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绿茶外泌体样纳米颗粒(ELNs)延缓人体成纤维细胞衰老作用研究

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茶叶通讯 2024年第3期51卷 391-400页

作者：程敏宋景鋈雷轩灏李皓楠陈思贝赵进管峰葛建中国计量大学生命科学学院浙江杭州310018

探究绿茶外泌体样纳米颗粒(ELNs)及其复方配伍对人体表皮成纤维细胞衰老模型的干预作用。通过超速离心法结合蔗糖密度梯度离心,提取纯化绿茶ELNs,采用透射电镜、粒径检测以及脂质组学分析对ELNs进行体外表征。CCK-8法证明24 h内ELNs对... 详细信息

探究绿茶外泌体样纳米颗粒(ELNs)及其复方配伍对人体表皮成纤维细胞衰老模型的干预作用。通过超速离心法结合蔗糖密度梯度离心,提取纯化绿茶ELNs,采用透射电镜、粒径检测以及脂质组学分析对ELNs进行体外表征。CCK-8法证明24 h内ELNs对人体表皮成纤维细胞具有明显诱导增殖功能;生化分析和分子检测显示,ELNs能显著提高人体表皮成纤维细胞超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶(GSH-px)活力,降低丙二醛(MDA)含量,并提高羟脯氨酸(HYP)、Ⅲ型胶原蛋白(COL3)含量,同时抑制基质金属蛋白酶-1(MMP-1)、P53和P16基因的表达。与番茄红素复配,具有显著协同抗衰老作用。结果表明绿茶ELNs及其复方能显著延缓人成纤维细胞衰老,主要干预细胞生化代谢过程和相关基因表达。

关键词：绿茶外泌体样纳米颗粒(ELNs) 天然活性分子成纤维细胞衰老

基于层次分析模型的潜艇极低频电磁场贡献率评估方法研究

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中国测试 2024年第8期50卷 41-50页

作者：付鸿宇潘孟春张琦胡佳飞管峰徐昱静黄博李海滨陈棣湘刘中艳国防科技大学智能科学学院湖南长沙410073 海军研究院北京100161

潜艇极低频电磁场作为目标探测的关键特征,其形成机制复杂多样,平台工况以及在水下所处位置都会对其产生很大影响。有效操控以降低潜艇的磁特征对于提升其磁隐身性能具有重要的意义。然而,当前各种因素对电磁场影响程度的研究尚不充分... 详细信息

潜艇极低频电磁场作为目标探测的关键特征,其形成机制复杂多样,平台工况以及在水下所处位置都会对其产生很大影响。有效操控以降低潜艇的磁特征对于提升其磁隐身性能具有重要的意义。然而,当前各种因素对电磁场影响程度的研究尚不充分。为此该文提出一种基于层次分析模型的贡献率评估方法,旨在为有效操控提供理论依据。首先深入分析潜艇极低频电磁场的威胁成因,并建立相应的威胁层次结构模型。其次,通过仿真手段获取由不同成因产生的电磁场信号特征,并构建威胁矩阵和特征矩阵。最后,计算得到不同成因电磁场对总电磁场的贡献率,在尾部测线上,尾流磁场的贡献率占比最高,达到0.7649;径向测线上轴频磁场贡献率占比最高,并逐渐衰减,最终在150 m处低于尾流电磁场,并保持在占比0.5左右。仿真结果验证了该方法的有效性,可为潜艇极低频电磁场的综合主动控制策略研究奠定技术基础。

关键词：极低频电磁场贡献率评估层次分析模型(AHP) 特征提取

Methyl-CpG binding domain(MBD)2/3 specifically recognizes and binds to the genomic mCpG site with a β-sheet in the MBD to affect embryonic development in Bombyx mori

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Insect Science 2023年第6期30卷 1607-1621页

作者： Tong-Yu Fu Shuang-Shun Ji Yu-Lin Tian Yi-guang Lin Yu-Mei Chen Qi-En Zhong Si-Chun Zheng guan-feng Xu guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Guangzhou Key Laboratory of Insect Development Regulation and Application ResearchInstitute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina

Methyl-CpG(mCpG)binding domain(MBD)proteins especially bind with methylated DNA,and are involved in many important biological processes;however,the binding mechanism between insect MBD2/3 and mCpG remains *** this study,we identified 2 isoforms of the MBD2/3 gene in Bombyx mori,MBD2/3-S and MBD2/*** analysis of MBD2/3-L,MBD2/3-S,and 7 mutant MBD2/3-L proteins deficient inβ1−β6 orα1 in the MBD showed thatβ2−β3-turns in theβ-sheet of the MBD are necessary for the formation of the MBD2/3–mCpG complex;furthermore,other secondary structures,namely,β4−β6 and anα-helix,play a role in stabilizing theβ-sheet structure to ensure that the MBD is able to bind *** addition,sequence alignment and binding analyses of different insect MBD2/3s indicated that insect MBD2/3s have an intact and conserved MBD that binds to the mCpG of target ***,MBD2/3 RNA interference results showed that MBD2/3-L plays a role in regulating *** embryonic development,similar to that of DNA methylation;however,MBD2/3-S withoutβ4−β6 andα-helix does not alter embryonic *** results suggest that MBD2/3-L recognizes and binds to mCpG through the intactβ-sheet structure in its MBD,thus ensuring silkworm embryonic development.

关键词： embryonic development insects MBD2/3 methyl-CpG binding domain(MBD) β-sheet

Effect of flapping‑meridians combined with thunder‑fire moxibustion on upper limb dysfunction after stroke

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Journal of Integrative Nursing 2020年第3期2卷 123-128页

作者： feng‑guang guan Qiu‑Lu JI Yue‑Qing JIANG Zhuo‑Mei ZHUANG Xiao‑Qin LIAN Department of Nursing The Second People’s Hospital Affiliated to Fujian University of Traditional Chinese MedicineFuzhouChina School of Nursing Fujian University of Traditional Chinese MedicineFuzhouChina

Objective:The objective of the study is to explore the effect of flapping‑meridians combined with thunder‑fire moxibustion in the treatment of upper limb dysfunction after *** and Methods:This randomized,single‑blind,controlled trial included 140 patients with *** to the random number table,the patients were divided into four groups:the control group(receiving conventional nursing alone),the intervention group 1(receiving conventional nursing combined with flapping‑meridians),the intervention group 2(receiving conventional nursing combined with thunder‑fire moxibustion),and the intervention group 3(receiving conventional nursing,flapping‑meridians,and thunder‑fire moxibustion).All four groups were intervened 4 weeks,6 days a *** outcome indicators(Fugl‑Meyer Assessment of the upper extremity and body mass index of shoulder abduction,elbow extension,and wrist flexion)were assessed at the beginning and the end of ***:The effect of the intervention group 3 was better than that of the intervention group 2,intervention group 1,and control group(P<0.05).Conclusion:The flapping‑meridians combined with thunder‑fire moxibustion can significantly improve the upper limb motor function and muscle strength of stroke patients with hemiplegia,and its effect is better than that of single treatment.

关键词： Flapping‑meridians hemiplegia stroke thunder‑fire moxibustion

Utility of 64-MSCT in assessing acute non-reperfused myocardial infarct size

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Journal of Geriatric Cardiology 2013年第3期10卷 247-252页

作者： Xin-Kai Qu Wei-Yi Fang Jian-Ding Ye Shao-feng guan Ruo-Gu Li Ying-Jia Xu Yan Shen Min Zhang Hua Liu Department of Cardiology Shanghai Chest Hospital Shanghai Jiaotong University Shanghai 200030 China Department of Radiology Shanghai Chest Hospital Shanghai Jiaotong University Shanghai 200030 China

Objective To evaluate the utility of multi-slice computed tomography (MSCT) in assessing acute non-reperfused myocardial infarct size. Methods Seven domestic pigs (mean weight 17.3 ± 1.9 kg) underwent ligation of the distal left anterior descending artery to establish a model of acute myocardial infarction (MI). MSCT and triphenyltetrazolium chloride (TTC) staining were performed two hours later. The following data were acquired and analyzed:MI volume (%), CT values of the infarcted region, left ventricular cavity and normal cardiac tissue at various scanning time-points (1, 5, 10, 15, 20 min after contrast injection). Results Using MSCT, the overall MI volume showed a time-dependent decrease, with a reduction of 28.87%after 20 min. The greatest reduction occurred at the 5 min time-point. In TTC staining, MI volume was 9.87%± 2.44%. When MI size, as determined by MSCT, was compared with that by TTC staining in Bland-Altman plots, there was a better agreement at 5, 10, and 15 min time-points at 1 and 20 min. Conclusions The study indicates that double-phase scanning examination using MSCT is a useful tool to assess MI size, and the optimal late-phase scanning time-point set within 5-15 min of contrast injection.

关键词： Acute non-reperfused myocardial infarction Animal model Multi-slice computed tomography Time-factors

Three-dimensional porous In_(2)O_(3) arrays for self-powered transparent solar-blind photodetectors with high responsivity and excellent spectral selectivity

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Nano Research 2024年第5期17卷 4471-4477页

作者： Nana Zhang Xinyu Gao Haoran guan Simin Sun Jiaming Liu Zhitao Shao Qiyue Gao Yuan Zhang Ruyu Sun guang Yang feng Gao Wei feng College of Chemistry Chemical Engineering and Resource UtilizationNortheast Forestry UniversityHarbin 150040China Key Laboratory for Photonic and Electronic Bandgap Materials Ministry of EducationSchool of Physics and Electronic EngineeringHarbin Normal UniversityHarbin 150025China

Transparent solar-blind ultraviolet photodetectors(SBUV PDs)have extensive applications in versatile scenarios,such as optical ***,it is still challenging to simultaneously achieve high responsivity,high transparency,and satisfying self-powered ***,we demonstrated high-performance,transparent,and self-powered photoelectrochemical-type(PEC)SBUV PDs based on vertically grown ultrathin In_(2)O_(3) nanosheet arrays(NAs)with a three-dimensional(3D)porous *** 3D porous structure simultaneously improves the transmittance in the visible light region,accelerates interfacial reaction kinetics,and promotes photogenerated carrier *** performance of In_(2)O_(3) NAs photoanodes exceeds most reported self-powered PEC SBUV PDs,exhibiting a high transmittance of approximately 80%in the visible light region,a high responsivity of 86.15 mA/W for 254 nm light irradiation,a fast response speed of 15/18 ms,and good multicycle *** In_(2)O_(3) NAs also show excellent spectral selectivity with an ultrahigh solar-blind rejection ratio of 1319.30,attributed to the quantum confinement effect induced by the ultrathin feature(2-3 nm).Furthermore,In_(2)O_(3) NAs photoanodes show good capability in underwater optical *** work demonstrated that a 3D porous structure is a powerful strategy to synchronously achieve high responsivity and transparency and provides a new perspective for designing high-performance,transparent,and self-powered PEC SBUV PDs.

关键词： In_(2)O_(3) three-dimensional porous transparent photoelectrochemical solar-blind photodetectors

苦瓜枯萎病抗病与感病品种根际土壤的微生物数量消长动态分析

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中国农学通报 2024年第22期40卷 118-124页

作者：石博谢媛媛关峰杨雪桐张景云王凯万新建江西省农业科学院蔬菜花卉研究所/江西省蔬菜分子育种工程研究中心/园艺作物(果蔬茶)育种江西省重点实验室南昌330200

以28个苦瓜品种为材料,通过苗期人工接种枯萎病鉴定其抗性强弱,结果显示,28个苦瓜品种枯萎病发病程度出现较大差异,共获得高抗材料1份、感病材料11份。苦瓜根际微生物生物数量消长动态差异分析结果表明,土壤中尖孢镰刀菌数量与苦瓜抗、... 详细信息

以28个苦瓜品种为材料,通过苗期人工接种枯萎病鉴定其抗性强弱,结果显示,28个苦瓜品种枯萎病发病程度出现较大差异,共获得高抗材料1份、感病材料11份。苦瓜根际微生物生物数量消长动态差异分析结果表明,土壤中尖孢镰刀菌数量与苦瓜抗、感品种枯萎病发生的相关性存在差异,枯萎病的发生与土壤中尖孢镰刀菌数量呈极显著正相关。抗病品种土壤微生物数量结构优于感病品种。抗病苦瓜品种根际微生物群落的丰富度和多样性更高,其真菌的Sobs、Shannon和Chao1指数分别高出36.93%、25.89%和38.58%,细菌的Sobs、Shannon和Chao1指数分别高出13.63%、12.91%和26.52%。感病苦瓜品种土壤中的pH、铵态氮、硝态氮、速效磷和有机质含量在幼苗期均高于抗病苦瓜品种,根际微生物组成受pH、氮素、有效磷等因素的综合影响。

关键词：苦瓜枯萎病抗病性根际微生物土壤理化性质生物多样性

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基于等效阻抗渐变变压器的宽带压控振荡器

微电子学 2024年第3期54卷 382-387页

作者：王晶晶伍兰鑫李振东刘长江开撒尔江·艾买尔陈亚新关峰饶毅恒王浩湖北大学武汉430062

设计了一种基于65 nm CMOS工艺的毫米波宽带压控振荡器(VCO)。分析了应用于宽带VCO的宽带化技术。针对高频相位噪声性能恶化这一问题,提出并设计了一种等效阻抗渐变变压器,以优化VCO高频段相位噪声。该压控振荡器输出信号频率为20.4~40.... 详细信息

设计了一种基于65 nm CMOS工艺的毫米波宽带压控振荡器(VCO)。分析了应用于宽带VCO的宽带化技术。针对高频相位噪声性能恶化这一问题,提出并设计了一种等效阻抗渐变变压器,以优化VCO高频段相位噪声。该压控振荡器输出信号频率为20.4~40.2 GHz,覆盖24~26 GHz、38~39 GHz两个5G毫米波通信频段。全频带内,相位噪声小于-102 dBc/Hz,品质因子大于180.9 dBc/Hz。

关键词：宽带压控振荡器多模式谐振等效阻抗渐变变压器

广东某地食用农产品快速检测实验室监督评价与分析

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实验室检测

实验室检测 2024年第9期2卷 122-126页

作者：苏焕斌冯观萍黄钟标符嘉诚梁祖培广东产品质量监督检验研究院阳江技师学院

目的监督评价食用农产品快速检测实验室，检验当地快速检测工作落实情况。方法在广东省市场监督管理局相关评价细则的基础上，结合政府快速检测实验室运营管理相关要求，从实验室场地、快速检测制度建立、完成情况、抽样情况、原始记... 详细信息

目的监督评价食用农产品快速检测实验室，检验当地快速检测工作落实情况。方法在广东省市场监督管理局相关评价细则的基础上，结合政府快速检测实验室运营管理相关要求，从实验室场地、快速检测制度建立、完成情况、抽样情况、原始记录、后续处理、快速检测操作、快速检测产品及设备管理等环节对快速检测实验室进行全面评价。结果本次对12家食用农产品政府快速检测实验室进行监督评价，综合得分均在89分以上。每个政府快速检测实验室考核6个盲样，只有E政府快速检测实验室盲样考核准确率未达到100%，监督评价结果较为理想。结论当地食用农产品快速检测实验室在发挥快速检测筛查的“防火墙”方面取得一定的成效，但仍存在一定的问题及风险点，建议从人员培训、原始记录、快速检测产品与设备管理、快速检测质量控制、监督考核等方面入手，推动快速检测实验室检测能力不断提升。

关键词：食品安全食用农产品快速检测实验室监督评价