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Effects of genistein, daidzein and glycitein on the osteogenic and adipogenic differentiation of bone marrow stromal cells and on the adipogenic trans-differentiation of osteoblasts

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Progress in Natural science:Materials International 2005年第11期15卷 993-999页

作者： LI Xianghui, ZHANG Jinchao, SUI Senfang and YANG Mengsu (State Key Laboratory of Biomembrane, department of Biological sciences and Biotechnology, Tsinghua university, Beijing 100084, china department of chemistry, college of chemistry & environmental science, hebei university, baoding 071002, china department of Biology and chemistry, City university of Hong Kong, Hong Kong, china) State Key Laboratory of Biomembrane Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China department of chemistry College of Chemistry and Environmental Science Hebei University Baoding 071002 China department of Biology and chemistry City University of Hong Kong Hong Kong Hong Kong

The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), alkaline phosphatase (ALP) activity and oil red O assays were used to examine the effects of genistein, daidzein and glycitein on the osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells (MSCs) and the adipogenic trans-differentiation of primary mouse osteoblasts. The results indicated that daidzein, genistein and glycitein at concentrations from 1×10-8 mol/L to 1×10-5mol/L promoted the proliferation of MSCs and osteoblasts; genistein, daidzein and glycitein promoted osteogenic differentiation and inhibited adipogenic differentiation of MSCs, and inhibited adipocytic transdifferentiation of osteoblasts at appropriate concentrations as 17β-estradiol. It suggests that genistein, daidzein and glycitein regulate a dual differentiational process of MSCs into the osteogenic and adipogenic lineages, and trans-differentiational process of primary osteoblasts into the adipocyte lineages, causing a lineage shift toward osteoblast. Protective effects of them on bone may be mediated by a reversal of adipogenesis which may promote the proliferation, differentiation and mineralization of osteoblasts, and make adipocytes secrete less cytokines which may promote osteoclast formation and activation. In addition, the results also indicated that genistein, daidzein and glycitein may be helpful in preventing the development of steroid induced osteonecrosis.

关键词： genistein daidzein glycitein bone marrow stromal cells osteoblasts osteogenic differentiation adipogenic differentiation adipogenic trans-differentiation.

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Effect of daidzin,genistin,and glycitin on osteogenic and adipogenic differentiation of bone marrow stromal cells and adipocytic transdifferentiation of osteoblasts

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Acta Pharmacologica Sinica 2005年第9期26卷 1081-1086页

作者： Xiang-hui LI~1 Jin-chao ZHANG~(2,3) Sen-fang SUI~(1,4) Meng-su YANG~(3,4)1 State Key Laboratory of Biomembrane department of Biological sciences and Biotechnology,Tsinghua university,Beijing 100084,china 2 department of chemistry college of chemistry and environmental science,hebei university,baoding 071002,china 3 department of Biology and chemistry,City university of Hong Kong,Hong Kong,china department of Biological sciences and Biotechnology State Key Laboratory of Biomembrane Tsinghua University Beijing China department of chemistry College of Chemistry and Environmental Science Hebei University Baoding China department of Biology and chemistry City University of Hong Kong Hong Kong China

Aim:To examine the effect of daidzin,genistin,and glycitin on the osteogenic andadipogenic differentiation of bone marrow stromal cells（MSC）and the adipogenictransdifferentiation of ***:MTT test,alkaline phosphatase（ALP）activity measurement,Oil Red O stain and measurement were ***:Daidzin,genistin,and glycitin 1×10-8,5×10-7,1×10-6,5×10-6,and 1×10-5mol/L all promoted the proliferation of primary mouse bone MSC and *** 5×10-7mol/L and genistin 1×10-6mol/L promoted the osteogenesis *** 1×10-8,5×10-7,1×10-6,5×10-6,and 1×10-5mol/L and glycitin 1×10-8,1×10-6,and 1×10-5mol/L inhibited the adipogenesis of ***,genistin,and glycitin 1×10-8,5×10-7,1×10-6,5×10-6,and 1×10-5mol/L all inhibited the adipocytictransdifferentiation of ***:Daidzin,genistin,and glycitinmay modulate differentiation of MSC to cause a lineage shift toward the osteo-blast and away from the adipocytes,and could inhibit adipocytic transdifferen-tiation of *** could also be helpful in preventing the developmentof osteonecrosis.

关键词： daidzin genistin glycitin bone marrow stro-real cells osteogenic differentiation adipogenic differentiation osteoblasts adipocytic transdifferentiation

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Effects of lanthanum and gadolinium on proliferation and differentiation of primary osteoblasts

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Progress in Natural science:Materials International 2007年第5期17卷 618-623页

作者： Zhang Dawei , Zhang Jinchao , Chen Yao , Yang Mengsu and Yao Xinsheng (department of Natural Products chemistry, Shenyang Pharmaceutical university, Shenyang 110016, china Shenzhen Research Institute of City university of Hong Kong, Shenzhen 518057, china department of Biology and chemistry, City university of Hong Kong, china department of chemistry, college of chemistry & environmental science, hebei university, baoding 071002, china Key Laboratory for Research and Development of Traditional Chinese Medicine and Natural Medicine at Shenzhen, Shenzhen 518057, china) department of Natural Products chemistry Shenyang Pharmaceutical University Shenyang 110016 China Shenzhen Research Institute City University of Hong Kong Shenzhen 518057 Hong Kong department of Biology and chemistry City University of Hong Kong Hong Kong Hong Kong department of chemistry College of Chemistry and Environmental Science Hebei University Baoding 071002 China Key Lab. for R and D of Traditional Chinese Medicine and Natural Medicine at Shenzhen Shenzhen 518057 China

The effects of La3+ and Gd3+ on the proliferation, differentiation and adipogenic transdifferentiation of rat calvarial osteoblast-like cells (ROB cells) were evaluated by MTT method, measuring the activity of alkaline phosphatase (ALP) and Oil red O measurement. Both of La3+ and Gd3+ inhibited the proliferation of ROB cells at all concentrations (1×10-5, 1×10-6, 1×10-7, 1×10-8, 1×10-9 mol·L-1). La3+ at concentration of 1×10-5mol·L-1 significantly increased the alkaline phosphatase activity of ROB cells up to 3 folds (P<0.01). However, the effects reversed to inhibit at other concentrations. Gd played a negative role in the alkaline phosphatase activity. La3+ inhibited the adipogenic transdifferentiation of ROB cells at all concentrations in a dose-dependent way. However, Gd promoted the adipogenic transdifferentiation of ROB cells at 1×10 and 1×10 mol·L . These findings suggested that the effects of rare earth elements on the proliferation, differentiation and adipogenic transdifferentiation of ROB cells were dependent on their concentrations and species.

关键词： lanthanum gadolinium osteoblast-like cells proliferation differentiation transdifferentiation.

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Surface modification of hydrophobic NaYF_4:Yb,Er upconversion nanophosphors and their applications for immunoassay

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science china chemistry 2011年第8期54卷 1292-1297页

作者： LIU ChengHui, WANG Zhe, WANG XiaoKe & LI ZhengPing Key Laboratory of Medicinal chemistry and Molecular Diagnosis, Ministry of Education college of chemistry and environmental science, hebei university, baoding 071002, china Key Laboratory of Medicinal chemistry and Molecular Diagnosis Ministry of Education College of Chemistry and Environmental Science Hebei University Baoding China

β-NaYFa：Yb,Er upconversion nanophosphor （UCNP） is known as one of the most efficient NIR-to-visible upconversion mate- dais, which shows great potential in bioanalytical chemistry and bioimaging. However, its applications are greatly limited due to its low water dispersibility and thus poor biocompatibility. In this paper, poly（acrylic acid）（PAA）-based ligand exchange strategies are carded out to modify oleic acid-capped hydrophobic β-NaYF4：Yb,Er UCNPs into hydrophilic ones. After efficient surface modification, the presence of free carboxylic acid groups on the surfaces of UCNPs results in high solubility in water, and also allows further conjugation with NH2-containing biomolecules. Facilitated by the covalent linkage between the -COOH groups on UCNPs surfaces and -NHz groups in antigen/antibody, a sensitive immunosensor is constructed by using PAA-functionalized β-NaYFa：Yb,Er UCNPs as biolabels. Through monitoring the upconversion fluorescence intensity or fluorescent imaging of the final immunocomplexes, high sensitivity is achieved for the proposed immunoassay and as low as 0.1 ng/mL goat anti-human immunoglobulin G （IgG） can be detected, which suggests that PAA-modified UCNPs may serve as an ideal candidate for use as bioanalysis and bioimaging probes.

关键词： surface modification upconversion nanophosphor ligand exchange immunoassay bioimaging

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Kinetic study on the ligand exchange reactions between bis(alkylxanthato)palladium(II)and bis(N,N-dialkyldithiocarbamato)palladium(II)by high-performance liquid chromatography

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Chinese Journal of chemistry 1992年第1期10卷 26-33页

作者： SHEN,Shi-Gang DING,Tian-Hui WANG,An-Zhou department of chemistry,hebei university,baoding 071002 department of chemistry Hebei University Baoding 071002

The rate constants and equilibrium constants of ligand exchange reactions between his (alkylxanthato)palladium(Ⅱ),Pd(S_2COR)_2(R=Am,n-Hex,Bz),and bis(N,N-dialkyldithiocarbamato) palladium(Ⅱ),Pd(S_2CNR_2)_2(K=Et,n-Pr,n-Bu),in chloroform solution have been determined in a temperature range of 20—50°C by means of high-performance liquid *** was found that both the forward and reverse reactions are of second *** of the equilibrium constants K determined are 10.3±1.0,much greater than the value(K=4)calculated statistically which indicates that the ternary complexes are more stable than the binary *** experimental results revealed that the reaction rate decreases with the increase in the size of R and R^1 groups and the latter are more remarkable,consistent with the deduction of steric *** activation parameters of the reactions have been *** reaction series(11)and(12)(in the text)the isokinetic tempera- tures B=390±8K and β=346±15 K have been observed respectively.A plausiblemechanism in- volving an eight-membered ring intermediate has been proposed on the basis of experimental results.

关键词： alkylxanthato)palladium Kinetic study on the ligand exchange reactions between bis N,N-dialkyldithiocarbamato)palladium high

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On the Duality Mapping Sets in Orlicz Sequence Spaces

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Acta Mathematica Sinica,English Series 2001年第4期17卷 595-602页

作者： Bao Xiang WANG department of Mathematics, hebei university, baoding 071002, P. R. china department of Mathematics Hebei University Baoding 071002 P. R. China

The criteria for the weak compactness of duality mapping sets J(x)={f∈X~*:〈f,x〉= ‖f‖~2=‖x‖~2}in Orlicz sequence spaces endowed either with the Luxemburg norm or with the Orlicz norm are obtaned.

关键词： Orlicz sequence spaces Duality mapping Weak compactness

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Immobilized chitosan as a selective absorbent for the nickel removal in water sample

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Journal of environmental sciences 2003年第5期15卷 633-638页

作者： WU Jian min, WANG Yong yao (department of chemistry, college of science, Zhejiang university, Hangzhou 310027, china. ) department of chemistry College of Science Zhejiang University Hangzhou 310029

Method for preparation of chitosan immobilized on silica gel(CTS silica) was described. The CTS silica was used as absorbent for the absorption of nickel in water. The results showed that this absorbent had relatively high selectivity and strong affinity to nickel. The maximum absorption capacity for nickel can reach 667 mg/g of chitosan. Factors that affect the absorption capacity, such as pH, ion strength and the presence of calcium, EDTA and the mechanism of absorption were discussed in detail. The absorbent can be regenerated with acid and reused for several times. The recovery rate for nickel can reach 99 99%. This absorbent filled in a column can be used in nickel removal from wastewater and drinking water.

关键词： immobilized chitosan nickel wastewater treatment

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The Smoothness of Scattering Operators for Sinh-Gordon and Nonlinear Schrodinger Equations

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Acta Mathematica Sinica,English Series 2002年第3期18卷 549-564页

作者： Bao Xiang WANG department of Mathematics, hebei university. baoding 071002. P. R. china department of Mathematics Hebei University Baoding P. R. China

We show that the scattering operator carries a band in H^s (R^n)×H^(s-1)(R^n) into H^s (R^n)× H^(s-1)(R^n) for the sinh-Gordon equation and an analogous result also holds true for the nonlinear Schrdinger equation with an exponential nonlinearity, where s≥n/2 is arbitrary and n≥2. Therefore, the scattering operators are infinitely smooth for the above two equations.

关键词： Sinh-Gordon equation Nolinear Schrodinger equation The smoothness of scattering operator

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SYNTHESIS AND PROPERTIES OF NEW MESOGEN-JACKETED LIQUID CRYSTALLINE POLYMERS

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Chinese Journal of Polymer science 2000年第2期18卷 139-148页

作者： Qi-ding Mi Qi-feng Zhou department of Polymer science & Engineering, college of chemistry Peking university, Beijing 100871, china 北京大学北京

Some new mesogen-jacketed liquid crystalline polymers (MJLCP) with polymer backbones, spacers, and mesogenic units of different structures were synthesized by radical polymerization. The mesomorphic behavior of these polymers was examined using differential scanning calorimetry (DSC) and polarizing optical microscopy (POM). Their liquid crystallinity is influenced by the variation of polymer backbone, spacer, mesogenic unit and its terminal groups. The results show that 1) a more flexible polymer main-chain is more favorable to the formation of a liquid crystal phase, while 2) a flexible spacer will decrease the 'Jacket Effect' and the liquid crystallinity and 3) a subtle modification of the terminal groups on the mesogenic unit may also have a significant influence on properties of the polymers.

关键词： mesogen-jacketed liquid crystalline polymers polymer backbones spacers mesogenic units liquid crystallinity

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Grassmann geometry on symmetric spaces

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Chinese science Bulletin 1999年第1期44卷 41-44页

作者： JIN Quanqindepartment of Mathematics, hebei university, baoding 071002, china department of Mathematics, Nankai university, Tianjin 300071,china department of Mathematics Hebei University Baoding China department of Mathematics Nankai University Tianjin China

A duality principle between Grassmann geometries on compact symmetric spaces and those on noncompact symmetric spaces is proved, which greatly facilitates the study of Grassmann geometry on symmetric spaces. Those Gra... 详细信息

关键词： symmetric spaces Grassmann geometry Cartan decomposition orthogonal symmetric Lie algebra totally geodesic submanifold.

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