Methyl-CpG binding domain(MBD)2/3 specifically recognizes and binds to the genomic mCpG site with a β-sheet in the MBD to affect embryonic development in Bombyx mori
作者机构：Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied TechnologyGuangzhou Key Laboratory of Insect Development Regulation and Application ResearchInstitute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2023年第30卷第6期
页 面：1607-1621页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 09[农学]
基 金：funded by the National Natural Sci-ence Foundation of China grant numbers 32100374 and 31872286
主 题：embryonic development insects MBD2/3 methyl-CpG binding domain(MBD) β-sheet
摘 要：Methyl-CpG(mCpG)binding domain(MBD)proteins especially bind with methylated DNA,and are involved in many important biological processes;however,the binding mechanism between insect MBD2/3 and mCpG remains *** this study,we identified 2 isoforms of the MBD2/3 gene in Bombyx mori,MBD2/3-S and MBD2/*** analysis of MBD2/3-L,MBD2/3-S,and 7 mutant MBD2/3-L proteins deficient inβ1−β6 orα1 in the MBD showed thatβ2−β3-turns in theβ-sheet of the MBD are necessary for the formation of the MBD2/3–mCpG complex;furthermore,other secondary structures,namely,β4−β6 and anα-helix,play a role in stabilizing theβ-sheet structure to ensure that the MBD is able to bind *** addition,sequence alignment and binding analyses of different insect MBD2/3s indicated that insect MBD2/3s have an intact and conserved MBD that binds to the mCpG of target ***,MBD2/3 RNA interference results showed that MBD2/3-L plays a role in regulating *** embryonic development,similar to that of DNA methylation;however,MBD2/3-S withoutβ4−β6 andα-helix does not alter embryonic *** results suggest that MBD2/3-L recognizes and binds to mCpG through the intactβ-sheet structure in its MBD,thus ensuring silkworm embryonic development.
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