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Cytogenetic characterization and description of an X_1X_1X_2X_2/X_1X_2Y sex chromosome system in Collichthys lucidus(Richardson,1844)

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Acta Oceanologica Sinica 2018年第4期37卷 34-39页

作者： zhang Shoukang ZHEnG jiao zhang j.ng WAnG Zhiyong WAnG Yilei CAI Mingyi The Key Laboratory of Healthy Mariculture for the East China Sea Ministry of Agriculture Fisheries College Jimei University

The chromosomes of spinyhead croaker Collichthys lucidus（Richardson,1844） were characterized for the first time by fluorescence staining,self genomic in situ hybridization（self-GISH）,and multicolor fluorescence in situ hybridization（FISH） with 18 SrDnA,5 SrDnA and telomeric sequence *** female karyotype has exclusively 24 pairs of acrocentric chromosomes（2 n=48 a,nF=48）,while the male one consists of 22 pairs of acrocentric chromosomes,2 monosomic acrocentric chromosomes and a metacentric chromosome（2 n=1 m＋46 a,nF=48）.The difference between female and male karyotypes indicates the presence of a sex chromosome of X1 X1 X2 X2/X1 X2 Y type,where Y is the unique metacentric chromosome in the male *** revealed by FISH,5 S r DnA and 18 S r DnA sites were mapped at syntenic position of the largest acrocentric chromosome（X_1）,and the short arms of the Y chromosome as *** X_1-chromosome specific interstitial telomeric signal（ITS） was detected overlapping the 5 S r DnA *** addition,self-GISH revealed that the repetitive DnAs accumulated on all the putative sex *** fusion accompanied by a partial deletion in the ancestral karyotype（2 n=48 a） is hypothesized for the origin of such multiple sex chromosome *** present study,as the first description of differentiated sex chromosome in family Sciaenidae,will give clues to the studies on the sex chromosome of other Sciaenids.

关键词： Collichthys lucidus karyotype sex chromosome rDnA fluorescence in situ hybridization interstitial telomeric signal (ITS)

Effect of Pressure on Absorption Spectra of Lycopene in n-Hexane and CS2 Solvents

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Chinese Physics Letters 2010年第1期27卷 108-110页

作者：张伟刘伟龙郑植仁霍明明李艾华杨彬 Center for Condensed Matter Science and Technology Department of Physics Harbin Institute of Technology Harbin 150001

The absorption spectra of lycopene in n-hexane and CS2 are measured under high pressure and the results are compared with β-carotene. In the lower pressure range, the deviation from the linear dependence on the Bayliss parameter （BP） for β-carotene is more visible than that for lycopene. With the further increase of the solvent BP, the 0-0 bands of lycopene and β-carotene red shift at almost the same rate in n-hexane; however, the 0-0 band of lycopene red shifts slower than that of β-carotene in CS2. The origins of these diversities are discussed taking into account the dispersion interactions and structures of solute and solvent molecules.

关键词： ABSORPTIOn spectra LYCOPEnE HEXAnE CARBOn disulfide SOLVEnTS HIGH pressure (Science) DISPERSIOn

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基础振动下直动式溢流阀的阀口泄漏分析

浙江大学学报（工学版） 2015年第6期49卷 1160-1165,1172页

作者：王蔚坪张怀亮章国亮中南大学机电工程学院湖南长沙410083 中南大学高性能复杂制造国家重点实验室湖南长沙410083

针对硬岩掘进机(TBM)在掘进过程中产生的振动影响液压元件内泄漏的问题,分析基础振动及结构参数对溢流阀阀口泄漏量的影响规律.以安装在TBM基础上的直动式溢流阀为研究对象,建立基础振动下溢流阀的阀口泄漏量数学模型.通过计算基础振动... 详细信息

针对硬岩掘进机(TBM)在掘进过程中产生的振动影响液压元件内泄漏的问题,分析基础振动及结构参数对溢流阀阀口泄漏量的影响规律.以安装在TBM基础上的直动式溢流阀为研究对象,建立基础振动下溢流阀的阀口泄漏量数学模型.通过计算基础振动引起的溢流阀阀口泄漏量,确定溢流阀发生阀口泄漏时的临界入口压力.结果表明:阀口泄漏量随着基础振动振幅及频率的增大而近似线性增大;当弹簧刚度小于1.3×105 n/m时,阀口泄漏量随着弹簧刚度的增大而增大;当弹簧刚度为1.3×105 n/m时,阀口泄漏量达到最大值;减小弹簧刚度和阀芯质量可以显著减少因基础振动引起的阀口泄漏故障,从而提高溢流阀的工作稳定性.

关键词：直动式溢流阀基础振动阀口泄漏硬岩掘进机(TBM) 弹簧刚度阀芯质量

Evaluation of the Effects of Standard Rescue Procedure on Severe Trauma Treatment in China

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Chinese Medical journal 2015年第10期128卷 1301-1305页

作者： Xiao-Feng Yin Tian-Bing Wang Pei-Xun zhang Yu-Hui Kou Dian-Ying zhang Kai Yu De-Cheng Lyu Mao-Zheng Liu Dong-Sheng Zhou Peng zhang jue-Hua j.ng wei-wei Ge Li-Ying Cao Guo-Sheng Wang Shao-jie Deng Weng-Hua Liu Mao zhang Yong-An Xu Kun zhang Bing Li wei Wang Zhong-Li Gao Cheng-La Yi Bao-Guo jiang Department of Trauma and Orthopeadics Peking University People's HospitalBeijing 100044China Peking University Traffic Medical Center Beijing 100191China Peking University Traffic Medical Center Beijing 100191 China Department of Orthopedics Tianjin Fifth Medicine Center Tianjin 300450 China Department of Orthopedics The Second Hospital of Dalian Medical University Dalian Liaoning 116023 China Department of Orthopedics Shandong Province Hospital Jinan Shandong 250021 China Department of Orthopedics The Second Hospital of Anhui Medical University Hefei Anhui 230601 China Department of General Surgery Kailuan General Hospital Tangshan Hebei 063000 China Department of Orthopedics People's Hospital of Bao'an District of Shenzhen Shenzhen Guangdong 518100 China Department of Emergency The Second Hospital of Zhejiang Medical University Hangzhou Zhejiang 310009 China Department of Orthopedics Xran Honghui Hospital Xran Shaanxi 710000 China Department of Orthopedics Liuzhou Worker's Hospital Liuzhou Guangxi 545005 China Department of Orthopedics The Center Hospital of Jingzhou City Jinzhou Liaoning 121001 China Department of Orthopedics The Third Hospital of Jilin University Changchun Jilin 130021 China Department of Orthopedics Tongji Hospital of Tongji University School of Medicine Wuhan Hubei 430030 China

Background：This study aimed to evaluate the effects of standard rescue procedure （SRP） in improving severe trauma treatments in ***：This study was conducted in 12 hospitals located in geographically and industrially different cities in China.A standard procedure on severe trauma rescue was established as a general rule for staff training and patient treatment.A regional network （system） efficiently integrating prehospital rescue,emergency room treatments,and hospital specialist treatments was built under the rule for information sharing and improving severe trauma *** outcomes were compared between before and I year after the implementation of the ***：The outcomes of a total of 74,615 and 12,051 trauma cases were collected from 12 hospitals before and after the implementation of the *** of the SRP led to efficient cooperation and information sharing of different treatment *** emergency response time,prehospital transit time,emergency rescue time,consultation call time,and mortality rate of patients were 24.24 ± 4.32 min,45.69 ± 3.89 min,6.38 ± 1.05 min,17.53 ± 0.72 min,and 33.82% ± 3.87% （n =441）,respectively,before the implementation of the standardization and significantly reduced to 10.11 ± 3.21 min,22.39 ± 4.32 min,3.26 ± 0.89 min,3.45 ± 0.45 min,and 20.49% ± 3.11%,separately （n =495,P 〈 0.05） after ***：Staff training and SRP can significantly improve the efficiency of severe trauma treatments in China.

关键词： China Procedure Standard Trauma Treatment

L-精氨酸-一氧化氮途径对病理性心肌肥厚形成的抑制作用及相关机制

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第二军医大学学报 2008年第2期29卷 177-183页

作者：杨涛张伟张雷河北医科大学组织学与胚胎学教研室石家庄050017 河北医科大学基础课部药理教研室石家庄050091

目的:从在体和离体水平分别探讨L-精氨酸对病理性心肌肥厚的影响及相关机制。方法:(1)在体水平:将大鼠分为假手术组、手术组、L-精氨酸(L-arginine,L-Arg)组和L-硝基精氨酸甲酯(n-nitro-L-arginine methy lester,L-nAME)组(L-Arg+L-nAME... 详细信息

目的:从在体和离体水平分别探讨L-精氨酸对病理性心肌肥厚的影响及相关机制。方法:(1)在体水平:将大鼠分为假手术组、手术组、L-精氨酸(L-arginine,L-Arg)组和L-硝基精氨酸甲酯(n-nitro-L-arginine methy lester,L-nAME)组(L-Arg+L-nAME组),利用腹主动脉缩窄制作大鼠模型。检测心肌内蛋白合成总量与心率、血压;比色法检测心肌内一氧化氮(nO)含量、放射免疫法检测心肌AT Ⅱ含量,RT-PCR检测心肌内血管紧张素转换酶(angiotensin converting enzyme,ACE)mRnA的表达水平。(2)离体水平:将原代培养的心肌细胞分为对照组、血管紧张素Ⅱ(AngiotensinⅡ,ATⅡ)处理组、L-Arg处理组(AT Ⅱ+L-Arg)及L-nAME处理组(ATⅡ+L-Arg+L-nAME)。RT-PCR检测原代培养心肌细胞诱导型一氧化氮合酶(inducible nO synthase,inOS)、ATⅡ的1型受体(AT Ⅱreceptor type 1,ATR1)和2型受体(ATⅡ receptor type 2,ATR2)mRnA的表达水平;Western印迹法检测原代培养心肌细胞inOS蛋白表达水平。结果:(1)L-Arg提高了腹主动脉缩窄大鼠心肌内nO含量,降低了心肌ATⅡ含量、心肌蛋白合成总量、左心室质量/体质量比值以及ACE mRnA的表达量;L-nAME可消除L-Arg的上述作用;(2)在离体条件下,与ATⅡ组相比,L-Arg处理组心肌细胞nO含量、inOS mRnA及蛋白水平的表达量均有提高,ATR1 mRnA表达水平下降;L-nAME处理组与ATⅡ处理组就上述指标相比无显著性差异;各组细胞ATR2 mRnA表达水平无明显差异;(3)多元逐步回归分析显示心肌内蛋白合成总量与血压水平、心率之间均不存在回归关系,而心肌nO和ATⅡ含量则可作为心肌内蛋白合成总量的预测因子;(4)心肌内ATⅡ含量、ACE mRnA表达量及心肌细胞ATR1 mRnA表达量均与nO含量之间存在线形负相关关系。结论:(1)心肌肥厚的形成与心肌内nO、ATⅡ含量密切相关;(2)L-Arg通过增强心肌inOS表达,致nO生成增加。nO可减少心肌内ATⅡ生成,并通过调控心肌ACE及ATR1表达来抑制病理性心肌肥厚的形成;(3)ATR2并未参与上述过程。

关键词： L-精氨酸一氧化氮诱导型一氧化氮合酶血管紧张素Ⅱ 心肌肥厚

加味香砂六君子汤调控高脂血症小鼠apoA-Ⅰ改善内质网应激的作用机制研究

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北京中医药大学学报 2024年第9期47卷 1236-1246页

作者：张琦隋国媛宋囡王杰刘玉蔡浩冉贾连群辽宁中医药大学中医药创新工程技术中心脏象理论及应用教育部重点实验室沈阳110847 辽宁中医药大学中西医结合学院

目的探讨加味香砂六君子汤调控小鼠载脂蛋白A-Ⅰ(apoA-Ⅰ)改善内质网应激调节糖脂代谢防治血脂异常的机制。方法将野生型C57BL/6j小鼠根据随机数字表法分为野生组、野生高脂组、野生中药干预组,将apoA-Ⅰ基因敲除小鼠根据随机数字表法... 详细信息

目的探讨加味香砂六君子汤调控小鼠载脂蛋白A-Ⅰ(apoA-Ⅰ)改善内质网应激调节糖脂代谢防治血脂异常的机制。方法将野生型C57BL/6j小鼠根据随机数字表法分为野生组、野生高脂组、野生中药干预组,将apoA-Ⅰ基因敲除小鼠根据随机数字表法分为敲除组、敲除高脂组、敲除中药干预组,每组10只。除野生组和敲除组正常饮食喂养外,各组小鼠采用D12492高脂饲料进行高脂饮食喂饲,建立高脂血症小鼠模型。于第9周开始野生中药干预组和敲除中药干预组小鼠每天给予加味香砂六君子汤(23.66 g/kg)灌胃给药,4周后,收集小鼠血清及肝脏组织用于后续检测。全自动生化分析仪检测血脂水平;ELISA法检测血清空腹血糖(FBG)及胰岛素(InS)水平,计算胰岛素抵抗(HOMA-IR)指数;HE染色观察肝脏病理变化;油红O染色观察肝脏脂质沉积情况;微板法检测肝脏组织甘油三酯(TG)水平;实时荧光PCR法检测肝脏组织apoA-Ⅰ、葡萄糖调节蛋白78(GRP78)、固醇调节元件结合蛋白-1c(SREBP-1c)、乙酰辅酶A羧化酶1(ACC1)、脂肪酸合酶(FASn)mRnA表达,WES全自动蛋白表达分析系统检测肝脏组织apoA-Ⅰ、GRP78、肌醇需求酶1(IRE1)、磷酸化IREI(p-IRE1)、c-j.n氨基末端激酶(jnK)、磷酸化jnK(p-jnK)、胰岛素受体底物1(IRS1)、磷酸化IRS1(p-IRS1)、蛋白激酶B(Akt)、p-Akt、SREBP-1c、ACC1、FASn蛋白表达。结果野生高脂组相较于野生组血清中血脂、FBG、InS水平及HOMA-IR指数升高(PnA及蛋白表达降低,GRP78、SREBP-1c、ACC1、FASn mRnA表达升高,GRP78、SREBP-1c、ACC1、FASn蛋白水平及IRE1、jnK、IRS1、Akt磷酸化程度升高(PnS水平及HOMA-IR指数降低(PnA及蛋白表达升高,GRP78、SREBP-1c、ACC1、FASn mRnA表达水平降低,GRP78、SREBP-1c、ACC1、FASn蛋白水平及IRE1、jnK、IRS1、Akt磷酸化程度降低(Pn mRnA表达水平升高,SREBP-1c、ACC1蛋白水平及IRE1、jnK、IRS1、Akt磷酸化程度升高(PnS水平及HOMA-IR指数升高,HDL-C水平降低(Pn mRnA表达水平升高,GRP78、SREBP-1c、ACC1、FASn蛋白水平及IRE1、jnK、IRS1、Akt磷酸化程度升高(P<0.05)。结论加味香砂六君子汤通过调控apoA-Ⅰ缓解内质网应激,进而改善肝脏糖脂代谢紊乱。

关键词：高脂血症载脂蛋白A-Ⅰ 内质网应激糖脂代谢加味香砂六君子汤小鼠

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In situ electron-induced reduction of nOx via CnTs activated by DBD at low temperature

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Frontiers of Environmental Science & Engineering 2020年第2期14卷 87-96页

作者： weixuan Zhao Liping Lian Xingpeng j.n Renxi zhang Gang Luo Huiqi Hou Shanping Chen Ruina zhang Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention(LAP3) Institute of Environmental ScienceFudan UniversityShanghai 200433China national Engineering Laboratory for VOCs Pollution Control Material&Technology University of Chinese Academy of SciencesBeijing 100049China Shanghai Institute for Design&Research on Environmental Engineering Shanghai 200232China

In this study,a new in situ electron-induced process is presented with carbon nanotubes(CnTs)as a reduction agent activated by dielectric barrier discharge(DBD)for nitrogen oxide(nOx)abatement at low temperature(ngle DBD system and a DBD system with activated carbon(DBD-AC),a DBD system with carbon nanotubes(DBD-CnT)showed a significant promotion of nOx removal efficiency and n2 *** the 02 content was 10%,the nOv conversion and n2 selectivity in the DBD-CnT system still reached 64.9%and 81.9%at a specific input energy(SIE)of 1424 j^L,and these values decreased to 16.8%,31.9%and 43.2%,62.3%in the single DBD system and the DBD-AC system,respectively.X-ray photoelectron spectroscopy(XPS)and scanning electron microscopy(SEM)were utilized to investigate surface changes in the CnTs after activation by DBD to explore the nO.t reduction abatement mechanism of this new ***,the outlet gas components were also observed via Fourier transform infrared spectroscopy(FTIR)to help reveal the nOr reduction *** results verified that carbon atoms excited by DBD and the structure of CnTs contributed to the synergistic activity of the DBD-CnT *** new denOx process was accomplished through in situ heterogenetic reduction reactions between the nOx and carbon atoms activated by the plasma on the *** addition,further results indicated that the new denOv process exhibited acceptable S02 tolerance and water resistance.

关键词： Dielectric barrier discharge nOx abatement In situ electron-induced reduction Carbon nanotubes

Enzyme Activities and Microbial Communities in Subtropical Forest Soil Aggregates to Ammonium and nitrate-nitrogen Additions

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journal of Resources and Ecology 2017年第3期8卷 258-267页

作者： wei Yan WAnG Zhongqiang zhang Xinyu YAnG Hao LIU Xiyu LIU Wenj.ng northeast normal University College of Geographic Science Changchun 130024 China Key Laboratory of Ecosystem network Observation and Modeling Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences Beij ing 100101 China College of Resources and Environment. University of Chinese Academy of Sciences Beijing 100190 China

A laboratory incubation experiment was established to examine the impacts of nitrate and ammonium nitrogen additions on soil microbial attributes of a subtropical Pinus elliottii forest ecosystem in southern China. Soils were subjected to three different treatments： the control with no nitrogen addition （CK）, the ammonium nitrogen addition （nH4^＋-n）, and the nitrate nitrogen addition （nO23^-n）. Samples from bulk and two different size fractions （macroaggregate （〉250 pm） and microaggregate （53-250 μm）） were analyzed for soil properties, enzyme activities and microbial communities on day 7 and 15 of the incubation. Our study demonstrated that nH4^＋-n had a 9rearer influence on soil microbial activities than nO3-n. nH4^＋-n additions resulted in significant increases in 13-1,4-glucosidase （βG） and β-1,4-n-acetyl glucosaminidase （nAG） enzyme activities in bulk, macroaggregate and microag- gregate soils after 7 and 15 days incubation. nO3^-n additions only significantly increased in βG and nAG enzyme activities in bulk, macroaggregate soils after 7 and 15 days incubation, but not in microaggregate. All nH4^＋-n and nO3-n additions resulted in significant increases in gram-positive bacterial PLFAs in microaggregates. Only a significant correlation between soil nutrient contents and enzyme activities in macroaggregates was founded, which suggests that the soil aggregation structure played an important role in the determining enzyme activities.

关键词： nitrate nitrogen ammonium nitrogen soil aggregate enzyme activity phosphor-lipid fatty acid (PLFA).

骨科创伤急诊患者预后的影响因素分析及列线图模型的构建与验证

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南昌大学学报（医学版） 2024年第1期64卷 60-64页

作者：陈立娟耿欣姚立军魏立友李春江吴春云张艳唐山市第二医院急诊科河北唐山063015

目的分析骨科创伤急诊患者预后的影响因素,构建预后风险预测的列线图模型并对其进行验证。方法选取2020年1月至2022年3月在唐山市第二医院就诊的骨科创伤急诊患者312例。根据患者就诊后28 d是否存活分为存活组(n=260)和死亡组(n=52)。... 详细信息

目的分析骨科创伤急诊患者预后的影响因素,构建预后风险预测的列线图模型并对其进行验证。方法选取2020年1月至2022年3月在唐山市第二医院就诊的骨科创伤急诊患者312例。根据患者就诊后28 d是否存活分为存活组(n=260)和死亡组(n=52)。单因素分析2组一般资料、损伤严重程度评分(ISS)及血清学指标[纤维蛋白原(FIB)、D-二聚体(D-D)、血管生成素2(Ang2)、可溶性白细胞分化抗原14(sCD14)]水平;Logistic多因素回归分析筛选骨科创伤急诊患者预后的独立危险因素;基于获得的独立危险因素构建风险列线图模型,并验证模型的预测效能。结果2组创伤程度、创伤部位数量、失血性休克、现场急救情况、ISS评分及FIB、D-D、Ang2、sCD14水平比较,差异均有统计学意义(Png2≥2911.15 pg·mL^(-1)(OR=6.821)、sCD14≥4.77 pg·mL^(-1)(OR=9.347)均是骨科创伤急诊患者预后的独立危险因素(Pndex为0.978。结论创伤部位数量、失血性休克、ISS评分、Ang2、sCD14是骨科创伤急诊患者预后的影响因素,根据这些因素构建的列线图可较好地预测患者的预后。

关键词：骨科创伤急诊列线图模型预后影响因素

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