Molecular characteristics and temperature tolerance function of the transient receptor potential in the native Bemisia tabaci AsiaII3 cryptic species

作     者：JI Shun-xia SHEN Xiao-na LIANG Lin WANG Xiao-di LIU Wan-xue WAN Fang-hao LV Zhichuang JI Shun-xia;SHEN Xiao-na;LIANG Lin;WANG Xiao-di;LIU Wan-xue;WAN Fang-hao;Lü Zhi-chuang

作者机构：State Key Laboratory for Biology of Plant Diseases and Insect PestsInstitute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Agricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2020年第19卷第11期

页      面：2746-2757页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090402[农学-农业昆虫与害虫防治] 

基　　金：This work was funded by the National Natural Science Foundation of China(31672088 and 31601695) the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(caascx-2017-2022-IAS) the National Key Research and Development Program of China(2016YFC1200603) 

主　　题：transient receptor potential thermosensation thermal stress Bemisia tabaci AsiaII3 

摘      要：Insects are poikilothermic animals,and temperature is one of the most important abiotic factors affecting their spread and *** example,differences in thermal tolerance may underlie the significant differences in geographical distributions between the native AsiaII3 and invasive MED(Mediterranean)cryptic Bemisia tabaci species in *** receptor potential(TRP)channels are key components of the insect temperature perception system and act as molecular thermometers since they can be activated by specific changes in *** this study,we cloned and characterized the AsiaII3 BtTRP gene and revealed its functions in the response to thermal *** full-length cDNA of BtTRP was 3821 bp,with a 3501-bp open reading frame encoding a 132.05-kDa *** the deduced amino acid sequences of AsiaII3 BtTRP and MED TRP revealed five amino acid *** situ hybridization indicated that BtTRP might be widely expressed throughout the AsiaII3 adult *** mRNA expression reached the highest levels after exposure to mild thermal stimuli(12 and 35°C),showing that BtTRP expression can be induced by temperature ***,the thermal tolerance of AsiaII3 after BtTRP dsRNA feeding was significantly lower than that of the *** together,the present study highlights the importance of TRP channels for *** thermal resistance,and allows us to infer that the differences in amino acids between AsiaII3 and MED might cause the differences in thermal tolerance of these two cryptic *** study provides a new direction for investigating geographic distribution differences between invasive and native insects.