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A cost-ef.ective, simple and high-throughput method f.r DNA extraction f.om insects

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Insect Science 2010年第5期17卷 465-470页

作者： Malgorzata Lagisz gordon Port Kirsten Wolf. School of.Biology Newcastle University Newcastle upon Tyne UK

We present a high-throughput cost-ef.ective method to extract DNA suitable f.r polymerase chain reaction （PCR） f.om insect tissue. The method uses standard 200μL- deep 96-well plates in which samples are ground, digested and subsequently purif.ed. The test extraction using f.ur dif.erent insect species and controlling f.r potential contamination showed that the method yields good-quantity and quality DNA. PCR with mitochondrial and nuclear primers was reliable. The proposed extraction protocol combines the speed of.commercial 96-well plate methods with the economies associated with readily available and cheap laboratory chemicals, consumables and equipment. Theref.re, this method is particularly suitable f.r low-budget research projects and f.r laboratories with only basic equipment present.

关键词： DNA extraction genetic isolation molecular technique

f.uorescence Enhancement Ratio Dropdown at Low f.uences during f.mtosecond Double Pulse Laser Ablation

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Chinese Physics Letters 2008年第7期25卷 2653-2656页

作者： Sima SINGHA Robert J. gordon 胡湛 Institute of.Atomic and Molecular Physics Jilin University Changchun 130021 Department of.Chemistry University of Illinois at Chicago Chicago Illinois 60607-7061 USA

In the study of.double pulse ablation of.materials （silicon and copper）, a dropdown of.double pulse to single pulse f.uorescence signal enhancement at low f.uences is observed. The dropdown is analysed with a simple theoretical one-dimensional heat dif.usion model and verif.ed by f.uorescence time constants change as a f.nction of.f.uence. The dropdown is explained as a result of.liquid-solid mixture layer at the liquid and solid boundary. The ef.ect of.the layer becomes important at low f.uences.

关键词： the power-law exponents precipitation durative abrupt precipitation change

Transient thermal analysis as measurement method f.r IC package structural integrity

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Chinese Physics B 2015年第6期24卷 47-64页

作者： Alexander Han Maximilian Schmid E Liu gordon Elger Technische Hochschule Ingolstadt

Practices of.IC package reliability testing are reviewed brief.y, and the application of.transient thermal analysis is examined in great depth. f.r the design of.light sources based on light emitting diode （LED） ef.icient and accurate reliability testing is required to realize the potential lif.times of.105 h. Transient thermal analysis is a standard method to determine the transient thermal impedance of.semiconductor devices, e.g. power electronics and LEDs. The temperature of.the semiconductor junctions is assessed by time-resolved measurement of.their f.rward voltage （Vf.. The thermal path in the IC package is resolved by the transient technique in the time domain. This enables analyzing the structural integrity of.the semiconductor package. However, to evaluate thermal resistance, one must also measure the dissipated energy of.the device （i.e., the thermal load） and the k-f.ctor. This is time consuming, and measurement errors reduce the accuracy. To overcome these limitations, an innovative approach, the relative thermal resistance method, was developed to reduce the measurement ef.ort, increase accuracy and enable automatic data evaluation. This new way of.evaluating data simplif.es the thermal transient analysis by eliminating measurement of.the k-f.ctor and thermal load, i.e. measurement of.the lumen f.ux f.r LEDs, by normalizing the transient Vf.data. This is especially advantageous f.r reliability testing where changes in the thermal path, like cracks and delaminations, can be determined without measuring the k-f.ctor and thermal load. Dif.erent f.ilure modes can be separated in the time domain. The sensitivity of.the method is demonstrated by its application to high- power white InGaN LEDs. f.r detailed analysis and identif.cation of.the f.ilure mode of.the LED packages, the transient signals are simulated by time-resolved f.nite element （f.） simulations. Using the new approach, the transient thermal analysis is enhanced to a powerf.l tool f.r reliability

关键词： transient thermal analysis thermal resistance reliability light emitting diode

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Status of.semen analysis comment on “A survey on the status of.semen analysis in 118 laboratories in China” by Jin-Chun Lu et al. in Asian Journal of.Andrology

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Asian Journal of.Andrology 2010年第1期12卷 115-117页

作者： Hugh William gordon Baker Department of.Obstetrics and Gynaecology University of Melbourne and Melbourne IVF Reproductive Services TheRoyal Women＇s Hospital Parkville 3052 Victoria Australia

The paper by Lu et al. [1] ＂A survey on the status of.semen analysis in 118 laboratories in China＂ provides a major review of.the current methods of.semen analysis in China's Mainland. It involved a 36-item questionnaire completed by technicians in 118 of.a possible 145 potential semen laboratories. Recruitment f.r the study was via contacts developed at Chinese Andrology meetings and training sessions between 2005 and 2007. It is not stated how comprehensive this strategy would be f.r accessing all semen laboratories in China. About half.the participating laboratories were in general hospitals and the others in hospitals with academic af.iliations or f.mily planning institutes.

关键词： no english key word

Why so little ef.ort to study anti-oxidant therapy in burns?

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Burns & Trauma 2016年第3期4卷 167-168页

作者： gordon L.Klein Department of.Orthopaedic Surgery and Rehabilitation University of Texas Medical Branch and Shriners Burns Hospital301 University BoulevardGalvestonTX 77555-0165USA

Given that oxidative stress is an inherent response to burn injury,it is puzzling as to why investigation into anti-oxidant therapy as an adjunct to burn treatment has been *** the inf.ammatory response and the stress response to burn injury involve oxidative stress,and there has been some limited success in studies using gamma tocopherol and selenium to improve certain consequences of.*** remains to be done to investigate the number,doses and combinations of.anti-oxidants,their ef.icacy,and limitations in improving def.ned outcomes af.er burn injury.

关键词： Anti-oxidant Burn Oxidative stress

Commentary f.r Sequential Therapy Based on Evolvement of.Patterns: A New Model f.r Treatment of.Alzheimer's Disease

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Chinese Journal of.Integrative Medicine 2019年第8期25卷 563-564页

作者： gordon Wilcock David Neary Department of.Clinical Neuroscience University of.Oxf.rd Manchester Centre f.r Clinical Neurosciences Salf.rd Royal NHS f.undation Trust

It has been accepted f.r many years that Alzheimer’s disease(AD)is the main cause of.dementia in most if.not all parts of.the *** is a very complex disorder and its underlying pathological mechanisms are still poorly... 详细信息

关键词： Alzheimer A New Model f.r Treatment of.Alzheimer’s Disease Commentary f.r Sequential Therapy Based on Evolvement of.Patterns Model

Dancing to a somewhat dif.erent rhythm: Cell migration along thenatural basement membrane

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BIOCELL 2022年第9期46卷 2059-2063页

作者： SHELDON R.gordon Department of.Biological Sciences Oakland UniversityRochesterMI48309-4476USA

Much of.our understanding of.the events which underlie cell migration has been derived f.om studies of.cells intissue culture. One of.the components that mediates this process is the dynamic actin-based microf.lament system that canreorganize itself.into so-called stress f.bers that are considered essential components f.r cell motility. In contrast, relativelyf.w studies have investigated cell movement along an extracellular matrix (ECM) which is known to inf.uence both cellularorganization and behavior. This opinion/viewpoint article brief.y reviews cell migration during corneal endothelial woundrepair along the tissue’s natural basement membrane, Descemet’s membrane. Because the tissue exists as a cell monolayer itaf.ords one an opportunity to readily explore the ef.ect of.cell/matrix inf.uences on cell motility. As such, cell movementalong this substrate dif.ers somewhat f.om that f.und in vitro and migrating endothelial cells also demonstrate an abilityto move along the ECM without the benef.t of.having an organized actin cytoskeleton.

关键词： Corneal endothelium Cell migration Microf.laments Basement membrane

Oral product input to the GI tract： GIS an oral product perf.rmance technology

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f.ontiers of.Chemical Science and Engineering 2017年第4期11卷 516-520页

作者： gordon L. Amidon Yasuhiro Tsume College of.Pharmacy University of Michigan Ann Arbor MI 48109 USA

The patient receives a pharmaceutical product, not a drug. The pharmaceutical products are f.rmulated with a drug, an active ingredient to produce the maximum therapeutic ef.ect af.er oral absorption. Theref.re, it is the product we must optimize f.r the patients. In order to assure the saf.ty and ef.icacy of.pharmaceutical products, we need an in vivo predictive tool f.r oral product perf.rmance in patients. Currently, we are a surprisingly long way f.om accomplishing that objective. If.the 20th century was the ＇age of.the drug＇, i.e., the ＇magic bullet＇, the 21st century must become the ＇age of.the guided missile＇, i.e., the delivery system, including the f.rm of.the active pharmaceutical ingredient （API）（＇drug＇）. The physical f.rm of.the drug and the delivery system must be optimized to maximize the therapeutic benef.ts of.pharmaceutical products f.r humans. Oral immediate release （IR） dosage f.rms cannot be optimal f.r all drugs or likely even any drugs （APIs）. Still, the f.rmulation of.pharmaceutical products has to be optimized f.r patients. But how do we optimize oral delivery of.drugs？ It is usually through ＇trial and error＇, in humans! We need a better way to optimize the oral dosage f.rms. We have suggested to select dif.erent dissolution methodologies f.r this optimization based on BCS Subclasses. In this article, we present the predicted in vivo drug dissolution prof.le of.ketoconazole as a model drug f.om our laboratory utilizing a gastrointestinal simulator （GIS）, which is an adaptation of.the ASD system. GIS consists of.three chambers representing stomach, duodenum, and jejunum, to create the human gastrointestinal tract-like environment and enable the control the gastric emptying rate. This dissolution system allows the monitoring of.the drug dissolution phenomena and the observation of.the super- saturation and the precipitation of.pharmaceutical pro- ducts, which is usef.l inf.rmation to predict in vivo dissolution of.pharmaceutical products. This

关键词： GIS in vivo predictive dissolution ketocona-zole BCS subclassif.cation supersaturation

How the “f.lding f.nnel” Depends on Size and Structure of.Proteins?A View f.om the Scoring f.nction Perspective

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Tsinghua Science and Technology 2013年第5期18卷 462-468页

作者： Sheng-You Huang gordon K. Springer the Research Support Computing University of Missouri Bioinformatics Consortium and Department of Computer Science University of Missouri

It has been well accepted that the f.lding energy landscape may resemble a f.nnel according to the theory of.protein f.lding. This theory of.＂f.lding f.nnel＂ has been extensively studied and thought to play an important role in guiding the sampling process of.the protein f.lding and ref.nement in protein structure prediction. Here, we have investigated the relationship between the ＂f.nnel likeness＂ of.protein f.lding and the size/structure of.the proteins based on a set of.non-homologous proteins we have recently evaluated using a statistical mechanicsbased scoring f.nction ITScorePro. It was f.und that larger proteins that consist of.more helix/sheet structures tend to have a higher score-Root Mean Square Deviation（RMSD） correlation（or a more f.nnel like energy landscape）.Another measurement in protein f.lding, Z-score, has also shown some correlation with the size of.the *** expected, proteins with a better ＂olding f.nnel likeness＂（or score-RMSD correlation） tend to have a betterpredicted conf.rmation with a lower RMSD f.om their native structures. These f.ndings can be extremely valuable f.r the development and improvement of.sampling and scoring algorithms f.r protein structure prediction.

关键词： energy landscape f.lding f.nnel protein structure prediction scoring f.nction protein f.lding

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