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Gas storage in shale pore system:A review of the mechanism,control and assessment

Petroleum Science 2023年第5期20卷 2605-2636页

作者： Yue Feng xian-Ming Xiao en-Ze Wang Ping Gao Chen-Gang Lu Gang Li School of energy Resources China University of Geosciences(Beijing)Beijing100083China School of Earth and Space Sciences Peking UniversityBeijing100871China

In the past 15 years,the shale gas revolution and large-scale commercial developments in the United States have driven the exploration and development of shale plays *** many factors affecting shale gas exploration potential,the gas-bearing properties of shale(quantity,storage state,composition)and their controlling factors are the essential research attracting wide attention in the academic *** paper reviews the research progress on the retention mechanism,influencing factors,and evaluation methods for resource potential of the shale gas system,and proposes further research *** is the main mechanism of gas retention in organic-rich shales;the gas is mainly stored in nanopores of shale in free and sorption *** presence of water and nonhydrocarbon gases in pores can complicate the process and mechanism of methane(CH4)sorption,and the related theoretical models still need further *** in-situ gas content and gasbearing properties of shale are governed by the geological properties(organic matter abundance,kerogen type,thermal maturity,mineral composition,diagenesis),the properties of fluids in pores(water,CH_(4),non-hydrocarbon gases),and geological conditions(temperature,pressure,preservation conditions)of the shale *** a particular basin or block,it is still challenging to define the main controlling factors,screen favorable exploration areas,and locate sweet *** to marine shales with extensive research and exploration data,lacustrine and marine-continental transitional shales are a further expanding area of *** methods have been developed to quantitatively characterize the in-situ gas content of shales,but all these methods have their own limitations,and more in-depth studies are needed to accurately evaluate and predict the in-situ gas content of shales,especially shales at deep depth.

关键词： Shale gas Retention mechanism Multi-component adsorption Influencing factors Evaluation method

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Laboratory Detection and Diagnosis of Filoviruses

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Virologica Sinica 2011年第2期26卷 73-80页

作者： Yun-peng Wang xian-en Zhang Hong-ping Wei State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071China

Ebola virus(EBOV) and Marburg virus(MARV),belonging to the Filoviridae family,emerged four decades ago and caused severe viral hemorrhagic fever in human and other *** high as 50-90% mortality,filoviruses can cause significant threats to public ***,so far no specific and efficient vaccine has been available,nor have other treatment methods proved to be *** is of great importance to detect these pathogens specific,rapidly and sensitively in order to control future filovirus ***,recent progresses in the development of detection and diagnosis methods for EBOV and MARV are summarized.

关键词： Filovirus Ebola virus (EBOV) Marburg virus (MARV) enzyme-linked immunosorbent assay(ELISA) Polymerase chain reaction (PCR)

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Prognostic and pathological impact of tumor budding in gastric cancer: A systematic review and meta-analysis

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World Journal of Gastrointestinal Oncology 2019年第10期11卷 898-908页

作者： Yi-xian Guo Zi-Zhen Zhang Gang Zhao en-Hao Zhao Department of Gastrointestinal Surgery Renji Hospital Shanghai Jiao Tong University School of Medicine

BACKGROUND Tumor budding,is a promising prognostic hallmark in many cancers,and can help us better assess the degree of malignancy in gastric cancer(GC)and in colorectal *** the past few years,several articles on the relationship between tumor budding and GC have been published,but different results have been *** the relationship between tumor budding and GC remains controversial,we integrated the data from 7 eligible studies to conduct a systematic review and *** To systematically evaluate the prognostic and pathological impact of tumor budding in *** Literature searches were conducted in the PubMed,Cochrane Library,EMBASE and Web of Science databases,and 7 cohort studies involving 2178 patients met our criteria and included in the *** patients were divided into those with high-grade tumor budding and those with low-grade tumor budding,and the cut-off values for tumor budding varied across the included *** hazard ratios(HRs)with 95%confidence intervals(CIs)were calculated to estimate the impact of tumor budding on overall survival(OS)in GC *** odds ratios(ORs)with 95%CIs were used to determine the correlation between tumor budding and pathological parameters(tumor stage,tumor differentiation,lymphovascular invasion,lymph node metastasis)of *** Seven studies involving 2178 patients were included in the *** combined ORs suggested that high-grade tumor budding was significantly associated with tumor stage(OR=6.63,95%CI:4.01-10.98,Pentiation(OR=3.74,95%CI:2.68-5.22,Pents with GC and an adverse 5-year OS(HR=1.93,95%CI:1.45-2.42,Pents with intestinal-type *** High-grade tumor budding suggested a poor prognosis in patients with GC o

关键词： Tumor budding Gastric cancer Intestinal-type gastric cancer Epithelialmesenchymal transition

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Phosphorus fertilization alters complexity of paddy soil dissolved organic matter

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Journal of Integrative Agriculture 2020年第9期19卷 2301-2312页

作者： ZHANG Zhi-jian WANG xian-zhe LIANG Lu-yi HUANG en TAO Xing-hua College of environmental and Resource Science Zhejiang UniversityHangzhou 310058P.R.China China Academy of West Region Development Zhejiang UniversityHangzhou 310058P.R.China Hangzhou Gusheng Agricultural Technology Company Limited Hangzhou 311108P.R.China

The structural complexity of soil dissolved organic matter(DOM)may reflect soil biogeochemical processes due to its spectral ***,the features of DOM structural complexity in paddy soil amended with long-term chemical P fertilization are still unclear,which may limit understanding of nutrient-related soil C *** collected soil samples from field experiments receiving application of 0,30,60,and 90 kg P ha–1 yr–1 to assess the effect of exogenous P on the complexity of soil DOM ***-dimensional excitation-emission matrix fluorescence analysis and enzymatic activity assay were used to determine the features of soil DOM molecular structure and the associated microbial *** results showed that P input increased the biodegradability of DOM,indicating by the increased lower molecular weight components and decreased humic degree in the DOM.P input also reduced the structural complexity of DOM with blue shifts of fluorescent *** fluorescence index andβ/αindex of DOM increased with increasing P application by 4–5%and 3–11%,respectively,while humification index decreased by 8–13%.The P input increased the abundance of bacteria and fungi by 34–167%and 159–964%,respectively,while 29–54%increments were found for theβ-1,4-glucosidase *** results implicated that P fertilization accelerated the soil DOM cycle,although the structural complexity of DOM declined,which potentially benefits soil C sequestration in paddy fields and may be a C sequestration mechanism in the P-dependent paddy.

关键词： soil carbon fertilization fluorescence humification agroecosystem

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Equilibrium Segregation to Free Surface:Kinet ics and Experiment

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Chinese Physics Letters 1999年第5期16卷 364-366页

作者： CAO Ze-xian Ralf Mueller WANG en-ge NIE Yu-xin State Key Laboratory for Surface Physics Institute of PhysicsChinese Academy of SciencesBeijing 100080 Department of Physics University Kaiserslautern67653 KaiserslauternGermany

Fick's first law is modified with an additional term df(x)/dx to describe the segregation phenomenon,f(x)is a local,analytical function related to the depth profile at *** thus can be determined experimentally by depth profiling *** starting in an initially homogeneous solid is described by an inhomogeneous diffusion equation on the half-infinite *** with and without formation of overlayer suggests different boundary *** solution for the Ansatz f(x)=as exp(-x/d_(s))is in perfect agreement with experimental results on sulphur segregation to the surface of *** from the detailed kinetics,activation energy can be inferred from the surface concentration evolution under different *** sulphur segregation in titanium,E_(act)=718 meV/atom.

关键词： technique. titanium sulphur

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中药材种植中植物生长调节剂的使用现状和应对措施

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中国新药杂志 2024年第14期33卷 1429-1436页

作者：安晓静刘尚年郭皓李先恩王彦婷关敏郭宝林陈彩霞中国医学科学院北京协和医学院药用植物研究所/中草药物质基础与资源利用教育部重点实验室/濒危药材繁育国家工程实验室北京100193 天津中医药大学中药学院天津301617 成都中医药大学药学院西南特色中药资源国家重点实验室成都611137 河北农业大学农学院保定071000

植物生长调节剂(plant growth regulator,PGR)是一类具有植物激素活性的人工合成的化合物,能通过调控植物生长发育、形态建成及物质代谢等,提高中药材产量和抗逆性。随着中药材种植品种和面积的增加,PGR在中药材种植生产中应用愈加广泛... 详细信息

植物生长调节剂(plant growth regulator,PGR)是一类具有植物激素活性的人工合成的化合物,能通过调控植物生长发育、形态建成及物质代谢等,提高中药材产量和抗逆性。随着中药材种植品种和面积的增加,PGR在中药材种植生产中应用愈加广泛。由于种植户对PGR缺乏充分认识和科学系统了解,使用时存在为追求产量盲目增加PGR种类和用量,导致药材品质下降等盲目乱用现象。本文通过对中药材主产区PGR使用现状进行调研,并结合近年来相关研究的文献资料,对中药材生产中PGR应用现状进行归纳、总结,分析中药材生产中PGR存在的问题,并提出可行建议和应对措施,以期为中药材生产实践中PGR应用提供技术指导和理论依据。

关键词：中药材植物生长调节剂使用现状应对措施

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Ratiometric fluorescence detection of bleomycin based on proximity-dependent fluorescence conversion of DNA-templated silver nanoclusters

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Chinese Chemical Letters 2021年第2期32卷 906-909页

作者： Lingyuan Liu Shuyun Zhu Jing Sun Meng Xia xian’en Zhao Guobao Xu College of Chemistry and Chemical engineering Qufu Normal UniversityQufu 273165China Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources Northwest Institute of Plateau BiologyChinese Academy of SciencesXining 810001China State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China

We design a ratiometric fluo rescent sensing platform for bleomycin(BLM) by using proximity-dependent DNA-templated silver nanoclusters(DNA-AgNCs) *** ratiometric sensing system is constructed with DNA-AgNCs as single *** proposed strategy is based on the two following facts:(1) a covert DNA can approach and transform the DNA-AgNCs with green emission(G-DNA-AgNCs) into red emission through hybridization reaction.(2) The specific cleavage of the convert DNA by BLM in the presence of Fe(Ⅱ) inhibits the discoloration of ***,benefiting from the specific recognition of BLM and unique properties of G-DNA-AgNCs,a hignly-sensitive ratiometric sensor for BLM has been successfully *** detection limit is as low as 30 pmol/*** label-free fluorescence probe possesses advantages of convenient synthetic process and low ***,this ratiometric method has been applied to the detection of BLM in human serum samples,illustrating a promising tool for analysis of BLM in cancer therapy.

关键词： Ratiometric nanosensor DNA-templated silver nanoclusters Fluorescence transformation Bleomycin DNA scission

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A switch-on molecular biosensor for detection of caspase-3 and imaging of apoptosis of cells

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Science China(Life Sciences) 2022年第3期65卷 540-549页

作者： Rui Gong Dianbing Wang Ghulam Abbas Shimin Li Qian Liu Mengmeng Cui xian-en Zhang National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Faculty of Synthetic Biology Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China

Apoptosis is a form of programmed cell death that is essential for maintaining internal environmental *** apoptosis can cause a variety of diseases;therefore,sensing apoptosis can provide help in study of mechanism of the relevant diseases and drug *** is known that caspase-3 is a key enzyme involved in apoptosis and the expression of its activity is an indication of ***,we present a genetically encoded switch-on m Neon Green2-based molecular biosensor.m Neon Green2 is the brightest monomeric green fluorescent *** substrate of caspase-3,DEVD amino acid residues,is inserted in it,while cyclized by insertion of Nostoc punctiforme Dna E intein to abolish the fluorescence(inactive state).Caspase-3-catalyzed cleavage of DEVD linearizes m Neon Green2 and rebuilds the natural barrel structure to restore the fluorescence(activated state).The characterization exhibited that the Caspase-3 biosensor has shortened response time,higher sensitivity,and prolonged functional shelf life in detection of caspase-3 amongst the existing *** also used the Caspase-3 biosensor to evaluate the effect of several drugs on the induction of apoptosis of He La and MCF-7 tumor cells and inhibition of Zika virus invasion.

关键词： apoptosis caspase-3 molecular biosensor molecular imaging Zika virus

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Detection of let-7a microRNA by real-time PCR in gastric carcinoma

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World Journal of Gastroenterology 2007年第20期13卷 2883-2888页

作者： Hong-He Zhang xian-Jun Wang Guo-Xiong Li en Yang Ning-Min Yang Diagnostic Laboratory Hangzhou First People's Hospital Hangzhou 310006 Zhejiang Province China Zhiyuan Medical Laboratory Hangzhou 310006 Zhejiang Province China

AIM: To establish an accurate and rapid stem-loop reverse transcriptional real-time PCR (RT-PCR) method to quantify human let-7a miRNA in gastric cancer. METHODS: According to the sequence of let-7a miRNA,the stem-loop reverse transcriptional primer,the primers and quantitative MGB probes of real-time PCR were designed and synthesized. The dynamic range and the sensitivity of quantitative reverse transcriptional real-time PCR were determined. The levels of let-7a miRNA were examined in 32 gastric carcinoma samples by stem-loop RT-PCR method. RESULTS: The dynamic range and sensitivity of the let-7a miRNA quantification scheme were evaluated,the result showed the assay could precisely detect 10 copies of mature let-7a miRNA in as few as 0.05 ng of total RNA of gastric mucosa. The results of specificity analysis showed no fluorescence signal occurred even though 50 ng of human genomic DNA was added to the reverse transcription (RT) reaction. The expression level of let-7a miRNA in gastric tumor tissues was significantly lower compared to normal tissues in 14 samples from 32 patients. CONCLUSION: The stem-loop RT-PCR is a reliable method to detect let-7a miRNA which may play an important role in the development of gastric carcinoma.

关键词： MicroRNA Let-7a Real-time PCR Gastric carcinoma

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Temporal transcriptomics reveal the molecular mechanism of dormancy and germination regulated by temperature in Paris polyphylla seed

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Horticultural Plant Journal 2023年第4期9卷 848-866页

作者： Dengqun Liao Yalan Chen Jianjun Qi Hongliang Zhang Peng Sun Caixia Chen xian'en Li Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100193China Key Lab of Crop Heterosis and Utilization of Ministry of Education Beijing Key Lab of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China

The mature seed of Paris polyphylla ***(PPC)is morphophysiologically dormant and develops differently under warm and cold *** elucidate the molecular mechanisms underlying temperature-dependent regulation of PPC seed dormancy and germination,we investigated the dynamic changes in PPC seed transcript levels under warm and cold temperature stratifications(WS and CS,respectively)by time-resolved RNA sequencing,focusing on genes related to hormone metabolism and signaling and cell wall remodeling(CWRM)and encoding transcription factors/regulators(TFs/TRs).A total of 48765 and 47836 differentially expressed genes(DEGs)were associated with WS and CS,*** these,17581 and 16652 DEGs,respectively,unique to WS and CS,and 5386 were common to both temperature stratifications across nine analyzed growth *** expression of hormone metabolism and signaling,TF/TR,and CWRM genes were closely associated with *** genes related to gibberellin(GA),cytokinin,auxin,and brassinosteroid biosynthetic were upregulated in WS as compared to CS seeds,while genes related to dormancy release and germination were downregulated in WS ***,only GA and abscisic acid levels were altered in PPC seeds breaking morphophysiological dormancy(MPD).Overall,37 TF and five TR families were upregulated whereas 24 TF and 16 TR families were downregulated in WS as compared to CS *** CWRM families were highly expressed under WS as compared to CS,suggesting that they promote endosperm weakening and embryo growth of WS seeds and facilitate MPD release and germination.A coexpression analysis revealed positive correlations between TFs/TRs and DEGs involved in hormone metabolism and signaling and *** results provided a basis for investigating the interaction between the endosperm and underdeveloped embryo in the regulation of PPC seed germination and seedling emergence.

关键词： Paris polyphylla var.chinensis Morphophysiologically dormant seed Temperature stratification Time transcriptome Phytohormone Transcription factor Cell wall remodeling gene

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