Gecko crude peptides inhibit migration and lymphangiogenesis by down regulating the expression of VEGF-C in human hepatocellular carcinoma cells and human lymphatic endothelial cells

作     者：Meng-li GUO Cai-e WANG Yi-meng DUAN Jian-gang WANG 

作者机构：Medical CollegeHenan University of Science and Technology The First Affiliated Hospitaland College of Clinical Medicine of Henan University of Science and Technology 

出 版 物：《中国药理学与毒理学杂志》 (Chinese Journal of Pharmacology and Toxicology)

年 卷 期：2017年第31卷第10期

页      面：958-959页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by Medical Science and Technology Research Project of Henan Province(142102310031) 

主　　题：gecko crude peptides hepatic carcinoma vascular endothelial growth factor-C RNA interference(RNAi) lymphangiogenesis 

摘      要：OBJECTIVE To explore the role of gecko crude peptides(GCPs)in the proliferation,apoptosis,migration and lymphangiogenesis of human hepatocellular carcinoma cells(Hep G2)and human lymphaticendothelial cells(HLECs)in *** The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay was used to evaluate the anti-proliferative effect of GCPs and si RNA-VEGF-C on Hep G2 cells,Hoechst 33258 staining and flow cytometry were performed to analyze cycle and *** migration and invasion ability of cells were assayed by transwell chamber experiment and wound-healing *** protein and m RNA expressions of vascular endothelial growth factor-C(VEGF-C)and CXC chemokine receptor-4(CXCR4)were detected by q-PCR,immunofluorescence,Western *** protein expressions of the extracellular signal regulated kinase(ERKI/2),c-Jun N-terminal kinase(JNK),p38-mitogen activated protein kinases(p38 MAPK),serine/threonine kinase(Akt)and phosphatidylinositol-3-kinase(PI3K)were detected by western *** anti-lymphangiogenesis effect of GCPs on the HLECs was analyzed using an in vitro tube-formation *** protein and m RNA expressions of vascular endothelial growth factor receptor-3(VEGFR-3)and stromal cell-derived factor-1(SDF-1)were detected by q-PCR,Western *** GCPs and si RNA-VEGF-C inhibited Hep G2 proliferation,invasion and migration,and the most obvious inhibitory effect was both synergistic ***,GCPs suppressed HLECs proliferation,migration and tubelike structure formationin a dose-dependent manner,and had inhibitory effect of tumor-induced lymphangiogenesis in ***,we found that GCPs and si RNA-VEGF-C decreased the expressions of MMP-2,MMP-9,VEGF-C,CXCR4,phospho-ERK1/2,phospho-P38,phospho-JNK and PI3K in Hep G2 ***,GCPs had a dose-dependent depressive effecton the expressions of VEGFR-3,SDF-1 in *** The low expression of VEGF-C mediated by si RNA-VEGF-C and GCPs inhibit tumor proliferation,invas