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Molecular characteristics and temperature tolerance function of the transient receptor potential in the native Bemisia tabaci AsiaII3 cryptic species

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Journal of Integrative Agriculture 2020年第11期19卷 2746-2757页

作者： JI Shun-xia SHEN Xiao-na LIANG Lin wanG Xiao-di LIU wan-xue wan fang-hao LV Zhichuang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural SciencesShenzhen 518120P.R.China

Insects are poikilothermic animals,and temperature is one of the most important abiotic factors affecting their spread and *** example,differences in thermal tolerance may underlie the significant differences in geographical distributions between the native AsiaII3 and invasive MED(Mediterranean)cryptic Bemisia tabaci species in *** receptor potential(TRP)channels are key components of the insect temperature perception system and act as molecular thermometers since they can be activated by specific changes in *** this study,we cloned and characterized the AsiaII3 BtTRP gene and revealed its functions in the response to thermal *** full-length cDNA of BtTRP was 3821 bp,with a 3501-bp open reading frame encoding a 132.05-kDa *** the deduced amino acid sequences of AsiaII3 BtTRP and MED TRP revealed five amino acid *** situ hybridization indicated that BtTRP might be widely expressed throughout the AsiaII3 adult *** mRNA expression reached the highest levels after exposure to mild thermal stimuli(12 and 35°C),showing that BtTRP expression can be induced by temperature ***,the thermal tolerance of AsiaII3 after BtTRP dsRNA feeding was significantly lower than that of the *** together,the present study highlights the importance of TRP channels for *** thermal resistance,and allows us to infer that the differences in amino acids between AsiaII3 and MED might cause the differences in thermal tolerance of these two cryptic *** study provides a new direction for investigating geographic distribution differences between invasive and native insects.

关键词： transient receptor potential thermosensation thermal stress Bemisia tabaci AsiaII3

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湖北地区豚草卷蛾发生规律与抗寒性研究

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生物安全学报 2011年第4期20卷 314-316页

作者：褚世海万鹏丛盛波万方浩许冬黄民松武怀恒湖北省农业科学院植保土肥研究所农作物重大病虫草害防控湖北省重点实验室湖北武汉430064 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室北京100193

【背景】豚草卷蛾是豚草的一种重要天敌,1993年在湖南临湘野外释放后,迅速扩散至湖北、江西、福建等省,对豚草种群的蔓延与扩张起到了一定的抑制作用。【方法】为明确豚草卷蛾在湖北的发生规律与抗寒性,2009年在湖北各豚草发生区进行野... 详细信息

【背景】豚草卷蛾是豚草的一种重要天敌,1993年在湖南临湘野外释放后,迅速扩散至湖北、江西、福建等省,对豚草种群的蔓延与扩张起到了一定的抑制作用。【方法】为明确豚草卷蛾在湖北的发生规律与抗寒性,2009年在湖北各豚草发生区进行野外调查,并将豚草卷蛾越冬态老熟幼虫置于不同低温下观测其存活率。【结果】豚草卷蛾主要分布于鄂东地区。其越冬代成虫于5月下旬至6月上旬发生,第1代幼虫发生高峰在6月下旬至7月上旬,10月中下旬老熟幼虫进入滞育。12月份的调查结果显示,豚草有虫株率为90%,虫口密度为313头.百株-1,存活率为93.3%。越冬老熟幼虫过冷却点为-26.4℃,存活率和存活时间随温度的降低而下降。【结论与意义】豚草卷蛾已广泛分布于湖北地区,且能成功越冬,对豚草可起到持续控制作用。

关键词：豚草卷蛾分布发生规律过冷却点抗寒性

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Composite Quantile Estimation for Kink Model with Longitudinal Data

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Acta Mathematica Sinica,English Series 2023年第3期39卷 412-438页

作者： Chuang wan Wei ZHONG Ying fang School of Statistics and Data Science Nankai UniversityTianjin 300071P.R.China MOE Key Lab of Econometrics WISEDepartment of Statistics and Data ScienceSchool of EconomicsXiamen UniversityXiamen 361000P.R.China

Kink model is developed to analyze the data where the regression function is two-stage piecewise linear with respect to the threshold covariate but continuous at an unknown kink *** quantile regression for longitudinal data,kink point where the kink effect happens is often assumed to be heterogeneous across different ***,the kink point tends to be the same across different quantiles,especially in a region of neighboring quantile *** such homogeneity information could increase the estimation efficiency of the common kink *** this paper,we propose a composite quantile estimation approach for the common kink point by combining information from multiple neighboring *** normality of the proposed estimator is *** addition,we also develop a sup-likelihood-ratio test to check the existence of the kink effect at a given quantile level.A test-inversion confidence interval for the common kink point is also developed based on the quantile rank score *** simulation studies show that the proposed composite kink estimator is more efficient than the single quantile regression *** also illustrate the proposed method via an application to a longitudinal data set on blood pressure and body mass index.

关键词： Asymptotical normality composite quantile estimation estimation efficiency kink design model longitudinal data

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Halloween genes AhCYP307A2 and AhCYP314A1 modulate last instar larva–pupa–adult transition, ovarian development and oogenesis in Agasicles hygrophila (Coleoptera:Chrysomelidae)

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Journal of Integrative Agriculture 2023年第3期22卷 812-824页

作者： JIN Ji-su LIU Yi-ran ZHOU Zhong-shi wan fang-hao GUO Jian-ying State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural SciencesShenzhen 518120P.R.China

In insects,ecdysteroids are synthesized by genes of the Halloween family and play important roles in several key developmental events,including molting and ***,the roles of these genes in Agasicles hygrophila are still largely *** this study,the expression patterns of the two Halloween genes AhCYP307A2 and AhCYP314A1 were determined by quantitative PCR(qPCR)at different developmental ***,the functions of these two genes were explored using RNA interference(RNAi),and ovarian development was observed by dissecting the ovaries of *** *** qPCR results showed that AhCYP307A2 and AhCYP314A1 were highly expressed in last instar larvae and in adult *** addition,AhCYP307A2 was also highly expressed in eggs and pupae but was markedly lower than in third-instar larvae and *** RNAi results showed that the injection of dsAhCYP307A2 or dsAhCYP314A1 markedly inhibited their expression and the transcription levels of three related *** of AhCYP307A2 or AhCYP314A1 significantly inhibited larval molting,impaired last instar larva–pupa–adult transition,delayed ovarian development,and stopped egg production(i.e.,no eggs were laid).These results indicate that AhCYP307A2 and AhCYP314A1 play important regulatory roles in last instar larva–pupa–adult transition and reproduction in ***.

关键词： Agasicles hygrophila ovary oogenesis AhCYP307A2 AhCYP314A1 RNA interference

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SUPERCOOLING POINT AND WATER CONTENT IN ADULTS OF DIFFERENT GEOGRAPHICAL OPHRAELLA COMMUNA POPULATIONS

SUPERCOOLING POINT AND WATER CONTENT IN ADULTS OF DIFFERENT ...

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International Congress on Biological Invasions

作者： Min LI~(1,2),Zhong-Shi ZHOU~1,Jian-Ying GUO~1,fang-hao wan~1,and Yuan-Hua LUO~3 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100094,China, 2 College of Plant Protection,Fujian Agricultural University,Fuzhou 350002 China 3 Institute of Plant Protection,Hunan Academy of Agricultural Sciences,Changsha 410125, China

Ophraella communa is originated in North America and feeds on Ambrosia *** of cold hardiness of *** in different geographical environments,the supercooling point(SCP) and water content(WC) in adults of four geographical *** populations(***,Wuchang,Mluo and Nanchang populations) were determined in the *** results showed that the highest temperature of supercooling point was observed in Fuzhou population,with the value of -11.67℃,and the lowest was noticed in Wuchang population,which was -15.1467℃.No difference was observed in SCPs between Nanchang population and Miluo *** content in Fuzhou population reached a maximum among four different populations,of 73.41%.However,SCP and WC were not significantly affected by *** on the results of this experiment,cold-hardiness of O. communa might be significantly affected by geographical environments.

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我国西花蓟马线粒体DNA-COⅠ基因变异及群体遗传结构分析

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生物安全学报 2014年第3期23卷 196-209页

作者：张桂芬乔玮娜古君伶闵亮万方浩中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室北京100193 农业部外来入侵生物预防与控制研究中心北京100193 北京市顺义区植保植检站北京101300

【背景】西花蓟马于2003年入侵中国后,迅速传播扩散并在局部区域造成严重危害,对我国蔬菜、花卉及果树产业的健康发展构成了巨大威胁。【方法】以线粒体DNA标记技术,对采自我国13个省(市)不同地理区域西花蓟马种群的175条COⅠ基因进行... 详细信息

【背景】西花蓟马于2003年入侵中国后,迅速传播扩散并在局部区域造成严重危害,对我国蔬菜、花卉及果树产业的健康发展构成了巨大威胁。【方法】以线粒体DNA标记技术,对采自我国13个省(市)不同地理区域西花蓟马种群的175条COⅠ基因进行序列变异及群体遗传结构分析。【结果】试验共检测到13个单倍型,各地理种群西花蓟马的单倍型多样性Hd较高,为0.691,而核苷酸多样性π较低,为0.00652。总体固定指数Fst为0.24359,基因流Nm为0.78;种群之间固定指数和基因交流分析表明,我国各地理种群间可能出现了一定分化。种群间AMOVA分析显示,我国西花蓟马的遗传变异主要来自种群内部。对国内外不同地理种群COⅠ基因序列的单倍型进行聚类分析表明,我国西花蓟马有2个品系,即温室品系和羽扇豆品系,其中羽扇豆品系来源于新西兰和荷兰,而广泛存在的温室品系存在多个入侵来源。【结论与意义】我国西花蓟马各地理种群的发生,既与国际贸易活动有关,又与国内蔬菜、花卉、水果等的调运以及观光旅游密不可分。研究结果对西花蓟马的有效阻截及其种群扩张趋势监测意义重大。

关键词：西花蓟马线粒体DNA 基因变异遗传结构地理种群单倍型

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平衡强化训练对住院老年精神分裂症患者平衡能力的影响:一项随机对照试验

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中国全科医学 2025年第2期28卷 193-199页

作者：秦伟涂刚英万小梅王芳江西省精神卫生中心护理部江西省南昌市330029 南昌大学附属精神病医院护理部江西省南昌市330029 江西省精神卫生中心老年科江西省南昌市330029

背景住院老年精神疾病患者跌倒发生率呈上升趋势,而运动不足和久坐不动的生活方式成为住院老年患者的现状,平衡训练可以有效减少跌倒已被证实,但在精神科运用甚少。目的探讨平衡强化训练对住院老年精神分裂症患者平衡能力的影响。方法选... 详细信息

背景住院老年精神疾病患者跌倒发生率呈上升趋势,而运动不足和久坐不动的生活方式成为住院老年患者的现状,平衡训练可以有效减少跌倒已被证实,但在精神科运用甚少。目的探讨平衡强化训练对住院老年精神分裂症患者平衡能力的影响。方法选取2023年江西省某三级甲等精神专科医院老年科住院精神分裂症患者72例作为研究对象,采用随机数字表法分为试验组(36例)和对照组(36例)。对照组采用常规康复训练,试验组在对照组基础上采用平衡强化训练方案进行干预,在干预前及干预4、12周后,使用Berg平衡量表(BBS)和中文版Barthel指数(BI)以及国际版跌倒效能量表(FES-I)对两组患者进行评估。结果共62例患者完成训练,其中试验组30例,对照组32例。两因素重复测量方差分析显示,组别与时间对BBS、BI评分存在交互作用(P0.05),时间对BBS、BI评分主效应均显著(P0.05);时间对FES-I总分及室内、室外活动评分主效应均显著(P<0.05)。干预12周,试验组FES-I总分及室内、室外活动评分高于对照组(P<0.05)。组内比较显示,试验组干预4周FES-I总分及室内、室外活动评分均高于干预前(P<0.05);试验组干预12周FES-I总分及室内、室外活动评分均高于干预前、干预4周(P<0.05)。结论平衡强化训练方案可有效提高住院老年精神分裂症患者平衡能力、生活自理能力及跌倒自我效能,降低跌倒风险。

关键词：精神分裂症老年人平衡强化训练平衡能力跌倒随机对照试验

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Range expansion of the invasive cotton mealybug, Phenacoccus solenopsis Tinsley: An increasing threat to agricultural and horticultural crops in China

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Journal of Integrative Agriculture 2020年第3期19卷 881-885页

作者： wanG Yu-sheng DAI Tian-mei TIAN Hu wan fang-hao ZHANG Gui-fen State Key Laboratory for Biology of Plant Diseases and Insect Pests/Key Laboratory of Integrated Pest Management of Crop Ministry of Agriculture and Rural Affairs/Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests College of ForestryCentral South University of Forestry and TechnologyChangsha 410004P.R.China Caofeidian Customs House Tangshan 063210P.R.China Center for Management of Invasive Alien Species Ministry of Agriculture and Rural AffairsBeijing 100193P.R.China

The globally invasive cotton mealybug Phenacoccus solenopsis Tinsley,has recently invaded several Chinese regions,and continues to expand its *** mealybug is a polyphagous insect pest,with at least 200 host plants from 63 families,including cotton,food crops,fruits,ornamental plants,tobacco,and ***,we describe the first finding of *** in Shandong Province,and investigate the abundances of its natural enemies(predator Coccinella septempunctata *** parasitoid Aenasius bambawalei Hayat).All three species were detected from ornamental and weed species in Linyi,Shandong,in September 2018,and were positively identified using DNA *** analysis revealed significant identity(99.38-100%) between sequences of *** obtained in this study and those published in the *** invasive pest represents a major potential threat to agri-horticulture in Shandong,which is a key province of China for production and export of many vegetables,flowers,fruits,and other *** mealybug species has a wide range of potential distribution in Shandong and is easily dispersed with agricultural and horticultural transport *** monitoring and management strategies are required to limit the spread of this pest species.

关键词： Aenasius bambawalei Coccinella septempunctata field investigation invasive insect pest molecular identification Phenacoccus solenopsis rapid spreading

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Regulation and integration of plant jasmonate signaling:a comparative view of monocot and dicot

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Journal of Genetics and Genomics 2022年第8期49卷 704-714页

作者： Shiwei wan Xiu-fang Xin National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China University of Chinese Academy of Sciences Beijing 100049China CAS-JIC Center of Excellence for Plant and Microbial Sciences(CEPAMS) Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China

The phytohormone jasmonate plays a pivotal role in various aspects of plant life,including developmental programs and defense against pests and pathogens.A large body of knowledge on jasmonate biosynthesis,signal transduction as well as its functions in diverse plant processes has been gained in the past two *** addition,there exists extensive crosstalk between jasmonate pathway and other phytohormone pathways,such as salicylic acid(SA)and gibberellin(GA),in co-regulation of plant immune status,fine-tuning the balance of plant growth and defense,and so on,which were mostly learned from studies in the dicotyledonous model plants Arabidopsis thaliana and tomato but much less in ***,existing evidence suggests both conservation and functional divergence in terms of core components of jasmonate pathway,its biological functions and signal integration with other phytohormones,between monocot and *** this review,we summarize the current understanding on JA signal initiation,perception and regulation,and highlight the distinctive characteristics in different lineages of plants.

关键词： Jasmonate Phytohormone Signaling Crosstalk Plant defense Development Stress response

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A REVIEW ON RESEARCH ON DEVELOPMENT OF LIGAMENT CONSTITUTIVE RELATIONS ON MACRO, MESO, AND MICRO LEVELS

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Acta Mechanica Solida Sinica 2013年第4期26卷 331-343页

作者： Chao wan Zhixiu hao Shizhu Wen State Key Laboratory of Tribology Tsinghua University

Ligaments are densely connective soft tissues capable of maintaining stability and function of knee *** an important factor,the constitutive relation of ligament would affect its biomechanics and further play an essential role in the research on ligament injury,healing and *** objective of this paper is to provide an overview of the current research on ligament constitutive relations on the macro,meso,and micro levels as well as the anatomy and histological structure of *** studies of biomechanical behaviors during ligament injury and healing periods have also been *** on the research on ligament constitutive relation in the past three decades,a discussion of some research perspectives is also presented,such as a validated accurate measuring method of in situ strain in ligament,a new constitutive relation involving the distribution of ultra-structural properties,and a rational estimation of ligament injury and healing process by the change of its ultra-structural or histological characteristics.

关键词： constitutive relation ligament ultra-structure macro meso micro

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