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Effects of dietary supplementation of bacteriophage cocktail on health status of weanling pigs in a non-sanitary environment

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Journal of Animal Science and Biotechnology 2023年第4期14卷 1661-1677页

作者： Yohan Choi Abdolreza Hosseindoust Sang Hun ha Joeun kim YeJin Min YongDae Jeong JunYoung Mun SooJin Sa JinSoo kim Swine Science Division National Institute of Animal ScienceRural Development AdministrationCheonan 31000Republic of Korea Department of Animal Industry Convergence Kangwon National UniversityChuncheon 24341Republic of Korea

Background The study evaluated the effects of bacteriophage cocktail(BP)and ZnO administered during weaning time for piglets exposed to a non-sanitary *** bacteriophages were designed to eliminate Escherichia coli(K88,K99 and F41),Salmonella(typhimurium and enteritidis),and Clostridium perfreingens(types A and C).Forty 21-day-old crossbreed piglets were assigned to four treatments,including the PC(sanitary environment),NC(nonsanitary environment),BP(NC plus 108 pfu/kg BP),and ZO(NC plus 2,500 mg/kg ZnO).Piglets in the NC,BP and ZO were kept in a non-sanitary environment for 14 d,which was contaminated with the feces of infected *** Pigs in the BP and ZO treatments had a higher final body weight compared with the *** NC treatment showed the highest concentration of inflammatory cytokines including interleukin(IL)-1β,IL-6 and tumor necrosis factor-αin the *** administration of BP and ZO showed lower myeloperoxidase concentrations compared with the *** NC treatment showed a lower concentration of superoxide dismutase in serum compared with the *** the treatments in non-sanitary environment,the NC treatment showed a higher concentration of malondialdehyde compared with the *** PC treatment showed a lower concentration of butyric acid in the feces compared with the BP *** non-sanitary treatments,the villus height in the duodenum was greater in the BP and ZO compared with the *** lower abundance of Proteobacteria phylum was observed in the BP and PC treatments compared with the *** highest relative abundance of Eubacterium was recorded in the BP *** abundance of Megasphaera and Schwartzia was higher in the NC pigs compared with the BP *** abundance of Desulfovibrio was lower in the supplemented treatments(BP and ZO)compared with non-supplemented(NC and PC).The abundance of Cellulosilyticum genera was higher in the BP and ZO treatments rather than in the *** piglets in the NC treatment had the

关键词： Antioxidant Cytokines Diarrhea Fecal score Inflammation Microbiota Proteobacteria

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Real-time and label-free biosensing using moiré pattern generated by bioresponsive hydrogel

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生物活性材料(英文) 2023年第5期23卷 383-393页

作者： Semin kim Geehong kim Yong Woo Ji Chae-Eun Moon Yuna Jung hyung Keun Lee JaeJong Lee Won-Gun Koh Department of Chemical and Biomolecular Engineering Yonsei University50 Yonsei-roSeodaemun-guSeoul120-749Republic of Korea Korea Institute of Machinery&Materials Nano-Convergence Mechanical Systems Research Division156 Gajeongbuk-roYuseong-guDaejeon34103Republic of Korea Department of Ophthalmology Yongin Severance HospitalYonsei University College of Medicine363Dongbaekjukjeon-daeroGiheung-guYongin-siGyeonggi-do16995Republic of Korea Institute of Vision Research Department of OphthalmologyYonsei University College of Medicine50-1 Yonsei-roSeodaemun-guSeoul120-749Republic of Korea Department of Ophthalmology Graduate School of Medical ScienceBrain Korea 21 ProjectYonsei University College of MedicineRepublic of Korea College of Pharmacy Yonsei University85 Songdo Hwawak-roYeonsu-guIncheon26426Republic of Korea Department of Ophthalmology Gangnam Severance HospitalYonsei University College of Medicine211 Eonju-roGangnam-guSeoul06273Republic of Korea Department of Nanomechatronics University of Science and Technology217Gajeong-roYuseong-guDaejeon 34113Republic of Korea

Bioresponsive hydrogels are smart materials that respond to various external stimuli and exhibit great potential as biosensors owing to their capability of real-time and label-free ***,we propose a sensing platform based on bioresponsive hydrogels,employing the concept of moiré *** sets of line patterns with different pitch sizes are prepared;a hydrogel grating whose pitch size changes according to external stimuli and a reference grating with constant pitch *** volume changes of the hydrogel caused by external stimuli changes the pitch size of the hydrogel grating,and subsequently,the pitch sizes of the moiré patterns(moiré signal),whose values can be obtained in a real-time and label-free manner through customized moiré microscopy and signal *** confirming that the pH-induced swelling of hydrogel could be monitored using moiré patterns,we performed moiré pattern-based detection of specific proteins using protein-responsive hydrogel that underwent shrinking via interaction with target ***-derived neurotrophic factor and platelet-derived growth factor were selected as the model proteins,and our proposed system successfully detected both proteins at nanomolar *** both cases,the pitch size change of hydrogel grating was monitored much more sensitively using moiré patterns than through direct *** changes in the moiré signals caused by target proteins were detected in ex-vivo environments using a custom-made intraocular lens incorporating the hydrogel grating,demonstrating the capability of the proposed system to detect various markers in intraocular aqueous humor,when implanted in the eye.

关键词： Bioresponsive hydrogel Moirépattern Biosensing Intraocular lens Aqueous humor

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CNN Accelerator Using Proposed Diagonal Cyclic Array for Minimizing Memory Accesses

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Computers, Materials & Continua 2023年第8期76卷 1665-1687页

作者： Hyun-Wook Son Ali AAl-hamid Yong-Seok Na Dong-Yeong Lee hyung-Won kim Department of Electronics College of Electrical and Computer EngineeringChungbuk National UniversityCheongju28644Korea Department of Electrical Engineering College of EngineeringAl-Azhar UniversityCairo11651Egypt

This paper presents the architecture of a Convolution Neural Network(CNN)accelerator based on a newprocessing element(PE)array called a diagonal cyclic array(DCA).As demonstrated,it can significantly reduce the burden of repeated memory accesses for feature data and weight parameters of the CNN models,which maximizes the data reuse rate and improve the computation ***,an integrated computation architecture has been implemented for the activation function,max-pooling,and activation function after convolution calculation,reducing the hardware *** evaluate the effectiveness of the proposed architecture,a CNN accelerator has been implemented for You Only Look Once version 2(YOLOv2)-Tiny consisting of 9 ***,the methodology to optimize the local buffer size with little sacrifice of inference speed is presented in this *** implemented the proposed CNN accelerator using a Xilinx Zynq ZCU102 Ultrascale+Field Programmable Gate Array(FPGA)and ISE Design *** FPGA implementation uses 34,336 Look Up Tables(LUTs),576 Digital Signal Processing(DSP)blocks,and an on-chip memory of only 58 KB,and it could achieve accuracies of 57.92% and 56.42% mean Average Precession@0.5 thresholds for intersection over union(mAP@0.5)using quantized 16-bit and 8-bit full integer data manipulation with only 0.68% as a loss for 8-bit version and computation time of 137.9 and 69 ms for each input image respectively using a clock speed of 200 *** speeds are expected to be doubled five times using a clock speed of 1GHz if implemented in a silicon System on Chip(SoC)using a sub-micron process.

关键词： CNN accelerator systolic array memory optimization YOLOv2-tiny mAP@0.5

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Long-term radiofrequency electromagnetic fields exposure attenuates cognitive dysfunction in 5×FAD mice by regulating microglial function

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Neural Regeneration Research 2023年第11期18卷 2497-2503页

作者： Yeonghoon Son Hye-Jin Park Ye Ji Jeong hyung-Do Choi Nam kim hae-June Lee Division of Radiation Biomedical Research Korea Institute of Radiological&Medical SciencesSeoulKorea Department of EMF Research Team Radio and Broadcasting Technology LaboratoryElectronics and Telecommunications Research InstituteDaejonKorea School of Electrical and Computer Engineering Chungbuk National UniversityCheongjuKorea

We have previously found that long-term effects of exposure to radiofrequency electromagnetic fields in 5xFAD mice with severe late-stage Alzheimer’s disease reduced both amyloid-βdeposition and glial activation,including *** examine whether this therapeutic effect is due to the regulation of activated microglia,we analyzed mic roglial gene expression profiles and the existence of microglia in the brain in this study.5xFAD mice at the age of 1.5 months were assigned to sham-and radiofrequency electromagnetic fields-exposed groups and then animals were exposed to 1950 MHz radiofrequency electromagnetic fields at a specific absorption rate of 5 W/kg for 2 hours/day and 5 days/week for 6 *** conducted behavioral tests including the object recognition and Y-maze tests and molecular and histopathological analysis of amyloid precursor protein/a myloid-beta metabolism in brain *** confirmed that radiofrequency electromagnetic field exposure for 6 months ameliorated cognitive impairment and amyloid-β*** expression levels of Iba1(pan-microglial marker)and colony-stimulating factor 1 receptor(CSF1R;regulates microglial prolife ration)in the hippocampus in 5xFAD mice treated with radiofrequency electromagnetic fields were significantly reduced compared with those of the sham-exposed ***,we analyzed the expression levels of genes related to mic rogliosis and microglial function in the radiofrequency electromagnetic fields-exposed group compared to those of a CSF1R inhibitor(PLX3397)-treated *** radiofrequency electromagnetic fields and PLX3397 suppressed the levels of genes related to microgliosis(Csf1r,CD68,and Ccl6)and pro-inflammatory cytokine interleukin-1β.N otably,the expression levels of genes related to mic roglial function,including Trem2,Fcgr1α,Ctss,and Spi1,were decreased after long-term radiofrequency electromagnetic field exposure,which was also observed in response to microglial suppression by *** res

关键词： 5×FAD Alzheimer’s disease CSF1R long term exposure microglial function neuroinflammation radiofrequency electromagnetic fields therapeutic effect

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Recent advances in soft electronic materials for intrinsically stretchable optoelectronic systems

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Opto-Electronic Advances 2022年第8期5卷 21-49页

作者： Ja Hoon Koo Huiwon Yun Woongchan Lee Sung-Hyuk Sunwoo hyung Joon Shim Dae-Hyeong kim Center for Nanoparticle Research Institute for Basic Science(IBS)Seoul 08826Republic of Korea School of Chemical and Biological Engineering Institute of Chemical ProcessesSeoul National UniversitySeoul 08826Republic of Korea Department of Materials Science and Engineering Seoul National UniversitySeoul 08826Republic of Korea.

In recent years,significant progress has been achieved in the design and fabrication of stretchable optoelectronic *** general,stretchability has been achieved through geometrical modifications of device components,such as with serpentine interconnects or buckled ***,the local stiffness of individual pixels and the limited pixel density of the array have impeded further advancements in stretchable ***,intrinsically stretch-able optoelectronics have been proposed as an alternative ***,we review the recent advances in soft elec-tronic materials for application in intrinsically stretchable optoelectronic ***,we introduce various intrinsically stretchable electronic materials,comprised of electronic fillers,elastomers,and surfactants,and exemplify different in-trinsically stretchable conducting and semiconducting *** also describe the processing methods used to fabricate the electrodes,interconnections,charge transport layers,and optically active layers used in intrinsically stretch-able optoelectronic ***,we review representative examples of intrinsically stretchable optoelectronic devices,including light-emitting capacitors,light-emitting diodes,photodetectors,and ***,we briefly discuss intrinsically stretchable integrated optoelectronic systems.

关键词： stretchable optoelectronics light-emitting capacitors light-emitting diodes photodetectors photovoltaics intrinsically stretchable devices

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Role of Surgery on Growth of Tricuspid Valve in Pulmonary Atresia with Intact Ventricular Septum:Mid-Term Results of Modified Right-Ventricular Overhauling Procedure

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Congenital Heart Disease 2023年第3期18卷 325-336页

作者： Jae Gun Kwak Eung Re kim Taeyoung Yun Sungkyu Cho Chang-ha Lee Woong-han kim Department of Thoracic and Cardiovascular Surgery Seoul National University Children’s HospitalCollege of MedicineSeoul National UniversitySeoulKorea 2Department of Thoracic and Cardiovasclar SurgerySejong General HospitalBucheonGyeonggiKorea

Objectives:To access the effectiveness of our modified right-ventricular overhauling procedure on tricuspid valve(TV)growth in patients with pulmonary atresia with intact ventricular septum(PAIVS).Methods:We retrospectively reviewed 21 patients with PAIVS who underwent modified right ventricular overhauling(mRVoh)between 2008 and 2019 at two *** mRVoh consisted of wide resection of hypertrophied infundibular and trabecular muscle,peeling off fibrotic endocardial tissue in the right ventricle(RV)cavity,surgical pulmonary valvotomy,and Blalock-Taussig shunt or banding of ductus arteriosus under cardiopulmonary *** TV annulus sizes were measured and analyzed using echocardiography before and after ***:No mortalities were observed during a median follow-up of 3 years(interquartile range:1.3–4.7 years)of follow-up were *** was performed at a median age of 163.5 days(range:21–560 days),including seven neonates and two infants(hanged during the period after mRVoh(−2.03 to−1.61,p=0.028)compared with the period before mRVoh(−2.51→–2.03,p=0.575)after percutaneous intervention ***:mRVoh in PAIVS patients was positively associated with TV annular growth,and it was more effective than percutaneous RVOT widening interventions without mRVoh.

关键词： Congenital heart disease cyanotic heart disease pulmonary atresia with intact ventricular septum right ventricular overhauling

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High-performance solid-solution potassium-ion intercalation mechanism of multilayered turbostratic graphene nanosheets

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Journal of Energy Chemistry 2022年第4期31卷 814-823页

作者： Jiae Um Seung Uk Yoon Hoseong kim Beom Sik Youn Hyoung-Joon Jin hyung-Kyu Lim Young Soo Yun Program in Environmental and Polymer Engineering Inha Universityncheon 22212 South Korea Department of Polymer Science and Engineering Inha UniversityIncheon 22212South Korea Division of Chemical Engineering and Bioengineering Kangwon National University Chuncheon Gangwon-do 24341 South Korea KU-KIST Graduate School of Converging Science and Technologyea University 145 Anam-roSeongbuk-guSoul02841 South Korea Department of Integrative Energy Engineering 145 Anam-roSongbuk-guKorea UniversitySeoul 02841 South Korea

The solid-solution reaction between an alkali cation and an active host material is known as a singlephase redox mechanism,and it is typically accompanied by a continuous voltage *** is distinct from the typical alkali cation intercalation reaction at an equivalent site of the active host material,which exhibits a voltage ***,we report an unusual solid-solution potassium-ion intercalation mechanism with a low-voltage plateau capacity on multilayered turbostratic graphene nanosheets(T-GNSs).Despite the disordered graphitic structure with a broad range of d-spacings(3.65–4.18À),the T-GNSs showed a reversible plateau capacity of~200 m A h g^(-1),which is higher than that of a well-ordered graphite nanoplate(~120 m A h g^(-1)).In addition,a sloping capacity of~220 m A h g^(-1)was delivered with the plateau capacity,and higher rate capabilities,better reversibility,and a more stable cycling performance were confirmed on the turbostratic ***-principles calculations suggest that the multitudinous lattice domains of the T-GNSs contain diverse intercalation sites with strong binding energies,which could be the origin of the high-performance solid-solution potassium-ion intercalation behavior when the turbostratic graphene stacks have a d-spacing smaller than that of equilibrium potassium–graphite intercalation compounds(5.35À).

关键词： Turbostratic graphite Graphene nanosheet Defective carbon Anode Potassium-ion batteries

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Supramolecular Polymer Intertwined Free-Standing Bifunctional Membrane Catalysts for All-Temperature Flexible Zn-Air Batteries

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Nano-Micro Letters 2022年第11期14卷 341-360页

作者： Nayantara K.Wagh Sambhaji S.Shinde Chi Ho Lee Sung-hae kim Dong-hyung kim han-Don Um Sang Uck Lee Jung-Ho Lee Department of Materials Science and Chemical Engineering Hanyang UniversityAnsanRepublic of Korea Department of Applied Chemistry Center for Bionano Intelligence Education and ResearchHanyang UniversityAnsanRepublic of Korea Department of Chemical Engineering Kangwon National UniversityChuncheonGangwon 24341Republic of Korea

Rational construction of flexible free-standing electrocatalysts featuring long-lasting durability,high efficiency,and wide temperature tolerance under harsh practical operations are fundamentally significant for commercial zinc-air ***,3D flexible free-standing bifunctional membrane electrocatalysts composed of covalently cross-linked supramolecular polymer networks with nitrogen-deficient carbon nitride nanotubes are fabricated(referred to as PEMAC@NDCN)by a facile self-templated ***@NDCN demonstrates the lowest reversible oxygen bifunctional activity of 0.61 V with exceptional long-lasting durability,which outperforms those of commercial Pt/C and RuO_(2).Theoretical calculations and control experi-ments reveal the boosted electron transfer,electrolyte mass/ion transports,and abundant active surface site ***,the constructed alkaline Zn-air battery with PEMAC@NDCN air-cathode reveals superb power density,capacity,and discharge-charge cycling stability(over 2160 cycles)compared to the reference Pt/C+RuO_(2).Solid-state Zn-air batteries enable a high power density of 211 mW cm^(−2),energy density of 1056 Wh kg^(−1),stable charge-discharge cycling of 2580 cycles for 50 mA cm^(−2),and wide temperature tolerance from−40 to 70℃with retention of 86%capacity compared to room-temperature counterparts,illustrating prospects over harsh operations.

关键词： Flexible free-standing membrane electrocatalysts Supramolecular polymer Alkaline and flexible solid-state Zn-air batteries All-temperature operations High capacity and energy density

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Flexible kesterite thin-film solar cells under stress

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npj Flexible Electronics 2022年第1期6卷 863-870页

作者： ha Kyung Park Yunae Cho Juran kim Sammi kim Sungjun kim Jeha kim Kee-Jeong Yang Dae-Hwan kim Jin-Kyu Kang William Jo Department of Physics Ewha Womans UniversitySeoul 03760Republic of Korea Division of Energy Technology Daegu Gyeongbuk Institute of Science and Technology(DGIST)Daegu 42988Republic of Korea Department of Solar&Energy Engineering Cheongju UniversityCheongju 28503Republic of Korea

Understanding the stress-induced phenomena is essential for improving the long-term application of flexible solar cells to non-flat ***,we investigated the electronic band structure and carrier transport mechanism of Cu2ZnSn(S,Se)4(CZTSSe)photovoltaic devices under mechanical *** efficient flexible CZTSSe devices were fabricated controlling the Na *** electronic structure of CZTSSe was deformed with stress as the band gap,valence band edge,and work function *** properties of the bent CZTSSe surface were probed by Kelvin probe force microscopy and the CZTSSe with Na showed less degraded carrier transport compared to the CZTSSe without *** local open-circuit voltage(VOC)on the bent CZTSSe surface decreased due to limited carrier *** reduction of local VOC occurred larger with convex bending than in concave bending,which is consistent with the degradation of device *** study paves the way for understanding the stress-induced optoelectronic changes in flexible photovoltaic devices.

关键词： optoelectronic stress bending

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A Remark on Strong Law of Large Numbers for Weighted U-Statistics

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Acta Mathematica Sinica,English Series 2014年第9期30卷 1595-1605页

作者： hyung-Tae ha Mei Ling HUANG Department of Applied Statistics Gachon University Department of Mathematics and Statistics Brock University

Let （X, Xn; n≥ 1} be a sequence of i.i.d, random variables with values in a measurable space （S,8） such that E｜h（X1, X2,..., Xm）｜〈 ∞, where h is a measurable symmetric function from Sm into R = （-∞, ∞）. Let {wn,i1,i2 im ; 1 ≤ i1 〈 i2 〈 …… 〈im 〈 n, n ≥ m} be a matrix array of real numbers. Motivated by a result of Choi and Sung （1987）, in this note we are concerned with establishing a strong law of large numbers for weighted U-statistics with kernel h of degree m. We show that whenever SUP n≥m max1〈i1〈i2〈…〈im≤｜wn i1,i2 i,im｜〈∞, where 0 = Eh（X1, X2,..., Xm）. The proof of this result is based on a new general result on complete convergence, which is a fundamental tool, for array of real-valued random variables under some mild conditions.

关键词： Strong law of large numbers weighted U-statistics complete convergence

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