Real-time and label-free biosensing using moiré pattern generated by bioresponsive hydrogel

作     者：Semin Kim Geehong Kim Yong Woo Ji Chae-Eun Moon Yuna Jung Hyung Keun Lee JaeJong Lee Won-Gun Koh 

作者机构：Department of Chemical and Biomolecular EngineeringYonsei University50 Yonsei-roSeodaemun-guSeoul120-749Republic of Korea Korea Institute of Machinery&MaterialsNano-Convergence Mechanical Systems Research Division156 Gajeongbuk-roYuseong-guDaejeon34103Republic of Korea Department of OphthalmologyYongin Severance HospitalYonsei University College of Medicine363Dongbaekjukjeon-daeroGiheung-guYongin-siGyeonggi-do16995Republic of Korea Institute of Vision ResearchDepartment of OphthalmologyYonsei University College of Medicine50-1 Yonsei-roSeodaemun-guSeoul120-749Republic of Korea Department of OphthalmologyGraduate School of Medical ScienceBrain Korea 21 ProjectYonsei University College of MedicineRepublic of Korea College of PharmacyYonsei University85 Songdo Hwawak-roYeonsu-guIncheon26426Republic of Korea Department of OphthalmologyGangnam Severance HospitalYonsei University College of Medicine211 Eonju-roGangnam-guSeoul06273Republic of Korea Department of NanomechatronicsUniversity of Science and Technology217Gajeong-roYuseong-guDaejeon 34113Republic of Korea 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2023年第23卷第5期

页      面：383-393页

核心收录：

学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 

基　　金：This work was supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(Ministry of Science and ICT)(NRF-2017M3A7B4041798 NRF-2021R1A2C4001596 and NRF-2017M3D1A1039289) 

主　　题：Bioresponsive hydrogel Moirépattern Biosensing Intraocular lens Aqueous humor 

摘      要：Bioresponsive hydrogels are smart materials that respond to various external stimuli and exhibit great potential as biosensors owing to their capability of real-time and label-free ***,we propose a sensing platform based on bioresponsive hydrogels,employing the concept of moiré *** sets of line patterns with different pitch sizes are prepared;a hydrogel grating whose pitch size changes according to external stimuli and a reference grating with constant pitch *** volume changes of the hydrogel caused by external stimuli changes the pitch size of the hydrogel grating,and subsequently,the pitch sizes of the moiré patterns(moiré signal),whose values can be obtained in a real-time and label-free manner through customized moiré microscopy and signal *** confirming that the pH-induced swelling of hydrogel could be monitored using moiré patterns,we performed moiré pattern-based detection of specific proteins using protein-responsive hydrogel that underwent shrinking via interaction with target ***-derived neurotrophic factor and platelet-derived growth factor were selected as the model proteins,and our proposed system successfully detected both proteins at nanomolar *** both cases,the pitch size change of hydrogel grating was monitored much more sensitively using moiré patterns than through direct *** changes in the moiré signals caused by target proteins were detected in ex-vivo environments using a custom-made intraocular lens incorporating the hydrogel grating,demonstrating the capability of the proposed system to detect various markers in intraocular aqueous humor,when implanted in the eye.