Enzyme Activities and Microbial Communities in Subtropical Forest Soil Aggregates to Ammonium and Nitrate-Nitrogen Additions

作     者：WEI Yan WANG Zhongqiang ZHANG Xinyu YANG Hao LIU Xiyu LIU Wenjing 魏艳;王忠强;张心昱;杨浩;刘希玉;刘文静

作者机构：Northeast Normal University College of Geographic Science Changchun 130024 China Key Laboratory of Ecosystem Network Observation and Modeling Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences Beij ing 100101 China College of Resources and Environment. University of Chinese Academy of Sciences Beijing 100190 China 

出 版 物：《Journal of Resources and Ecology》 (资源与生态学报（英文版）)

年 卷 期：2017年第8卷第3期

页      面：258-267页

核心收录：

学科分类：09[农学] 0903[农学-农业资源与环境] 090301[农学-土壤学] 

基　　金：National Natural Science Foundation of China(41571251,41571130043) Technology Innovation Program of Chinese Academy of Sciences(201604) 

主　　题：nitrate nitrogen ammonium nitrogen soil aggregate enzyme activity phosphor-lipid fatty acid (PLFA). 

摘      要：A laboratory incubation experiment was established to examine the impacts of nitrate and ammonium nitrogen additions on soil microbial attributes of a subtropical Pinus elliottii forest ecosystem in southern China. Soils were subjected to three different treatments： the control with no nitrogen addition （CK）, the ammonium nitrogen addition （NH4^＋-N）, and the nitrate nitrogen addition （NO23^-N）. Samples from bulk and two different size fractions （macroaggregate （〉250 pm） and microaggregate （53-250 μm）） were analyzed for soil properties, enzyme activities and microbial communities on day 7 and 15 of the incubation. Our study demonstrated that NH4^＋-N had a 9rearer influence on soil microbial activities than NO3-N. NH4^＋-N additions resulted in significant increases in 13-1,4-glucosidase （βG） and β-1,4-N-acetyl glucosaminidase （NAG） enzyme activities in bulk, macroaggregate and microag- gregate soils after 7 and 15 days incubation. NO3^-N additions only significantly increased in βG and NAG enzyme activities in bulk, macroaggregate soils after 7 and 15 days incubation, but not in microaggregate. All NH4^＋-N and NO3-N additions resulted in significant increases in gram-positive bacterial PLFAs in microaggregates. Only a significant correlation between soil nutrient contents and enzyme activities in macroaggregates was founded, which suggests that the soil aggregation structure played an important role in the determining enzyme activities.