation on VEGF/ERK/HIF-1 αsignal *** H22 hepatoma-bearing mice model and HepG-2 cells were used to study the anti-tumor and anti-angiogenesis effects of SS-b in vivo and in *** change of tumor tissue was observed by HE staining,the microvascular changes were detected by immunohistochemical *** effects of SS-b on angiogenesis were examined by using the chick embryo chorioallantoic membrane(CAM)*** effects of SS-b on proliferation,migration and invasion were investigated by MTT assay,scratch wound healing assay and transwell assay inhuman umbilical vein endothelial cell(HUVEC)and HepG2 cells in *** endothelial growth factor(VEGF),matrix metalloproteinase-2/9(MMP-2/9),hypoxia-inducible factor-1α(HIF-1α)expression and the phosphorylation of extracellular regulated kinase(ERK)were analyzed using RT-PCR and *** SS-b effectively inhibited the tumor growth of H22 mice in *** inhibitory rate of tumor was 49.1%,50.7%,66.1%in SS-b 5,10 and 20 mg·kg-1group *** staining results showed that SS-b induced tumor necrosis and nuclear dissolution in H22 ***,SS-b also reduced the number of microvessels of tumor tissue in H22 mice significantly and suppressed the angiogenesis of CAM induced by ***-b had an obvious inhibitory effect on cell proliferation,migration and invasion of HUVEC cells and HepG-2 *** effects were associated with downregulation of the expression of MMP2/9 and suppression of VEGF/ERK/HIF-1αsignaling in H22 mice and Hep-G2 *** Our findings showed that SS-b exerts anti-tumor effects by inhibiting tumor angiogenesis via regulating VEGF/ERK/HIF-1α signal pathway in vivo and in vitro.

关键词： saikosaponins-b angiogenesis hepatocellular carcinoma chorioallantoic membrane HUVEC cells

Saikosaponin-b regulates the proliferation and apoptosis of HepG2 cells by targeting the MACC1/c-Met/Akt signaling pathway

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中国药理学与毒理学杂志 2017年第10期31卷 965-966页

作者： Xing-zhi LYU Rui-fang LI Zi-han GAO Hong-wei wang Sang-qiang LI jian-gang wang Laboratory of Pharmacology and Molecular Biology Medical CollegeHenan University of Science and Technology School of Nursing Henan University of Science and Technology

OBJECTIVE Metastasis-associated in colon cancer-1(MACC1)is an oncogene that has been newly *** promotes tumor proliferation and invasion via the MET *** study investigated the effects of Saikosaponin-b(SS-b)on the proliferation and apoptosis of HepG2 cells and its regulation on MACC1/c-Met/Akt signaling *** HepG2 cells were treated with SS-b(10-800 g·L^(-1))for 48 h in *** CCK-8 assay was used to assess cell proliferation,and cell apoptosis was determined by Hoechst33258 staining,AnnexinⅤ/PI staining and caspase 3 ***-PCR was used to examine the expression of MACC1,c-MET and hepatocyte growth factor(HGF)mR ***1 protein was detected by Western blot and *** protein expressions of p-cMET,c-MET,p-AKT,AKT,p-BAD,BAD were measured by Western *** SS-b inhibited the growth of HepG2 cells in dose-dependent way and induced cell apoptosis ***2 cells showed karyopyknosis,fragmentation and fluorescence highlight in SS-b treatment *** results showed that apoptosis rate of HepG2 cells increased with SS-b *** immunofluorescence results showed that the MACC1 expression decreased significantly in HepG2 cells treated with *** expression levels of MACC1,c-MET and HGF mR NA in HepG2 cells were significantly inhibited by ***-b also significantly decreased the protein expressions of MACC1,p-c-MET and p-AKT while increased the expression of p-BAD and caspase 3 in HepG2 cells(P<0.05).CONCLUSION SS-b inhibited the proliferation and induced the apoptosis of HepG2 cells by targeting the MACC1/c-Met/Akt signaling pathway.

关键词： saikosaponin-b metastasis-associated in colon cancer-1 c-Met signaling hepatocel ular carcinoma

Imagination and Emotion in William Blake＇s Poems

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Journal of Literature and Art Studies 2016年第1期6卷 17-22页

作者： XU jian-gang HUANG Rong wang Dong-qing China Three Gorges University Yichang China

Writer＇s imagination does not only provide inspiration for literary creation, but also contribute literature works with more dazzling brilliance. As a representative of the British Romantic Poetry--William Blake, whose poems are full of imagination, always concedes his emotion and thoughts in a variety of imageries, exposing the reality of his times within his poetry writing. However. William Blake＇s imagination is no castles in the air, but based on the religious mythology, historical background, and the poet＇s life experience and dream pursuit. Only with in-depth understanding of the imagination in William Blake＇s poems can those hidden emotions and thoughts be grasped and appreciated.

关键词： imagination emotion William Blake

Antitumor effect and mechanism of Gecko on human esophageal carcinoma cell lines in vitro and xenografted sarcoma 180 in Kunming mice

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World Journal of Gastroenterology 2008年第25期14卷 3990-3996页

作者： Fei Liu jian-gang wang Shu-Ying wang Yan Li Yin-Ping Wu Shou-Min Xi Medical College Henan Universityof Science & Technology Luoyang 471003 Henan ProvinceChina Medical Department the First Affiliated Hospital ofHenan Science and Technology University Luoyang 471001Henan Province China

AIM: To investigate the anti-tumor effect of Chinese medicine Gecko on human esophageal carcinoma cell lines and xenografted sarcoma 180 in Kunming mice and its mechanism. METHODS: The serum pharmacological method was used in vitro . The growth rates of the human esophageal carcinoma cells (EC9706 or EC1) were measured by a modifi ed 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The transplanted tumor model of the mouse S180 sarcoma was established. Fifty mice were randomly divided into fi ve groups (n = 10). Three Gecko groups were treated respectively with oral administration of Gecko powder at a daily dose of 13.5 g/kg, 9 g/kg, and 4.5 g/kg. The negative group (NS group) was treated with oral administration of an equal volume of saline and the positive group (CTX group) was treated with 100 mg/kg Cytoxan by intraperitoneal injection at the fi rst day. After 2 wk of treatment, the anti-tumor activity was evaluated by tumor tissue weighing. The impact on immune organ was detected based on the thymus index, spleen index, phagocytic rate and phagocytic index. The protein expression of vascular endothelingrowth factor (VEGF) and basic fibroblast growth factor (bFGF) were detected by immunohistochemistry. The cell apoptotic rate was detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay. RESULTS: The A value in each group treated with Gecko after 72 h was reduced signif icantly in EC9706 and in EC1. The tumor weight in each group of Gecko was decreased signifi cantly (1.087 ± 0.249 vs 2.167 ± 0.592; 1.021 ± 0.288 vs 2.167 ± 0.592; 1.234 ± 0.331 vs 2.167 ± 0.592; P < 0.01, respectively). However, the thymus index and Spleen index of mice in Gecko groups had no significant difference compared with the NS group. The immunoreactive score of VEGF and bFGF protein expression of each Gecko group by immunohistochemical staining were lowered signifi cantly. The apoptosis index (AI) of each group was incr

关键词： Chinese medicine Gecko Human esophagealcarcinoma cells S180 sarcoma of mouse Vascularendothelin growth factor Basic fibroblast: growth factor Apoptosis

Status and Trends of GAP Base Construction of Chinese Materia Medica in Guangdong Province

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Chinese Herbal Medicines 2012年第1期4卷 33-42页

作者： Ping DING Xiao-ping LAI Hong-hua XU Qin DU jian-gang wang Ge YING Hui-jun LIAO Lei DAI Yan-hua SHAO School of Chinese Materia Medica Guangzhou University of Chinese Medicine Guangzhou 510405 China

It is one of the key points for modernization and internationalization of traditional Chinese medicines to construct the Good Agricultural Practice (GAP) base of Chinese materia medica (CMM). GAP helps to minimize contamination and improve the quality of CMM during the plantation and the production of Chinese crude drugs. In this article, the status and development of CMM production bases of GAP in Guangdong Province, China, are presented. The suggestions upon the problems during the development of GAP for Chinese crude drugs are also provided.

关键词： Chinese materia medica Good Agricultural Practice base Guangdong modernization and internationalization traditional Chinese medicines