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complexed amino acid minerals ***-glycinate chelated minerals:Impact on the performance of old laying hens

Animal Nutrition 2024年第1期16卷 395-408页

作者： Marcos J.B.Santos Maria c.M.M.Ludke Leandro M.Silva carlos B.V.Rabello Mércia R.Barros Fabiano S.costa clariana S.Santos Jamille S.S.wanderley Department of Animal Science Rural Federal University of PernambucoRecifePEBrazil Department of Veterinary Science Rural Federal University of PernambucoRecifePEBrazil

The present study was to evaluate the effect of trace minerals(Zn,Mn,and cu)from complexed amino acid minerals(ZMcAA)and bis-glycinate chelated minerals(ZMcGly)in laying hen diets on performance,internal and external egg quality,yolk mineral deposition,intestinal morphometry,and bone *** 78 to 98 weeks of age,400 White LSL-Lite strain laying hens were distributed in a randomized design with 4 treatments with 10 replicates per *** were distributed in a 2×2 factorial arrangement using either Zn,Mn,and cu of ZMcAA or ZMcGly source at 2 levels:low(20,20,and 3.5 mg/kg of Zn,Mn,and cu,respectively)or high(40,40,and 7 mg/kg of Zn,Mn,and cu,respectively).The analysis of variance was performed,and in cases where differences were observed,the means were compared using Tukey?s test(Pce and level of trace mineral supplementation had a significant impact on the performance of laying *** fed ZMcAA had higher egg production(P=0.01),egg weight(P=0.02),egg mass(P=0.01),and lower feed conversion ratio(P=0.05)compared to those fed *** ZMcAA supplementation showed higher albumen height(P=0.01),albumen weight(P=0.01),and eggshell thickness(Pcu(Pyolk was greater for hens received *** weight(P=0.04)and bone densitometry(PcAA *** the small intestine,ZMcAA resulted in longer villi(P=0.02)and shorter crypt depth(P=0.01)in the *** and ileum measurements were influenced by the level and source of trace minerals(Pying hens fed ZMcAA exhibited superior performance,egg quality,deposition of trace minerals in the egg yolk,and bone density compared to hens fed *** this study,older laying hens supplemented with ZMcAA at lower levels demonstrated adequate levels of supplementation.

关键词： Bis-glycinates Metal-amino acids Radiodensity Trace minerals

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2015年巴西寨卡病毒感染与小头畸形之间可能存在的关联

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上海预防医学 2017年第1期29卷 82-84页

作者： Lavinia Schuler-Faccini Erlane M.Ribeiro Ian M.L.Feitosa Dafne D.G.Horovitz Denise P.cavalcanti AndréPessoa Maria Juliana R.Doriqui Joao Ivanildo Neri Joao Monteiro de Pina Neto hector y.c.wanderley Mirlene cernach Antonette S.El-Husny Marcos V.S.Pone cassio L.c.Serao Maria Teresa V.Sanseverino 朱秋丽(译) 陆仁敏(校) Universidade Federal do Rio Grande do Sul Brazil Hospital Infantil Albert Sabin FortalezaCEBrazil Universidade Federal de Pernambuco Brazil Fundaqao Oswaldo cruz Rio de JaneiroBrazil University of campinas Sao PauloBrazil Hospital Infantil Juvencio Mattos MaranhaoBrazil Universidade Potiguar Rio Grande do NorteBrazil University of Sao Paulo Ribeirao PretoBrazil Secretaria de Estado da Saude do Espirito Santo Brazil Universidade Federal de Sao Paulo Brazil centro Universitario do Estado do Para Brazil Universidade do Estado do Rio de Janeiro Brazil Hospital de clinicas de Porto Alegre Brazil Brazilian Medical Genetics Society-Zika Embryopathy Task Force 不详

2015年初,通过伊蚊传播的寨卡病毒在登革热病毒流行地区巴西的东北部暴发,该病毒属于黄病毒属。2015年9月,寨卡病毒流行地区开始出现关于小头畸形患儿的报道,且报道数量呈现增加的趋势。与此同时,有2名妇女产前超声检查发现有胎儿畸形,... 详细信息

2015年初,通过伊蚊传播的寨卡病毒在登革热病毒流行地区巴西的东北部暴发,该病毒属于黄病毒属。2015年9月,寨卡病毒流行地区开始出现关于小头畸形患儿的报道,且报道数量呈现增加的趋势。与此同时,有2名妇女产前超声检查发现有胎儿畸形,在她们的羊水中也检测出寨卡病毒RNA。

关键词：小头畸形病毒感染报道数量登革热病毒产前超声检查胎儿畸形西寨黄病毒属病毒流行先天性异常

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Trypanosoma cruzi invasion in non-phagocytic cells:an ultrastructural study

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BIOcELL 2018年第3期42卷 105-108页

作者： Juan Agustín cUETO Emile SANTOS BARRIAS wanderley de SOUZA Patricia Silvia ROMANO IHEM Universidad Nacional de CuyoCONICETLaboratorio de Biología de Tripanosoma cruzi y de la célula hospedadoraMendozaArgentina Universidad Nacional de cuyo.Facultad de ciencias Médicas.Área de Biología celular y Molecular.Mendoza.Argentina Universidad Nacional de cuyo.Facultad de ciencias Médicas.Instituto de Fisiología.Mendoza.Argentina Laboratório de Ultraestrutura celular Hertha Meyer Instituto de Biofísica Carlos Chagas FilhoCentro de Ciência da SaúdeUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil Instituto Nacional de ciência e Tecnologia em Biologia Estrutural e Bioimagens e centro Nacional de Bioimagens-cENABIO Universidade Federal do Rio de JaneiroRio de JaneiroBrazil Instituto Nacional de Metrologia Qualidade e Tecnologia-InmetroRio de JaneiroBrazil

Trypanosoma cruzi is the causative agent of chagas *** parasite requires the intracellular niche in order to proliferate and disseminate the *** invasion,*** resides temporarily in an acidic vacuole which is lysed by a not well-understood *** electron microscopy was used to describe the process of *** escape from the parasitophorous vacuole over the *** HeLa(non-professional phagocytic cells)as host cell,we observed that recently internalized parasites reside in a membrane-bounded vacuole.A few hours later,the first sign of vacuole disruption appeared as membrane *** observation was followed by a progressive vacuole swelling as evidenced by an electron-lucent halo between the parasite and the vacuole ***,the vacuole membrane remnants reorganized as small vesicles that eventually disappeared from the vicinity of the ***,parasites reach the host cell cytosol where replication takes *** thorough ultrastructural description of this process set the base for a comprehensive understanding of the parasite-host cell interaction and,thus open the possibility of new therapeutic intervention strategies.

关键词： Parasitophorous vacuole Pathogen Parasites chagas disease Infectious diseases

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Ontogenetic niche specialization of the spider crab Libinia ferreirae associated with the medusa Lychnorhiza lucerna

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current Zoology 2022年第5期68卷 549-559页

作者： Geslaine Rafaela Lemos Gonçalves Milena Regina Wolf Mariana Antunes Felipe wanderley Amorim Maria Lucia Negreiros-Fransozo Antonio Leão castilho Group of Studies on crustacean Biology Ecology and Culture-NEBECCBiosciences InstituteSão Paulo State UniversityBotucatuSP 18618-689Brazil and Laboratory of Pollination Ecology and Interactions-LEPI Biosciences InstituteSão Paulo State UniversityBotucatuSP 18618-689Brazil

Symbiotic relationships in marine environments are not fixed and can change throughout the animal’s *** study investigated the ontogeny of symbiosis of the spider crab Libinia ferreirae with the host medusa Lychnorhiza *** described the type of relationship,the temporal correlation among species,and food *** than 50%of the sampled crabs were symbionts,most in early life *** highest number of crabs found in a single medusa was *** was observed throughout most of the year but was more evident in warm *** crab has many benefits in this relationship with a *** is the use of food resources captured by the medusa,primarily *** the crab steals the medusa’s food,it is a kleptoparasitic *** is a niche partition between symbiont and the free-living crabs as they occupy different habitats and use nonoverlapping food *** research reported that symbiosis first developed during the crab’s last larval phase(megalopa)when crab and medusa are in the same *** of the crab’s behavior shows that symbiosis occurs when the crab can grab to the medusa when the host touches the sea *** crab also took advantage of water currents,releasing itself from the substrate and then drifting toward the *** symbiotic relationship that crabs have with the medusa provides then with a nursery,food resources,shelter,dispersion,and decreased competition with free-living adult crabs,all essential for the crab’s survival.

关键词： behavior development kleptoparasitism megalopa niche segregation

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Green tea polyphenols inhibit testosterone production in rat Leydig cells

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Asian Journal of Andrology 2009年第3期11卷 362-370页

作者： Marina S. Figueiroa Juliany S. B. cesar Vieira Disleide S. Leite Ruben c. O. Andrade Filho Fabiano Ferreira Patricia S. Gouveia Daniel P. Udrisar Maria I. wanderley Department of Physiology and Pharmacology Federal University of Pernambuco 50607-901 Recife PE Brazil

This study investigated the acute effects of green tea extract （GTE） and its polyphenol constituents, （-）-epigallocatechin-3-gallate （EGcG） and （-）-epicatechin （Ec）, on basal and stimulated testosterone production by rat Leydig cells in vitro. Leydig cells purified in a Percoll gradient were incubated for 3 h with GTE, EGcG or Ec and the testosterone precursor androstenedione, in the presence or absence of either protein kinase A （PKA） or protein kinase c （PKc） activators. The reversibility of the effect was studied by pretreating cells for 15 min with GTE or EGcG, allowing them to recover for 1 h and challenging them for 2 h with human chorionic gonadotropin （hcG）, luteinizing hormone releasing hormone （LHRH）, 22（R）-hydroxycholesterol or androstenedione. GTE and EGcG, but not Ec, inhibited both basal and kinase-stimulated testosterone production. Under the pretreatment conditions, the inhibitory effect of the higher concentration of GTE/EGcG on hcG/LHRH-stimulated or 22（R）- hydroxycholesterol-induced testosterone production was maintained, whereas androstenedione-supported testosterone production returned to control levels. At the lower concentration of GTE/EGcG, the inhibitory effect of these polyphenols on 22（R）-hydroxycholesterol-supported testosterone production was reversed. The inhibitory effects of GTE may be explained by the action of its principal component, EGcG, and the presence of a gallate group in its structure seems important for its high efficacy in inhibiting testosterone production. The mechanisms underlying the effects of GTE and EGcG involve the inhibition of the PKA/PKc signalling pathways, as well as the inhibition of P450 side-chain cleavage enzyme and 17β-hydroxysteroid dehydrogenase function.

关键词： green tea polyphenols Leydig cells protein kinase A protein kinase c testosterone

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Genotype-environment interaction in cordia trichotoma(Vell.)Arrá*** *** in two different soil conditions

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Journal of Forestry Research 2022年第1期33卷 309-319页

作者： wanderley dos Santos Bruno Marchetti de Souza Daniele Fernanda Zulian Gilberto Terra Ribeiro Alves João Bosco Vasconcellos Gomes Mário Luiz Teixeira de Moraes Valderês Aparecida de Sousa Ananda Virginia de Aguiar Faculdade de Engenharia de Ilha Solteira Universidade Estadual Paulista“Júlio Mesquita Filho”Ilha Solteira 15385-000Brazil Sucupira Agroflorestas Fazenda SucupiraValença 45400-000Brazil EMBRAPA Florestas Empresa Brasileira de Pesquisa AgropecuáriaColombo 83411-000Brazil

Investment in silvicultural techniques is noticeably lacking,especially in breeding programs for non-conventional wood *** genotype×environment interaction(G×E)is essential to the development of breeding ***,this study aimed to estimate genetic diversity of and the effects of G×E interaction on two progeny tests of cordia trichotoma,including the estimation of genetic gain and genetic diversity after *** the experiment,30 progenies of *** were tested at two sites with differing soil *** at breast height(1.30 m above soil surface,dbh-cm),total height,diameter at 30 cm from the soil,first branch height,and survival were all monitored for four *** deviance,best linear unbiased estimator,and harmonic mean of relative performance of genetic values(MHPRVG)were all calculated to predict breeding values,estimate genetic parameters,and analyze *** quantified traits varied significantly among progenies by soil type,with greatest variation recorded for genetic *** of the progenies led to predictions of genetic gain,ranging from 7.73 to 15.45%,for dbh at four years of *** calculated decrease in genetic diversity after selection showed that this parameter should be monitored in subsequent breeding cycles.G×E was low for all *** best-performing progenies proved most stable and best adapted to the different environmental conditions tested.

关键词： Effective population number Native species Productivity Stability and adaptability Tree improvement

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Trichomonas vaginalis perturbs the junctional complex in epithelial cells

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cell Research 2005年第9期 29-41页

作者： Rodrigo Furtado MADEIRO da cOSTA,wanderley de SOUZA,Marlene BENcHIMOL,John F ALDERETE,José Andrés MORGADO-DíAZ Programa de Pós Graduao em ciências Morfológicas Universidade Federal do Rio de Janeiro Rio de Janeiro RJ 20231- 050 Brazil Grupo de Biologia Estrutural Diviso de Biologia celular centro de Pesquisa Instituto Nacional de cancer Rio de Janeiro RJ 20231-050 Brazil Grupo de Biologia Estrutural Diviso de Biologia celular centro de Pesquisa Instituto Nacional de cancer Rio de Janeiro RJ 20231-050 Brazil Laboratório de Ultraestrutura celular Hertha Meyer Instituto de Biofísica carlos chagas Filho Universidade Federal do Rio de Janeiro centro de ciências da Saúde Rio de Janeiro RJ 21944-900 Brazil Laboratório de Ultraestrutura celular Universidade Santa úrsula Rio de Janeiro RJ 22231-010 Brazil Department of Microbiology University of Texas Health Science center San Antonio TX 78229-3900 USA Grupo de Biologia Estrutural Diviso de Biologia celular centro de Pesquisa Instituto Nacional de cancer Rio de Janeiro RJ 20231-050 Brazil

Trichomonas vaginalis, a protist parasite of the urogenital tract in humans, is the causative agent of trichomonosis, which in recent years have been associated with the cervical cancer development. In the present study we analyzed the modifications at the junctional complex level of caco-2 cells after interaction with two isolates of T. vaginalis and the influence of the iron concentration present in the parasite’s culture medium on the interaction effects. Our results show that T. vaginalis adheres to the epithelial cell causing alterations in the junctional complex, such as: (a) a decrease in transepithelial electrical resistance; (b) alteration in the pattern of junctional complex proteins distribution as observed for E-cadherin, occludin and ZO-1; and (c) enlargement of the spaces between epithelial cells. These effects were dependent on (a) the degree of the parasite virulence isolate, (b) the iron concentration in the culture medium, and (c) the expression of adhesin proteins on the parasite surface.

关键词： Trichomonas vaginalis caco-2 junctional complex proteins transepithelial electrical resistance adhesins.

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clinical criteria for Airway Assessment: correlations with Laryngoscopy and Endotracheal Intubation conditions

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Open Journal of Anesthesiology 2013年第7期3卷 320-325页

作者： Gustavo Henrique S. wanderley Luciana cavalcanti Lima Tania cursino de Menezes couceiro Waston Vieira Silva Raquel Queiroz G. A. coelho Andrea cavalcanti c. Lucena Anne Danielle Santos Soares Agreste Regional Hospital Caruaru Brazil Brazilian Society of Anesthesiology Accredited Teaching and Training center IMIP Recife Brazil. Instituto de Medicina Integral Professor Fernando Figueira (IMIP) Recife Brazil

Difficult intubation, inadequate ventilation and esophageal intubation are the principal causes of death or brain damage related to airway manipulation. The objective of this cross-sectional study was to correlate a preanesthetic evaluation that may be capable of predicting a difficult intubation with the conditions encountered at laryngoscopy and endotracheal intubation. Eighty-one patients submitted to general anesthesia were evaluated at a preanesthetic consultation according to the modified Mallampati classification, the Wilson score and the American Society of Anesthesiologists (ASA) difficult airway algorithm. Findings were then correlated with the cormack-Lehane classification and with the number of attempts at endotracheal intubation. No statistically significant correlations were found between the patients’ Mallampati classification and their cormack-Lehane grade or between the Mallampati classification and the number of attempts required to achieve endotracheal intubation. Laryngoscopy proved difficult in four patients and in all of these cases the Wilson score had been indicative of a possibly difficult airway, highlighting its good predicting sensitivity. However, the specificity of this test was low, since another 24 patients had the same Wilson score but were classified as cormack-Lehane I/II. Moreover, two patients who had a Wilson score ≥ 4 were also classified as cormack-Lehane grade I/II. The study concluded that the Wilson score, although seldom used in clinical practice, is a highly sensitive predictor of a difficult airway;its specificity, however, is low.

关键词： Preanesthetic Evaluation Respiratory System Airway Endotracheal Intubation Measurement Tech-niques Mallampati Wilson cormack-Lehane

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Seroprevalence of hepatitis E in adults in Brazil:a systematic review and metaanalysis

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Infectious Diseases of Poverty 2019年第1期8卷 97-97页

作者： Fatima Mitiko Tengan Gerusa M.Figueiredo Arielle K.S.Nunes carol Manchiero Bianca P.Dantas Mariana c.Magri Thamiris V.G.Prata Marisa Nascimento celso c.Mazza Edson Abdala Antonio A.Barone wanderley M.Bernardo Department of Infeaious and Parasitic Diseases School of MedicineUniversity of Sao Paulo(Universidade de Sao Paulo-USP)Sao PauloSPBrazil Laboratory of Viral Medical Research in Hepatology(Laboratorio de Investigagao Medica em Hepatologia por virus-LIM-47) Clinics HospitalSchool of MedicineUSPSao PauloSPBrazil 不详

Background:Hepatitis E virus(HEV)is a member of the Hepeviridae family;it has four main genotypes and one *** genotypes 1 and 2 cause epidemic hepatitis and are transmitted via water and the fecal-oral route,genotypes 3 and 4 are zoonotic In the various seroprevalence studies of hepatitis E in Brazil,the numbers reported vary widely and are difficult to *** aim of this study was to analyze existing seroprevalence studies of hepatitis E in adults in *** text:We searched the PubMed,Latin American and caribbean Health Sciences and Embase databases for studies published from inception to May 12,2018 concerning infection by HEV in Brazil without time period or language *** included studies that presented data concerning hepatitis E seroprevalence in adults in Brazil,had a sample size>50 patients and whose method used for the detection of anti-HEV was standardized and *** also evaluated the quality of the articles using a list of criteria that totalized 9 *** the 20 studies ultimately analyzed,10(50%)were from the southeast region of Brazil,3(15%)were from the central-west region,3(15%)were from the northern region,2(10%)were from the northeast region and 2(10%)were from the southern *** the quality evaluation of the studies,the mean score was 5.6(range:4-8).The estimated overall seroprevalence of HEV infection in the adult population was 6.0%(95%cI:5.0-7.0);in subgroup analyses,we observed that the prevalence of anti-HEV antibodies in blood donors was 7.0%(95%cI:5.0-8.0),whereas in the general population,it was 3.0%(95%cI:2.0-4.0).conclusions:The results of this systematic review indicate that there should be national investment in the prevention of hepatitis E virus infection in Brazil,including the implementation of improvements in basic sanitation and guidance regarding the appropriate handling of animal waste and the optimal cooking of vegetables,meat and their derivatives.

关键词： Hepatitis E Hepatitis E virus Brazil Prevalence Systematic review

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Bone marrow-derived monocyte infusion improves hepatic fibrosis by decreasing osteopontin,TGF-β1,IL-13 and oxidative stress

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World Journal of Gastroenterology 2017年第28期23卷 5146-5157页

作者： Veruska cintia Alexandrino de Souza Thiago Almeida Pereira Valéria wanderley Teixeira Helotonio carvalho Maria carolina Accioly Brelaz de castro carolline Guimaraes D’assuncao Andreia Ferreira de Barros camila Lima carvalho Virgínia Maria Barros de Lorena Vláudia Maria Assis costa Alvaro Aguiar coelho Teixeira Regina celia Bressan Queiroz Figueiredo Sheilla Andrade de Oliveira centro de Pesquisas Aggeu Magalhaes Fundacao Oswaldo Cruz Immunopathogenesis Section Laboratory of Parasitic DiseasesNational Institute of Allergy and Infectious DiseaseNational Institutes of HealthBethesdaMDUSA and Laboratório de Doencas Parasitárias e infecciosasFaculdade de MedicinaUniversidade Federal de Minas Gerais Departamento de Morfologia e Fisiologia Animal Universidade Federal Rural de Pernambuco Departamento de Biofísica e Radiobiologia Universidade Federal de Pernambuco centro Acadêmico de Vitória Universidade Federal de Pernambuco Departamento de Medicina Tropical Universidade Federal de Pernambuco

To evaluate the therapeutic effects of bone marrow-derived cD11b+cD14+ monocytes in a murine model of chronic liver *** liver damage was induced in c57BL/6 mice by administration of carbon tetrachloride and ethanol for 6 mo. Bone marrow-derived monocytes isolated by immunomagnetic separation were used for therapy. The cell transplantation effects were evaluated by morphometry, biochemical assessment, immunohistochemistry and enzyme-linked immunosorbent ***11b+cD14+ monocyte therapy significantly reduced liver fibrosis and increased hepatic glutathione levels. Levels of pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin (IL)-6 and IL-1β, in addition to pro-fibrotic factors, such as IL-13, transforming growth factor-β1 and tissue inhibitor of metalloproteinase-1 also decreased, while IL-10 and matrix metalloproteinase-9 increased in the monocyte-treated group. cD11b+cD14+ monocyte transplantation caused significant changes in the hepatic expression of α-smooth muscle actin and *** therapy is capable of bringing about improvement of liver fibrosis by reducing oxidative stress and inflammation, as well as increasing anti-fibrogenic factors.

关键词： Monocytes Bone marrow mononuclear cells cell therapy Macrophages Glutathione Liver fibrosis

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