Ratiometric fluorescence detection of bleomycin based on proximity-dependent fluorescence conversion of DNA-templated silver nanoclusters

作     者：Lingyuan Liu Shuyun Zhu Jing Sun Meng Xia Xian’en Zhao Guobao Xu Lingyuan Liu;Shuyun Zhu;Jing Sun;Meng Xia;Xian'en Zhao;Guobao Xu

作者机构：College of Chemistry and Chemical EngineeringQufu Normal UniversityQufu 273165China Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological ResourcesNorthwest Institute of Plateau BiologyChinese Academy of SciencesXining 810001China State Key Laboratory of Electroanalytical ChemistryChangchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2021年第32卷第2期

页      面：906-909页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China (No.21775088) 

主　　题：Ratiometric nanosensor DNA-templated silver nanoclusters Fluorescence transformation Bleomycin DNA scission 

摘      要：We design a ratiometric fluo rescent sensing platform for bleomycin(BLM) by using proximity-dependent DNA-templated silver nanoclusters(DNA-AgNCs) *** ratiometric sensing system is constructed with DNA-AgNCs as single *** proposed strategy is based on the two following facts:(1) a covert DNA can approach and transform the DNA-AgNCs with green emission(G-DNA-AgNCs) into red emission through hybridization reaction.(2) The specific cleavage of the convert DNA by BLM in the presence of Fe(Ⅱ) inhibits the discoloration of ***,benefiting from the specific recognition of BLM and unique properties of G-DNA-AgNCs,a hignly-sensitive ratiometric sensor for BLM has been successfully *** detection limit is as low as 30 pmol/*** label-free fluorescence probe possesses advantages of convenient synthetic process and low ***,this ratiometric method has been applied to the detection of BLM in human serum samples,illustrating a promising tool for analysis of BLM in cancer therapy.