Trichomonas vaginalis perturbs the junctional complex in epithelial cells

作     者：Rodrigo Furtado MADEIRO da COSTA,Wanderley de SOUZA,Marlene BENCHIMOL,John F ALDERETE,José Andrés MORGADO-DíAZ 

作者机构：Programa de Pós Graduao em Ciências Morfológicas Universidade Federal do Rio de Janeiro Rio de Janeiro RJ 20231- 050 Brazil Grupo de Biologia Estrutural Diviso de Biologia Celular Centro de Pesquisa Instituto Nacional de Cancer Rio de Janeiro RJ 20231-050 Brazil Grupo de Biologia Estrutural Diviso de Biologia Celular Centro de Pesquisa Instituto Nacional de Cancer Rio de Janeiro RJ 20231-050 Brazil Laboratório de Ultraestrutura Celular Hertha Meyer Instituto de Biofísica Carlos Chagas Filho Universidade Federal do Rio de Janeiro Centro de Ciências da Saúde Rio de Janeiro RJ 21944-900 Brazil Laboratório de Ultraestrutura Celular Universidade Santa úrsula Rio de Janeiro RJ 22231-010 Brazil Department of Microbiology University of Texas Health Science Center San Antonio TX 78229-3900 USA Grupo de Biologia Estrutural Diviso de Biologia Celular Centro de Pesquisa Instituto Nacional de Cancer Rio de Janeiro RJ 20231-050 Brazil 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：2005年第9期

页      面：29-41页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Trichomonas vaginalis Caco-2 junctional complex proteins transepithelial electrical resistance adhesins. 

摘      要：Trichomonas vaginalis, a protist parasite of the urogenital tract in humans, is the causative agent of trichomonosis, which in recent years have been associated with the cervical cancer development. In the present study we analyzed the modifications at the junctional complex level of Caco-2 cells after interaction with two isolates of T. vaginalis and the influence of the iron concentration present in the parasite’s culture medium on the interaction effects. Our results show that T. vaginalis adheres to the epithelial cell causing alterations in the junctional complex, such as: (a) a decrease in transepithelial electrical resistance; (b) alteration in the pattern of junctional complex proteins distribution as observed for E-cadherin, occludin and ZO-1; and (c) enlargement of the spaces between epithelial cells. These effects were dependent on (a) the degree of the parasite virulence isolate, (b) the iron concentration in the culture medium, and (c) the expression of adhesin proteins on the parasite surface.