检索结果-南通市图书馆

Wharton’s jelly mesenchymal stem cells: Future regenerative medicine for clinical applications in mitigation of radiation injury

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

World Journal of Stem Cells 2024年第7期16卷 742-759页

作者： Prashasti Sharma dharmendra kumar Maurya Life Sciences Homi Bhabha National InstituteMumbai 400094MaharashtraIndia Radiation Biology&Health Sciences Division Bhabha Atomic Research CentreMumbai 400085MaharashtraIndia

Wharton’s jelly mesenchymal stem cells(WJ-MSCs)are gaining significant attention in regenerative medicine for their potential to treat degenerative diseases and mitigate radiation ***-MSCs are more naïve and have a better safety profile,making them suitable for both autologous and allogeneic *** review highlights the regenerative potential of WJ-MSCs and their clinical applications in mitigating various types of radiation *** this review,we will also describe why WJ-MSCs will become one of the most probable stem cells for future regenerative medicine along with a balanced view on their strengths and ***,the most updated literature related to both preclinical and clinical usage of WJ-MSCs for their potential application in the regeneration of tissues and organs will also be compiled.

关键词： Stem cells Wharton’s jelly mesenchymal stem cells Radiotherapy Xerostomia Lung fibrosis

Cocktails of defined chemical compounds:sufficient to induce totipotency in embryonic stem cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2022年第10期7卷 3630-3631页

作者： Wilfried A.Kues dharmendra kumar Friedrich-Loeffler-Institut Federal Research Institute for Animal HealthBiotechnologyStem Cell Physiology31535 NeustadtGermany Animal Physiology and Reproduction Division ICAR-Central Institute for Research on BuffaloesHisarHaryana 125001India

In a recent work published in Nature,Hu et al.1 report that a cocktail of three small chemicals is sufficient to induce totipotency in murine embryonic stem(ES)*** this year,two other groups identified different mixtures of chemicals,which also seem to do this job,2,3 taken together this opens a new approach to capture totipotency in vitro,and to understand the underlying pathways and their interaction.

关键词： interaction. al. induce

Soluble factors secreted by human Wharton’s jelly mesenchymal stromal/stem cells exhibit therapeutic radioprotection: A mechanistic study with integrating network biology

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Stem Cells 2022年第5期14卷 347-361页

作者： dharmendra kumar Maurya Mayuri Bandekar Santosh kumar Sandur Radiation Biology and Health Sciences Division Bhabha Atomic Research CentreMumbai 400085MaharashtraIndia Homi Bhabha National Institute AnushaktinagarMumbai 400094India University of Mumbai KalinaMumbai 400098India

BACKGROUND Human Wharton’s jelly-derived mesenchymal stromal/stem cells(hWJ-MSCs)have gained considerable attention in their applications in cell-based therapy due to several advantages offered by ***,we reported that hWJ-MSCs and their conditioned medium have significant therapeutic radioprotective *** finding raised an obvious question to identify unique features of hWJ-MSCs over other sources of stem cells for a better understanding of its radioprotective *** To understand the radioprotective mechanism of soluble factors secreted by hWJMSCs and identification of their unique *** Propidium iodide staining,endogenous spleen colony-forming assay,and survival study were carried out for radioprotection ***-driven proliferation assay was performed for in vivo lymphocyte *** of RNAseq data was performed to find the unique genes of WJ-MSCs by comparing them with bone marrow mesenchymal stem cells,embryonic stem cells,and human *** enrichment analysis and protein-protein interaction network were used for pathway *** Co-culture of irradiated murine splenic lymphocytes with WJ-MSCs offered significant radioprotection to ***-MSC transplantation increased the homeostasis-driven proliferation of the *** of WJ-MSC conditioned medium with granulocyte-colony stimulating factor antibody abolished therapeutic *** analysis showed that WJ-MSCs share several common genes with bone marrow MSCs and embryonic stem cells and express high levels of unique genes such as interleukin(IL)1-α,IL1-β,IL-6,CXCL3,CXCL5,CXCL8,CXCL2,CCL2,FLT-1,and *** was also observed that WJ-MSCs preferentially modulate several cellular pathways and processes that handle the repair and regeneration of damaged tissues compared to stem cells from other ***-based network analysis showed that most of the radiosensitive tissues have a more com

关键词： Radioprotection Mesenchymal stem cells Wharton’s jelly-derived mesenchymal stromal/stem cells Cytokines Granulocyte-colony stimulating factor Network biology

Immunochemical characterization of antigens of Brucella canis and their use in seroprevalence study of canine brucellosis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Medicine 2011年第11期4卷 857-861页

作者： Sharma Barkha Sinha dharmendra kumar Singh Dhirendra kumar Department of Epidemiology and Preventive Medicine College of Veterinary SciencePandit Deen Dayal Upadhaya Veterinary UniversityMathuraUttar PradeshPin-281001India Division of Epidemiology Indian Veterinary Research InstituteIzatnagarBareillyUttar Pradesh243122India Division of Veterinary Public Health Indian Veterinary Research InstituteIzatnagarBareillyUttar Pradesh243122 India

Objective:To explore immunochemical characterization of antigens of Brucella canis(B. canis),and the use in seroprevalence study of canine ***:External hot phosphate buffer saline extract(HPBSE) and internal sonicated(SA) antigens were prepared from *** strain MEX 51 and imniunochemically *** antigens were used to test 527 serum samples of dogs by 2-mercaptoethanol-tubc agglutination test(2 ME-TAT), agar gel immunodiffusion test(AGID).dot-ELISA and indirect enzyme-linked immunosorbent assay(I-ELISA) to assess the seroprevalence of canine ***:The protein content of HPBSE and SA antigens was 0.387 mg/*** 0.195 mg/mL,respectively,whereas carbohydrate content was 0.174 mg/mL and 0.150 mg/mL,*** sodium dodecyl sulfate-polyacrylamide gel electrophoresis(12.5%) of HPBSE and SA,revealed 6 and 8 visible peptide bands ranging from 18-80 kDa and 12-45 kDa,*** blot analysis showed immunodominant bands of MW 12.28.39 and 45 kl)a for HPBSE and 20-24 kl)a for SA. The AGII) revealed HPBSE as more specific antigen than SA but both I-ELISA and dot-ELISA indicated SA antigen to be more specific and reliable than *** seroprevalence of canine brucellosis was 2.27%by 2ME-TAT.1.5%by AGID.3.03%by dot-ELISA and 16.12%by I-ELISA. Conclusions:On the basis of the results of present study,we concluded that HPBSE is suitable antigen for AGID,which is more specific:whereas SA antigen is suitable for I-ELISA,which is highly ***,initial screening of serum samples should be carried out by I-ELISA followed by confirmation with AGID.

关键词： Brucella canis SDS-PAGE Western blotting Agar gel immunodiffusion test Dot-ELISA I-ELISA

Potential of transposon-mediated cellular reprogramming towards cell-based therapies

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Stem Cells 2020年第7期12卷 527-544页

作者： dharmendra kumar Taruna Anand Thirumala R Talluri Wilfried A Kues Animal Physiology and Reproduction Division ICAR-Central Institute for Research on BuffaloesHisar 125001India NCVTC ICAR-National Research Centre on EquinesHisar 125001India Equine Production Campus ICAR-National Research Centre on EquinesBikaner 334001India Friedrich-Loeffler-Institut Institute of Farm Animal GeneticsDepartment of BiotechnologyMariensee 31535Germany

Induced pluripotent stem(iPS)cells present a seminal discovery in cell biology and promise to support innovative treatments of so far incurable *** translate iPS technology into clinical trials,the safety and stability of these reprogrammed cells needs to be *** recent years,different non-viral transposon systems have been developed for the induction of cellular pluripotency,and for the directed differentiation into desired cell *** this review,we summarize the current state of the art of different transposon systems in iPS-based cell therapies.

关键词： Transposons Induced pluripotent stem cells Clinical applications Cellular reprogramming Cell-based therapy Genetic correction

Induced pluripotent stem cells: Mechanisms, achievements and perspectives in farm animals

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Stem Cells 2015年第2期7卷 315-328页

作者： dharmendra kumar Thirumala R Talluri Taruna Anand Wilfried A Kues Institute of Farm Animal Genetics Friedrich-Loeffler-Institute Animal Physiology and Reproduction Division Central Institute for Research on Buffaloes National Research Centre on Equines

Pluripotent stem cells are unspecialized cells withunlimited self-renewal, and they can be triggered to differentiate into desired specialized cell types. These features provide the basis for an unlimited cell source for innovative cell therapies. Pluripotent cells also allow to study developmental pathways, and to employ them or their differentiated cell derivatives in pharmaceutical testing and biotechnological applications. Via blastocyst complementation, pluripotent cells are a favoured tool for the generation of genetically modified mice. The recently established technology to generate an induced pluripotency status by ectopic co-expression of the transcription factors Oct4, Sox2, Klf4 and c-Myc allows to extending these applications to farm animal species, for which the derivation of genuine embryonic stem cells was not successful so far. Most induced pluripotent stem(i PS) cells are generated by retroviral or lentiviral transduction of reprogramming factors. Multiple viral integrations into the genome may cause insertional mutagenesis and may increase the risk of tumour formation. Non-integration methods have been reported to overcome the safety concerns associated with retro and lentiviral-derived i PS cells, such as transient expression of the reprogramming factors using episomal plasmids, and direct delivery of reprogramming m RNAs or proteins. In this review, we focus on the mechanisms of cellular reprogramming and current methods used to induce pluripotency. We also highlight problems associated with the generation of i PS cells. An increased understanding of the fundamental mechanisms underlying pluripotency and refining the methodology of i PS cell generation will have a profound impact on future development and application in regenerative medicine and reproductive biotechnology of farm animals.

关键词： Reprogramming Large animal models Stemness Chimera Germline transmission Inducedpluripotent stem cells Gene delivery

Perspectives of pluripotent stem cells in livestock

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Stem Cells 2021年第1期13卷 1-29页

作者： dharmendra kumar Thirumala R Talluri Naresh L Selokar Iqbal Hyder Wilfried A Ku Animal Physiology and Reproduction Division ICARCentral Institute for Research on BuffaloesHisar 125001India Equine Production Campus ICAR-National Research Centre on EquinesBikaner 334001India Department of Physiology NTR College of Veterinary ScienceGannavaram 521102India Department of Biotechnology Friedrich-Loeffler-InstituteFederal Institute of Animal HealthNeustadt 31535Germany

The recent progress in derivation of pluripotent stem cells(PSCs)from farm animals opens new approaches not only for reproduction,genetic engineering,treatment and conservation of these species,but also for screening novel drugs for their efficacy and toxicity,and modelling of human *** attempts to derive PSCs from the inner cell mass of blastocyst stages in farm animals were largely unsuccessful as either the cells survived for only a few passages,or lost their cellular potency;indicating that the protocols which allowed the derivation of murine or human embryonic stem(ES)cells were not sufficient to support the maintenance of ES cells from farm *** scenario changed by the innovation of induced pluripotency and by the development of the 3 inhibitor culture conditions to support naïve pluripotency in ES cells from livestock ***,the long-term culture of livestock PSCs while maintaining the full pluripotency is still challenging,and requires further ***,we review the current achievements in the derivation of PSCs from farm animals,and discuss the potential application areas.

关键词： Livestock Cellular reprogramming Chimera Cell-therapy Ontogenesis Pluripotency

Modelling the potential risk zone of Lantana camara invasion and response to climate change in eastern India

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ecological Processes 2022年第1期11卷 142-154页

作者： Sharad Tiwari S.N.Mishra dharmendra kumar Basant kumar S.N.Vaidya Brojo Gopal Ghosh Sk Mujibar Rahaman Masjuda Khatun Sanjoy Garai Amit kumar Institute of Forest Productivity LalgutwaRanchi 835303JharkhandIndia Department of IT Govt.of JharkhandJharkhand Space Application CentreRanchiIndia Department of Geoinformatics Central University of JharkhandRanchi 835205JharkhandIndia

Background:The aim of this study is to elucidate the potential risk zones prone to the invasion of perennial ornamental plant Lantana camara,which is native to South America and has invasive tendency in Jharkhand,eastern India,for present(2020)and the future(2050)climatic conditions under four different Representative Concentration Pathways scenarios(RCPs).We analysed the current distribution pattern of *** in the plateau region of eastern India and identified potentially suitable habitats prone to its further infestation in the future under the climate change ***:Results showed the presence of *** invasion in~13%of the geographical area of Jharkhand,Cho-tanagpur plateau which may expand up to 20–26%by 2050 depending upon emission scenarios as characterised by the four *** for the current scenario suggests the dominance of *** in sub-zone V(12.77%under high risk zones(HRZ)and 9.5%under critical risk zones(CRZ))followed by sub-zones IV(6.7%:HRZ;4.19%:CRZ)and VI(2.49%:HRZ;2.14%:CRZ).Future projection(2050)indicates a possible expansion of its distribution range across all agro-climatic sub-zones with dominance in sub-zones V and *** Bio_4(temperature seasonality)was observed as the most contributing factor for the distribution of *** for current and future scenarios across all *** habitat for *** mostly occurred under natural vegetation(66.05%of CRZ and 60.71%of HRZ)and agriculture landscape(29.51%of CRZ and 34.48%of HRZ).Conclusions:The study provides an insight of invasion of *** in the plateau region of eastern India,and reveals wide distribution across all the agro-climatic sub-zones of Jharkhand,mostly in open and disturbed areas under natural vegetation and agriculture *** projections for the year 2050 suggest a continuous increase in the expansion range of invasion across Jharkhand and call for urgent initiatives to combat its further invasion.

关键词： Climate change Forest Invasion Lantana camara Maxent RCPs

Microtubule acetylation dyshomeostasis in Parkinson’s disease

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Translational Neurodegeneration 2023年第1期12卷 576-600页

作者： Padmashri Naren Khan Sabiya Samim Kamatham Pushpa Tryphena Lalitkumar K.Vora Saurabh Srivastava dharmendra kumar Khatri Shashi Bala Singh Molecular and Cellular Neuroscience Lab Department of Pharmacology and ToxicologyNational Institute of Pharmaceutical Education and Research(NIPER)HyderabadTelangana 500037India School of Pharmacy Queen’s University Belfast97 Lisburn RoadBelfast BT97BLUK Department of Pharmaceutics National Institute of Pharmaceutical Education and Research(NIPER)HyderabadTelangana 500037India

The inter-neuronal communication occurring in extensively branched neuronal cells is achieved primarily through the microtubule(MT)-mediated axonal transport *** mechanistically regulated system delivers cargos(proteins,mRNAs and organelles such as mitochondria)back and forth from the soma to the *** proteins like kinesins and dynein mechanistically regulate polarized anterograde(from the soma to the synapse)and retrograde(from the synapse to the soma)commute of the cargos,*** axonal transport of such cargos is achieved by altering the microtubule stability via post-translational modifications(PTMs)ofα-andβ-tubulin heterodimers,core components constructing the *** within the lumen of MTs,K40 acetylation ofα-tubulin viaα-tubulin acetyl transferase and its subsequent deacetylation by HDAC6 and SIRT2 are widely scrutinized PTMs that make the MTs highly flexible,which in turn promotes their *** movement of various motor proteins,including kinesin-1(responsible for axonal mitochondrial commute),is enhanced by this PTM,and dyshomeostasis of neuronal MT acetylation has been observed in a variety of neurodegenerative conditions,including Alzheimer’s disease and Parkinson’s disease(PD).PD is the second most common neurodegenerative condition and is closely associated with impaired MT dynamics and deregulated tubulin acetylation *** the relationship between status of MT acetylation and progression of PD pathogenesis has become a chicken-and-egg question,our review aims to provide insights into the MT-mediated axonal commute of mitochondria and dyshomeostasis of MT acetylation in *** enzymatic regulators of MT acetylation along with their synthetic modulators have also been briefly *** towards a tubulin-based therapy that enhances MT acetylation could serve as a disease-modifying treatment in neurological conditions that lack it.

关键词： Parkinson’s disease Microtubule acetylation Tubulin PTMs Mitochondria Axonal transport HDAC6 SIRT2 inhibitors