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浸润细胞、相关的细胞因子和趋化因子受体在皮肌炎皮损中的表达

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世界核心医学期刊文摘（皮肤病学分册） 2005年第3期1卷 51-52页

作者： Caproni m. Torchia D. Cardinali C. P. Fabbri 崔荣 Clinica Dermatologica II Via della Pergola 58- 60 50121 Firenze Italy

Background: There have been only two reports on immunophenotypic characteriza tion in the cutaneous lesions of dermatomyositis (Dm) that emphasize the importa nce of the infiltrating CD4+ T lymphocytes. Objectives: To characterize the imm unophenotype of the cells that infiltrate the lesional skin of Dm and to evaluat e the possible T-helper (Th) polarization Th1/Th2 through detection of specifi c cytokines, chemokine receptors and markers of cellular activation. methods: Sk in biopsy specimens derived from pathognomonic lesions (Gottron’s papules and Gottron’s sign) of eight patients withDmwere immunostainedwith a large panel o fmonoclonal antibodies to CD3, CD4, CD8, myeloperoxidase(mPO), eosinophil cation ic protein, tryptase, CD40, CD40 ligand (CD40L), hla-DR, interleukin (IL)- 2, IL- 4, IL- 5, IL- 13, interferon-γ , tumour necrosis factor-α , recept or 3 for CXC chemokines (CXCR3) and receptor 3 for CC chemokines, using the alka line phosphatase-antialkaline phosphatase method. Control specimens were obtai ned from five healthy subjects and from six patients with discoid lupus erythema tosus. Results: Activated CD4+ Th lymphocytes (hla-DR+ CD40L+ ) were the principal infiltrating cells in the lesional skin of Dm; the CD4/CD8 ratio was a pproximately 2· 5. A mixed Th1/Th2 profile and higher Th1 cytokine production t ogether with significant staining for CXCR3 were detected. Neutrophil granulocyt es were the second most abundant population; eosinophil granulocytes were very p oorly represented. Conclusions: Activated CD4+ T cells presumably mediate the main pathogenetic mechanisms in pathognomonic skin lesions. The interaction betw een CD40 andCD40L could be an important mecha nismof cellular activation in cutaneous immune-mediated inflammation by ind uction of secretion of proinflammatory cytokines and chemokines. Neither Th1 nor Th2 clear polarization was found, although there was a slight Th1 prevalence. T here was a significant quantity of mPO+ cells (neutrop

关键词：皮肌炎浸润细胞趋化因子受体单克隆抗体检测髓过氧化物酶免疫表型碱性磷酸酶类胰蛋白酶活性作用干扰素γ

Hsp70-1ike protein 1 fusion protein enhances induction of carcinoembryonic antigen-specific CD^8＋ CTL response by dendritic cell vaccine

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第二军医大学学报 2006年第1期27卷 100-100页

作者： Wu Y Wan T Zhou X Wang B Yang F Li N Chen G Dai S Liu S Zhang m Cao X Institute of Immunology Second Military Medical University Shanghai 200133 China

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上海地区汉族群体的hla-DP基因分型

中华医学遗传学杂志 1993年第3期10卷 131-134页

作者：范丽安张锦锋葛瑜姚芳娟杨珏琴陈仁彪 m.Sirikong R.Vongrathna P.Longta D.Chandanayimgyong 上海第二医科大学上海市免疫学研究所免疫遗传研究室 200025 Department of transfortion medicine Siriraj Hospital mahidol University Bangkok Thailand

对上海地区48名汉族个体的基因组DNA用聚合酶链反应(PCR)扩增及对寡核苷酸探针(SSO)进行斑点杂交的方法研究了hla-DPA1及DPB1基因分布情况。共检出DPB1等位基因9个,以0501等位基因占优势,检出率为64.6%,其次为0201及0401等位基因,检出... 详细信息

对上海地区48名汉族个体的基因组DNA用聚合酶链反应(PCR)扩增及对寡核苷酸探针(SSO)进行斑点杂交的方法研究了hla-DPA1及DPB1基因分布情况。共检出DPB1等位基因9个,以0501等位基因占优势,检出率为64.6%,其次为0201及0401等位基因,检出率分别为27.1%和25.0%。结果表明,上海地区汉族群体的DPB1分布与白种人有明显不同,揭示不同人种有其自己的主要等位基因。

关键词：人白细胞抗原 DP 基因分型多态性

hla-DQ and-DR alleles in patients with SLE:A tertiary hospital-based case–control study in Bangladesh

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Rheumatology & Autoimmunity 2023年第1期3卷 43-49页

作者： muhammad S.A.Khan Shamim Ahmed Iftekhar H.Bandhan Farhana Zaman mdKamrul H.Sajib Shirin Tarafder minhaj R.Choudhury Syed A.Haq mohammad m.Zaman Sheikh Hasina National Institute of Burn and Plastic Surgery DhakaBangladesh Department of Rheumatology Bangabandhu Sheikh Mujib Medical UniversityDhakaBangladesh Upazilla Health Complex BancharampurB-BariaBangladesh Department of Community medicine Universal Medical CollegeDhakaBangladesh Department of Rheumatology Dhaka Medical College HospitalDhakaBangladesh Green Life Center for Rheumatic Care and Research DhakaBangladesh World Health Organization DhakaBangladesh

Background:Systemic lupus erythematosus(SLE)is a systemic autoimmune disease characterized by the involvement of multiple organs and autoantibody *** study aimed to determine the association of human leukocyte antigen(hla)-DQ and-DR alleles with SLE at a tertiary hospital in ***:This case-control study was conducted in the Department of Rheumatology,Bangabandhu Sheikh mujib medical University(BSmmU),Dhaka,*** patients who fulfilled the 1997 revised American College of Rheumatology classification criteria for SLE were considered cases(n=41).The controls(n=32)were selected from the hospital staff of BSmmU,including doctors and nurses who did not suffer from any known rheumatic diseases,and there was no such disease in their first-degree *** chain reaction using a sequence-specific primer(PCRSSP)in a low-resolution typing method was carried out on blood samples collected for hla-DQ and-DR allele ***:The cases and controls had comparable mean ages(31 vs.32 years),and most cases were female(95.1%vs.34.4%).The most frequent clinical manifestations were mucocutaneous(85.4%),followed by musculoskeletal(70.7%)and constitutional symptoms(58.5%).The cases had a significantly higher frequency of hla-DR2 allele compared with healthy controls(70.7%vs.43.8%;odds ratio:3.1;95%confidence interval:1.2-8.2).In addition,hlaDQ6 allele was found to have a significant association with mucocutaneous manifestations of the cases(88.9%,P=0.03).Conclusions:Bangladeshi adults having hla-DR2 may be more susceptible to ***-DQ6 allele might be associated with mucocutaneous manifestations of SLE.

关键词： alleles Bangladesh hla-DQ hla-DR systemic lupus erythematosus

多发性硬化症与自身免疫性疾病:意大利东北部人群流行病学与hla-DR之间的联系

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世界核心医学期刊文摘(神经病学分册) 2006年第10期 25-26页

作者： Laroni A. Calabrese m. Perini P. P. Gallo 牛亚利 multiple Sclerosis Centre of the Veneto Region First Neurology Clinic - Dept. of Neurosciences University of Padova Via Giustiniani 5 35128 - Padova Italy Dr.

An autoimmune background is thought to characterize the families of multiple sclerosis (mS) patients, but disease patterns and hla- DR association seem to vary considerably among different ethnic groups. We investigated the prevalence of autoimmune diseases in 245 mS patients and 245 age- and sex- matched normal controls (NC), originating from and living in North- east Italy, and their first degree relatives, using a casecontrol method. Further, hla- DRB1 expression was analysed in mS and NC. The following significant findings were observed: 1) a significant excess of autoimmunity in first- degree relatives of mS patients (p = 0.000), 2) an association of mS with Type 1 diabetes mellitus (T1Dm) (p = 0.02), 3) an increase in DR4 expression (namely DRB1 0401) in mS patients from families with multiple autoimmune pathology compared with reference mS patients (p=0.02) and NC (p=0.01).We conclude that the risk of autoimmune disease is higher in first- degree relatives of mS patients and that disease association and hla- DR expression in North- east Italy differs from other geographic regions of Europe.

关键词：自身免疫性疾病一级亲属患者家族自身免疫病家系多发性硬化症 hla-DR 南欧意大利

分离人杀伤细胞抑制受体NKAT2特异的单链抗体

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暨南大学学报（自然科学与医学版） 1998年第6期19卷 51-52页

作者：乔旭刚 Colon.,m 暨南大学医学院血液病研究室

人自然杀伤细胞表面的抑制受体（ＫＩＲ），是与表达在其他细胞表面的相应ＨＬＡＩ类分子相互作用的。许多ＫＩＲ受体已被克隆，小部分量的单克隆抗体也已产生。然而为区分不同位点及其等位基因的产物，并精确阐明其功能，需要更多的研... 详细信息

人自然杀伤细胞表面的抑制受体（ＫＩＲ），是与表达在其他细胞表面的相应ＨＬＡＩ类分子相互作用的。许多ＫＩＲ受体已被克隆，小部分量的单克隆抗体也已产生。然而为区分不同位点及其等位基因的产物，并精确阐明其功能，需要更多的研究试剂。本研究探讨用噬菌体抗体库，分离ＫＩＲ单链抗体（ｓｃＦｖ）的可行性。方法：ＮＫＡＴ２－ＩｇＧ１为一ＫＩＲ，与人ＩｇＧ１Ｆｃ以融合蛋白形成表达而获得。噬菌体抗体库为“Ｎｉｓｉｍ”抗体库。抗体库ＮＫＡＴ２－ＩｇＧ１包被的免疫管５次筛选。ｓｃＦｖ由特异的噬菌体感染细菌，诱导而得。筛选效应经噬体菌体的菌落形成单位及ＤＮＡ酶切图谱检测。筛选的克隆进行ＤＮＡ序列分析。ｓｃＦｖ的特异性及免疫学特征由ＥＬＩＳＡ、聚丙烯胺凝胶电泳及免疫印迹分析。细胞结合实验由流式细胞仪测试。结果：５次筛选后，ＮＫＡＴ２特异的噬菌体达到２００倍的富集，ＤＮＡ酶切图谱也显示某种特定消化类型的富集。经ＥＬＩＳＡ分析，结果显示３７个克隆与ＮＫＡＴ２反应呈强阳性。这些克隆与ＮＫＡＴ１－ＩｇＧ１、ＮＫＡＴ３－ＩｇＧ１、ＮＫＡＴ４－ＩｇＧ１、人ＩｇＧ及其他非相关蛋白的反应均为阴性。其中一个克隆（ｓｃＨＵＢ１）经流式细胞仪进一步测试，显示?

关键词：杀伤细胞分离抗体抑制受体 NKAT2 单链抗体

我国西双版纳傣族群体hlaⅡ类基因的寡核苷酸分型

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中国免疫学杂志 1992年第5期8卷 287-290页

作者：范丽安杨玉琴董建中姚芳娟许玲娣陈仁彪 m.Sirikong P.Longa R.Vongrathna D.Chandanayingyong 上海市二医科大学上海市免疫学研究所上海200025 Department of Transfusion medicine Siriraj HospitalMahidol UniversityBangkokThailand

我们对73名西双版纳傣族正常健康个体用第11届国际组织相容性专题讨论会的方法进行了hla Ⅱ类基因的 PCR/SSO 分型。结果表明傣族的 hla Ⅱ类等位基因频率分布在与中国南方人群相似以外尚有其独特性.共捡出 DRB1 等位基因20种,其中基因... 详细信息

我们对73名西双版纳傣族正常健康个体用第11届国际组织相容性专题讨论会的方法进行了hla Ⅱ类基因的 PCR/SSO 分型。结果表明傣族的 hla Ⅱ类等位基因频率分布在与中国南方人群相似以外尚有其独特性.共捡出 DRB1 等位基因20种,其中基因频率超过0.10的依次为1602、0901、1502、1401及1202,未发现01、08及1001等位基因。DRB3仅捡出0202及0301,DRB5仅捡出0101和0102。DQA1共捡出6种等位基因以0102、0101、03频率为最高。以0502为最高频率的 DQB1共捡出9种等位基因。绝大多数个体(91.8%)均具有 DPA1、0201等位基因、与目前所报道的东方人材料相一致,DPB1 0501频率最高,其次为0202、0201与1301。各位点均呈 Handy-WeinKeng 平衡.

关键词：傣族 PCR-SSO分型 hlaⅡ类基因

中国患者患寻常型银屑病与PSORS1 C3和CDSN基因相关

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世界核心医学期刊文摘（皮肤病学分册） 2006年第12期2卷 7-8页

作者： Chang Y.T. Chou C.T. Shiao Y.m. 张路坤 Department of Dermatology National Yang-Ming University Taipei TaiwanChina

Background: Besides the hla-Cw0602 allele, the psoriasis susceptibility 1 candidate 3 (PSORS1C3) and corneodesmosin (CDSN) genes are two probable psoriasis susceptibility genes in the PSORS1 locus. The -79C, -26C and +246A alleles of the PSORS1C3 gene, the CDSN971T allele, CDSNTTC (619T-1236T-1243C) and CDSN5 (619T-1240G-1243C) are strongly associated with psoriasis in the caucasian population. Until now, no haplotype study of the PSORS1C3 and CDSN genes has been documented in Chinese patients with psoriasis vulgaris. Objectives: We aimed to determine whether genetic polymorphisms of the PSORS1C3 and CDSN genes were associated with an increased risk of psoriasis vulgaris in Chinese patients in Taiwan. methods: We investigated the PSORS1C3 and CDSN genes for disease association by direct sequencing in 178 patients with psoriasis vulgaris and 203 control subjects. Genotyping for hla-Cw0602, α-helix coiled-coil rod homologue (HCR) gene and single nucleotide polymorphism (SNP) n.9 was also carried out using a sequence-based typing method. Results: The PSORS1C3582A allele, an SNP in the 3-untranslated region of the PSORS1C3 gene, was a major psoriasis vulgaris susceptibility allele in the Chinese population, and the association was much stronger in patients with early-onset psoriasis vulgaris (22.3%vs. 6.9%, odds ratio = 3.87, Pc =0.0000 072). The frequencies of CDSNTTC and CDSN971T were also significantly increased in patients with early-onset psoriasis vulgaris. moreover, PSORS1C3582A, SNP n.9C,Cw0602 and HCRWWCC were in near complete linkage disequilibrium(LD) with each other; in contrast, the LD with the CDSN gene was not so strong. SNP n.9C-Cw0602-PSORS1C3582A-HCRWWCC was a major susceptibility haplotype in patients with early-onset psoriasis vulgaris (P mportant psoriasis susceptibility genes in Chinese patients with psoriasis vulgaris.

关键词：寻常型银屑病 PSORS1 C3 RS CDSN 患者中华人民共和国

妊娠早期血浆低hla-G蛋白水平预示先兆子痫

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世界核心医学期刊文摘（妇产科学分册） 2005年第11期1卷 10-11页

作者： Yie S.-m. Taylor R.N. Librach C. 朱晓明 790 Bay Street Toronto Ont. M5G 1N8 Canada Dr.

Objective: The aim of this study was to determine whether circulating hla-G levels, early in pregnancy, predict the subsequent development of preeclampsia (PE). Study design: Plasma samples, collected longitudinally during the first, second, and third trimesters, from 12 PE patients and 12 matched control patients were tested for hla-G protein using a validated sandwich ELISA. Results: First and second trimester hla-G levels in PE were significantly lower than in control patients (first trimester, 1.25 μ g/mL vs 1.95 μ g/mL, P = .029; second trimester, 1.11 μ g/mL vs 1.90 μ g/mL, P = .024). Conclusion: Our results indicate that hla-G levels in plasma from women who subsequently develop PE are lower than control patients, as early as the first trimester. This suggests that determination of circulating hla-G protein concentration may be useful as an early predictor for the development of PE.

关键词：妊娠早期 hla-G蛋白先兆子痫早期预测研究设计