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Prevalence of Human Leukocyte Antigen HLA-B*5701 in HIV-1 Infected Individuals in Brazil

Open Journal of Genetics 2014年第1期4卷 56-62页

作者： Claudinéia de Araújo Cristina Valetta de Carvalho Miriam estela de Souza Freire Amanda Yamaguti Ivens Cuiabano Scaff Fernando José de Souza Flávia Galindo Silvestre Silva Ricardo Sobhie Diaz Ismael Dale Cotrim Guerreiro da Silva Department of Gynecology Molecular Gynecology Laboratory Federal University of S&atildeo Paulo S&atildeo Paulo Brazil State Department of Health of Mato Grosso Cuiabá Brazil Unirondon University Cuiabá Brazil Infectious Diseases Division Federal University of Mato Grosso Cuiabá Brazil Imunogenetics Laboratory General University Hospital Cuiabá Brazil

This study was designed to establish the prevalence of HLA-B*5701 at HIV-1 infected individuals in Brazil. A total of 517 consecutive individuals were followed in this study from February 2009 through July 2011. The presence of HLA-B*5701 was determined by Nested-PCR with HLA-B*57 and HLA-B*5701 sequence-specific primers (PCR-SSP). The expression of HLA-B*57 was negative in the 385 (74.5%) and positive in the 103 (19.9%) of infected individuals. Among these, the expression of HLA-B5701 was positive in the 29 (5.6%) of individuals. No demographic or ethnic differences were found between HLA-B*57/HLA-B*5701 HIV-1 negative patients, with a prevalence of Caucasians (57.1%) individuals. During the period of study, 68 patients were submited to an abacavir contain- ing regimen. The HLA-B*5701 allele was observed in 7 (10.3%) patients, with a significant incidence of Hypersensitivity reactions at 4 of them (p < 0.001). Conclusions: Although Brazilian population consists of a mixture of individuals of Caucasian, African and Native American genetic background, prevalence of HLA-B*5701 in this population is similar to the one found in pure Caucasians.

关键词： Immunogenetics Human Immunodeficiency Virus HLA-B*5701 Antigen Human Abacavir

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Detection of β-Glucuronidase Activity within Actinomadura madurae Grains of Human Actinomycetoma

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Advances in Microbiology 2014年第6期4卷 317-323页

作者： Alejandro Palma-Ramos Samantha Reyes-Mayén Laura estela Castrillón-Rivera Silvia Elena Fernández-López Carmen Padilla-Desgarennes María Elisa Vega-Memije Roberto Arenas-Guzmán María Elisa Drago-Serrano Teresita Sainz-Espunes Department of Biological Systems Universidad Autónoma Metropolitana-XochimilcoMexico CityMéxico Dermatology Center“Dr.Ladislao de la Pascua” Mycology ServiceMexico City Health SecretaryMexico CityMéxico General Hospital“Manuel Gea González” Dermatology ServiceMexico City Health SecretaryMexico CityMéxico General Hospital“Manuel Gea González” Mycology ServiceMexico City Health SecretaryMexico CityMéxico

Actinomycetoma syndrome by Actinomadura (A.) madurae is characterized by a subcutaneous chronic lesion that affects fascia, muscle and bone. A. madurae produces colonies that form grains of less than 1 mm in diameter. Grains are surrounded and infiltrated by neutrophils involved in the grain disruption by enzymes like β-glucuronidase released after the neutrophil degranulation. The aim of this work was to evaluate the polysaccharide degradation of grains treated with β-glucuronidase and to detect the presence and activity of β-glucuronidase within the A. madurae grains. Actinomadura madura grains from patients infected were processed to quantify the total content of polysaccharide with the phenol-sulfuric acid reaction. Grains were treated with β-glucuronidase at different conditions to evaluate the optimal polysaccharide degradation. Grains were analyzed to detect the enzyme by using anti-human β-glucuronidase antibody while enzymatic activity was assessed by evaluating the release of reduced sugars and by in situ enzymatic activity. Optimal degradation of polysaccharide in the grains treated with β-glucuronidase was found with 300 units/ml of enzyme and 24 hr of incubation at 37°C. Presence and activity of β-glucuronidase enzyme within the grains were detected. Results suggested that β-glucuronidase present within A. madurae grain resulted from degranulated neutrophils surrounding and/or infiltrated within the grain.

关键词： Actinomycetoma A. madurae β-Glucuronidase

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回味精品-巨石牛智利高端葡萄酒

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葡萄酒 2020年第7期

作者： estela

1961年,阿楚拉家族在智利Curicó山谷亲手种下第一株葡萄藤时,未曾想过在短短50多年的时间内,这里出产的葡萄酒得到全球众多消费者的青睐。如今,由阿楚拉家族创建的南方山谷酒业集团是智利第8大葡萄酒生产商,行销全球30多个国家,每一天...

1961年,阿楚拉家族在智利Curicó山谷亲手种下第一株葡萄藤时,未曾想过在短短50多年的时间内,这里出产的葡萄酒得到全球众多消费者的青睐。如今,由阿楚拉家族创建的南方山谷酒业集团是智利第8大葡萄酒生产商,行销全球30多个国家,每一天全球各地都会饮用掉32800瓶葡萄酒。南方山谷酒业集团旗下的巨石牛系列(Toro de Piedra)是酒庄的经典葡萄酒之一,巨石牛品牌故事十分有趣:南方山谷创始人Santiago Achurra Larraín购买了一头壮硕有力的公牛,在田野漫步数天之后,这头强壮的公牛竟突然消失在空气中,取而代之的是出现（共1页)

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在酒店的转角，当雪茄遇上威士忌

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葡萄酒 2020年第9期

作者： estela

1969年,曾风靡一时的好莱坞影片《长征万宝山》里有这样一句话:"也许你并不认同,但请你相信我:除非你已经抽了一口上好的雪茄并且喝了一口威士忌,否则你将错过生活中第二件和第三件美好的事情。"雪茄与威士忌的搭配,很早就已流行开来。...

1969年,曾风靡一时的好莱坞影片《长征万宝山》里有这样一句话:"也许你并不认同,但请你相信我:除非你已经抽了一口上好的雪茄并且喝了一口威士忌,否则你将错过生活中第二件和第三件美好的事情。"雪茄与威士忌的搭配,很早就已流行开来。在五星级酒店的转角,当雪茄遇上威士忌,又会碰撞出什么样的故事?（共4页)

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Speakeasy，去冰室里喝杯酒！

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葡萄酒 2020年第5期

作者： estela

广州的老话说得好:东山的少爷,西关的小姐。要想寻找老广州的痕迹,东山口和荔湾可是不能错过的两个老城区。东山口的庙前西街,看似是一条寻常的老街道,但实际上却大有千秋。顺着这条路走下去,藏匿于暗门背后的庙前冰室里正在上演一场精...

广州的老话说得好:东山的少爷,西关的小姐。要想寻找老广州的痕迹,东山口和荔湾可是不能错过的两个老城区。东山口的庙前西街,看似是一条寻常的老街道,但实际上却大有千秋。顺着这条路走下去,藏匿于暗门背后的庙前冰室里正在上演一场精彩的爵士乐表演,神奇的发酵正在沫茗研究所的玻璃罐里悄悄发生,Charlie's Café穿着白色衬衫的调酒师正拿起雪克壶准备调制一杯花好月圆。你,准备好了吗?（共6页)

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Culture Circle as a Teaching Approach in the Education of Teenager Health Multipliers on Leprosy Awareness

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Health 2015年第14期7卷 1813-1823页

作者： estela Maria Leite Meirelles Monteiro Amanda Araújo das Merces Amanda Carla Borba de Souza Cavalcanti Ana Márcia Tenorio de Souza Cavalcanti Ana Catarina Torres de Lacerda Rosália Daniela Medeiros da Silva Andrea Rosane Sousa Silva Waldemar Brandao Neto Graduate Program in Nursing Federal University of Pernambuco (UFPE) Recife Brazil Graduate Program in Child and Adolescent Health Centre of Health Sciences Federal University of Pernambuco Recife Brazil Nursing Department at the Federal University of Pernambuco Recife Brazil

Objective: The study aims to evaluate the use of a teaching method proposed by Paulo Freire, Culture Circles, in the education of teenagers multipliers on leprosy awareness. Methods: It is an action-research study with a qualitative approach developed in a public school in Pernambuco, Brazil. Five Culture Circles were conducted involving the participation of 26 teenagers. The followings were used as data collection tools: observation, field notes, photography and filming. Results: The educational intervention on health addressed the following topics: 1) Definition and transmission of leprosy;2) Characteristics and diagnosis of leprosy;3) Treatment of leprosy;4) Aesthetics, prejudice and mental health related to leprosy;and 5) Planning of educational activities for teenagers as health multipliers on leprosy awareness. The educational action on health provided this age group to perceive themselves, act as political subjects in the development of Culture Circles, and act as protagonists in the dissemination of knowledge on leprosy. Conclusions: This study highlights that the application of active methodologies, such as Culture Circles, is able to encourage the engagement of young people in community empowerment and bring together health professionals and the school community in an intersectoral work in order to develop action strategies involving the promotion of health in the context of neglected diseases such as leprosy.

关键词： Adolescents School Health Leprosy Awareness Culture Circles Health Promotion

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