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Mineralized collagen scaffolds fabricated with amniotic membrane matrix increase osteogenesis under inflammatory conditions

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Regenerative Biomaterials 2020年第3期7卷 247-258页

作者： Marley j.Dewey Eileen M.j.hnson Simona T.Slater derek j.Milner Matthew B.Wheeler Brendan A.C.Harley Department of Materials Science and Engineering University of Illinois at Urbana-Champaign110 Roger Adams Laboratory600 S.Mathews Ave.UrbanaIL 61801USA Department of Bioengineering University of Illinois at Urbana-Champaign110 Roger Adams Laboratory600 S.Mathews Ave.UrbanaIL 61801USA Department of Chemical and Biomolecular Engineering University of Illinois at Urbana-Champaign110 Roger Adams Laboratory600 S.Mathews Ave.UrbanaIL 61801USA Carl R.Woese Institute for Genomic Biology University of Illinois at Urbana-Champaign110 Roger Adams Laboratory600 S.Mathews Ave.UrbanaIL 61801USA Department of Animal Sciences University of Illinois at Urbana-Champaign110 Roger Adams Laboratory600 S.Mathews Ave.UrbanaIL 61801USA

Defects in craniofacial bones occur congenitally,after high-energy impacts,and during the course of treatment for stroke and *** inj.ries are difficult to heal due to the overwhelming size of the inj.ry area and the inflammatory environment surrounding the *** inflammatory response after inj.ry may greatly inhibit regenerative *** have developed mineralized collagen scaffolds that can induce osteogenic differentiation and matrix biosynthesis in the absence of osteogenic media or supplemental *** amniotic membrane is derived from placentas and has been recently investigated as an extracellular matrix to prevent chronic ***,we hypothesized that a mineralized collagen-amnion composite scaffold could increase osteogenic activity in the presence of inflammatory *** report mechanical properties of a mineralized collagen-amnion scaffold and investigated osteogenic differentiation and mineral deposition of porcine adipose-derived stem cells within these scaffolds as a function of inflammatory *** of amniotic membrane matrix promotes osteogenesis similarly to un-modified mineralized collagen scaffolds,and increases in mineralized collagen-amnion scaffolds under inflammatory ***,these findings suggest that a mineralized collagen-amnion scaffold may provide a beneficial environment to aid craniomaxillofacial bone repair,especially in the course of defects presenting significant inflammatory complications.

关键词： mineralized collagen scaffolds amniotic membrane inflammation stem cell osteogenesis

Error modeling, sensitivity analysis and assembly process of a class of 3-DOF parallel kinematic machines with parallelogram struts

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Science China(Technological Sciences) 2002年第5期45卷 467-476页

作者：黄田李亚唐国宝李思维赵兴玉 David.j.Whitehouse derek G.Chetewyn Xianping Liu School of Mechanical Engineering Tianjin University Tianjin 300072 China

This paper presents an error modeling methodology that enables the tolerance design, assembly and kinematic calibration of a class of 3-DOF parallel kinematic machines with parallelogram struts to be integrated into a unified framework. The error mapping function is formulated to identify the source errors affecting the uncompensable pose error. The sensitivity analysis in the sense of statistics is also carried out to investigate the influences of source errors on the pose accuracy. An assembly process that can effectively minimize the uncompensable pose error is proposed as one of the results of this investigation.

关键词： parallel kinematic machine, error modeling, sensitivity analysis, assembly process.

Menaquinone (Vitamin K2) Enhancement of <i>Staphylococcus aureus</i>Biofilm Formation

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j.urnal of Biosciences and Medicines 2014年第1期2卷 26-32页

作者： derek T. Kirby j.yce M. Savage Balbina j. Plotkin Department of Microbiology and Immunology Midwestern University Downers Grove USA

During infection, Staphylococcus aureus is exposed to exogenous menaquinone which is essential for the human blood clotting cascade. The effect of exogenous menaquinone on S. aureus phenotypic expression is not known. To test whether menaquinone affects expression of virulence-associated phenotypes, methicillin-sensitive (MSSA) and -resistant (MRSA) S. aureus strains (n = 8) were grown in the presence of menaquinone (0.001 - 12 μg/ml). Capsule production, biofilm formation (plastic and fibronectin-coated microtiter plates) and carotenoid levels were determined spectrophotometrically after growth in Mueller Hinton broth (MH;24-hr, 37°C). All experiments were, at minimum, done in triplicate and repeated twice. Menaquinone at physiologic levels (0.01 μg/ml MH) significantly increased (p 0.05) biofilm formation on plastic in a manner that was bacterial population size dependent. In addition, menaquinone (0.05 - 4 μg/ml) significantly increased (p 0.05) biofilm formation on fibronectin-coated surfaces for four MSSA strains and one MRSA strain by two to six-fold as compared to medium controls. However, menaquinone had no effect on capsule production or cell-associated carotenoid levels. Menaquinone’s effect on biofilm formation on fibronectin-coated surfaces appears to be regulated by sarA. These findings are the first to demonstrate that a vitamin at concentrations reported in humans affects S. aureus virulence-associated phenotypes.

关键词： Quorum S. aureus Menaquinone Fibronectin Biofilm Capsule Plastic Adherence Carotenoid

Semi-Automated Method for Multi-Tasking Measurement of Microbial Growth, Capsule, and Biofilm Formation

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Advances in Microbiology 2012年第4期2卷 623-628页

作者： derek T. Kirby Craigory j. Raino Samuel F. Rabor j.. Cassandra j. Wasson Balbina j. Plotkin Department of Microbiology and Immunology Midwestern University Downers Grove USA Department of Microbiology and Immunology Midwestern University Downers Grove USA

The maj.rity of bacterial infections involve the formation of biofilms. Biofilm formation is nutrient and growth dependent. Determination of the effects of nutrients on exopolysaccharide production and bacterial growth is labor and time intensive. We tested whether the Bioscreen C (Growth Curves, Inc.) would have utility as a high-throughput tool in the measurement of fundamental phenotype expression, as it relates to growth conditions. Within 48 - 72 hr, reproduceble, statistically significant data on the affects of growth conditions on generation time, capsule production and biofilm formation (maximally for 25 different conditions per 24 hr run cycle;n = 4) were obtained. Although all S. aureus strains produced similar amounts of capsule, sarA– and agr– strains grew significantly slower than parent strain (1.6 fold slower) and produced significantly (p 0.05) less biofilm (~2 fold). E. coli growth rate, biofilm and capsule production in simulated nephropathic urine medium was similar for urine with insulin (20 μU). Addition of insulin to urine medium with proline increased generation time, capsule and biofilm production. Findings from this study show that the Bioscreen C is a rapid, reproducible, and easily manipulated system to concurrently measure bacterial growth, biofilm formation, and capsule production. In addition, there is the potential for further applications of this system by expanding the types of detector dyes used.

关键词： Biofilm Staphylococcus aureus Escherichia coli Quorum-Signaling Compounds Menaquinone Insulin Growth

Experience after 100 patients treated with cytoreductive surgery and hyperthermic intraperitoneal chemotherapy

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World j.urnal of Gastroenterology 2012年第17期18卷 2061-2066页

作者： Ingmar Knigsrainer derek Zieker j.rg Glatzle Olivia Lauk j.lia Klimek Stephan Symons Bj.rn Brücher Stefan Beckert Alfred Knigsrainer Department of Surgery University of TübingenHoppeSeyler-Strasse 3D-72076 TübingenGermany Center for Bioinformatics Tübingen Sand 14D-72076 TübingenGermany

AIM:To investigate perioperative patient morbidity/mortality and outcome after cytoreductive surgery(CRS) and hyperthermic intraperitoneal chemotherapy(HIPEC).METHODS:Of 150 patients 100 were treated with cytoreductive surgery and HIPEC and retrospectively *** and postoperative follow-up data were *** mass index(BMI),age and peritoneal carcinomatosis index(PCI) were chosen as selection criteria with regard to tumor-free survival and perioperative morbidity for this multimodal ***:CRS with HIPEC was successfully performed in 100 out of 150 *** patients were excluded because of intraoperative *** PCI was 17(1-39).In 89% a radical resection(CC0/CC1) was *** patient died postoperatively due to multiorgan *** PCI,age nor BMI was a risk factor for postoperative complications/outcome according to the DINDO *** 9% Re-CRS with HIPEC was performed during the follow-up ***:Patient selection remains the most important *** PCI,age nor BMI alone should be an exclusion criterion for this multimodal therapy.

关键词：肿瘤细胞热化疗治疗腔内选择标准多式联运 PCI CRS

Novel Identification of LYVE-1 Positive Macrophages in Rheumatoid Synovial Tissue

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Open j.urnal of Rheumatology and Autoimmune Diseases 2016年第3期6卷 74-78页

作者： Lisa j. M. Rump derek L. Mattey Oksana Kehoe j.m Middleton Keele University Arthritis Research Centre at the Robert Jones and Agnes Hunt Orthopaedic Hospital Foundation Trust Oswestry UK Institute for Science and Technology in Medicine Medical School Keele University Stoke-on-Trent UK Haywood Rheumatology Centre Haywood Hospital High Lane Burslem Stoke-on-Trent UK Faculty of Medicine and Dentistry School of Oral and Dental Sciences Bristol University Bristol UK

Obj.ctive: LYVE-1+ macrophages are observed in a range of cancers, where they play a role in tumour lymphangiogenesis. In rheumatoid arthritis (RA), lymphangiogenesis increases in the early stage of the disease and decreases as it progresses, potentially exacerbating inflammatory cell persistence. We investigated whether LYVE-1+ macrophages were present in RA synovium. Methods: Synovial tissue from RA patients was obtained at j.int replacement surgery and immunohistochemistry was performed to visualise LYVE-1+ and CD68+ cells. Results: LYVE-1+ macrophages were present in rheumatoid synovial tissue, the first observation of this kind. Conclusion: Despite the reduction in lymphangiogenesis in chronic RA, LYVE-1 positive macrophages are present and there is a potential role for macrophages in the generation of lymphatic vessels.

关键词： LYVE-1 Macrophage Rheumatoid Arthritis Endothelial Lymphatic

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管线钢夹杂物检测与分析

管线钢夹杂物检测与分析

HASTY modulates miRNA biogenesis by linking pri-miRNA transcription and processing

2007中国钢铁年会

作者： K. Holz j. Rossi Y.D. Yang A. mclean 多伦多大学材料科学与工程系多伦多大学材料科学与工程系多伦多大学材料科学与工程系多伦多大学材料科学与工程系

本文通过硫印检验法、改进的火花光谱分析法和 SPEED-SEM-EDX 方法研究了管线钢中夹杂物的形貌、成分和分布。分析步骤为：采用选择性衡电位腐蚀技术通过电解溶解方法制备金属样品,利用 SEM-EDX 设备观察、检测夹杂物。硫印检测和火花... 详细信息

本文通过硫印检验法、改进的火花光谱分析法和 SPEED-SEM-EDX 方法研究了管线钢中夹杂物的形貌、成分和分布。分析步骤为：采用选择性衡电位腐蚀技术通过电解溶解方法制备金属样品,利用 SEM-EDX 设备观察、检测夹杂物。硫印检测和火花光谱分析表明样品中出现了规则的中心线,其含硫含量很高,样品上部的硫含量比下部的高。SEM 结果表明样品中绝大部分的夹杂物都呈球形,约2～5μm 大小,硫和锰含量都很高。部分夹杂物含有 Al、Ca、Mn 和 S,呈现明亮球形,很可能形成了 MnS,CaS 和铝酸钙。结论表明硫印检测法是一种经济而方便的夹杂物检测手段,电花光谱分析是一种独立的、彻底的夹杂物检测手段,而 SPEED-SEM-EDX 则最适合现场检测和三维观测。

关键词：管线钢夹杂物检测偏析硫化物连铸氢致裂纹

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Molecular Plant 2021年第3期14卷 426-439页

作者： Damian A.Cambiagno Axel j.Giudicatti Agustin L.Arce Delfina Gagliardi Lei Li Wei Yuan derek S.Lundberg Detlef Weigel Pablo A.Manavella Instituto de Agrobiotecnologı´a del Litoral(CONICET-UNL) Ca´tedra de Biologı´a Celular y MolecularFacultad de Bioquı´mica y Ciencias Biolo´gicasUniversidad Nacional del Litoral3000 Santa FeArgentina Department of Molecular Biology Max Planck Institute for Developmental Biology72076 T€ubingenGermany

Post-transcriptional gene silencing mediated by microRNAs(miRNAs)modulates numerous developmental and stress response *** the last two decades,HASTY(HST),the ortholog of human EXPORTIN 5,was considered to be a candidate protein that exports plant miRNAs from the nucleus to the ***,we report that HST functions in the miRNA pathway independent of its cargo-exporting activity in *** found that Arabidopsis mutants with impaired HST shuttling exhibit normal subcellular distribution of ***,protein-protein interaction and microscopy assays showed that HST directly interacts with the microprocessor core component DCL1 through its N-terminal ***,mass spectrometry analysis revealed that HST also interacts independently of its N-terminal domain with the mediator complex subunit *** experiments revealed that HST could act as a scaffold to facilitate the recruitment of DCL1 to genomic MIRNA loci by stabilizing the DCL1-MED37 complex,which in turn promotes the transcription and proper processing of primary miRNA transcripts(primiRNAs).Taken together,these results suggest that HST is likely associated with the formation of the miRNA biogenesis complex at MIRNA genes,promoting the transcription and processing of primiRNAs rather than the direct export of processed miRNAs from the nucleus.

关键词： microRNA gene silencing miRNA nuclear export HASTY miRNA biogenesis

Hexachlorobenzene Episode in Turkey

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Biomedical and Environmental Sciences 1989年第1期2卷 36-43页

作者： AYHAN GOCMEN HENRY A.PETERS derek j.CRIPPS GEORGE T.BRYAN CARL R.MORRIS Department of Pediatrics School of Medicine University of Hacettepe 06100 Ankara Turkey Center for Health Sciences University of Wisconsin Madison Wisconsin 53706 International Chemical Consultants Alexandria Virginia 22312

During the period 1955-1959, approximately 4000 people in southeast Anatolia developed porphyria due to the ingestion of hexachlorobenzene (HCB), a fungicide added to wheat seedlings. These HCB exposures subsequently led to the development of bullae on sun-exposed areas, hyperpigmentation, hypertrichosis, and porphyrinuria. The condition was called kara yara or 'black sore.' Many of the breast-fed children under the age of 2 years whose mothers had ingested HCB-treated grain died from a disease known as pembe yara or 'pink sore.' In this follow-up study of 252 patients, 20-30 years postexposure, there were 162 males and 90 females, with an average current age of 35.7 years, an average of onset of 7.6 years, and a duration of 2.2 years. Many patients had dermatologic, neurologic, and orthopedic symptoms and signs. The observed clinical findings include scarring of the face and hands (83.7％), hyperpigmentation (65％), hypertrichosis (44.8％), pinched facies (40.1％), painless arthritis (70.2％), small hands (66.6％), sensory shading (60.6％), myotonia (37.9％), cogwheeling (41.9％), enlarged thyroid (34.9％), and enlarged liver (4.8％). Urine and stool porphyrin levels were determined in all patients, and 17 have at least one of the porphyrins elevated. A total of 56 specimens of human milk obtained from mothers with porphyria were analyzed for HCB. The average value was 0.51 ppm in HCB-exposed patients compared to 0.07 ppm in unexposed controls. Offspring of mothers with three decades of HCB-induced porphyria appear normal. 1989 Academic Press, Inc.

关键词： Hexachlorobenzene Episode in Turkey HCB